Journal of Pharmacy and Pharmacology最新文献

{"title":"Anticancer potential of PEDF peptides.","authors":"Enas Bani-Ahmad, Joshua Dass, Crispin R Dass","doi":"10.1093/jpp/rgaf043","DOIUrl":"https://doi.org/10.1093/jpp/rgaf043","url":null,"abstract":"<p><strong>Objectives: </strong>Pigment epithelium-derived factor (PEDF) has demonstrated a wide range of activities, the most notable of which is its role in cancer.</p><p><strong>Methods: </strong>Articles were sourced from Scopus with the following keywords-PEDF, peptide(s), cancer, tumour, and tumour. There was no limit set on date of publication, and the language of publication was set to English.</p><p><strong>Key findings: </strong>Researchers have found two functional epitopes in the PEDF sequence: a 34-mer peptide that mainly inhibits angiogenesis and a 44-mer peptide that mainly promotes differentiation and neurotrophic functions in certain cell lines. Furthermore, studies have demonstrated that shorter peptides in the 34-mer significantly contribute to its angiogenic activity. PEDF peptide functions as an anticancer agent through various mechanisms. The most salient feature is the blockade of angiogenesis by reducing VEGF levels. Angiogenesis is critical in tumour expansion, and it is known as the process whereby new blood vessels are formed from capillaries.</p><p><strong>Conclusions: </strong>Researchers have studied several PEDF peptides in various types of cancer, including ovarian, breast, lung, osteosarcoma, and myeloma. This underscores the potential significance of the various PEDF peptides, given their known ability to influence angiogenesis and other biological processes.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of polyphenolic-enriched Pleurospermum candollei extract against doxorubicin-induced cardiovascular disease in rats: modulation of oxidative stress, inflammation, and apoptosis.","authors":"Mehreen Fatima, Muhammad Rashid Khan, Lamya Ahmed Al-Keridis, Nawaf Alshammari, Mitesh Patel, Mohd Adnan","doi":"10.1093/jpp/rgaf042","DOIUrl":"https://doi.org/10.1093/jpp/rgaf042","url":null,"abstract":"<p><strong>Objectives: </strong>Pleurospermum candollei is used to treat abdominal problems, heart diseases, gastric issues, and cerebral disorders. This study aimed to evaluate the therapeutic potential of P. candollei aqueous extract (PCA) against Doxorubicin (DOX)-induced cardiac failure in rats.</p><p><strong>Methods: </strong>The composition of the extract was determined by quantifying polyphenols using HPLC-DAD, and antioxidant capacity was evaluated through the FRAP assay. Fifty-four rats were divided into nine groups, and cardiac injury was induced by DOX (10 mg/kg body weight). PCA extract (200, 400, and 600 mg/kg body weight) was administered orally to treat cardiotoxicity. Serum markers, antioxidants, inflammatory, fibrotic, and apoptotic genes, and histological alterations were assessed.</p><p><strong>Key findings: </strong>Examination confirmed the presence of various polyphenols in the plant extract. PCA extract administration to rats reduced the DOX-instigated elevation in CK, LDH, and triglycerides concentrations in serum and restored the histopathological changes. Similarly, PCA extract administration normalized the levels of antioxidants and pro-inflammatory markers and reduced the expression of apoptotic and fibrosis markers.</p><p><strong>Conclusion: </strong>PCA extract exhibited significant antioxidants and cardioprotective activities in rats. The cardioprotective and anti-inflammatory effects of PCA may be attributed to its high content of polyphenolics, and it acts as a promising therapeutic source against heart failure.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the neuroprotective role of Synta-66 in type-2 diabetes mellitus-induced dementia in rats.","authors":"Ashi Mannan, Maneesh Mohan, Shareen Singh, Sonia Dhiman, Thakur Gurjeet Singh","doi":"10.1093/jpp/rgaf036","DOIUrl":"https://doi.org/10.1093/jpp/rgaf036","url":null,"abstract":"<p><strong>Objectives: </strong>This study explores the potential inhibitory effects of Synta-66 at doses of 1 and 5 mg/kg, with a particular emphasis on the role of ORAI-I in amyloidogenesis, a common mechanism that underlies type-2 diabetes mellitus (T2DM) and Alzheimer's disease (AD).</p><p><strong>Methods: </strong>Induction of T2DM-induced AD by the high-fat diet (HFD)-Streptozotocin (STZ)-Aβ25-35 model. Assessment of behavioral parameters like polydipsia, polyphagia, Morris water maze, and passive avoidance test; biochemical estimation of glucose, insulin, oxidative stress (superoxide dismutase (SOD), glutathione (GSH), catalase (Cat), and thiobarbituric acid reactive substances (TBARS)), neuroinflammation (interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), and nuclear factor-kappa B (NF-κβ)), Aβ level, through ELISA technique, and calcium levels via atomic absorption spectrometer.</p><p><strong>Key findings: </strong>Synta-66 (5 and 10 mg/kg) results in a reduction in food and water intake, as well as a reduction in memory impairment in the Morris water maze and passive avoidance test. Furthermore, it normalizes glucose, insulin, and antioxidant elements (SOD, GSH, and Cat) level, while decreasing TBARS levels. In addition, ELISA data demonstrated a reduction in neuroinflammation (downregulation of IL-1β, IL-6, TNF-α, and NF-κβ), Aβ accumulation, and calcium levels by Synta-66 (5 and 10 mg/kg).</p><p><strong>Conclusion: </strong>Consequently, ORAI can play a crucial role in the mediation of amyloidogenesis induced by T2DM, thereby establishing a connection between T2DM and AD. Therefore, Synta-66 has the potential to treat and prevent the progression of T2DM to AD.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the in vitro, in vivo anti-inflammatory potential of Ocimum basilicum and in silico analysis of its phytocompounds targeting COXs proteins.","authors":"Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf","doi":"10.1093/jpp/rgaf032","DOIUrl":"https://doi.org/10.1093/jpp/rgaf032","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the anti-inflammatory potential of ethanolic extract of Ocimum basilicum seeds (EEOBS) through in vitro, in vivo, and in silico approaches.</p><p><strong>Methods: </strong>The in vivo anti-inflammatory activity of EEOBS was assessed using a carrageenan-induced paw edema model in Swiss albino mice, where paw thickness was measured at 1, 2, 3, 4, and 5 hours post-treatment. The in vitro anti-inflammatory potential was evaluated using a bovine serum albumin (BSA) denaturation assay at varying concentrations of EEOBS (50, 100, 250, 500, and 1000 μg/ml).</p><p><strong>Key findings: </strong>Gas chromatography-mass spectrometry (GC-MS) analysis of EEOBS revealed the presence of several bioactive phytochemicals, with 9,12,15-Octadecatrienoic acid (47.27%) and hexadecanoic acid (13.45%) as the major constituents. Histopathological analysis of mice paws showed significant restoration of the keratin and epithelium layers in treated groups compared to the control. Molecular docking analysis identified linoleic acid and 12-Z-octadecatrienoic acid as the most promising compounds, demonstrating higher binding affinity than the standard inhibitor for both cyclooxygenase proteins (COX-1: PDB ID 1EQG and COX-2: PDB ID 1CX2). Additionally, n-octadecanoic acid exhibited superior binding with COX-2 (1CX2).</p><p><strong>Conclusion: </strong>The in vitro, in vivo, and in silico findings suggest that EEOBS possesses significant anti-inflammatory potential, indicating its suitability for targeted anti-inflammatory therapies. However, further clinical trials are required to validate its therapeutic efficacy.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rb1 inhibits chronic stress-induced colorectal cancer via regulating glycolysis and β2-AR/CREB1 signaling pathway.","authors":"Wang Yao, Dong-Ming Hua, Wen-Kai Wang, Zhao-Zhou Zhang, Yun-Feng Guan, Yan Wang","doi":"10.1093/jpp/rgaf031","DOIUrl":"https://doi.org/10.1093/jpp/rgaf031","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism of ginsenoside Rb1 (G-Rb1) against colorectal cancer under chronic stress.</p><p><strong>Methods: </strong>A chronic restraint stress (CRS) model and a colorectal cancer (CRC) subcutaneous xenograft model were established. Western blot analysis quantified β2-adrenergic receptor (β2-AR), cAMP response element-binding protein 1 (CREB1), and p-CREB1 expression. Additionally, glycolytic enzymes GLUT1, HK2, and PFKP were analyzed via Western blot and RT-qPCR, with glucose uptake, lactate, ATP, and stress hormone levels assessed by flow cytometry, kits, and ELISA.</p><p><strong>Key findings: </strong>Compared to the control group, the stress group exhibited increased tumor volume and mass, along with elevated expression of β2-AR, p-CREB1, and upregulated expression levels of GLUT1, HK2, and PFKP. Additionally, glucose, lactate, and epinephrine levels were higher in the stress group. In comparison to the stress group, G-Rb1 treatment demonstrated reduced tumor volume and mass, decreased p-CREB1 expression, as well as downregulated protein and mRNA levels of GLUT1, HK2, and PFKP. Glucose, lactate, and epinephrine levels also showed a reduction in the G-Rb1-treated groups.</p><p><strong>Conclusions: </strong>G-Rb1 suppresses the growth of colorectal cancer under chronic stress, potentially through downregulation of the β2-AR/CREB1 signaling pathway, thereby reducing glycolytic activity in colorectal cancer under chronic stress.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the molecular mechanism of Taohong Siwu decoction in the treatment of non-small-cell lung cancer based on network pharmacology and molecular docking.","authors":"Yuan Qin, Jia-Ning Lian, Xin Chen, Feng-Yu Huang, Hai-Wen Chen, Tai-Wei Dong, Zuo-Lin Jin","doi":"10.1093/jpp/rgae141","DOIUrl":"10.1093/jpp/rgae141","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore the mechanism of Taohong Siwu decoction (THSWD) in the treatment of non-small-cell lung cancer (NSCLC) by using comprehensive analysis.</p><p><strong>Methods: </strong>The active components and relevant targets of THSWD were analyzed by network analysis to construct the active component-target-disease network diagram. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted on the core targets by the Metascape database. Molecular docking verification was used for molecular visualization.</p><p><strong>Key findings: </strong>A total of 69 active compounds and 114 targets were filtered in lung cancer treatment with THSWD. KEGG analysis suggested that tumor necrosis factor (TNF) signaling pathway, and apoptosis pathway played critical roles. The results of molecular docking showed that populoside_qt with IL-6, baicalein with epidermal growth factor receptor (EGFR), and luteolin with MAPK8 had the strongest binding ability. Moreover, experiment validation revealed that THSWD regulated the expression of IL-6, AKT, Cyclin D1, E-cadherin, and LC3A/B, thereby inhibiting the proliferation and migration ability, promoting apoptosis, and blocking the cell cycle of NSCLC cells.</p><p><strong>Conclusions: </strong>The potential targets and molecular mechanisms of THSWD in the treatment of NSCLC were preliminarily revealed by a comprehensive analysis in this study, which will provide new ideas and methods for the study of the mechanism of traditional Chinese medicine in treating lung cancer.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"805-821"},"PeriodicalIF":2.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142836980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piperlongumine inhibits glioblastoma proliferation by inducing ferroptosis.","authors":"Jianting Qiu, Fangzhou Guo, Ji Shi, Tangjun Guo, Haozhe Piao","doi":"10.1093/jpp/rgae148","DOIUrl":"10.1093/jpp/rgae148","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the effects of Piperlongumine on Glioblastoma multiforme.</p><p><strong>Methods: </strong>The effects of Piperlongumine on the viability and proliferation of glioma cells LN229 and A172 were measured. Changes in mitochondrial structure were observed. Cell proliferative capacity was assessed using immunofluorescence. The levels of glutathione, malondialdehyde, 4-hydroxynonenal, and intracellular reactive oxygen species were detected. The levels of ferroptosis-related proteins were detected. A plasmid transfection was performed to overexpress the nuclear factor erythroid 2-related factor 2 gene; a subcutaneous tumor model was established in nude mice to observe the in vivo inhibitory effects of Piperlongumine on Glioblastoma multiforme and the recovery effect of Fer-1. The expression levels of ferroptosis-related proteins were detected using immunohistochemistry.</p><p><strong>Key findings: </strong>Piperlongumine inhibited the viability of glioma cells, as well as their proliferation. The ferroptosis inhibitors were able to restore the inhibitory effect of Piperlongumine on glioma cell proliferation. Forced overexpression of nuclear factor erythroid 2-related factor 2 partially reversed Piperlongumine-induced ferroptosis; Piperlongumine exhibited a significant inhibitory effect on Glioblastoma multiforme cells in vivo, which could be restored by Fer-1.</p><p><strong>Conclusions: </strong>Piperlongumine inhibits Glioblastoma multiforme proliferation by inducing ferroptosis in vitro and vivo model.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"822-833"},"PeriodicalIF":2.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PD15, a steroidal saponin, induces apoptosis of HCT116 colorectal cancer cells via suppressing the Akt/GSK3β pathway.","authors":"Minna Yao, Yi Ding, Yang Sun, Kai Gao, Ruili Li, Wei Zhang, Weiwei Li, Yanhua Wang, Yi Qiao, Haifeng Tang, Jingwen Wang","doi":"10.1093/jpp/rgae151","DOIUrl":"10.1093/jpp/rgae151","url":null,"abstract":"<p><strong>Objectives: </strong>PD15, a novel natural steroidal saponin extracted from the rhizomes of Paris delavayi Franchet, has demonstrated a strong cytotoxic effect against HepG2 and U87MG cells. However, its therapeutic effects on colorectal cancer (CRC) and the underlying molecular mechanisms remain unclear.</p><p><strong>Methods: </strong>MTT assay, clonogenic assay, Hoechst 33258 staining, flow cytometry, molecular docking, and western blot were used to investigate the mechanism of PD15 in HCT116 cell lines. Additionally, the anti-CRC effects of PD15 were evaluated in vivo using HCT116 xenograft models.</p><p><strong>Key findings: </strong>PD15 significantly inhibited cell proliferation and induced G0/G1 phase arrest in HCT116 cells. Furthermore, PD15 upregulated cleaved Caspase 3 and 9, cleaved PARP, and Bax expression levels while downregulating Bcl-2, leading to apoptosis. Further experiments revealed that PD15 downregulated the protein expression of p-Akt and p-GSK3β, with LY294002 (a PI3K/Akt inhibitor) enhancing PD15-induced apoptosis and its effects on Akt/GSK3β-associated proteins. In addition, molecular docking demonstrated that PD15 exhibited strong binding affinity with Akt and GSK3β. Critically, PD15 inhibited CRC growth in vivo without causing apparent toxicity in mice.</p><p><strong>Conclusions: </strong>These findings indicate that PD15 could trigger apoptosis by suppressing the Akt/GSK3β signaling pathway in HCT116 cells.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"834-844"},"PeriodicalIF":2.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dihydroisotanshinone I regulates ferroptosis via PI3K/AKT pathway to enhance cisplatin sensitivity in lung adenocarcinoma.","authors":"Feng-Jiao Li, Li-Chen Gao, Hui-Zhi Long, Zi-Wei Zhou, Hong-Yu Luo, Shuo-Guo Xu, Shang-Ming Dai, Jin-Da Hu","doi":"10.1093/jpp/rgae085","DOIUrl":"10.1093/jpp/rgae085","url":null,"abstract":"<p><strong>Objectives: </strong>Dihydroisotanshinone I (DT) is a kind of diterpenoid compound extracted from the dried roots of Salvia miltiorrhiza Bunge, and exhibits multiple biological activities including anti-tumor activity. Cisplatin is one of the first-line drugs for the treatment of lung adenocarcinoma (LAUD), but the drug resistance and toxicity limit its efficacy. DT is known to induce apoptosis and ferroptosis, but it is unclear whether DT can inhibit the cisplatin-resistant LAUD cells and reverse the drug resistance in LAUD. Therefore, our study intends to establish the cisplatin-resistant human LAUD cells (A549/DDP), and figure out the influence and related mechanisms of DT reversing cisplatin resistance in A549/DDP cells, so as to provide a theoretical basis for the DT as a new natural candidate for the treatment of LAUD.</p><p><strong>Methods: </strong>The establishment of A549/DDP was the continuous stimulation by exposing A549 to gradient concentrations of Cisplatin. The cell viability of A549 and A549/DDP was detected by CCK-8 kit, and the IC50 value was calculated. The morphological changes of A549 and A549/DDP cells were observed by an inverted microscope. The contents of malondialdehyde (MDA) and glutathione (GSH) in A549/DDP cells after drug treatment were detected by related kits. The levels of Fe2+, cytosolic reactive oxygen species (ROS), and lipid reactive oxygen species (lipid ROS) were detected by a fluorescence microplate reader or fluorescence cell imager according to the related fluorescent probe kit instructions. Western blot was used to detect the expressions of PI3K, phospho-PI3K, AKT, phospho-AKT, MDM2, p53, GPX4, and SLC7A11 in A549/DDP after different drug treatments.</p><p><strong>Key findings: </strong>Our study demonstrated that the inhibitory effect of DT on A549 and A549/DDP cells was time-dependent and concentration-dependent, and DT and DDP had a synergistic effect on inhibiting the proliferation of A549/DDP cells. Furthermore, DT mainly induced ferroptosis in A549/DDP cells and synergized with cisplatin to promote ferroptosis in A549/DDP cells. The result of KEGG pathway analysis, molecular docking and western blot showed that DT could enhance the cisplatin sensitivity of A549/DDP by inhibiting PI3K/MDM2/P53 signaling pathway.</p><p><strong>Conclusions: </strong>Consequently, we concluded that DT promotes ferroptosis in cisplatin-resistant LAUD A549/DDP cells. Additionally, DT reverses cisplatin resistance by promoting ferroptosis via PI3K/MDM2/P53 pathway in A549/DDP cells.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"752-767"},"PeriodicalIF":2.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The therapeutic potential of curculigoside in poststroke depression: a focus on hippocampal neurogenesis and mitochondrial function.","authors":"Ning-Xi Zeng, Xin Chen, Xiao-Yan Yang, De-Sheng Chen, Mei Shen","doi":"10.1093/jpp/rgae091","DOIUrl":"10.1093/jpp/rgae091","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects and mechanism of curculigoside against poststroke depression (PSD).</p><p><strong>Methods: </strong>In vivo, a PSD rat model was created by combining bilateral common carotid artery occlusion and chronic unpredictable mild stress stimulations. After 4-week modeling and intragastrically administration of curculigoside, the effects of curculigoside on behavior, hippocampal neurogenesis, and hippocampal mitochondrial oxidative phosphorylation (OxPhos) were investigated. In vitro, PSD-like primary neural stem cells (NSCs) model was established by oxygen-glucose deprivation/recovery (OGD/R) combing high-corticosterone (CORT) concentration, followed by treatment with curculigoside. The investigation subsequently examined the impact of curculigoside on mitochondrial OxPhos, proliferation, and differentiation of NSCs under OGD/R + CORT conditions.</p><p><strong>Key findings: </strong>In vivo, PSD rats showed significantly depressive behaviors, dysfunctional neurogenesis in hippocampus, as well as decreased hippocampus adenosine triphosphate (ATP) levels, reduced electron transport chain complexes activity, and downregulates mitochondrial transcription factor A (TFAM) and PPAR-gamma coactivator 1 alpha (PGC-1α) expression in hippocampus. In vitro, OGD/R +CORT significantly injured the proliferation and differentiation, as well as impaired the mitochondrial OxPhos in NSCs. Curculigoside treatment was effective in improving these abnormal changes.</p><p><strong>Conclusion: </strong>Curculigoside may repair hippocampal neurogenesis in PSD rats by enhancing hippocampal mitochondrial OxPhos, and has shown a great potential for anti-PSD.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"768-782"},"PeriodicalIF":2.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}