Journal of Poultry Science最新文献

{"title":"Effect of Dietary Carotenoid on Egg Yolk Color and Singlet Oxygen Quenching Activity of Laying Hens","authors":"S. Kojima, Sakura Koizumi, Yukari Kawami, Yuna Shigeta, Aya Osawa","doi":"10.2141/jpsa.0210032","DOIUrl":"https://doi.org/10.2141/jpsa.0210032","url":null,"abstract":"The effects of dietary carotenoids on egg yolk were investigated in this study. Forty Rhode Island Red (RR) and 40 Silky Fowl (SF) hens that were 60 weeks old were used. Hens of each breed were randomly divided into four dietary groups. One group was fed a basal diet (crude protein 17%, metabolizable energy 2800 kcal/kg) only, whereas the other groups received a specific additive, namely, paprika extract, marigold petal extract, or Paracoccus cell powder, in addition to the same basal diet. The color and carotenoid content of egg yolk and singlet oxygen quenching activity were measured after 4 weeks. The total carotenoid content, zeaxanthin content, and singlet oxygen quenching activity in the yolk differed significantly between breeds and between diets (two-way ANOVA). The lutein content in egg yolk was affected by breed and diet, as well as by the interaction between these two factors. Regarding the Roche Yolk Color Fan values, only the effect of diet was significant. In terms of objective egg yolk color, there was a significant difference in lightness and yellowness between breeds. The total carotenoid content was higher in SF than in RR in all the groups. Likewise, the levels of zeaxanthin and lutein in the yolk were higher in SF than in RR (P<0.05). The results of the present study suggest that dietary carotenoids are effective feed additives for laying hens, especially SF, to improve the color and singlet oxygen quenching activity of egg yolk.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"137 - 142"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47157382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Observed Incubation Behavior on Egg Production in Laying Hens of a Commercial Chicken Breed and Detection of Single-Nucleotide Polymorphisms Associated with the Incubation Behavior","authors":"Yuichiro Yonetani, Atsushi J. Nagano, H. Ueno, T. Amano","doi":"10.2141/jpsa.0210037","DOIUrl":"https://doi.org/10.2141/jpsa.0210037","url":null,"abstract":"Upon contact with laid eggs, avians initiate incubation behavior and stop laying additional eggs. This phenomenon suggests that the productivity of laying hens in free-range facilities may decrease because of frequent contact with laid eggs. Here, we examined whether hens of a commercial breed exhibit incubation behavior in a free-range facility and whether egg productivity subsequently decreases. One-hour observations were performed twice weekly for 3 weeks, during which 9 of 129 hens (7.0%) exhibited incubation behavior (i.e., sitting on eggs) in the free-range facility and were defined as incubating hens. During 4 d of continuous behavioral observation, incubating and non-incubating hens laid the same number of eggs statistically (4.6 and 3.6, on average, respectively); however, incubating hens spent significantly more time on average incubating the eggs (2071.9 min) than did the non-incubating hens (20.9 min; P<0.05), indicating a clear behavioral difference. Subsequently, the incubation behavior and egg productivity of incubating hens and a Silkie Fowl breed hen, which is known to exhibit typical incubation behavior and cessation of laying, were continuously compared for 27 d. The average minutes spent incubating eggs during the observation period increased in both the incubating hens and Silkie Fowl hen and the total time was almost the same (18,088.5 and 23,092 min, respectively). However, the Silkie Fowl hen stopped laying on day 17 after laying 17 eggs, whereas the incubating hens continued laying throughout the observation period. Incubating hens laid an average of 24.5 eggs, indicating that some hens (at least those of the commercial breed used in our study) can continue laying while exhibiting incubation behavior. A single-nucleotide polymorphism associated with incubation behavior was detected on chromosome 4 through genome-wide association analysis.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"121 - 128"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41556956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Corticosterone in Lipid Metabolism in Broiler Chick White Adipose Tissue","authors":"K. Honda, Kiyotaka Kurachi, Shoko Takagi, Takaoki Saneyasu, H. Kamisoyama","doi":"10.2141/jpsa.0210060","DOIUrl":"https://doi.org/10.2141/jpsa.0210060","url":null,"abstract":"Excessive accumulation of body fat in broiler chickens has become a serious problem in the poultry industry. However, the molecular mechanism of triglyceride accumulation in chicken white adipose tissue (WAT) has not been elucidated. In the present study, we investigated the physiological importance of the catabolic hormone corticosterone, the major glucocorticoid in chickens, in the regulation of chicken WAT lipid metabolism. We first examined the effects of fasting on the mRNA levels of lipid metabolism-related genes associated with WAT, plasma corticosterone, and non-esterified fatty acid (NEFA). We then examined the effects of corticosterone on the expression of these genes in vivo and in vitro. In 10-day-old chicks, 3 h of fasting significantly decreased mRNA levels of lipoprotein lipase (LPL) in WAT and significantly elevated plasma concentrations of NEFA. Six hours of fasting significantly increased mRNA levels of adipose triglyceride lipase (ATGL) in WAT and significantly elevated plasma concentrations of corticosterone. On the other hand, fasting significantly reduced mRNA levels of LPL in WAT and elevated plasma concentrations of NEFA in 29-day-old chicks without affecting mRNA levels of ATGL in WAT or plasma corticosterone concentrations. Oral administration of corticosterone significantly reduced mRNA levels of LPL and significantly increased the mRNA levels of ATGL in WAT in 29-day-old chicks without affecting plasma NEFA concentrations. The addition of corticosterone to primary chicken adipocytes significantly increased mRNA levels of ATGL, whereas mRNA levels of LPL tended to decrease. NEFA concentrations in the culture medium were not influenced by corticosterone levels. These results suggest that plasma corticosterone partly regulates the gene expression of lipid metabolism-related genes in chicken WAT and this regulation is different from the acute elevation of plasma NEFA due to short-term fasting.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"152 - 158"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41511183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method","authors":"Ryo Ezaki, Kennosuke Ichikawa, M. Matsuzaki, H. Horiuchi","doi":"10.2141/jpsa.0210067","DOIUrl":"https://doi.org/10.2141/jpsa.0210067","url":null,"abstract":"In chickens, primordial germ cells (PGCs) are effective targets for advanced genome editing, including gene knock-in. Although a long-term culture system has been established for chicken PGCs, it is necessary to select a gene-editing tool that is efficient and precise for editing the PGC genome while maintaining its ability to contribute to the reproductive system. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) and CRISPR-mediated precise integration into the target chromosome (CRIS-PITCh) methods are superior as the donor vector is easier to construct, has high genome editing efficiency, and does not select target cells, compared to the homologous recombination method, which has been conventionally used to generate knock-in chickens. In this study, we engineered knock-in chicken PGCs by integrating a fluorescent protein gene cassette as a fusion protein into the chicken vasa homolog (CVH) locus of chicken PGCs using the CRIS-PITCh method. The knock-in PGCs expressed the fluorescent protein in vitro and in vivo, facilitating the tracking of PGCs. Furthermore, we characterized the efficiency of engineering double knock-in cell lines. Knock-in cell clones were obtained by limiting dilution, and the efficiency of engineering double knock-in cell lines was confirmed by genotyping. We found that 82% of the analyzed clones were successfully knocked-in into both alleles. We suggest that the production of model chicken from the knock-in PGCs can contribute to various studies, such as the elucidation of the fate of germ cells and sex determination in chicken.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"182 - 190"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45404785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Estradiol on Expression of Estrogen Receptor and Collagen mRNAs in Chick Skin","authors":"S. Nishimura, Sayaka Arai, Mizuki Ohtani, Yuri Shimomura, S. Tabata","doi":"10.2141/jpsa.0210093","DOIUrl":"https://doi.org/10.2141/jpsa.0210093","url":null,"abstract":"Skin thickness and strength differ between male and female chickens. This study aimed to clarify the effects of estradiol on the expression of estrogen receptors and collagen mRNA in chicken skin. Estradiol was administered to male chicks for 3 weeks, then cryosections of skin collected from the cervical, thoracic, dorsal, and pelvic limb regions were stained with hematoxylin and eosin, and dermal thickness was measured. Estrogen receptor and collagen mRNA expression was assessed using real-time RT-PCR, and collagen contents were determined. Estradiol did not alter dermal thickness or the collagen content of the skin from any tested region. Among the estrogen receptors, significantly more ESR1 mRNA was expressed in the thoracic skin of chicks administered with estradiol compared with vehicle (control), and in the thoracic skin compared with skin from other regions within each group. Estradiol did not affect ESR2, GPER, and COL1A1 mRNA expression. These results suggested that estradiol stimulates ESR1 expression in thoracic skin, but does not affect collagen synthesis in skin from any other region of male chicks.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"162 - 167"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41917501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Detoxified Nano Sulfur Supplementation on the Growth, Nutrient Digestibility, Meat Quality, Excreta Microbes, Gas Emissions, and Blood Profiles of Broilers.","authors":"Md Raihanul Hoque,&nbsp;Insun Park,&nbsp;Seyoung Mun,&nbsp;In Ho Kim","doi":"10.2141/jpsa.0200111","DOIUrl":"https://doi.org/10.2141/jpsa.0200111","url":null,"abstract":"<p><p>A 35-day experiment was conducted to evaluate the effects of the supplementation of mineral detoxified sulfur dispersion ((DSD); Patent No.: 10-1997773) on the growth performance, meat quality, excreta microbiota, gas emissions, nutrient digestibility, and blood profiles of broilers. In total, 720 one-day-old ROSS 308 broilers, with an initial body weight of 41.9±0.8 g, were divided into two (2) treatment groups with 20 replicate pens/groups composed of 18 birds per pen. Treatments consisted of 1) CON (the control), normal drinking water and 2) TRT (the treatment group), CON+0.001% DSD (1000:1 dilution ratio). Average daily feed intake (ADFI) and feed conversion ratio (FCR) increased in the TRT group (<i>P</i><0.05) between days 1 to 7 and days 7 to 21 of the experimental period. Similarly, body weight gain (BWG) showed a significant increase (<i>P</i><0.05) in the DSD-supplemented group throughout in the length of the experiment. With regard to meat quality, redness (a*) was higher, while drip loss was lower, on the 7^th day in the DSD group. Furthermore, DSD supplementation increased (<i>P</i><0.05) <i>Lactobacillus</i> excreta but decreased <i>E. coli</i> concentrations in the TRT group compared to the CON group. Notably, nutrient digestibility, excreta gas emission, and blood profiles did not show any significant differences (<i>P</i>>0.05). DSD supplementation, administered through drinking water, has a positive impact on the growth performance, meat quality, and excreta microbiota of broiler chickens.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"48-55"},"PeriodicalIF":1.5,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/1e/59_48.PMC8791777.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin E and Selenium Given as Dietary Supplements Accumulate in Tissues and Semen and Improve Reproductive Parameters in Older Red Cornish.","authors":"Rosalie Adina Bălăceanu,&nbsp;Victor G Nimigean,&nbsp;Vanda Roxana E S Nimigean,&nbsp;Ştefania Raită,&nbsp;Laurenţ Ognean,&nbsp;Nicolae Dojană","doi":"10.2141/jpsa.0200069","DOIUrl":"https://doi.org/10.2141/jpsa.0200069","url":null,"abstract":"<p><p>The reproductive performance of broiler breeder chickens noticeably decreases toward the end of their commercial lives. Herein, we determined the effects of vitamin E and selenium dietary supplementation on semen traits, egg fertility (defined as fertilization and hatching rates) of adult (49-week-old) and older (63-week-old) Red Cornish breeders. We found that both vitamin E and selenium were concentrated in the liver and adipose tissue of adult and older Red Cornish breeders, and were transferred to the semen and egg yolk, respectively, in proportion to the level of supplementation. Vitamin E supplementation, in particular, improved ejaculate volume, total sperm count, sperm motility, and viability in both adult and older roosters, whereas selenium improved sperm motility and viability in the adult roosters. Egg fertility increased following supplementation with either vitamin E or selenium. The hatching rate also improved by both supplements in proportion to the level of supplementation. No significant synergistic effects of vitamin E and selenium were found. The levels of egg fertility and sperm trait improvements diminished with the age of the birds and depended on vitamin E and/or selenium doses. Thus, as dietary vitamin E and selenium supplements improved semen quality and egg fertility in these older Red Cornish broiler breeders, such birds could be maintained in flocks to prolong their reproductive output.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"96-103"},"PeriodicalIF":1.5,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c3/d3/59_96.PMC8791772.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Supplementation of Calcium Propionate and Calcium Butyrate Improves Eggshell Quality of Laying Hens in the Late Phase of Production.","authors":"Mengze Song,&nbsp;Hongchao Jiao,&nbsp;Jingpeng Zhao,&nbsp;Xiaojuan Wang,&nbsp;Haifang Li,&nbsp;Ping Wang,&nbsp;Baishun Ma,&nbsp;Shuhong Sun,&nbsp;Hai Lin","doi":"10.2141/jpsa.0200127","DOIUrl":"https://doi.org/10.2141/jpsa.0200127","url":null,"abstract":"<p><p>The aim of this study was to evaluate the effects of dietary supplementation of calcium propionate and calcium butyrate on the laying performance, eggshell quality, and expression of genes related to calcium and phosphorus metabolism in the tibia. One hundred and twenty 70-week-old Isa Brown hens were randomly assigned to three treatments, and each treatment had four replicates of 10 birds fed a basal diet (control) or a basal diet supplemented with 0.5% calcium propionate (CP) or 0.5% calcium butyrate (CB) for 8 weeks. The CB and CP treatments had no significant effect (<i>P</i>>0.05) on the laying rate, egg production, egg weight, and feed efficiency. The eggshell percentage was increased from week 2 (<i>P</i><0.05) and eggshell thickness was elevated at week 8 (<i>P</i><0.01) by both CP and CB treatments. Compared to the control treatment, the CB treatment increased serum calcium and phosphorus levels at week 4 (<i>P</i><0.05), whereas the CP and CB treatments decreased serum phosphorus at weeks 6 and 8, respectively (<i>P</i><0.05). Dietary supplementation had no effect on the bone index and bending strength of the tibia (<i>P</i>>0.05). The calcium and phosphorus content of the tibia was decreased by the CB treatment (<i>P</i><0.05). In the spleen, <i>NF-κB</i> and <i>IL-6</i> transcript levels were not influenced (<i>P</i>>0.05) but <i>TNF-α</i> transcript levels were decreased by the CP treatment (<i>P</i><0.05). In the tibia, the expression levels of <i>NF-κB, TNF-α</i>, and <i>IL-17</i> were not affected by the CP or CB treatment (<i>P</i>>0.05). The CP and CB treatments had no significant effect on the transcript levels of <i>RANKL</i>, <i>OPG, RNUX2, OPN, α-Clotho</i>, and <i>VDR</i> (<i>P</i>>0.05). In contrast, <i>PHEX</i> transcript levels were increased by the CP treatment (<i>P</i><0.05). The expression levels of <i>osteocalcin</i> (<i>P</i>=0.094) and <i>FGF23</i> (<i>P</i>=0.087) tended to decrease under the CB treatment. In conclusion, dietary supplementation of 0.5% calcium butyrate or 0.5% calcium propionate improved the eggshell quality of aged laying hens, possibly as a result of decreased deposition or enhanced mobilization of bone calcium and phosphorus.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"64-74"},"PeriodicalIF":1.5,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/53/59_64.PMC8791774.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm Motility Regulation in Male and Female Bird Genital Tracts.","authors":"Mei Matsuzaki,&nbsp;Tomohiro Sasanami","doi":"10.2141/jpsa.0200105","DOIUrl":"https://doi.org/10.2141/jpsa.0200105","url":null,"abstract":"<p><p>Sperm drastically change their flagellar movement in response to the surrounding physical and chemical environment. Testicular sperm are immotile; however, they gain the competence to initiate motility during passage through the male reproductive tract. Once ejaculated, the sperm are activated and promptly initiate motility. Unlike mammals, ejaculated sperm in birds are stored in specialized tubular invaginations referred to as sperm storage tubules (SSTs), located between the vagina and uterus, before fertilization. The resident sperm in the SSTs are in a quiescent state and then re-activated after release from the SSTs. It is thought that avian sperm can undergo motility change from quiescent to active state twice; however, the molecular mechanism underlying sperm motility regulation is poorly understood. In this short review, we summarize the current understanding of sperm motility regulation in male and female bird reproductive tracts. We also describe signal transduction, which regulates sperm motility, mainly derived from <i>in vitro</i> studies.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"1-7"},"PeriodicalIF":1.5,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/7a/59_1.PMC8791776.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AMP-activated Protein Kinase Activation Suppresses Protein Synthesis and mTORC1 Signaling in Chick Myotube Cultures.","authors":"Kazuki Nakashima,&nbsp;Aiko Ishida","doi":"10.2141/jpsa.0210021","DOIUrl":"https://doi.org/10.2141/jpsa.0210021","url":null,"abstract":"<p><p>Protein synthesis in skeletal muscle is considered one of the most energy-consuming cellular processes. AMP-activated protein kinase (AMPK) is a metabolic master switch that regulates glucose and lipid metabolism, and it is implicated in protein synthesis control in skeletal muscles. The mechanistic target of rapamycin complex 1 (mTORC1) is a central regulator of protein metabolism in cells. However, the effect of AMPK activation on protein synthesis and mTORC1 signaling in chicken skeletal muscle remains unclear. Therefore, in this study, we aimed to investigate the effect of 5-aminoimidazole-4-carboxamide-1-<i>β</i>-D-ribofuranoside (AICAR), an AMPK activator, on protein synthesis and mTORC1 signaling in chick myotube cultures. The incubation of chick myotubes with AICAR (1 mM) for 3 h led to a significant increase in AMPK (Thr172) phosphorylation. Nonetheless, protein synthesis, measured using the surface sensing of translation method, was significantly decreased by AICAR. In addition, the phosphorylation of p70 ribosomal S6 kinase 1 (S6K1, Thr389), S6 ribosomal protein (Ser240/244), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1, Thr37/46) was significantly reduced by AICAR. These results suggest that AMPK activation suppresses protein synthesis and mTORC1 signaling (through the phosphorylation of S6K1, S6 ribosomal protein, and 4E-BP1) in chick myotubes.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"81-85"},"PeriodicalIF":1.5,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/65/59_81.PMC8791771.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}