Journal of Poultry Science最新文献_第7页

Regulation of autophagy in chick myotube cultures: Effect of uncoupling mitochondrial oxidative phosphorylation. 鸡肌管细胞自噬的调节:线粒体氧化磷酸化的解偶联作用。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2023-01-01 DOI: 10.2141/jpsa.2023022

Kazuki Nakashima, Aiko Ishida

{"title":"Regulation of autophagy in chick myotube cultures: Effect of uncoupling mitochondrial oxidative phosphorylation.","authors":"Kazuki Nakashima, Aiko Ishida","doi":"10.2141/jpsa.2023022","DOIUrl":"https://doi.org/10.2141/jpsa.2023022","url":null,"abstract":"Abstracts: Skeletal muscles have a high demand for ATP, which is met largely through mitochondria oxidative phosphorylation. Autophagy is essential for the maintenance of skeletal muscle mass under catabolic conditions. This study investigated the effect of uncoupling mitochondrial oxidative phosphorylation on autophagy in chicken skeletal muscle. Chick myotubes were incubated with the mitochondrial uncoupler carbonyl cyanide m-chlorophenyl hydrazone (CCCP) at 25 μM for 3h. CCCP prevented the phosphorylation of p70 ribosomal S6 kinase 1 (Thr389), S6 ribosomal protein (Ser240/244), and eukaryotic translation initiation factor 4E-binding protein 1 (Thr37/46), which are the measures of the mechanistic target of rapamycin complex 1 (mTORC1) activity. CCCP significantly increased cytoplasmic and mitochondrial LC3-II content, which act as indices of index for autophagosome formation and mitophagy, respectively, but did not influence the expression of autophagy-related genes LC3B, GABARAPL1, and ATG12. Finally, surface sensing of translation method revealed that protein synthesis, a highly energy consuming process, was significantly decreased upon CCCP treatment. These results indicate that the uncoupling of mitochondrial oxidative phosphorylation stimulates autophagy and inhibits protein synthesis through mTORC1 signaling in chick myotube cultures.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"60 ","pages":"2023022"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1b/67/jpsa-60-2023022.PMC10410138.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10053900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of N^τ -Methylhistidine and N^π-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry. 液相色谱-串联质谱法定量测定鸡血浆中Nτ -甲基组氨酸和n π-甲基组氨酸。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2023-01-01 DOI: 10.2141/jpsa.2023017

Jun-Ichi Shiraishi, Daichi Ijiri, Ayumi Katafuchi, Shozo Tomonaga, Saki Shimamoto, Hanwool Do, Shinya Ishihara, Akira Ohtsuka

{"title":"Quantification of Nτ -Methylhistidine and Nπ-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry.","authors":"Jun-Ichi Shiraishi, Daichi Ijiri, Ayumi Katafuchi, Shozo Tomonaga, Saki Shimamoto, Hanwool Do, Shinya Ishihara, Akira Ohtsuka","doi":"10.2141/jpsa.2023017","DOIUrl":"https://doi.org/10.2141/jpsa.2023017","url":null,"abstract":"The concentration of Nτ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of Nτ-methylhistidine and its isomer Nπ-methylhistidine in chicken plasma, using liquid chromatography-tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56-50.00 μmol/L for Nτ-methylhistidine and 0.78-25.00 μmol/L for Nπ-methylhistidine. The proposed method detected changes in the plasma levels of Nτ-methylhistidine and Nπ-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of Nτ-methylhistidine and Nπ-methylhistidine in chicken plasma.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"60 ","pages":"2023017"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/35/e1/jpsa-60-2023017.PMC10357029.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9867138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Gluconeogenesis in the Yolk Sac Membrane: Enzyme Activity, Gene Expression, and Metabolites During Layer Chicken Development. 蛋鸡卵黄囊膜中的糖异生:酶活性、基因表达和代谢产物。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2023-01-01 DOI: 10.2141/jpsa.2023020

Mitsuhiro Shibata, Atsushi Iwasawa, Masato Yayota

{"title":"Gluconeogenesis in the Yolk Sac Membrane: Enzyme Activity, Gene Expression, and Metabolites During Layer Chicken Development.","authors":"Mitsuhiro Shibata, Atsushi Iwasawa, Masato Yayota","doi":"10.2141/jpsa.2023020","DOIUrl":"https://doi.org/10.2141/jpsa.2023020","url":null,"abstract":"ABSTRACT Yolk sac membranes of layer eggs were collected daily (n = 7–9) from day three of incubation to day three post-hatch, and mRNA expression and activities were quantified for key gluconeogenesis enzymes (glucose-6-phosphatase, fructose-1,6-bisphosphatase, cytosolic and mitochondrial phosphoenolpyruvate carboxykinases, and pyruvate carboxylase). Lactate, triglycerides, non-esterified fatty acids, glycogen, and glucose in the yolk sac membrane, and blood glucose levels were also measured. The mRNA expression and activity were detected for all enzymes. Differences in expression levels and enzyme activities seemed to reflect the embryo’s developmental environment and physiological demands at different developmental stages. During the first week to the mid-second week of incubation, the expression and activity of gluconeogenic enzymes and lactate concentrations were high, suggesting an active period of gluconeogenesis from lactate, reflecting possible hypoxia in the embryo before completed formation of the chorioallantoic capillaries. From the mid-second week to mid-third week, when embryos were in an aerobic state, the triglyceride and non-esterified fatty acid contents increased in the yolk sac. Triglycerides from yolk lipids are typically hydrolyzed to produce non-esterified fatty acids as an energy source, whereas the glycerol skeleton is used for gluconeogenesis. In the late third week, when embryos were considered to re-enter an anaerobic state, the mRNA expression and enzyme activity of only glucose-6-phosphatase were high and the amount of glycogen in the yolk sac was reduced. Therefore, it is suggested that gluconeogenesis activity is low during this period, and the carbohydrates stored in the yolk sac membrane are secreted into the blood as energy for hatching. This study confirmed the role of the yolk sac membrane as a vital gluconeogenic organ during chicken egg incubation.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"60 2","pages":"2023020"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/97/jpsa-60-2023020.PMC10406515.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9970815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the mtDNA D-loop Region Casts New Light on Philippine Red Junglefowl Phylogeny and Relationships to Other Junglefowl Species in Asia. 对mtDNA D环区的分析为菲律宾红丛林鸟的系统发育以及与亚洲其他丛林鸟物种的关系提供了新的线索。

IF 1.8 4区农林科学

Journal of Poultry Science Pub Date : 2022-10-25 DOI: 10.2141/jpsa.0210140

Jade Dhapnee Z Compendio, Jam Marrie Nanche P Mantana, Masahide Nishibori

{"title":"Analysis of the mtDNA D-loop Region Casts New Light on Philippine Red Junglefowl Phylogeny and Relationships to Other Junglefowl Species in Asia.","authors":"Jade Dhapnee Z Compendio, Jam Marrie Nanche P Mantana, Masahide Nishibori","doi":"10.2141/jpsa.0210140","DOIUrl":"10.2141/jpsa.0210140","url":null,"abstract":"Red junglefowl (RJF) is considered the ancestor of domestic chickens. However, the possible maternal origin, genetic diversity, and subspecies classification of the Philippine (PH) RJF remains uncertain. In this study, the complete mitochondrial DNA (mtDNA) D-loop sequence of 55 PH RJFs collected from the mountainous areas of Occidental Mindoro, Palawan, Agusan del Norte, Capiz, Leyte, Iloilo, and Guimaras were analyzed and compared with chicken reference sequences. Phylogenetic analysis revealed multiple maternal origins of the PH RJFs based on haplogroups D, E, and Y classification. This was supported by PH RJFs and RJFs from other Asian countries sharing a clade. A median-joining network also revealed the haplotype sharing of the PH RJFs and Indonesian RJF, demonstrating common maternal ancestry. High haplotype and nucleotide diversity were also observed at all sampling sites. Analysis of molecular variance indicated that the principal molecular variance existed within populations (81.23%) rather than among populations (18.77%). A population neutrality test and Bayesian skyline plot (BSP) analysis elucidated the RJF maternal effective population size expansion in the Philippines that possibly started approximately 2,800-3,000 years ago. The co-existence of Gallus gallus bankiva and Gallus gallus gallus in the Philippines was also verified. The haplotype sharing of the current RJF samples with commercial chickens suggested the need to formulate conservation programs that would protect the RJFs in the Philippines.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 4","pages":"305-315"},"PeriodicalIF":1.8,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9c/c2/59_305.PMC9596289.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40469049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of a Rearing Dietary Protein Regimen on Productive Performance, Egg Quality, and Bone Quality of Laying Hens. 饲粮蛋白质方案对蛋鸡生产性能、蛋品质和骨品质的影响。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-10-25 DOI: 10.2141/jpsa.0220042

Cecilia T Oluwabiyi, Jingpeng Zhao, Hongchao Jiao, Xiaojuan Wang, Haifang Li, Yunlei Zhou, Hai Lin

{"title":"Effects of a Rearing Dietary Protein Regimen on Productive Performance, Egg Quality, and Bone Quality of Laying Hens.","authors":"Cecilia T Oluwabiyi, Jingpeng Zhao, Hongchao Jiao, Xiaojuan Wang, Haifang Li, Yunlei Zhou, Hai Lin","doi":"10.2141/jpsa.0220042","DOIUrl":"https://doi.org/10.2141/jpsa.0220042","url":null,"abstract":"The pullet phase is an important stage in the development of laying hens when the development of organs, including reproductive organs and bones, is rapid. However, in recent years, few studies have focused on this crucial stage. The purpose of this study was to evaluate the effect of a dietary crude protein (CP) regimen during the rearing period (9-21 weeks (wks) of age) on pullet development and the subsequent performance, egg quality, and bone quality of Hy-Line Brown laying hens. A total of 256 pullets were randomly assigned to two treatments. Each treatment was replicated eight times with 16 pullets per replicate (n=8), which were fed ad libitum using either of the two CP regimens: (1) 14%-18% CP (fed with 14% and 18% CP from 9-17 wks and 18-21 wks, respectively); (2) 16% CP (fed with 16% CP from 9-21 wks of age). At 21 wks of age, eight birds per treatment were randomly selected to evaluate body composition and ovarian development. For quality analysis, eggs were collected at 28, 32, 36, and 70 wks. At 70 wks of age, eight hens per treatment were selected to evaluate bone quality. There were no treatment differences in pullet performance, body composition, and ovarian development at 21 wks. The dietary CP regimen during the rearing period (9-21 wks) did not influence laying performance during the laying period. There were no treatment differences in tibial and femoral quality at 70 wks. Egg quality results showed an inconsistent trend. It was concluded that the pullets fed with the low CP grower diet (14%) during the pullet period and a high CP pre-lay diet (18%) from 18-21 wks of age developed properly and had satisfactory laying performance. However, the rearing diet did not enhance bone quality.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 4","pages":"328-337"},"PeriodicalIF":1.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/51/59_328.PMC9596298.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40469046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Oxygen Gas Injection on the Subsequent Development of Chick Embryos in a Shell-Less Culture System. 注氧对无壳培养体系中鸡胚后续发育的影响。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-10-25 DOI: 10.2141/jpsa.0220031

Katsuya Obara, Chizuka Obara Henmi, Mitsuru Naito, Ikki Mitsui, Yumi Une, Atsushi Asano, Atsushi Tajima

{"title":"Effects of Oxygen Gas Injection on the Subsequent Development of Chick Embryos in a Shell-Less Culture System.","authors":"Katsuya Obara, Chizuka Obara Henmi, Mitsuru Naito, Ikki Mitsui, Yumi Une, Atsushi Asano, Atsushi Tajima","doi":"10.2141/jpsa.0220031","DOIUrl":"https://doi.org/10.2141/jpsa.0220031","url":null,"abstract":"The effects of oxygen gas injection starting on day 17 of incubation (D17) in a chick shell-less culture system (cSLC) on the subsequent embryo development were examined on day 19 of incubation (D19). On D19 of cSLC, the plasma phosphorus and total cholesterol concentrations of the embryos were significantly higher (P<0.05), while the plasma calcium concentrations were significantly lower (P<0.05) than those in the intact control (IC) group. However, no significant differences in embryo viability and other major blood component levels were observed among the experimental groups (P>0.05). The percutaneous oxygen saturation was lower in D17-cSLC embryos before oxygen gas supplementation than in the IC (P<0.05) embryos. Severe renal tubular degeneration of the metanephros was observed in D19-cSLC embryos despite oxygen gas injection starting from D17. These results indicate that D19-cSLC embryos are hypoxia even after injecting oxygen gas starting on D17.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 4","pages":"371-377"},"PeriodicalIF":1.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b3/f5/59_371.PMC9596291.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Connexin 43 is Localized in Gizzard Smooth Muscle Cells during Chicken Development. 连接蛋白43在鸡发育过程中定位于砂囊平滑肌细胞。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-10-25 DOI: 10.2141/jpsa.0220003

Kosuke Tokunaga, Shota Akimoto, Machiko Aiba, Mutsuki Nakagomi, Takahiro Suzuki, Ryuichi Tatsumi, Mako Nakamura

{"title":"Connexin 43 is Localized in Gizzard Smooth Muscle Cells during Chicken Development.","authors":"Kosuke Tokunaga, Shota Akimoto, Machiko Aiba, Mutsuki Nakagomi, Takahiro Suzuki, Ryuichi Tatsumi, Mako Nakamura","doi":"10.2141/jpsa.0220003","DOIUrl":"https://doi.org/10.2141/jpsa.0220003","url":null,"abstract":"Smooth muscle cells are widely distributed in the digestive organs of chickens. They exist as single cells, but adhere to each other to function synchronously. In this study, the expression of the gap junction protein connexin 43 (Cx43) in chicken gizzards was investigated at embryonic days (E) 10, E15, and E18. Gizzards have an abundance of smooth muscle cells because of their thick muscle layers, which enable easy analysis of the cells. Morphological observations and expression patterns of smooth muscle markers were confirmed. Next, we observed where the markers were localized in the gizzard tissue at E10, E15, and E18. Finally, the expression pattern of Cx43 in primary cultured smooth muscle cells from E15 gizzards was investigated. The analysis revealed the expression and localization of Cx43 and calponin 1 in the smooth muscle layers, and 3D analysis revealed dynamic changes in the localization pattern of Cx43 from E10 to E15. Cultured smooth muscle cells confirmed that Cx43 was expressed in the cell membrane and cytosol. In conclusion, Cx43 expression was identified in chicken gizzards at E10, E15, and E18, which was localized differently during development. The expression was broad at E10, and became restricted at E15 and E18. Primary culture of smooth muscle cells showed that Cx43 was present in the cell membrane and cytosol. This suggests that Cx43 is actively translated into the cytosol at E10, forming a hexamer, and shuttling the cell membrane to function as a gap junction.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 4","pages":"338-347"},"PeriodicalIF":1.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/88/1b/59_338.PMC9596294.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Conditioned Taste Aversion to L-Amino Acid Taste Stimuli and Oral Transcriptional Changes to Type 1 Taste Receptors T1R1 and T1R3 on Chronic Exposure to L-Alanine Solution in Chickens. 慢性暴露于l -丙氨酸溶液的鸡对l -氨基酸味觉刺激的条件性味觉厌恶及1型味觉受体T1R1和T1R3的口腔转录变化

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-10-25 DOI: 10.2141/jpsa.0210128

Yuta Yoshida, Ryota Tanaka, Shu Fujishiro, Shotaro Nishimura, Shoji Tabata, Fuminori Kawabata

{"title":"Conditioned Taste Aversion to L-Amino Acid Taste Stimuli and Oral Transcriptional Changes to Type 1 Taste Receptors T1R1 and T1R3 on Chronic Exposure to L-Alanine Solution in Chickens.","authors":"Yuta Yoshida, Ryota Tanaka, Shu Fujishiro, Shotaro Nishimura, Shoji Tabata, Fuminori Kawabata","doi":"10.2141/jpsa.0210128","DOIUrl":"https://doi.org/10.2141/jpsa.0210128","url":null,"abstract":"Elucidating taste sensing systems in chickens is an important step toward understanding poultry nutrition. Amino acid taste receptors, type 1 taste receptors 1 and 3 (T1R1 and T1R3, respectively), are expressed in chicken taste cells, and chicken T1R1/T1R3 is activated by L-alanine (L-Ala) and L-serine (L-Ser), but not by L-proline (L-Pro). However, it is not clear whether chickens have a gustatory perception of L-amino acids. Here, we found that chickens conditioned to avoid either L-Ala, L-Ser, or L-Pro solutions could successfully learn to avoid the corresponding L-amino acid solution in the conditioned taste aversion (CTA) test. Because CTA is a well-established learning paradigm generated specifically by pairing gustatory perception and gastrointestinal malaise, the present study suggests that chickens can sense L-amino acids by gustatory perception. In addition, we found that the expression of the T1R1 and T1R3 genes was significantly downregulated in response to chronic exposure to L-Ala solution, but not to acute oral stimulation. Taken together, the present study suggests that chickens have a gustatory perception of L-amino acids, and the expression of T1R1/T1R3 mRNAs in the oral cavity can be regulated by L-amino acid intake. Since chickens can detect L-Pro solutions, additional amino acid receptors, other than T1R1/T1R3, may be involved in L-amino acid taste detection in chickens.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 4","pages":"348-356"},"PeriodicalIF":1.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/5a/59_348.PMC9596290.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40469045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Regulation of Prolactin Release at the End Stage of Chicken Embryogenesis. 鸡胚发生末期催乳素释放的调控。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-10-25 DOI: 10.2141/jpsa.0220023

Norio Kansaku, Shin Wakui, Tomohiro Sasanami, Takeshi Ohkubo

{"title":"Regulation of Prolactin Release at the End Stage of Chicken Embryogenesis.","authors":"Norio Kansaku, Shin Wakui, Tomohiro Sasanami, Takeshi Ohkubo","doi":"10.2141/jpsa.0220023","DOIUrl":"https://doi.org/10.2141/jpsa.0220023","url":null,"abstract":"Difference of onset of increase of PRL content in the anterior pituitary gland and plasma PRL concentration during the late stage of chicken embryogenesis is well known. To investigate the disagreement, changes in PRL content and PRL mRNA levels, and the effects of vasoactive intestinal polypeptides (VIP) on PRL release and PRL mRNA expression were examined using western blot analysis and real-time PCR quantification. Changes in SPRL content were strongly correlated with PRL mRNA levels. The increase in PRL content on day 17 of incubation may be caused by the increase in PRL mRNA levels on day 16 of incubation. Additionally, the effects of VIP on PRL release from the embryonic anterior pituitary gland were not observed until day 18 of embryogenesis. These results suggest that increased levels of PRL mRNA and PRL content in the anterior pituitary gland are closely correlated. However, the increased expression of PRL mRNA observed on day 17 and the initiation of PRL release from the anterior pituitary gland on day 19 were differentially regulated. According to the results of western blot analysis, the proportion of glycosylated PRL (G-PRL) and non-glycosylated PRL (NG-PRL) in the anterior pituitary gland at the end stage of development differed from the proportion of PRL released from the anterior pituitary gland. According to the results of two-dimensional western blot analysis, no isoforms with different isoelectric points were detected in the culture medium on days 19 and 20. These data suggest that the peptide chains of G-PRL and NG-PRL were not modified. In conclusion, the differentiation of PRL-producing cells and the maturation of the hypothalamus and anterior pituitary gland were completed at the end stage of incubation, and that different factors regulated the initiation of PRL mRNA expression before day 18 of incubation.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 4","pages":"364-370"},"PeriodicalIF":1.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/71/3d/59_364.PMC9596296.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40469047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Repression Effects of Hydrolysates from Hen-Egg Proteins on Amyloid Fibril Formation. 鸡蛋蛋白水解物对淀粉样纤维形成的抑制作用。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-10-25 DOI: 10.2141/jpsa.0220038

Yukiko Muroi, Izumi Aburaya, Takuro Shima, Mitsuharu Matsumoto, Ryo Sasahara, Takahisa Suzuki, Keiichi Watanabe, Koji Wada, Yasushi Sugimoto

{"title":"Repression Effects of Hydrolysates from Hen-Egg Proteins on Amyloid Fibril Formation.","authors":"Yukiko Muroi, Izumi Aburaya, Takuro Shima, Mitsuharu Matsumoto, Ryo Sasahara, Takahisa Suzuki, Keiichi Watanabe, Koji Wada, Yasushi Sugimoto","doi":"10.2141/jpsa.0220038","DOIUrl":"https://doi.org/10.2141/jpsa.0220038","url":null,"abstract":"Amyloid fibrils, which are formed from aggregates of aberrant proteins, can cause various forms of amyloidosis (including Alzheimer's disease). Such disorders often occur in elderly populations and are suspected to be lifestyle related. Thus, it has been speculated that some foodstuffs could be beneficial for preventing amyloidosis. In this study, we determine whether fibril formation by the hen egg white lysozyme (HEWL) could be inhibited by conducting a thioflavin T assay followed by fluorescence and electron microscopy observations. The results demonstrated that four peptide specimens prepared by the hydrolysis of crude proteins from the egg white, egg yolk, chalazae, and eggshell membrane of hen eggs effectively inhibited HEWL fibril formation. Among the four specimens, peptides from chalazae exhibited the highest preventive ability. The superiority of chalaza peptides was also observed when fibril formation was assayed using a full-length human lysozyme and human amyloid β peptide 1-42, which is the key factor for the development of Alzheimer's disease. Our study of the fibrillization of the human lysozyme also showed that metal ions (Zn2+, Ca2+, Co2+, Mn2+ and Al3+) promoted fibrillization, and their effects were abolished by the peptide specimens (especially by chalaza peptides). Thus, we conclude that chicken-egg proteins could be a convenient source of therapeutic materials for amyloidosis.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 4","pages":"384-391"},"PeriodicalIF":1.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d5/72/59_384.PMC9596292.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0