Journal of Poultry Science最新文献_第10页

1,25-Dihydroxycholecalciferol Improved the Growth Performance and Upregulated the Calcium Transporter Gene Expression Levels in the Small Intestine of Broiler Chickens 1,25-二羟基胆骨化醇提高肉仔鸡生长性能，上调小肠钙转运蛋白基因表达水平

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210019

Lihua Wu, Xiaona Wang, X. Lv, Lei He, Hongxia Qu, C. Shi, Lia Zhang, Jinliang Zhang, Zhixiang Wang, Jincheng Han

{"title":"1,25-Dihydroxycholecalciferol Improved the Growth Performance and Upregulated the Calcium Transporter Gene Expression Levels in the Small Intestine of Broiler Chickens","authors":"Lihua Wu, Xiaona Wang, X. Lv, Lei He, Hongxia Qu, C. Shi, Lia Zhang, Jinliang Zhang, Zhixiang Wang, Jincheng Han","doi":"10.2141/jpsa.0210019","DOIUrl":"https://doi.org/10.2141/jpsa.0210019","url":null,"abstract":"1,25-Dihydroxycholecalciferol (1,25-(OH)2-D3) is the final active product of vitamin D. This study aimed to investigate the effects of 1,25-(OH)2-D3 on growth performance, bone development, and calcium (Ca) transporter gene expression levels in the small intestine of broiler chickens. On the day of hatching, 140 female Ross 308 broilers were randomly allotted into two treatments with five replicates (14 birds per replicate). Two levels of 1,25-(OH)2-D3 (0 and 1.25 µg/kg) were added to the basal diet without vitamin D. Results showed that the addition of 1.25 µg/kg 1,25-(OH)2-D3 increased the average daily feed intake and the average daily gain and decreased the feed conversion ratio and mortality in 1- to 19-day-old broiler chickens compared with the basal diet without vitamin D (P<0.05). 1,25-(OH)2-D3 also enhanced the length, weight, ash weight, and the percentage contents of ash, Ca, and P in the tibia and femur of broilers (P<0.05). The mRNA expression levels of the Ca-binding protein (CaBP-D28k) in the duodenum, jejunum, and ileum of 19-day-old broilers increased to 88.1-, 109.1-, and 2.7-fold, respectively, after adding 1,25-(OH)2-D3 (P<0.05). The mRNA expression levels of the plasma membrane Ca ATPase 1b (PMCAlb) in the duodenum and the sodium (Na)/ Ca exchanger 1 (NCX1) in the duodenum and the jejunum were also enhanced to 1.57-2.86 times with the addition of 1,25-(OH)2-D3 (P<0.05). In contrast, the mRNA expression levels of PMCA1b and NCX1 in the ileum and that of vitamin D receptor (VDR) in the small intestine were not affected by 1,25-(OH)2-D3 (P>0.05). These data indicate that 1,25-(OH)2-D3 upregulated Ca transporter gene transcription and promoted Ca2+ absorption in the small intestine, especially in the proximal intestine (duodenum and jejunum), thereby improving growth performance and bone mineralization in broiler chickens.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"129 - 136"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42289264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Calcium Lactate on the Development of Chicken Embryos in a Shell-less Culture System up to Day Seventeen of Incubation 乳酸钙对无壳培养鸡胚胎发育的影响

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210024

Katsuya Obara, Chizuka Obara (Henmi), M. Naito, I. Mitsui, Y. Une, A. Asano, A. Tajima

引用次数: 2

Inositol-1,4,5-Trisphosphate Receptor-1 and -3 and Ryanodine Receptor-3 May Increase Ooplasmic Ca2+ During Quail Egg Activation 肌醇-1,4,5-三磷酸受体-1和-3和赖诺定受体-3在鹌鹑卵活化过程中可能增加卵浆Ca2+

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210041

S. Mizushima, T. Sasanami, T. Ono, N. Kansaku, A. Kuroiwa

{"title":"Inositol-1,4,5-Trisphosphate Receptor-1 and -3 and Ryanodine Receptor-3 May Increase Ooplasmic Ca2+ During Quail Egg Activation","authors":"S. Mizushima, T. Sasanami, T. Ono, N. Kansaku, A. Kuroiwa","doi":"10.2141/jpsa.0210041","DOIUrl":"https://doi.org/10.2141/jpsa.0210041","url":null,"abstract":"We previously reported that egg activation in Japanese quail is driven by two distinct types of intracellular Ca2+ ([Ca2+]i): transient elevations in [Ca2+]i induced by phospholipase Czeta 1 (PLCZ1) and long-lasting spiral-like Ca2+ oscillations by citrate synthase (CS) and aconitate hydratase 2 (ACO2). Although the blockade of inositol 1,4,5-trisphosphate receptors (ITPRs) before microinjections of PLCZ1, CS, and ACO2 cRNAs only prevented transient increases in [Ca2+]i, a microinjection of an agonist of ryanodine receptors (RYRs) induced spiral-like Ca2+ oscillations, indicating the involvement of both ITPRs and RYRs in these events. In this study, we investigated the isoforms of ITPRs and RYRs responsible for the expression of the two types of [Ca2+]i increases. RT-PCR and western blot analyses revealed that ITPR1, ITPR3, and RYR3 were expressed in ovulated eggs. These proteins were degraded 3 h after the microinjection of PLCZ1, CS, and ACO2 cRNAs, which is the time at which egg activation was complete. However, degradation of ITPR1 and ITPR3, but not RYR3, was initiated 30 min after a single injection of PLCZ1 cRNA, corresponding to the time of the initial Ca2+ wave termination. In contrast, RYR3 degradation was observed 3 h after the microinjection of CS and ACO2 cRNAs. These results indicate that ITPRs and RYR3 differentially mediate in creases in [Ca2+]i during egg activation in Japanese quail, and that downregulation of ITPRs and RYR3-mediated events terminate the initial Ca2+ wave and spiral-like Ca2+ oscillations, respectively.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"175 - 181"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44041407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Relationship between Mucosal Barrier Function of the Oviduct and Intestine in the Productivity of Laying Hens 输卵管和肠道黏膜屏障功能与蛋鸡生产能力的关系

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210090

T. Nii

{"title":"Relationship between Mucosal Barrier Function of the Oviduct and Intestine in the Productivity of Laying Hens","authors":"T. Nii","doi":"10.2141/jpsa.0210090","DOIUrl":"https://doi.org/10.2141/jpsa.0210090","url":null,"abstract":"The mucosa of the intestine and oviduct of hens are susceptible to pathogens. Pathogenic infections in the mucosal tissues of laying hens lead to worsened health of the host animal, decreased egg production, and bacterial contamination of eggs. Therefore, better understanding of the mechanisms underlying mucosal barrier function is needed to prevent infection by pathogens. In addition, pathogen infection in the mucosal tissue generally causes mucosal inflammation. Recently, it has been shown that inflammation in the oviduct and intestinal tissue caused by disruption of the mucosal barrier function, can affect egg production. Therefore, it is vitla to understand the relationship between mucosal barrier function and egg production to improve poultry egg production. This paper reviews the studies on (1) oviductal mucosal immune function and egg production, (2) intestinal inflammation and egg production, and (3) improvement of mucosal immune function by probiotics. The findings introduced in this review will contribute to the understanding of the mucosal barrier function of the intestine and oviduct and improve poultry egg production in laying hens.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"105 - 113"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42182090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Isolation of Lactobacillaceae bacteria from feces of ostrich (Struthio camelus) 鸵鸟粪便中乳杆菌科细菌的分离

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210001

Misa Onodera, Tomomi Ban-Tokuda, H. Matsui

引用次数: 0

Role of Corticosterone in Lipid Metabolism in Broiler Chick White Adipose Tissue 皮质酮在肉仔鸡白色脂肪组织脂质代谢中的作用

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210060

K. Honda, Kiyotaka Kurachi, Shoko Takagi, Takaoki Saneyasu, H. Kamisoyama

{"title":"Role of Corticosterone in Lipid Metabolism in Broiler Chick White Adipose Tissue","authors":"K. Honda, Kiyotaka Kurachi, Shoko Takagi, Takaoki Saneyasu, H. Kamisoyama","doi":"10.2141/jpsa.0210060","DOIUrl":"https://doi.org/10.2141/jpsa.0210060","url":null,"abstract":"Excessive accumulation of body fat in broiler chickens has become a serious problem in the poultry industry. However, the molecular mechanism of triglyceride accumulation in chicken white adipose tissue (WAT) has not been elucidated. In the present study, we investigated the physiological importance of the catabolic hormone corticosterone, the major glucocorticoid in chickens, in the regulation of chicken WAT lipid metabolism. We first examined the effects of fasting on the mRNA levels of lipid metabolism-related genes associated with WAT, plasma corticosterone, and non-esterified fatty acid (NEFA). We then examined the effects of corticosterone on the expression of these genes in vivo and in vitro. In 10-day-old chicks, 3 h of fasting significantly decreased mRNA levels of lipoprotein lipase (LPL) in WAT and significantly elevated plasma concentrations of NEFA. Six hours of fasting significantly increased mRNA levels of adipose triglyceride lipase (ATGL) in WAT and significantly elevated plasma concentrations of corticosterone. On the other hand, fasting significantly reduced mRNA levels of LPL in WAT and elevated plasma concentrations of NEFA in 29-day-old chicks without affecting mRNA levels of ATGL in WAT or plasma corticosterone concentrations. Oral administration of corticosterone significantly reduced mRNA levels of LPL and significantly increased the mRNA levels of ATGL in WAT in 29-day-old chicks without affecting plasma NEFA concentrations. The addition of corticosterone to primary chicken adipocytes significantly increased mRNA levels of ATGL, whereas mRNA levels of LPL tended to decrease. NEFA concentrations in the culture medium were not influenced by corticosterone levels. These results suggest that plasma corticosterone partly regulates the gene expression of lipid metabolism-related genes in chicken WAT and this regulation is different from the acute elevation of plasma NEFA due to short-term fasting.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"152 - 158"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41511183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method 用crisi - pitch方法将一种荧光蛋白基因敲入鸡原始生殖细胞的Vasa同源位点

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210067

Ryo Ezaki, Kennosuke Ichikawa, M. Matsuzaki, H. Horiuchi

{"title":"Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method","authors":"Ryo Ezaki, Kennosuke Ichikawa, M. Matsuzaki, H. Horiuchi","doi":"10.2141/jpsa.0210067","DOIUrl":"https://doi.org/10.2141/jpsa.0210067","url":null,"abstract":"In chickens, primordial germ cells (PGCs) are effective targets for advanced genome editing, including gene knock-in. Although a long-term culture system has been established for chicken PGCs, it is necessary to select a gene-editing tool that is efficient and precise for editing the PGC genome while maintaining its ability to contribute to the reproductive system. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) and CRISPR-mediated precise integration into the target chromosome (CRIS-PITCh) methods are superior as the donor vector is easier to construct, has high genome editing efficiency, and does not select target cells, compared to the homologous recombination method, which has been conventionally used to generate knock-in chickens. In this study, we engineered knock-in chicken PGCs by integrating a fluorescent protein gene cassette as a fusion protein into the chicken vasa homolog (CVH) locus of chicken PGCs using the CRIS-PITCh method. The knock-in PGCs expressed the fluorescent protein in vitro and in vivo, facilitating the tracking of PGCs. Furthermore, we characterized the efficiency of engineering double knock-in cell lines. Knock-in cell clones were obtained by limiting dilution, and the efficiency of engineering double knock-in cell lines was confirmed by genotyping. We found that 82% of the analyzed clones were successfully knocked-in into both alleles. We suggest that the production of model chicken from the knock-in PGCs can contribute to various studies, such as the elucidation of the fate of germ cells and sex determination in chicken.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"182 - 190"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45404785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of Estradiol on Expression of Estrogen Receptor and Collagen mRNAs in Chick Skin 雌二醇对鸡皮肤雌激素受体和胶原蛋白mrna表达的影响

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-04-25 DOI: 10.2141/jpsa.0210093

S. Nishimura, Sayaka Arai, Mizuki Ohtani, Yuri Shimomura, S. Tabata

{"title":"Effects of Estradiol on Expression of Estrogen Receptor and Collagen mRNAs in Chick Skin","authors":"S. Nishimura, Sayaka Arai, Mizuki Ohtani, Yuri Shimomura, S. Tabata","doi":"10.2141/jpsa.0210093","DOIUrl":"https://doi.org/10.2141/jpsa.0210093","url":null,"abstract":"Skin thickness and strength differ between male and female chickens. This study aimed to clarify the effects of estradiol on the expression of estrogen receptors and collagen mRNA in chicken skin. Estradiol was administered to male chicks for 3 weeks, then cryosections of skin collected from the cervical, thoracic, dorsal, and pelvic limb regions were stained with hematoxylin and eosin, and dermal thickness was measured. Estrogen receptor and collagen mRNA expression was assessed using real-time RT-PCR, and collagen contents were determined. Estradiol did not alter dermal thickness or the collagen content of the skin from any tested region. Among the estrogen receptors, significantly more ESR1 mRNA was expressed in the thoracic skin of chicks administered with estradiol compared with vehicle (control), and in the thoracic skin compared with skin from other regions within each group. Estradiol did not affect ESR2, GPER, and COL1A1 mRNA expression. These results suggested that estradiol stimulates ESR1 expression in thoracic skin, but does not affect collagen synthesis in skin from any other region of male chicks.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"162 - 167"},"PeriodicalIF":1.5,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41917501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Detoxified Nano Sulfur Supplementation on the Growth, Nutrient Digestibility, Meat Quality, Excreta Microbes, Gas Emissions, and Blood Profiles of Broilers. 添加解毒纳米硫对肉鸡生长、营养物质消化率、肉质、排泄物微生物、气体排放和血液特征的影响

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0200111

Md Raihanul Hoque, Insun Park, Seyoung Mun, In Ho Kim

{"title":"Effect of Detoxified Nano Sulfur Supplementation on the Growth, Nutrient Digestibility, Meat Quality, Excreta Microbes, Gas Emissions, and Blood Profiles of Broilers.","authors":"Md Raihanul Hoque, Insun Park, Seyoung Mun, In Ho Kim","doi":"10.2141/jpsa.0200111","DOIUrl":"https://doi.org/10.2141/jpsa.0200111","url":null,"abstract":"A 35-day experiment was conducted to evaluate the effects of the supplementation of mineral detoxified sulfur dispersion ((DSD); Patent No.: 10-1997773) on the growth performance, meat quality, excreta microbiota, gas emissions, nutrient digestibility, and blood profiles of broilers. In total, 720 one-day-old ROSS 308 broilers, with an initial body weight of 41.9±0.8 g, were divided into two (2) treatment groups with 20 replicate pens/groups composed of 18 birds per pen. Treatments consisted of 1) CON (the control), normal drinking water and 2) TRT (the treatment group), CON+0.001% DSD (1000:1 dilution ratio). Average daily feed intake (ADFI) and feed conversion ratio (FCR) increased in the TRT group (P<0.05) between days 1 to 7 and days 7 to 21 of the experimental period. Similarly, body weight gain (BWG) showed a significant increase (P<0.05) in the DSD-supplemented group throughout in the length of the experiment. With regard to meat quality, redness (a*) was higher, while drip loss was lower, on the 7th day in the DSD group. Furthermore, DSD supplementation increased (P<0.05) Lactobacillus excreta but decreased E. coli concentrations in the TRT group compared to the CON group. Notably, nutrient digestibility, excreta gas emission, and blood profiles did not show any significant differences (P>0.05). DSD supplementation, administered through drinking water, has a positive impact on the growth performance, meat quality, and excreta microbiota of broiler chickens.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"48-55"},"PeriodicalIF":1.5,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/1e/59_48.PMC8791777.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic Analysis of Growth and Morphological Traits in Miniature Breeds of Japanese Indigenous Chickens. 日本地方鸡微型品种生长和形态性状的表型分析。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0200110

Ryoko Ono, Haruka Miyachi, Harui Usui, Rina Oguchi, Kenji Nishimura, Kanna Takahashi, Yuri Yamagishi, Nonoka Kawamura, Remi Matsushita, Tatsuhiko Goto

{"title":"Phenotypic Analysis of Growth and Morphological Traits in Miniature Breeds of Japanese Indigenous Chickens.","authors":"Ryoko Ono, Haruka Miyachi, Harui Usui, Rina Oguchi, Kenji Nishimura, Kanna Takahashi, Yuri Yamagishi, Nonoka Kawamura, Remi Matsushita, Tatsuhiko Goto","doi":"10.2141/jpsa.0200110","DOIUrl":"https://doi.org/10.2141/jpsa.0200110","url":null,"abstract":"Japanese indigenous chickens include approximately 50 breeds exhibiting various morphological traits, such as a long tail. These genetic resources will be important for revealing the genetic basis of morphological traits in the future. However, little is known about the phenotypic characteristics of each breed during the growth stages. To understand age-dependent changes in growth and morphological traits, we investigated tail length, tail number, body weight, and shank length at several time points using three genetically distinct Japanese indigenous chicken breeds. A total of 155 birds from the Tosa-jidori, Chabo, and Minohikichabo breeds were used for trait measurements from 1 to 36 weeks of age to reveal breed and sex effects. Significant sex differences through the growth stages were observed for all traits except for tail number. Although there were no clear breed differences in tail length traits at the 6- and 20-week stages, Minohikichabo ultimately had a significantly longer tail due to extended tail feather growth at later stages (28 and 36 weeks). By measuring two tail length variables (central and maximum), it was revealed that the shape of the tail feathers varies with the growth stage. Minohikichabo's tail number was higher than that of Tosajidori and Chabo at earlier ages (8 and 16 weeks), which leads to an elegant visual in Minohikichabo. Tosa-jidori's body weight was higher than that of Chabo and Minohikichabo, whereas the shank lengths of Chabo and Minohikichabo were shorter than those of Tosa-jidori. These differences in body weight and shank length were consistent from the early to late growth stages. These results revealed the age-dependency of growth and morphological trait breed characteristics.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"59 1","pages":"38-47"},"PeriodicalIF":1.5,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1a/cf/59_38.PMC8791770.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5