Quantification of N^τ -Methylhistidine and N^π-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry.

IF 1.8 4区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Poultry Science Pub Date : 2023-01-01 DOI:10.2141/jpsa.2023017

Jun-Ichi Shiraishi, Daichi Ijiri, Ayumi Katafuchi, Shozo Tomonaga, Saki Shimamoto, Hanwool Do, Shinya Ishihara, Akira Ohtsuka

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引用次数: 1

Abstract

The concentration of N^τ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N^τ-methylhistidine and its isomer N^π-methylhistidine in chicken plasma, using liquid chromatography-tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56-50.00 μmol/L for N^τ-methylhistidine and 0.78-25.00 μmol/L for N^π-methylhistidine. The proposed method detected changes in the plasma levels of N^τ-methylhistidine and N^π-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of N^τ-methylhistidine and N^π-methylhistidine in chicken plasma.

Abstract Image

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液相色谱-串联质谱法定量测定鸡血浆中Nτ -甲基组氨酸和n π-甲基组氨酸。

血浆中n τ-甲基组氨酸的浓度可作为骨骼肌蛋白分解的指标。本研究旨在建立鸡血浆中n π-甲基组氨酸及其异构体n π-甲基组氨酸浓度的定量测定方法，采用稳定同位素稀释液相色谱-串联质谱法。n τ-甲基组氨酸的可接受线性检测范围为1.56 ~ 50.00 μmol/L, n π-甲基组氨酸的可接受线性检测范围为0.78 ~ 25.00 μmol/L。该方法检测了空腹和复食后血浆中n τ-甲基组氨酸和n π-甲基组氨酸水平的变化。结果表明，该方法可用于鸡血浆中n τ-甲基组氨酸和n π-甲基组氨酸的同时测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

2.80

自引率

13.30%

发文量

审稿时长

12 months

期刊介绍： The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.