Journal of Plant Biochemistry and Biotechnology最新文献

{"title":"Mapping the spatiotemporal distributions of terpenoids and coumarins in Ferula ferulaeoides stem by using MALDI-MSI","authors":"Xuerui Zhang, FangWei Diao, Rong Guo, Zibo Wei, Kai Liao, Yun Zhu","doi":"10.1007/s13562-025-01018-y","DOIUrl":"https://doi.org/10.1007/s13562-025-01018-y","url":null,"abstract":"","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylic acid-triggered flowering in Lemna gibba: evidence of a stress-mediated pathway via NO and ROS","authors":"Samvedana Chauhan, Deepshikha Chatterjee, Latif Ahmad Peer, Bilal Ahmad Mir, Shashi B. Babbar","doi":"10.1007/s13562-025-01004-4","DOIUrl":"https://doi.org/10.1007/s13562-025-01004-4","url":null,"abstract":"","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"34 4","pages":"994-1009"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of SCAR marker for authentication in germplasm conservation and SSR & inter-transcribed spacer genetic diversity analysis in medicinally important herb Uraria picta.","authors":"Raja Feroz Ahmad Haji, Bashir Ahmad Dar, Sumit G. Gandhi, Irshad Ahmad Rather, Vijeshwar Verma","doi":"10.1007/s13562-024-00952-7","DOIUrl":"https://doi.org/10.1007/s13562-024-00952-7","url":null,"abstract":"","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"34 2","pages":"587-595"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in genome editing in plants within an evolving regulatory landscape, with a focus on its application in wheat breeding.","authors":"Natasha Brock, Navneet Kaur, Nigel G Halford","doi":"10.1007/s13562-025-00981-w","DOIUrl":"https://doi.org/10.1007/s13562-025-00981-w","url":null,"abstract":"<p><p>Population growth, diminishing resources and climate change are some of the many challenges that agriculture must address to satisfy the needs of the global population whilst ensuring the safety and nutritional value of our food. Wheat (<i>Triticum aestivum</i>) is tremendously important for human nutrition, providing starch (and, therefore, energy), fibre, protein, vitamins, and micronutrients. It is the second most widely grown crop behind maize (<i>Zea mays</i>), with 808 million tonnes of grain being produced in 2021-2022. In comparison, the production figure for 1961 was 222 million tonnes, and there have been similar increases for maize and rice (<i>Oryza sativa</i>). World population over the same period has increased from just over 3 billion to just over 8 billion, a stark reminder of just how important increased crop production has been in maintaining food security over that period, and for these cereals it has been achieved without additional land use. Plant breeding has played an important part in enabling crop production to keep increasing to meet demand and this will have to continue through the coming decades. Innovative technologies will play a part in that, and here we review how the new technology of genome editing is being applied in crop genetic improvement, with a focus on wheat. We cover oligonucleotide-directed mutagenesis and the use of site-directed nucleases, including meganucleases (MegNs), zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) nucleases. We describe established genome editing strategies, mainly involving gene 'knockouts', and the new applications of base and prime editing using CRISPR/Cas. We also discuss how genome editing for crop improvement is developing in the context of an evolving regulatory landscape.</p>","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"34 3","pages":"599-614"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144958434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creating a novel engineered tobacco chassis with downregulated expression of β-1,2-xylosyltransferase","authors":"Rong Han, Anran Xu, Yangzhong Wang, Jing Cao, Muhammad Naeem, Weihua Zhao, Lingxia Zhao","doi":"10.1007/s13562-024-00939-4","DOIUrl":"https://doi.org/10.1007/s13562-024-00939-4","url":null,"abstract":"","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"34 2","pages":"572-580"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequencing of Mesorhizobium Spp. NI-7, an efficient nitrogen-fixing microsymbiont of chickpea with potential to unravel the molecular mechanisms of symbiotic nitrogen fixation in legumes","authors":"Deevita Srivastava, Asim K Ghosh, Aashish Ranjan, Senjuti Sinharoy","doi":"10.1007/s13562-024-00917-w","DOIUrl":"https://doi.org/10.1007/s13562-024-00917-w","url":null,"abstract":"<p>Root nodule symbiosis is a major pillar of sustainable agriculture. The newly formed symbiotic organ in the legume root harbours rhizobacteria, which can fix atmospheric nitrogen into a bioavailable and reduced form, ammonia. Previously, we reported the isolation of an efficient <i>Mesorhizobium spp.</i> NI-7, from the interior of chickpea nodules. Here, we report the draft genome sequence of the <i>Mesorhizobium spp.</i> NI-7 and the comparative genomics among different Mesorhizobium strains that have adopted symbiosis during chickpea domestication. The draft genome of <i>Mesorhizobium spp.</i> NI-7 consists of a single 4.28 Mbp chromosome and a 359 Kbp plasmid. The 16 S rDNA sequence based phylogenetic analysis highlighted that <i>Mesorhizobium spp.</i> NI-7 belongs to a diverse Mesorhizobium clade that evolved during the domestication of chickpea. Comparative genomics among several Mesorhizobium strains identified 2193 common orthologous groups and several unique orthologous groups among the different Mesorhizobium pairs. The draft genome contains the essential nitrogen fixation genes along with the genes required for the nutrient exchange from the plant to the symbiont. Additionally, part of the symbiotic NOD-factor operon and Type III secretion system were also detected in the <i>Mesorhizobium spp.</i> NI-7 draft genome. The comparative genomics among the Mesorhizobium strains identified a subset of rhizobial genes that would have evolved during chickpea-Mesorhizobium adaptation to the Indian sub-continent. These genes are unique targets that can be validated in the future to understand the chickpea and Mesorhizobium adaptation. In summary, the draft genome sequencing of <i>Mesorhizobium spp.</i> NI-7 will equip the plant-microbe community with a chickpea-compatible Mesorhizobium strain isolated from India, suitable for both fundamental and advanced research on nodulation in chickpea, as well as for promoting sustainable agriculture in a comprehensive manner.</p>","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"63 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of functional microRNAs and their significant targets in maize plants","authors":"Abdul Baqi, Samiullah, Ghulam Mustafa Khan, Asadullah, Naqeebullah Khan, Attiq-Ur-Rehman, Alia Ahmed","doi":"10.1007/s13562-024-00918-9","DOIUrl":"https://doi.org/10.1007/s13562-024-00918-9","url":null,"abstract":"<p>Various metabolic and cell signaling processes influence the function of maize plant cells. miRNAs play numerous regulatory roles in regulating yield and protecting against various stressors. This study aims to identify and partially characterize some novel miRNAs in maize using in silico tools and provide a preliminary evaluation of their role. In this research, 20 novel conserved maize miRNAs belonging to 20 miRNA families were predicted using in silico tools and validated through RT-PCR. Consequently, 5850 different protein targets of these newly predicted miRNAs were identified via the psRNA Target approach. These targets included 20 significant ones involved in regulating metabolism, structural proteins, cell signaling proteins, and transportation factors. Moreover, the miRNA zma-miR5068 was predicted to be involved in the ubiquitin fusion protein process. Overall, this study examines novel maize miRNAs targeting several significant genes that could help manage the environment for better maize tolerance.</p>","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"135 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytosynthesis of copper nanoparticles using curcuma aromatica and its application in medical fabrics for antibacterial activity","authors":"Vardhana Janakiraman, Reethiga, B. Sampathkumar, N. K. Udaya Prakash","doi":"10.1007/s13562-024-00919-8","DOIUrl":"https://doi.org/10.1007/s13562-024-00919-8","url":null,"abstract":"<p>Antimicrobial textiles are crucial in various industries for combating contamination and improving hygiene. Copper's potent antimicrobial properties enhance infection control in medical fabrics. This study aims to synthesize copper in the form of nanoparticles CuNPs using <i>Curcuma aromatica</i>, to provide an effective, eco-friendly solution for antimicrobial medical textiles. The powder of the rhizome of <i>Curcuma aromatica</i> was boiled in sterile distilled water and the extract was used in the synthesis of CuNPs. The resulting CuNPs were characterized by UV–Vis spectroscopy, SEM, FTIR analysis, and XRD. The antibacterial efficacy of the synthesized CuNPs was tested against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> using the well diffusion method. The Cotton fabrics was embedded with synthesized CuNPs and again evaluated for its anti-bacterial property. The UV–VIS Spectroscopy showed absorption peaks above 554 nm due to the plasmon resonance of CuNPs. The size range of CuNPs was from 54 to 69 nm which was confirmed by SEM. FTIR analysis indicated the presence of various organic molecules associated with CuNPs. XRD analysis revealed the face-centered cubic crystal structure of the CuNPs. The CuNPs and CuNPs embedded cotton fabrics exhibited significant antibacterial activity against both <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. These findings support the use of <i>Curcuma aromatica</i> as a suitable source for CuNP synthesis and emphasize the significance of CuNPs in the development of effective antimicrobial fabrics for medical applications.</p>","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"15 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142227722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of free amino acids at metabolite and gene expression levels in Triticeae during cold acclimation","authors":"Kalpita Singh, Zsolt Gulyás, Benedikt Athmer, Bettina Kovács, Zsuzsanna Mednyánszky, Gábor Galiba, Nils Stein, Lívia Simon-Sarkadi, Gábor Kocsy","doi":"10.1007/s13562-024-00912-1","DOIUrl":"https://doi.org/10.1007/s13562-024-00912-1","url":null,"abstract":"<p>This study investigates the freezing tolerance and cold-induced changes in amino acid metabolism in three <i>Triticeae</i> species (rye, wheat, and barley) with varying levels of freezing tolerance. Freezing tests confirmed that rye exhibited the highest tolerance, while barley showed the highest sensitivity. Cold acclimation significantly increased total free amino acid levels, with wheat and barley showing nearly twice the accumulation compared to rye. The glutamate family of amino acids, particularly proline (Pro), γ-aminobutyric acid (GABA), and glutamine (Gln), displayed substantial increase during cold treatment. Pro levels were notably higher in freezing-tolerant wheat and barley genotypes, suggesting its role in osmotic stress mitigation. However, this correlation was absent in rye. Gene expression analysis revealed that cold-induced proline accumulation is likely regulated at the post-transcriptional level, particularly involving the <i>P5CS</i> gene. These findings highlight the species-specific metabolic adjustments and regulatory mechanisms underlying freezing tolerance in <i>Triticeae</i> species, emphasizing the central role of proline and glutamate family amino acids in cold acclimation.</p>","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142226516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-physiological and molecular responses of an ecologically important mangrove Kandelia candel (L.) Druce under salinity stress","authors":"Malini Bhattacharyya, Suraj Prasannakumari Meera, Ashifa Nizam, Ajay Kumar","doi":"10.1007/s13562-024-00904-1","DOIUrl":"https://doi.org/10.1007/s13562-024-00904-1","url":null,"abstract":"<p>Mangroves are ecologically important model plants for understanding their tolerance mechanisms to stresses. This research investigates the growth, survival performance, photosynthetic functioning, physiological, and molecular responses of <i>Kandelia candel</i> (L.) Druce under varying salinity regimes (10, 20, 30, 40, and 50 PPT NaCl) at two time points (15 and 24 DAT). The salinity-induced growth variations indicate early responses in seedlings<i>,</i> which begin at 10–20 PPT. Most leaf and stem-specific morphological parameters showed a decreasing trend with increasing salinity, leading to leaf shedding beyond 20 PPT. On the other hand, the root system showed better response, and the root-specific parameters increased up to 10–20 PPT. The salinity tolerance level of <i>K. candel</i> hardly reaches the seawater salinity range (~ 35 PPT). Beyond this, salinity hampers growth, ultimately leading to plant mortality at 50 PPT. The concentration of the photosystem pigments was higher in freshwater-grown plants, emphasising the facultative halophytic nature of <i>K</i>. <i>candel</i>. The photosynthetic performance completely ceased, initiating the progressive death of the above-ground plant tissues at 20 PPT, leading to root hydraulic failure and plant death at higher salinities. This study further explores the expression of key genes crucial for proper photosynthesis and water uptake. The RT-qPCR results show that the genes encoding photosystem proteins (<i>PsaA</i>, <i>PsaB</i>, and <i>PsbA</i>) and membrane transporters (<i>AKT1</i>, <i>NHX</i>, <i>NIP,</i> and <i>TIP</i>) are downregulated. In contrast, other photosynthetic genes (<i>RbcS</i>, <i>RbcL</i>, <i>Ycf3,</i> and <i>LHCB</i>) are upregulated under salinity stress. These results provide an understanding of the physiological and molecular basis of mangrove ecological responses to salinity stress.</p>","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"10 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142207705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}