Kalpita Singh, Zsolt Gulyás, Benedikt Athmer, Bettina Kovács, Zsuzsanna Mednyánszky, Gábor Galiba, Nils Stein, Lívia Simon-Sarkadi, Gábor Kocsy
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引用次数: 0
摘要
本研究调查了三种耐寒性不同的 Triticeae 植物(黑麦、小麦和大麦)的耐寒性以及寒冷引起的氨基酸代谢变化。冷冻试验证实,黑麦表现出最高的耐受性,而大麦则表现出最高的敏感性。冷适应显著增加了游离氨基酸总量,小麦和大麦的积累量几乎是黑麦的两倍。谷氨酸类氨基酸,特别是脯氨酸(Pro)、γ-氨基丁酸(GABA)和谷氨酰胺(Gln),在冷处理过程中含量大幅增加。耐寒小麦和大麦基因型中的 Pro 含量明显较高,这表明它在缓解渗透胁迫方面发挥了作用。然而,黑麦中却没有这种相关性。基因表达分析表明,冷诱导的脯氨酸积累可能是在转录后水平上调节的,特别是涉及到 P5CS 基因。这些发现突显了三尖杉科植物耐寒性背后的物种特异性代谢调整和调控机制,强调了脯氨酸和谷氨酸家族氨基酸在冷适应中的核心作用。
Comparative study of free amino acids at metabolite and gene expression levels in Triticeae during cold acclimation
This study investigates the freezing tolerance and cold-induced changes in amino acid metabolism in three Triticeae species (rye, wheat, and barley) with varying levels of freezing tolerance. Freezing tests confirmed that rye exhibited the highest tolerance, while barley showed the highest sensitivity. Cold acclimation significantly increased total free amino acid levels, with wheat and barley showing nearly twice the accumulation compared to rye. The glutamate family of amino acids, particularly proline (Pro), γ-aminobutyric acid (GABA), and glutamine (Gln), displayed substantial increase during cold treatment. Pro levels were notably higher in freezing-tolerant wheat and barley genotypes, suggesting its role in osmotic stress mitigation. However, this correlation was absent in rye. Gene expression analysis revealed that cold-induced proline accumulation is likely regulated at the post-transcriptional level, particularly involving the P5CS gene. These findings highlight the species-specific metabolic adjustments and regulatory mechanisms underlying freezing tolerance in Triticeae species, emphasizing the central role of proline and glutamate family amino acids in cold acclimation.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.