替代转录组与植物恢复能力:技术创新与生物学见解

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Ashish Kumar Pathak, Raja Jeet, Mathilde Moens, Yogesh Gupta, Vani Sharma, Kaushal Kumar Bhati
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引用次数: 0

摘要

先进的高通量测序方法的发展前所未有地揭示了与中心基因教条相关的生物分子多样性。大基因组学数据凸显了细胞机制的遗传调控和对环境刺激的生理反应所隐藏的复杂性。调查和鉴定替代 mRNA 形式和蛋白质多样性作为对环境刺激的适应机制,就是植物细胞中无与伦比的遗传复杂性的一个实例。在转录过程中和转录后,替代剪接和选择替代起始和终止位点会导致蛋白质家族中出现条件变体。许多此类蛋白质的生物学重要性已广为人知,尤其是在植物胁迫反应和发育的重编程过程中。有趣的是,在动物和模式植物的基因组和替代转录组测序方面的宝贵方法和技术成果正在为农作物的类似研究奠定基础。然而,鉴定替代转录组仍然是高等植物面临的一大挑战。因此,需要改进文库制备方法和数据分析管道。我们试图考察植物生理调控对环境适应的替代转录组相关研究的现状。此外,我们还评估了研究植物替代转录本组的最新技术进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Alternative transcriptomes and plant resilience: technological innovations and biological insights

Alternative transcriptomes and plant resilience: technological innovations and biological insights

The development of advanced high-throughput sequencing approaches has revealed the biomolecular diversity associated with central genetic dogmas like never before. Big genomics data highlight the hidden complexity of the genetic regulation of cellular machinery and physiological responses to environmental stimuli. The investigation and identification of alternative mRNA forms and protein diversity as adaptation mechanisms to environmental stimuli is one such case of unparallel genetic complexity in plant cells. Alternative splicing and selection of alternative start and stop sites during and after transcription lead to conditional variants across protein families. The biological importance of many such proteins is well understood, especially during reprogramming of plant stress responses and development. Interestingly, valuable methodologies and technical leads in the genome and alternative transcriptome sequencing from animals and model plants are now laying the groundwork for similar studies in crops. However, identifying alternative transcriptomes remains a major challenge for higher plants. Therefore, there is a need for improved library preparation methods and data analysis pipelines. We sought to examine the status of alternative transcriptome-associated studies on plant physiological regulation in response to environmental adaptation. In addition, we evaluated the recent technological advances available for studying alternative transcriptomes in plants.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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