Journal of Pathogens最新文献_第6页

Risk of Shiga Toxigenic Escherichia coli O157:H7 Infection from Raw and Fermented Milk in Sokoto Metropolis, Nigeria. 尼日利亚索科托大都市产志贺毒素大肠杆菌O157:H7感染生乳和发酵乳的风险

IF 2.6

Journal of Pathogens Pub Date : 2018-05-15 eCollection Date: 2018-01-01 DOI: 10.1155/2018/8938597

Yusuf Yakubu, Abdulmalik Bello Shuaibu, Aliyu Musawa Ibrahim, Ummukulthum Lawal Hassan, Raymond Junior Nwachukwu

{"title":"Risk of Shiga Toxigenic Escherichia coli O157:H7 Infection from Raw and Fermented Milk in Sokoto Metropolis, Nigeria.","authors":"Yusuf Yakubu, Abdulmalik Bello Shuaibu, Aliyu Musawa Ibrahim, Ummukulthum Lawal Hassan, Raymond Junior Nwachukwu","doi":"10.1155/2018/8938597","DOIUrl":"https://doi.org/10.1155/2018/8938597","url":null,"abstract":"Escherichia coli O157:H7 is an enteric foodborne pathogen associated with life threatening disease conditions. The enterobacteria are frequently found in cattle gastrointestinal tract with high potential of contaminating animal products such as meat, milk, and cheese. A cross-sectional study was conducted to investigate the presence of Shiga toxin-producing Escherichia coli O157:H7 in milk products sold within Sokoto metropolis. Two hundred and sixty (260) samples (comprising 160 raw and 100 fermented milk samples) were collected from different sources within the study area. Bacteriological isolation and biochemical characterization yielded Escherichia coli with a detection rate of 9.23% (24/260). Molecular identification of the recovered isolates by PCR amplification of the Stx1 gene revealed Escherichia coli O157:H7 with a positive rate of 20.83% (5/24). The overall prevalence of E. coli O157:H7 was 1.92% (5/260) and the positive proportions for raw and fermented milk samples were 1.86% (3/160) and 2.0% (2/100), respectively. Fisher's Exact test showed a nonsignificant association between the isolates and the different milk types (p = 0.943; OR = 0.94; [95% CI: 0.154-5.704]). The results revealed presence of Escherichia coli O157:H7 in raw and fermented milk sold within Sokoto metropolis, Nigeria. The findings indicate possible feacal contamination of the milk products, with serious public health consequences. This necessitates the need to screen other milk products produced in the area such as butter and cheese. Health authorities in the State need to enlighten dairy farmers on the zoonotic potential of Escherichia coli O157:H7 and the role of cattle in the spread of the pathogen.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/8938597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36188749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

QSAR Modeling and Molecular Docking Analysis of Some Active Compounds against Mycobacterium tuberculosis Receptor (Mtb CYP121). 针对结核分枝杆菌受体（Mtb CYP121）的一些活性化合物的 QSAR 建模和分子对接分析

IF 2.6

Journal of Pathogens Pub Date : 2018-05-10 eCollection Date: 2018-01-01 DOI: 10.1155/2018/1018694

Shola Elijah Adeniji, Sani Uba, Adamu Uzairu

{"title":"QSAR Modeling and Molecular Docking Analysis of Some Active Compounds against Mycobacterium tuberculosis Receptor (Mtb CYP121).","authors":"Shola Elijah Adeniji, Sani Uba, Adamu Uzairu","doi":"10.1155/2018/1018694","DOIUrl":"10.1155/2018/1018694","url":null,"abstract":"A quantitative structure-activity relationship (QSAR) study was performed to develop a model that relates the structures of 50 compounds to their activities against M. tuberculosis. The compounds were optimized by employing density functional theory (DFT) with B3LYP/6-31G⁎. The Genetic Function Algorithm (GFA) was used to select the descriptors and to generate the correlation model that relates the structural features of the compounds to their biological activities. The optimum model has squared correlation coefficient (R2) of 0.9202, adjusted squared correlation coefficient (Radj) of 0.91012, and leave-one-out (LOO) cross-validation coefficient (Qcv2) value of 0.8954. The external validation test used for confirming the predictive power of the built model has R2pred value of 0.8842. These parameters confirm the stability and robustness of the model. Docking analysis showed the best compound with high docking affinity of -14.6 kcal/mol which formed hydrophobic interaction and hydrogen bond with amino acid residues of M. tuberculosis cytochromes (Mtb CYP121). QSAR and molecular docking studies provide valuable approach for pharmaceutical and medicinal chemists to design and synthesize new anti-Mycobacterium tuberculosis compounds.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36188748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran. 伊朗马什哈德零售鸡肉中小肠结肠炎耶尔森菌的检测。

IF 2.6

Journal of Pathogens Pub Date : 2018-04-19 eCollection Date: 2018-01-01 DOI: 10.1155/2018/1286216

Khadigeh Sirghani, Tayebeh Zeinali, Abdollah Jamshidi

{"title":"Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran.","authors":"Khadigeh Sirghani, Tayebeh Zeinali, Abdollah Jamshidi","doi":"10.1155/2018/1286216","DOIUrl":"https://doi.org/10.1155/2018/1286216","url":null,"abstract":"Poultry meat is one of the most important sources of infection of Yersinia spp. for humans. The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay. Also, biochemical methods were used for biotyping. A total of 100 chicken thigh meat samples were collected randomly from retail outlets in Mashhad, Iran. Samples were enriched in Peptone-Sorbitol-Bile (PSB) broth and then cultured on Cefsulodin-Irgasan-Novobiocin (CIN) agar containing antibiotics supplement. The DNA was extracted from suspected colonies of Yersinia spp. and then PCR test using specific primers for 16S rRNA gene of Yersinia enterocolitica was performed. In this study, 30% of chicken meat was contaminated with Yersinia spp. by culture method and 25% of chicken meat was contaminated with Yersinia enterocolitica. Biotyping of isolated colonies showed that all of the isolates belonged to biotype 1A. Culture and detection of Yersinia spp. from food samples traditionally take 4 days. Due to high accuracy and speed of PCR assay, it is a good alternative method for microbiological techniques. In conclusion, poultry meat can act as a source of Y. enterocolitica and could be considered as a public health hazard.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/1286216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36178367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Prevalence of Multidrug-Resistant Tuberculosis and Associated Factors in Ethiopia: A Systematic Review. 埃塞俄比亚耐多药结核病患病率及相关因素:一项系统综述。

IF 2.6

Journal of Pathogens Pub Date : 2018-04-03 eCollection Date: 2018-01-01 DOI: 10.1155/2018/7104921

Solomon Weldegebreal Asgedom, Mebrahtu Teweldemedhin, Hailay Gebreyesus

{"title":"Prevalence of Multidrug-Resistant Tuberculosis and Associated Factors in Ethiopia: A Systematic Review.","authors":"Solomon Weldegebreal Asgedom, Mebrahtu Teweldemedhin, Hailay Gebreyesus","doi":"10.1155/2018/7104921","DOIUrl":"https://doi.org/10.1155/2018/7104921","url":null,"abstract":"Background: Multidrug-resistant tuberculosis (MDR-TB) has continued to be a challenge for tuberculosis (TB) control globally. Ethiopia is one of the countries with high MDR-TB burden.Objective: The main purpose of this study was to determine the prevalence of MDR-TB and associated factors in Ethiopia.Methods: A systematic review of the literatures on prevalence of MDR-TB and associated factors was conducted in the country.Results: In our electronic search, 546 citations were depicted. Among the total 546 citations described, a total of 22 articles met eligibility criteria and were included in the review article. According to our review, the prevalence of MDR-TB ranged from 0 to 46.3%. The average mean rate of MDR-TB in Ethiopia was found to be 12.6 ± 15.9%. The overall prevalence of MDR-TB in all TB cases was estimated to be 1.4%. From a total of 3849 patients studied, 527 had MDR-TB. Previous exposure to antituberculosis treatment was the most commonly identified risk factor of MDR-TB in Ethiopia.Conclusion: Despite relative decline in incidence of MDR-TB, the distribution and prevalence of MDR-TB continued to be a serious challenge for TB control in Ethiopia. Previous exposure to antituberculosis treatment was also the most common risk factor for MDR-TB. Therefore, strong TB and MDR-TB treatment along with tight introduction of follow-up strategies should be applied for better TB control.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/7104921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36178368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Stress-Adaptive Responses Associated with High-Level Carbapenem Resistance in KPC-Producing Klebsiella pneumoniae. 产kpc肺炎克雷伯菌与高水平碳青霉烯耐药相关的应激适应反应

IF 2.6

Journal of Pathogens Pub Date : 2018-03-19 eCollection Date: 2018-01-01 DOI: 10.1155/2018/3028290

Sheila Adams-Sapper, Adam Gayoso, Lee W Riley

{"title":"Stress-Adaptive Responses Associated with High-Level Carbapenem Resistance in KPC-Producing Klebsiella pneumoniae.","authors":"Sheila Adams-Sapper, Adam Gayoso, Lee W Riley","doi":"10.1155/2018/3028290","DOIUrl":"https://doi.org/10.1155/2018/3028290","url":null,"abstract":"Carbapenem-resistant Enterobacteriaceae (CRE) organisms have emerged to become a major global public health threat among antimicrobial resistant bacterial human pathogens. Little is known about how CREs emerge. One characteristic phenotype of CREs is heteroresistance, which is clinically associated with treatment failure in patients given a carbapenem. Through in vitro whole-transcriptome analysis we tracked gene expression over time in two different strains (BR7, BR21) of heteroresistant KPC-producing Klebsiella pneumoniae, first exposed to a bactericidal concentration of imipenem followed by growth in drug-free medium. In both strains, the immediate response was dominated by a shift in expression of genes involved in glycolysis toward those involved in catabolic pathways. This response was followed by global dampening of transcriptional changes involving protein translation, folding and transport, and decreased expression of genes encoding critical junctures of lipopolysaccharide biosynthesis. The emerged high-level carbapenem-resistant BR21 subpopulation had a prophage (IS1) disrupting ompK36 associated with irreversible OmpK36 porin loss. On the other hand, OmpK36 loss in BR7 was reversible. The acquisition of high-level carbapenem resistance by the two heteroresistant strains was associated with distinct and shared stepwise transcriptional programs. Carbapenem heteroresistance may emerge from the most adaptive subpopulation among a population of cells undergoing a complex set of stress-adaptive responses.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3028290","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36012619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Nonphotodynamic Roles of Methylene Blue: Display of Distinct Antimycobacterial and Anticandidal Mode of Actions. 亚甲基蓝的非光动力作用:显示出不同的抗细菌和抗细菌的作用模式。

IF 2.6

Journal of Pathogens Pub Date : 2018-01-31 eCollection Date: 2018-01-01 DOI: 10.1155/2018/3759704

Rahul Pal, Moiz A Ansari, Venkata Saibabu, Shrayanee Das, Zeeshan Fatima, Saif Hameed

{"title":"Nonphotodynamic Roles of Methylene Blue: Display of Distinct Antimycobacterial and Anticandidal Mode of Actions.","authors":"Rahul Pal, Moiz A Ansari, Venkata Saibabu, Shrayanee Das, Zeeshan Fatima, Saif Hameed","doi":"10.1155/2018/3759704","DOIUrl":"https://doi.org/10.1155/2018/3759704","url":null,"abstract":"Significance of methylene blue (MB) in photodynamic therapy against microbes is well established. Previously, we have reported the antifungal potential of MB against Candida albicans. The present study attempts to identify additional antimicrobial effect of MB against another prevalent human pathogen, Mycobacterium tuberculosis (MTB). We explored that MB is efficiently inhibiting the growth of Mycobacterium at 15.62 μg/ml albeit in bacteriostatic manner similar to its fungistatic nature. We uncovered additional cell surface phenotypes (colony morphology and cell sedimentation rate) which were impaired only in Mycobacterium. Mechanistic insights revealed that MB causes energy dependent membrane perturbation in both C. albicans and Mycobacterium. We also confirmed that MB leads to enhanced reactive oxygen species generation in both organisms that could be reversed upon antioxidant supplementation; however, DNA damage could only be observed in Mycobacterium. We provided evidence that although biofilm formation was disrupted in both organisms, cell adherence to human epithelial cells was inhibited only in Mycobacterium. Lastly, RT-PCR results showed good correlation with the biochemical assay. Together, apart from the well-established role of MB in photodynamic therapy, this study provides insights into the distinct antimicrobial mode of actions in two significant human pathogens, Candida and Mycobacterium, which can be extrapolated to improve our understanding of finding novel therapeutic options.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3759704","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36019970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

First Report of bla_CTX-M-28 in Enterobacteriaceae Isolates in the United Arab Emirates. 阿拉伯联合酋长国肠杆菌科分离物中blaCTX-M-28首次报道。

IF 2.6

Journal of Pathogens Pub Date : 2018-01-18 eCollection Date: 2018-01-01 DOI: 10.1155/2018/1304793

Mubarak Alfaresi, Garwin Kim Sing, Abiola Senok

{"title":"First Report of blaCTX-M-28 in Enterobacteriaceae Isolates in the United Arab Emirates.","authors":"Mubarak Alfaresi, Garwin Kim Sing, Abiola Senok","doi":"10.1155/2018/1304793","DOIUrl":"https://doi.org/10.1155/2018/1304793","url":null,"abstract":"Background: The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 blaCTX-M gene variants with the predominance of blaCTX-M-15 in many regions. In this report, we present the first description of blaCTX-M-28 in the United Arab Emirates.Methods: Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studied. Gene sequencing was performed and DNA sequences were annotated using the BLAST program to identify the gene subtypes.Results: The majority of the ESBL positive isolates were E. coli (n/N = 39/45; 86.6%) followed by K. pneumoniae (n = 5) and K. oxytoca (n = 1). All isolates harboured blaCTX-M and blaTEM genes, 18 had blaSHV, and 2 were blaVIM positive. Thirty-seven isolates (82.2%) were positive for blaCTX-M-28. Other blaCTX-M genes identified include blaCTX-M-167 (n = 2; isolates #1 and 26) and one each for blaCTX-M-38, blaCTX-M-163, and blaCTX-M-198. No blaCTX-M-15 was identified. The predominant blaTEM subtype was blaTEM-171 (n = 8) followed by one of each of blaTEM-120, blaTEM-163, and blaTEM-206. The blaSHV subtypes were blaSHV-148 and blaSHV-187.Conclusion: The findings indicate the first description of blaCTX-M-28 in a setting where blaCTX-M-15 was previously predominant.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/1304793","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35957160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Antimicrobial Susceptibility among Colistin, Sulbactam, and Fosfomycin and a Synergism Study of Colistin in Combination with Sulbactam or Fosfomycin against Clinical Isolates of Carbapenem-Resistant Acinetobacter baumannii. 粘菌素、舒巴坦和磷霉素的药敏及粘菌素联合舒巴坦或磷霉素对耐碳青霉烯鲍曼不动杆菌临床分离株的增效研究。

IF 2.6

Journal of Pathogens Pub Date : 2018-01-18 eCollection Date: 2018-01-01 DOI: 10.1155/2018/3893492

Sombat Leelasupasri, Wichai Santimaleeworagun, Tossawan Jitwasinkul

{"title":"Antimicrobial Susceptibility among Colistin, Sulbactam, and Fosfomycin and a Synergism Study of Colistin in Combination with Sulbactam or Fosfomycin against Clinical Isolates of Carbapenem-Resistant Acinetobacter baumannii.","authors":"Sombat Leelasupasri, Wichai Santimaleeworagun, Tossawan Jitwasinkul","doi":"10.1155/2018/3893492","DOIUrl":"https://doi.org/10.1155/2018/3893492","url":null,"abstract":"This in vitro study aimed to determine the activity of colistin plus sulbactam and colistin plus fosfomycin against carbapenem-resistant A. baumannii (CRAB). Fifteen clinical isolates were obtained from patients admitted to Phyathai II International Hospital, Bangkok, Thailand, from August 2014 to April 2015. The antimicrobial susceptibilities of colistin, sulbactam, and fosfomycin were evaluated using the E-test or broth microdilution and the synergistic activity of the antibacterial combinations (colistin plus sulbactam or fosfomycin) was determined using the chequerboard method. Clonal relationships were explored using repetitive element palindromic- (REP-) PCR. The CRAB isolates were categorized by REP-PCR in 8 groups [A-H]. All CRAB isolates were universally susceptible to colistin but only 20.0% were susceptible to sulbactam. The MIC ranges for colistin, sulbactam, and fosfomycin were 0.75–2 mg/L, 2–96 mg/L, and 64–256 mg/L, respectively. A chequerboard assay revealed that the rates of synergistic and additive effect rates of colistin plus sulbactam and colistin plus fosfomycin were 53.3% and 73.3% of isolates, respectively. No antagonistic effect in any colistin-based combination was observed. However, almost CRAB strains in clone A showed the synergy or additive effects of colistin-sulbactam combination, whereas the other clone (B-H) mostly showed indifferent effects. In conclusion, colistin plus sulbactam and colistin plus fosfomycin against CRAB seem to be interesting option but the efficacy in clinical use has to be evaluated.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3893492","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35957161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Molecular Detection of Brucella spp. from Milk of Seronegative Cows from Some Selected Area in Bangladesh. 孟加拉部分地区血清阴性奶牛乳中布鲁氏菌的分子检测。

IF 2.6

Journal of Pathogens Pub Date : 2018-01-14 eCollection Date: 2018-01-01 DOI: 10.1155/2018/9378976

Md Sadequl Islam, Md Ariful Islam, Mst Minara Khatun, Sukumar Saha, Md Samiul Basir, Md-Mahmodul Hasan

{"title":"Molecular Detection of Brucella spp. from Milk of Seronegative Cows from Some Selected Area in Bangladesh.","authors":"Md Sadequl Islam, Md Ariful Islam, Mst Minara Khatun, Sukumar Saha, Md Samiul Basir, Md-Mahmodul Hasan","doi":"10.1155/2018/9378976","DOIUrl":"10.1155/2018/9378976","url":null,"abstract":"Brucellosis is endemic in Bangladesh both in humans and in animals. A number of reasons complicate the diagnosis, as bovine brucellosis can be diagnosed by various serological tests. But the tests have a limitation; when the organism remains intracellular, the disease goes into chronic stage and the antibody titres may decline. The present study was conducted for isolation and detection of Brucella spp. by polymerase chain reaction (PCR) from seronegative cows. A total of 360 dairy cows from three geographical regions were screened serologically by Rose Bengal Plate Test (RBPT) where 24 samples were serologically positive and the rest of the samples were serologically negative. Among the 24 seropositive individuals, 11 were culture positive and 6 were culture positive from serologically negative dairy cows. The overall seroprevalence of brucellosis in cattle was 6.6% and in disease condition a higher prevalence was recorded in abortion (28.07%) followed by infertility (13.33%). To confirm the Brucella spp. in seronegative dairy cattle, the isolates were extracted and PCR was conducted, which produced 905 bp amplicon size of 6 Brucella spp. from milk sample. So, for the detection or eradication of brucellosis, a bacteriological test and a PCR technique should be performed with the serological test of milk.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/9378976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35940666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis 急性弛缓性麻痹患儿粪便中肠道病毒检测算法的比较

IF 2.6

Journal of Pathogens Pub Date : 2017-08-23 DOI: 10.1101/179721

J. Adeniji, Funmilola A. Ayeni, Abdulfatah Ibrahim, Kazeem A. Tijani, Kazeem A. Tijani, T. Faleye, T. Faleye, M. Adewumi

{"title":"Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis","authors":"J. Adeniji, Funmilola A. Ayeni, Abdulfatah Ibrahim, Kazeem A. Tijani, Kazeem A. Tijani, T. Faleye, T. Faleye, M. Adewumi","doi":"10.1101/179721","DOIUrl":"https://doi.org/10.1101/179721","url":null,"abstract":"With poliovirus eradication within reach, the WHO has included in its recommendations a cell-culture independent algorithm for enterovirus surveillance. This study was designed to compare both the cell culture dependent and independent algorithms and assess how either might impact our perception of the diversity of enterovirus types present in a sample. Sixteen paired samples (16 isolates from RD cell culture and their corresponding stool suspension. i.e. 32 samples) from AFP cases in Nigeria were analyzed in this study. One of these 16 sample pairs (the control) was previously identified and confirmed as poliovirus 2 (PV-2). All the samples were subjected to RNA extraction, cDNA synthesis, RT-snPCR (the WHO recommended cell-culture independent algorithm) and its modifications for co-infection detection and resolution. Amplicons were sequenced and strains identified using the enterovirus genotyping tool and phylogenetic analysis. The enterovirus diversity was shown to be the same between RD cell culture isolates and fecal suspension for the control and five (7, 10, 11, 12 & 14) of the samples analyzed. It was however, different for the remaining 10 (62.5%) samples analyzed. Fourteen different enterovirus types were identified in this study. To be precise, 9 (CV-B4, E6, E7, E13, E14, E19, E29, EV-B75 and EV-B77) and 5 (CV-A1, CV-A11, CV-A13, EV-C99 and PV2) EV-B and EV-C types, respectively where detected in this study. It is crucial to mention that E19 and EV-B75were only recovered from RD cell culture isolates while E14, EV-B77, CV-A11 and CV-A13 were only recovered from fecal suspension. The results of this study show that both the cell culture dependent and independent protocols recommended by the WHO for enterovirus detection unavoidably bias our perception of the diversity of enterovirus types present in a sample. Hence, rather than jettison one for the other, effort should be directed at harmonizing both for increased sensitivity.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45884340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4