阿拉伯联合酋长国肠杆菌科分离物中blaCTX-M-28首次报道。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2018-01-18 eCollection Date: 2018-01-01 DOI:10.1155/2018/1304793

Mubarak Alfaresi, Garwin Kim Sing, Abiola Senok

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引用次数: 9

摘要

背景:扩展谱β -内酰胺酶(ESBL) CTX-M家族由60多个blaCTX-M基因变体组成，在许多地区以blaCTX-M-15基因为主。在本报告中，我们首次介绍了阿拉伯联合酋长国的blaCTX-M-28。方法:对2016年6月至7月在阿拉伯联合酋长国一家二级医疗机构发现的45株非重复ESBL产生分离株进行研究。进行基因测序，并使用BLAST程序对DNA序列进行注释，以确定基因亚型。结果:ESBL阳性分离株以大肠杆菌为主(n/ n = 39/45;86.6%)，其次是肺炎克雷伯菌(n = 5)和oxytoca克雷伯菌(n = 1)。所有分离株均携带blaCTX-M和blaTEM基因，其中blaSHV 18株，blaVIM 2株阳性。blaCTX-M-28阳性37株(82.2%);其他已鉴定的blaCTX-M基因包括blaCTX-M-167 (n = 2;分离株#1和26)，blaCTX-M-38、blaCTX-M-163和blaCTX-M-198各1株。未发现blaCTX-M-15。主要亚型为blatem1 1 1 (n = 8)，其次为blatem1 2 2、blatem1 2 3和blatem1 2 6。blaSHV亚型分别为blaSHV-148和blaSHV-187。结论:研究结果表明，在blaCTX-M-15之前占优势的情况下，blaCTX-M-28首次被描述。

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First Report of bla_CTX-M-28 in Enterobacteriaceae Isolates in the United Arab Emirates.

Background: The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 bla_CTX-M gene variants with the predominance of bla_CTX-M-15 in many regions. In this report, we present the first description of bla_CTX-M-28 in the United Arab Emirates.

Methods: Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studied. Gene sequencing was performed and DNA sequences were annotated using the BLAST program to identify the gene subtypes.

Results: The majority of the ESBL positive isolates were E. coli (n/N = 39/45; 86.6%) followed by K. pneumoniae (n = 5) and K. oxytoca (n = 1). All isolates harboured bla_CTX-M and bla_TEM genes, 18 had bla_SHV, and 2 were bla_VIM positive. Thirty-seven isolates (82.2%) were positive for bla_CTX-M-28. Other bla_CTX-M genes identified include bla_CTX-M-167 (n = 2; isolates #1 and 26) and one each for bla_CTX-M-38, bla_CTX-M-163, and bla_CTX-M-198. No bla_CTX-M-15 was identified. The predominant bla_TEM subtype was bla_TEM-171 (n = 8) followed by one of each of bla_TEM-120, bla_TEM-163, and bla_TEM-206. The bla_SHV subtypes were bla_SHV-148 and bla_SHV-187.

Conclusion: The findings indicate the first description of bla_CTX-M-28 in a setting where bla_CTX-M-15 was previously predominant.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.