{"title":"阿拉伯联合酋长国肠杆菌科分离物中blaCTX-M-28首次报道。","authors":"Mubarak Alfaresi, Garwin Kim Sing, Abiola Senok","doi":"10.1155/2018/1304793","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 <i>bla</i><sub>CTX-M</sub> gene variants with the predominance of <i>bla</i><sub>CTX-M-15</sub> in many regions. In this report, we present the first description of <i>bla</i><sub>CTX-M-28</sub> in the United Arab Emirates.</p><p><strong>Methods: </strong>Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studied. Gene sequencing was performed and DNA sequences were annotated using the BLAST program to identify the gene subtypes.</p><p><strong>Results: </strong>The majority of the ESBL positive isolates were <i>E. coli</i> (<i>n</i>/<i>N</i> = 39/45; 86.6%) followed by <i>K. pneumoniae</i> (<i>n</i> = 5) and <i>K. oxytoca</i> (<i>n</i> = 1). All isolates harboured <i>bla</i><sub>CTX-M</sub> and <i>bla</i><sub>TEM</sub> genes, 18 had <i>bla</i><sub>SHV</sub>, and 2 were <i>bla</i><sub>VIM</sub> positive. Thirty-seven isolates (82.2%) were positive for <i>bla</i><sub>CTX-M-28</sub>. Other <i>bla</i><sub>CTX-M</sub> genes identified include <i>bla</i><sub>CTX-M-167</sub> (<i>n</i> = 2; isolates #1 and 26) and one each for <i>bla</i><sub>CTX-M-38</sub>, <i>bla</i><sub>CTX-M-163</sub>, and <i>bla</i><sub>CTX-M-198</sub>. No <i>bla</i><sub>CTX-M-15</sub> was identified. The predominant <i>bla</i><sub>TEM</sub> subtype was <i>bla</i><sub>TEM-171</sub> (<i>n</i> = 8) followed by one of each of <i>bla</i><sub>TEM-120</sub>, <i>bla</i><sub>TEM-163</sub>, and <i>bla</i><sub>TEM-206</sub>. The <i>bla</i><sub>SHV</sub> subtypes were <i>bla</i><sub>SHV-148</sub> and <i>bla</i><sub>SHV-187</sub>.</p><p><strong>Conclusion: </strong>The findings indicate the first description of <i>bla</i><sub>CTX-M-28</sub> in a setting where <i>bla</i><sub>CTX-M-15</sub> was previously predominant.</p>","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2018 ","pages":"1304793"},"PeriodicalIF":1.1000,"publicationDate":"2018-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/1304793","citationCount":"9","resultStr":"{\"title\":\"First Report of <i>bla</i><sub>CTX-M-28</sub> in Enterobacteriaceae Isolates in the United Arab Emirates.\",\"authors\":\"Mubarak Alfaresi, Garwin Kim Sing, Abiola Senok\",\"doi\":\"10.1155/2018/1304793\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 <i>bla</i><sub>CTX-M</sub> gene variants with the predominance of <i>bla</i><sub>CTX-M-15</sub> in many regions. In this report, we present the first description of <i>bla</i><sub>CTX-M-28</sub> in the United Arab Emirates.</p><p><strong>Methods: </strong>Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studied. Gene sequencing was performed and DNA sequences were annotated using the BLAST program to identify the gene subtypes.</p><p><strong>Results: </strong>The majority of the ESBL positive isolates were <i>E. coli</i> (<i>n</i>/<i>N</i> = 39/45; 86.6%) followed by <i>K. pneumoniae</i> (<i>n</i> = 5) and <i>K. oxytoca</i> (<i>n</i> = 1). All isolates harboured <i>bla</i><sub>CTX-M</sub> and <i>bla</i><sub>TEM</sub> genes, 18 had <i>bla</i><sub>SHV</sub>, and 2 were <i>bla</i><sub>VIM</sub> positive. Thirty-seven isolates (82.2%) were positive for <i>bla</i><sub>CTX-M-28</sub>. Other <i>bla</i><sub>CTX-M</sub> genes identified include <i>bla</i><sub>CTX-M-167</sub> (<i>n</i> = 2; isolates #1 and 26) and one each for <i>bla</i><sub>CTX-M-38</sub>, <i>bla</i><sub>CTX-M-163</sub>, and <i>bla</i><sub>CTX-M-198</sub>. No <i>bla</i><sub>CTX-M-15</sub> was identified. The predominant <i>bla</i><sub>TEM</sub> subtype was <i>bla</i><sub>TEM-171</sub> (<i>n</i> = 8) followed by one of each of <i>bla</i><sub>TEM-120</sub>, <i>bla</i><sub>TEM-163</sub>, and <i>bla</i><sub>TEM-206</sub>. The <i>bla</i><sub>SHV</sub> subtypes were <i>bla</i><sub>SHV-148</sub> and <i>bla</i><sub>SHV-187</sub>.</p><p><strong>Conclusion: </strong>The findings indicate the first description of <i>bla</i><sub>CTX-M-28</sub> in a setting where <i>bla</i><sub>CTX-M-15</sub> was previously predominant.</p>\",\"PeriodicalId\":16788,\"journal\":{\"name\":\"Journal of Pathogens\",\"volume\":\"2018 \",\"pages\":\"1304793\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2018-01-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2018/1304793\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pathogens\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2018/1304793\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2018/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pathogens","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2018/1304793","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
First Report of blaCTX-M-28 in Enterobacteriaceae Isolates in the United Arab Emirates.
Background: The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 blaCTX-M gene variants with the predominance of blaCTX-M-15 in many regions. In this report, we present the first description of blaCTX-M-28 in the United Arab Emirates.
Methods: Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studied. Gene sequencing was performed and DNA sequences were annotated using the BLAST program to identify the gene subtypes.
Results: The majority of the ESBL positive isolates were E. coli (n/N = 39/45; 86.6%) followed by K. pneumoniae (n = 5) and K. oxytoca (n = 1). All isolates harboured blaCTX-M and blaTEM genes, 18 had blaSHV, and 2 were blaVIM positive. Thirty-seven isolates (82.2%) were positive for blaCTX-M-28. Other blaCTX-M genes identified include blaCTX-M-167 (n = 2; isolates #1 and 26) and one each for blaCTX-M-38, blaCTX-M-163, and blaCTX-M-198. No blaCTX-M-15 was identified. The predominant blaTEM subtype was blaTEM-171 (n = 8) followed by one of each of blaTEM-120, blaTEM-163, and blaTEM-206. The blaSHV subtypes were blaSHV-148 and blaSHV-187.
Conclusion: The findings indicate the first description of blaCTX-M-28 in a setting where blaCTX-M-15 was previously predominant.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
