Journal of Pathogens最新文献_第7页

Temporal Regulation of fim Genes in Uropathogenic Escherichia coli during Infection of the Murine Urinary Tract. 尿路致病性大肠杆菌感染过程中膜基因的时间调控。

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-12-27 DOI: 10.1155/2017/8694356

William R Schwan, Hua Ding

{"title":"Temporal Regulation of fim Genes in Uropathogenic Escherichia coli during Infection of the Murine Urinary Tract.","authors":"William R Schwan, Hua Ding","doi":"10.1155/2017/8694356","DOIUrl":"https://doi.org/10.1155/2017/8694356","url":null,"abstract":"Uropathogenic Escherichia coli (UPEC) adhere to cells in the human urinary tract via type 1 pili that undergo phase variation where a 314-bp fimS DNA element flips between Phase-ON and Phase-OFF orientations through two site-specific recombinases, FimB and FimE. Three fim-lux operon transcriptional fusions were created and moved into the clinical UPEC isolate NU149 to determine their temporal regulation in UPEC growing in the urinary tract. Within murine urinary tracts, the UPEC strains demonstrated elevated transcription of fimA and fimB early in the infection, but lower transcription by the fifth day in murine kidneys. In contrast, fimE transcription was much lower than either fimA or fimB early, increased markedly at 24 h after inoculation, and then dropped five days after inoculation. Positioning of fimS was primarily in the Phase-ON position over the time span in UPEC infected bladders, whereas in UPEC infected murine kidneys the Phase-OFF orientation was favored by the fifth day after inoculation. Hemagglutination titers with guinea pig erythrocytes remained constant in UPEC growing in infected murine bladders but fell substantially in UPEC infected kidneys over time. Our results show temporal in vivo regulation of fim gene expression in different environmental niches when UPEC infects the murine urinary tract.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/8694356","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35831979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

High Burden of Antimicrobial Resistance among Bacteria Causing Pyogenic Wound Infections at a Tertiary Care Hospital in Kathmandu, Nepal. 在尼泊尔加德满都的一家三级护理医院，引起化脓性伤口感染的细菌的高抗菌素耐药性负担。

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-08-28 DOI: 10.1155/2017/9458218

Basista Prasad Rijal, Deepa Satyal, Narayan Prasad Parajuli

{"title":"High Burden of Antimicrobial Resistance among Bacteria Causing Pyogenic Wound Infections at a Tertiary Care Hospital in Kathmandu, Nepal.","authors":"Basista Prasad Rijal, Deepa Satyal, Narayan Prasad Parajuli","doi":"10.1155/2017/9458218","DOIUrl":"https://doi.org/10.1155/2017/9458218","url":null,"abstract":"Pyogenic wound infections are one of the most common clinical entities caused and aggravated by the invasion of pathogenic organisms. Prompt and aggressive antimicrobial therapy is needed to reduce the burden and complications associated with these infections. In this study, we intended to investigate the common pathogens and their antimicrobial susceptibility patterns from the pyogenic wound infections at a tertiary care hospital in Kathmandu, Nepal. A laboratory based cross-sectional study was carried out among the pyogenic clinical specimens of the patients visiting Manmohan Memorial Teaching Hospital, Kathmandu, Nepal. Processing of clinical specimens and isolation and identification of bacterial pathogens were carried out using standard microbiological methods. Antimicrobial susceptibilities and resistant profiles were determined by following the standard guidelines of Clinical and Laboratory Standards Institute (CLSI). About 65% of the clinical specimens were positive for the bacterial growth and Gram positive bacteria (57.4%) were the leading pathogens among pyogenic wound infections. Staphylococcus aureus (412, 49.28%), Escherichia coli (136, 16.27%), Klebsiella spp. (88, 10.53%), and Pseudomonas spp. (44, 5.26%) were the common pathogens isolated. High level of drug resistance was observed among both Gram positive bacteria (51.9%) and Gram negative bacteria (48.7%). Gram positive isolates were resistant to ampicillin, ciprofloxacin, cotrimoxazole, erythromycin, and cloxacillin. Gram negative isolates were resistant to cephalosporins but were well susceptible to amikacin and imipenem. Pyogenic wound infections are common in our hospital and majority of them were associated with multidrug resistant bacteria. The detailed workup of the prevalent pathogens present in infected wounds and their resistance pattern is clearly pertinent to choosing the adequate treatment.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/9458218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35531988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Histoplasmosis by Histoplasma capsulatum var. duboisii Observed at the Laboratory of Pathological Anatomy of Lomé in Togo. 多哥lomoise病理解剖实验室观察到的杜布依氏荚膜组织浆体病。

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-07-18 DOI: 10.1155/2017/2323412

Tchin Darré, Bayaki Saka, Abas Mouhari-Touré, Améyo Monique Dorkenoo, Koffi Amégbor, Vincent Palokinam Pitche, Gado Napo-Koura

{"title":"Histoplasmosis by Histoplasma capsulatum var. duboisii Observed at the Laboratory of Pathological Anatomy of Lomé in Togo.","authors":"Tchin Darré, Bayaki Saka, Abas Mouhari-Touré, Améyo Monique Dorkenoo, Koffi Amégbor, Vincent Palokinam Pitche, Gado Napo-Koura","doi":"10.1155/2017/2323412","DOIUrl":"https://doi.org/10.1155/2017/2323412","url":null,"abstract":"Our study aimed to describe the epidemiological, clinical, and diagnostic aspects of African histoplasmosis in Togo through a descriptive and cross-sectional study on histological diagnosed African histoplasmosis in Pathology Department of Lomé from 2002 to 2016 (15 years). A total of 17 cases of African histoplasmosis were diagnosed. The sex ratio (M/F) was 1.8. The annual incidence was 1.1 cases. The mean age of the patients was 27.2 ± 0.4 years. All our patients were of social categories with a low socioeconomic level. HIV infection was known in 3 patients and one patient contracted tuberculosis. The clinical manifestations were cutaneous in 7 cases, cutaneous and mucous in 3 cases, cutaneous and lymph node in 3 cases, cutaneous and bone in 2 cases, and disseminated in 2 cases. The samples examined consisted of 14 cutaneous biopsies measuring 2-3 cm and 3 ganglionic biopsies each measuring 4 cm of major axis. Histologically, all cases were of chronic form made of granulomatous reaction with ovoid yeasts measuring between 1 and 2 microns. Despite the low frequency of this disease in our country, it should be kept constantly in mind before any granulomatous lesions, especially in the context of the HIV pandemic.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/2323412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35265185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Evaluation of CAMP-Like Effect, Biofilm Formation, and Discrimination of Candida africana from Vaginal Candida albicans Species. camp样效应、生物膜形成及非洲念珠菌与阴道白色念珠菌鉴别的评价。

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-11-26 DOI: 10.1155/2017/7126258

Keyvan Pakshir, Mahboubeh Bordbar, Kamiar Zomorodian, Hasti Nouraei, Hossein Khodadadi

{"title":"Evaluation of CAMP-Like Effect, Biofilm Formation, and Discrimination of Candida africana from Vaginal Candida albicans Species.","authors":"Keyvan Pakshir, Mahboubeh Bordbar, Kamiar Zomorodian, Hasti Nouraei, Hossein Khodadadi","doi":"10.1155/2017/7126258","DOIUrl":"https://doi.org/10.1155/2017/7126258","url":null,"abstract":"Candida africana as a species recovered from female genital specimens is highly close to C. albicans. The present study was conducted to discriminate C. africana from presumptive vaginal C. albicans strains by molecular assay and evaluate their hemolysin activity, biofilm formation, and cohemolytic effect (CAMP) with vaginal bacterial flora. A total of 110 stock vaginal C. albicans isolates were examined by HWP1 gene amplification. Hemolysin activity and the ability of biofilm formation were evaluated by blood plate assay and visual detection methods, respectively. Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus agalactiae were used to evaluate the CAMP-like effects in Sabouraud blood agar media. Based on the size of the amplicons (941 bp), all isolates were identified as C. albicans. All samples were able to produce beta-hemolysin. Moreover, 69 out of 110 of the isolates (62.7%) were biofilm-positive, 54 out of 110 Candida isolates (49%) demonstrated cohemolytic effects with S. agalactiae, and 48 out of 110 showed this effect with S. aureus (43.6%). All isolates were CAMP-negative with S. epidermidis. We detected all isolates as Candida albicans and almost half of the isolates were CAMP-positive with S. aureus and S. agalactiae, suggesting that these bacteria increase the pathogenicity of Candida in vaginal candidiasis.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/7126258","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35723027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Epstein-Barr Virus as a Promising Immunotherapeutic Target for Nasopharyngeal Carcinoma Treatment. 将 Epstein-Barr 病毒作为治疗鼻咽癌的有望免疫治疗靶点

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-12-31 DOI: 10.1155/2017/7349268

Sin-Yeang Teow, Hooi-Yeen Yap, Suat-Cheng Peh

引用次数: 0

Acute Hepatitis E Virus Infection in Two Geographical Regions of Nigeria. 尼日利亚两个地理区域的急性戊型肝炎病毒感染。

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-12-13 DOI: 10.1155/2017/4067108

I M Ifeorah, T O C Faleye, A S Bakarey, M O Adewumi, A Akere, E C Omoruyi, A O Ogunwale, J A Adeniji

{"title":"Acute Hepatitis E Virus Infection in Two Geographical Regions of Nigeria.","authors":"I M Ifeorah, T O C Faleye, A S Bakarey, M O Adewumi, A Akere, E C Omoruyi, A O Ogunwale, J A Adeniji","doi":"10.1155/2017/4067108","DOIUrl":"10.1155/2017/4067108","url":null,"abstract":"Hepatitis E virus (HEV) remains a major public health concern in resource limited regions of the world. Yet data reporting is suboptimal and surveillance system is inadequate. In Nigeria, there is dearth of information on prevalence of acute HEV infection. This study was therefore designed to describe acute HEV infection among antenatal clinic attendees and community dwellers from two geographical regions in Nigeria. Seven hundred and fifty plasma samples were tested for HEV IgM by Enzyme Linked Immunosorbent Assay (ELISA) technique. The tested samples were randomly selected from a pool of 1,115 blood specimens previously collected for viral hepatitis studies among selected populations (pregnant women, 272; Oyo community dwellers, 438; Anambra community dwellers, 405) between September 2012 and August 2013. One (0.4%) pregnant woman in her 3rd trimester had detectable HEV IgM, while community dwellers from the two study locations had zero prevalence rates of HEV IgM. Detection of HEV IgM in a pregnant woman, especially in her 3rd trimester, is of clinical and epidemiological significance. The need therefore exists for establishment of a robust HEV surveillance system in Nigeria and especially amidst the pregnant population in a bid to improve maternal and child health.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/4067108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35781650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Molecular Epidemiology of Rifampicin Resistance in Mycobacterium tuberculosis Using the GeneXpert MTB/RIF Assay from a Rural Setting in India. 利用来自印度农村地区的GeneXpert MTB/RIF测定方法研究结核分枝杆菌对利福平耐药性的分子流行病学

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-10-26 DOI: 10.1155/2017/6738095

Raghuprakash Reddy, Gerardo Alvarez-Uria

{"title":"Molecular Epidemiology of Rifampicin Resistance in Mycobacterium tuberculosis Using the GeneXpert MTB/RIF Assay from a Rural Setting in India.","authors":"Raghuprakash Reddy, Gerardo Alvarez-Uria","doi":"10.1155/2017/6738095","DOIUrl":"https://doi.org/10.1155/2017/6738095","url":null,"abstract":"The Xpert MTB/RIF assay can detect mutations in rpoB gene that confer rifampicin resistance (RR) using five overlapping probes (A, B, C, D, and E). In this study, we described our experience with the Xpert assay in a rural setting in India. During the study period, 3250 samples were processed. The result was unsuccessful in 5.7% of cases. For extrapulmonary specimens, the risk of unsuccessful result was higher in tissue biopsy and stool samples. Among samples positive for Mycobacterium tuberculosis, rifampicin resistance was indeterminate in 1.2% of them. Our results and a review of the literature showed that the most frequent mutations conferring RR were located in the region of Probe E (63.6%; 95% confidence interval [CI] 56.26-70.94), followed by Probe B (15.02%; 95% CI 11.94-18.10), Probe D (13.35%; 95% CI 10.01-16.69), Probe A (4.73%; 95% CI 1.92-7.54), and Probe C (1.61%; 95% CI 0.67-2.54). Although the high cost of the cartridges precluded using the Xpert assay for routine diagnosis of tuberculosis, our results demonstrate that the assay can be used to diagnose RR-tuberculosis in rural areas with limited laboratory infrastructure and could be a convenient tool to investigate the molecular epidemiology of RR in resource-limited settings.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/6738095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35329269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Novel Genetic Variants of Hepatitis B Virus in Fulminant Hepatitis. 暴发性肝炎中乙型肝炎病毒的新基因变异。

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-12-19 DOI: 10.1155/2017/1231204

Jack Bee Chook, Yun Fong Ngeow, Kok Keng Tee, Suat Cheng Peh, Rosmawati Mohamed

{"title":"Novel Genetic Variants of Hepatitis B Virus in Fulminant Hepatitis.","authors":"Jack Bee Chook, Yun Fong Ngeow, Kok Keng Tee, Suat Cheng Peh, Rosmawati Mohamed","doi":"10.1155/2017/1231204","DOIUrl":"https://doi.org/10.1155/2017/1231204","url":null,"abstract":"Fulminant hepatitis (FH) is a life-threatening liver disease characterised by intense immune attack and massive liver cell death. The common precore stop codon mutation of hepatitis B virus (HBV), A1896, is frequently associated with FH, but lacks specificity. This study attempts to uncover all possible viral nucleotides that are specifically associated with FH through a compiled sequence analysis of FH and non-FH cases from acute infection. We retrieved 67 FH and 280 acute non-FH cases of hepatitis B from GenBank and applied support vector machine (SVM) model to seek candidate nucleotides highly predictive of FH. Six best candidates with top predictive accuracy, 92.5%, were used to build a SVM model; they are C2129 (85.3%), T720 (83.0%), Y2131 (82.4%), T2013 (82.1%), K2048 (82.1%), and A2512 (82.1%). This model gave a high specificity (99.3%), positive predictive value (95.6%), and negative predictive value (92.1%), but only moderate sensitivity (64.2%). We successfully built a SVM model comprising six variants that are highly predictive and specific for FH: four in the core region and one each in the polymerase and the surface regions. These variants indicate that intracellular virion/core retention could play an important role in the progression to FH.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/1231204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35802973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Comparative Response of the Nigerian Indigenous and Broiler Chickens to a Field Caecal Isolate of Eimeria Oocysts. 尼日利亚本土鸡和肉鸡对一株艾美耳球虫卵囊的反应比较。

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-04-24 DOI: 10.1155/2017/2674078

Lucas Atehmengo Ngongeh, Amaechi Onyeabor, Emeka Nzenwata, Gurama Kansalem Samson

{"title":"Comparative Response of the Nigerian Indigenous and Broiler Chickens to a Field Caecal Isolate of Eimeria Oocysts.","authors":"Lucas Atehmengo Ngongeh, Amaechi Onyeabor, Emeka Nzenwata, Gurama Kansalem Samson","doi":"10.1155/2017/2674078","DOIUrl":"https://doi.org/10.1155/2017/2674078","url":null,"abstract":"Response of Nigerian indigenous (local) and broiler chickens to experimental Eimeria infections was investigated by measures of clinical signs, packed cell volume (PCV), body weights (BW), feed consumption, faecal oocyst counts (oocyst per gram), and microscopic intestinal lesions. Three-week-old chickens of each breed received single pulse infections with 2500, 5000, and 100.000 sporulated Eimeria oocysts. Infected birds were dull and passed bloody diarrhoea. OPG showed a dose related response but no significant difference between groups (P > 0.05). OPG was significantly higher in local chickens (P < 0.05) and varied significantly with time (P < 0.05). PCV declined significantly in infected birds within breeds and groups (P < 0.05); however, the decline in PCV was significantly greater in broilers (P < 0.05). Both breeds had significant BW gains (P < 0.05). BW gain varied between groups being significantly higher in the uninfected control broilers than in the infected broilers (P < 0.05). Comparatively, broilers gained significantly more BW than their local counterparts (P < 0.05). Feed intake increased significantly with time (P < 0.05) in both breeds. The Eimeria isolate was pathogenic to both breeds of chicken although clinical signs and lesions were more severe in indigenous chickens suggesting the breed's more susceptibility.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/2674078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35009703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis. 急性弛缓性麻痹患儿粪便中肠道病毒检测算法的比较

IF 2.6

Journal of Pathogens Pub Date : 2017-01-01 Epub Date: 2017-12-28 DOI: 10.1155/2017/9256056

J A Adeniji, F A Ayeni, A Ibrahim, K A Tijani, T O C Faleye, M O Adewumi

{"title":"Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis.","authors":"J A Adeniji, F A Ayeni, A Ibrahim, K A Tijani, T O C Faleye, M O Adewumi","doi":"10.1155/2017/9256056","DOIUrl":"https://doi.org/10.1155/2017/9256056","url":null,"abstract":"This study was designed to compare both the cell culture dependent and independent enterovirus detection algorithms recommended by the WHO and assess how either might impact our perception of the diversity of enterovirus types present in a sample. Sixteen paired samples (16 isolates from RD cell culture and their corresponding stool suspension, i.e., 32 samples) from AFP cases in Nigeria were analyzed in this study. All the samples were subjected to RNA extraction, cDNA synthesis, the WHO recommended RT-snPCR, and its modification. Amplicons were sequenced and strains identified. Enterovirus diversity was the same between the isolates and fecal suspension for the control and five of the samples. It was, however, different for the remaining 10 (62.5%) samples. Nine (CV-B4, E6, E7, E13, E14, E19, E29, EV-B75, and EV-B77) and five (CV-A1, CV-A11, CV-A13, EV-C99, and PV2) EV-B and EV-C types, respectively, were detected. Particularly, E19 and EV-B75 were only recovered from the isolates while E14, EV-B77, CV-A11, and CV-A13 were only recovered from fecal suspension. Both the cell culture dependent and independent protocols bias our perception of the diversity of enterovirus types present in a sample. Hence, effort should be directed at harmonizing both for increased sensitivity.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/9256056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35831980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0