Journal of Pathogens最新文献_第10页

Comparative Response of the West African Dwarf Goats to Experimental Infections with Red Sokoto and West African Dwarf Goat Isolates of Haemonchus contortus 西非矮山羊对红索科托和西非矮山羊弯曲血蜱实验感染的反应比较

IF 2.6

Journal of Pathogens Pub Date : 2015-11-30 DOI: 10.1155/2015/728210

L. A. Ngongeh, A. Onyeabor

{"title":"Comparative Response of the West African Dwarf Goats to Experimental Infections with Red Sokoto and West African Dwarf Goat Isolates of Haemonchus contortus","authors":"L. A. Ngongeh, A. Onyeabor","doi":"10.1155/2015/728210","DOIUrl":"https://doi.org/10.1155/2015/728210","url":null,"abstract":"Response of the West African Dwarf (WAD) goats to two different isolates of Haemonchus contortus, the Red Sokoto (RS) goat isolate (RSHc) and the WAD goat isolate (WADHc) (isolated from WAD goats), was studied by experimental infections of 4–6-month-old male WAD goat kids. Group 1 and Group 2 goats were each infected with 4500 infective larvae (L3) of RSHc and WADHc, respectively. Group 3 animals served as uninfected control. Prepatent period (PPP), faecal egg counts (FEC), worm burden (WB), body weight (BW), packed cell volume (PCV), and body condition score (BCS) were determined. WAD goats infected with RSHc isolate and the ones infected with WADHc isolate had mean PPP of 19.63 ± 0.26 and 19.50 ± 0.19, respectively. Goats infected with WADHc isolate had significantly higher FEC (P = 0.004) and WB (P = 0.001). BW were significantly higher (P = 0.004) both in the controls and in Group 2 goats infected with WADHc isolate than in Group 1 goats infected with the RSHc isolate. BCS of animals in both infected groups dropped significantly (P = 0.001). There was a significant drop in PCV (P = 0.004) of both infected groups in comparison. Both isolates of H. contortus were pathogenic to the host.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2015 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2015-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/728210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65123420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

No Polymorphism in Plasmodium falciparum K13 Propeller Gene in Clinical Isolates from Kolkata, India 印度加尔各答临床分离株恶性疟原虫K13螺旋桨基因无多态性

IF 2.6

Journal of Pathogens Pub Date : 2015-11-25 DOI: 10.1155/2015/374354

Moytrey Chatterjee, S. Ganguly, Pabitra Saha, B. Bankura, N. Basu, M. Das, S. Guha, A. Maji

引用次数: 21

Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran 质粒介导的喹诺酮类耐药基因在伊朗产β-内酰胺酶广谱肺炎克雷伯菌分离株中的流行

IF 2.6

Journal of Pathogens Pub Date : 2015-11-04 DOI: 10.1155/2015/434391

Ehsaneh Shams, F. Firoozeh, R. Moniri, M. Zibaei

{"title":"Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran","authors":"Ehsaneh Shams, F. Firoozeh, R. Moniri, M. Zibaei","doi":"10.1155/2015/434391","DOIUrl":"https://doi.org/10.1155/2015/434391","url":null,"abstract":"The purpose of this study was to determine the prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA) among ESBL-producing Klebsiella pneumoniae isolates in Kashan, Iran. A total of 185 K. pneumoniae isolates were tested for quinolone resistance and ESBL-producing using the disk diffusion method and double disk synergy (DDST) confirmatory test. ESBL-producing strains were further evaluated for the bla CTX-M genes. The PCR method was used to show presence of plasmid-mediated quinolone resistance genes and the purified PCR products were sequenced. Eighty-seven ESBL-producing strains were identified by DDST confirmatory test and majority (70, 80.5%) of which carried bla CTX-M genes including CTX-M-1 (60%), CTX-M-2 (42.9%), and CTX-M-9 (34.3%). Seventy-seven ESBL-producing K. pneumoniae isolates harbored PMQR genes, which mostly consisted of aac(6′)-Ib-cr (70.1%) and qnrB (46.0%), followed by qnrS (5.7%). Among the 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried 2 and 3 different PMQR genes, respectively. However, qnrA and qepA were not found in any isolate. Our results highlight high ESBL occurrence with CTX-M type and high frequency of plasmid-mediated quinolone resistance genes among ESBL-producing K. pneumoniae isolates in Kashan.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2015 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2015-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/434391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64964033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

General Overview on Nontuberculous Mycobacteria, Biofilms, and Human Infection 非结核分枝杆菌、生物膜和人类感染的概述

IF 2.6

Journal of Pathogens Pub Date : 2015-11-04 DOI: 10.1155/2015/809014

S. Faria, Inês João, L. Jordão

引用次数: 115

Human Papillomavirus Infection, Infertility, and Assisted Reproductive Outcomes 人乳头瘤病毒感染、不孕症和辅助生殖结果

IF 2.6

Journal of Pathogens Pub Date : 2015-11-01 DOI: 10.1155/2015/578423

N. Pereira, Katherine M. Kucharczyk, Jaclyn L. Estes, Rachel S. Gerber, J. Lekovich, R. Elias, S. Spandorfer

{"title":"Human Papillomavirus Infection, Infertility, and Assisted Reproductive Outcomes","authors":"N. Pereira, Katherine M. Kucharczyk, Jaclyn L. Estes, Rachel S. Gerber, J. Lekovich, R. Elias, S. Spandorfer","doi":"10.1155/2015/578423","DOIUrl":"https://doi.org/10.1155/2015/578423","url":null,"abstract":"The human papillomavirus (HPV) is a sexually transmitted infection common among men and women across all geographic and socioeconomic subgroups worldwide. Recent evidence suggests that HPV infection may affect fertility and alter the efficacy of assisted reproductive technologies. In men, HPV infection can affect sperm parameters, specifically motility. HPV-infected sperm can transmit viral DNA to oocytes, which may be expressed in the developing blastocyst. HPV can increase trophoblastic apoptosis and reduce the endometrial implantation of trophoblastic cells, thus increasing the theoretical risk of miscarriage. Vertical transmission of HPV during pregnancy may be involved in the pathophysiology of preterm rupture of membranes and spontaneous preterm birth. In patients undergoing intrauterine insemination for idiopathic infertility, HPV infection confers a lower pregnancy rate. In contrast, the evidence regarding any detrimental impact of HPV infection on IVF outcomes is inconclusive. It has been suggested that vaccination could potentially counter HPV-related sperm impairment, trophoblastic apoptosis, and spontaneous miscarriages; however, these conclusions are based on in vitro studies rather than large-scale epidemiological studies. Improvement in the understanding of HPV sperm infection mechanisms and HPV transmission into the oocyte and developing blastocyst may help explain idiopathic causes of infertility and miscarriage.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"23 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/578423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65038898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 51

Protective Effects of Tinospora crispa Stem Extract on Renal Damage and Hemolysis during Plasmodium berghei Infection in Mice crispa茎提取物对伯氏疟原虫感染小鼠肾损伤和溶血的保护作用

IF 2.6

Journal of Pathogens Pub Date : 2015-10-27 DOI: 10.1155/2015/738608

Narain Nutham, Sakuna Sakulmettatham, Suwit Klongthalay, P. Chutoam, Voravuth Somsak

{"title":"Protective Effects of Tinospora crispa Stem Extract on Renal Damage and Hemolysis during Plasmodium berghei Infection in Mice","authors":"Narain Nutham, Sakuna Sakulmettatham, Suwit Klongthalay, P. Chutoam, Voravuth Somsak","doi":"10.1155/2015/738608","DOIUrl":"https://doi.org/10.1155/2015/738608","url":null,"abstract":"Renal damage and hemolysis induced by malaria are associated with mortality in adult patients. It has been speculated that oxidative stress condition induced by malaria infection is involved in its pathology. Thus, we aimed to investigate the protective effects of Tinospora crispa stem extract on renal damage and hemolysis during Plasmodium berghei infection. T. crispa stem extract was prepared using hot water method and used for oral treatment in mice. Groups of ICR mice were infected with 1 × 107 parasitized erythrocytes of P. berghei ANKA by intraperitoneal injection and given the extracts (500, 1000, and 2000 mg/kg) twice a day for 4 consecutive days. To assess renal damage and hemolysis, blood urea nitrogen (BUN), creatinine, and hematocrit (%Hct) levels were then evaluated, respectively. Malaria infection resulted in renal damage and hemolysis as indicated by increasing of BUN and creatinine and decreasing of %Hct, respectively. However, protective effects on renal damage and hemolysis were observed in infected mice treated with these extracts at doses of 1000 and 2000 mg/kg. In conclusion, T. crispa stem extract exerted protective effects on renal damage and hemolysis induced by malaria infection. This plant may work as potential source in the development of variety of herbal formulations for malarial treatment.","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2015 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2015-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/738608","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65130045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria. 薄荷精油对常见食源性致病菌的化学成分及体外抗菌活性研究

IF 2.6

Journal of Pathogens Pub Date : 2015-01-01 Epub Date: 2015-08-16 DOI: 10.1155/2015/916305

Yasser Shahbazi

{"title":"Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria.","authors":"Yasser Shahbazi","doi":"10.1155/2015/916305","DOIUrl":"https://doi.org/10.1155/2015/916305","url":null,"abstract":"The aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of Mentha spicata plant against common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157:H7). Chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (GC-MS). The antibacterial activity of the essential oil was evaluated by broth microdilution method and agar disk diffusion assay. According to the result of GC-MS analysis, 18 components were identified, accounting for 99.89% of the whole essential oil. The main components were carvone (78.76%), limonene (11.50%), β-bourbonene (11.23%), cis-dihydrocarveol (1.43%), trans-caryophyllene (1.04%), menthone (1.01%), menthol (1%), and terpinen-4-ol (0.99). The essential oil exhibited moderate level of antibacterial activity against all test microorganisms. In general, Gram-positive bacteria were more susceptible to M. spicata essential oil than Gram-negative bacteria. L. monocytogenes was the most sensitive of the microorganisms to the antibacterial activity of M. spicata essential oil (inhibition zone = 22 mm and MIC and MBC = 2.5 µL/mL). Based on our results, the essential oil of M. spicata plant collected from Kermanshah province, west of Iran, has a potential to be applied as antibacterial agent. ","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2015 ","pages":"916305"},"PeriodicalIF":2.6,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/916305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33988727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 84

Prevalence of Virulence Factors and Drug Resistance in Clinical Isolates of Enterococci: A Study from North India. 印度北部肠球菌临床分离株毒力因子和耐药性的流行病学研究。

IF 2.6

Journal of Pathogens Pub Date : 2015-01-01 Epub Date: 2015-08-23 DOI: 10.1155/2015/692612

Tuhina Banerjee, Shampa Anupurba

{"title":"Prevalence of Virulence Factors and Drug Resistance in Clinical Isolates of Enterococci: A Study from North India.","authors":"Tuhina Banerjee, Shampa Anupurba","doi":"10.1155/2015/692612","DOIUrl":"https://doi.org/10.1155/2015/692612","url":null,"abstract":"Along with emergence of multidrug resistance, presence of several virulence factors in enterococci is an emerging concept. This study was undertaken to determine the prevalence of various virulence factors phenotypically and genotypically in enterococci and study their association with multidrug resistance. A total of 310 enterococcal isolates were studied, comprising 155 E. faecium and 155 E. faecalis. Antimicrobial susceptibility testing was done by disc diffusion and agar dilution method. Hemolysin, gelatinase, biofilm production, and haemagglutination were detected phenotypically and presence of virulence genes, namely, asa1, gelE, cylA, esp, and hyl, was detected by multiplex PCR. Of the total, 47.41% isolates were high level gentamicin resistant (HLGRE) and 7.09% were vancomycin resistant (VRE). All the virulence traits studied were found in varying proportions, with majority in E. faecalis (p > 0.05). Strong biofilm producers possessed either asa1 or gelE gene. gelE silent gene was detected in 41.37% (12/29). However, increase in resistance was associated with significant decrease in expression or acquisition of virulence genes. Further, acquisition of vancomycin resistance was the significant factor responsible for the loss of virulence traits. Though it is presumed that increased drug resistance correlates with increased virulence, acquisition of vancomycin resistance might be responsible for reduced expression of virulence traits to meet the \"biological cost\" relating to VRE. ","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2015 ","pages":"692612"},"PeriodicalIF":2.6,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/692612","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34068309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

An Additive Effect of Oral N-Acetyl Cysteine on Eradication of Helicobacter pylori. 口服n -乙酰半胱氨酸对根除幽门螺杆菌的加性作用。

IF 2.6

Journal of Pathogens Pub Date : 2015-01-01 Epub Date: 2015-09-02 DOI: 10.1155/2015/540271

Seyed Mohammad-Taghi Hamidian, Najmeh-Sadat Aletaha, Reza Taslimi, Mohammad Montazeri

{"title":"An Additive Effect of Oral N-Acetyl Cysteine on Eradication of Helicobacter pylori.","authors":"Seyed Mohammad-Taghi Hamidian, Najmeh-Sadat Aletaha, Reza Taslimi, Mohammad Montazeri","doi":"10.1155/2015/540271","DOIUrl":"https://doi.org/10.1155/2015/540271","url":null,"abstract":"Background. Helicobacter pylori is highly adapted to the gastric environment where it lives within or beneath the gastric mucous layer. The aim of this study was to evaluate whether the addition of N-acetyl cysteine to the treatment regimen of H. pylori infection would affect eradication rates of the disease. Methods. A total of 79 H. pylori positive patients were randomized to two therapeutic groups. Both groups received a 14-day course of three-drug regimen including amoxicillin/clarithromycin/omeprazole. Experimental group (38 subjects) received NAC, and control group (41 subjects) received placebo, besides three-drug regimen. H. pylori eradication was evaluated by urea breath test at least 4 weeks after the cessation of therapy. Results. The rate of H. pylori eradication was 72.9% and 60.9% in experimental and control groups, respectively (P = 0.005). By logistic regression modeling, female gender (OR 3.68, 95% CI: 1.06-5.79; P = 0.040) and treatment including NAC (OR 1.88, 95% CI: 0.68-3.15; P = 0.021) were independent factors associated with H. pylori eradication. Conclusion. The results of the present study show that NAC has an additive effect on the eradication rates of H. pylori obtained with three-drug regimen and appears to be a promising means of eradicating H. pylori infection. ","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2015 ","pages":"540271"},"PeriodicalIF":2.6,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/540271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34048554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Detection of Legionella spp. from Domestic Water in the Prefecture of Arta, Greece. 希腊阿尔塔省生活用水中军团菌的检测。

IF 2.6

Journal of Pathogens Pub Date : 2014-01-01 Epub Date: 2014-03-12 DOI: 10.1155/2014/407385

Dimitra Dimitriadi, Emmanuel Velonakis

{"title":"Detection of Legionella spp. from Domestic Water in the Prefecture of Arta, Greece.","authors":"Dimitra Dimitriadi, Emmanuel Velonakis","doi":"10.1155/2014/407385","DOIUrl":"https://doi.org/10.1155/2014/407385","url":null,"abstract":"The aim of this research was the isolation of Legionella spp. from domestic water supply networks in the Prefecture of Arta. A total of 100 water samples, from 25 houses, were collected. Half of the samples concerned the cold water and half the hot water supply. Purpose was to detect colonization of the water networks with Legionella spp. >500 cfu/L by using the method of filtration (ISO 11731). Out of 100 samples, 6 samples from 3 houses were positive for Legionella spp. Legionella pneumophila serogroup 2-14 was isolated in 5 of 6 samples, whereas in the sixth sample Legionella anisa was identified. Only three of the samples had residual chloride over 0.2 mg/L, rate which is necessary for potable water, according to the Greek hygienic practice. Concerning the temperature of hot water, the mean temperature of the negative for Legionella samples was higher compared to the mean temperature of the positive for Legionella samples (49.9°C versus 45.5°C). It is estimated that there is risk of infection through the use of showers. The low concentration of chloride and the temperature, which was found within the limits favorable to developing Legionella spp. (20-45°C), provide fertile ground for proliferation of the bacteria. ","PeriodicalId":16788,"journal":{"name":"Journal of Pathogens","volume":"2014 ","pages":"407385"},"PeriodicalIF":2.6,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/407385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32272951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14