Susceptibility Pattern and Distribution of Oxacillinases and bla PER-1 Genes among Multidrug Resistant Acinetobacter baumannii in a Teaching Hospital in Iran

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2015-12-31 DOI:10.1155/2015/957259

Sareh Bagheri Josheghani, R. Moniri, F. Firoozeh, M. Sehat, Yasaman Dasteh Goli

{"title":"Susceptibility Pattern and Distribution of Oxacillinases and bla PER-1 Genes among Multidrug Resistant Acinetobacter baumannii in a Teaching Hospital in Iran","authors":"Sareh Bagheri Josheghani, R. Moniri, F. Firoozeh, M. Sehat, Yasaman Dasteh Goli","doi":"10.1155/2015/957259","DOIUrl":null,"url":null,"abstract":"Acinetobacter baumannii (A. baumannii) is an important nosocomial pathogen in healthcare institutions. β-Lactamase-mediated resistance is the most common mechanism for carbapenem resistance in A. baumannii. The aim of this study was to determine the antibiotic resistance pattern, to detect OXA encoding genes, class A, bla PER-1, and to detect the presence of ISAba1. A total of 124 A. baumannii isolates were collected from hospitalized patients in a teaching hospital in Kashan, Iran. The susceptibility of isolates to different antibiotics was determined by disk-diffusion method. PCR was used to detect bla PER-1, bla OXA-23, bla OXA-24, bla OXA-51, bla OXA-58, and ISAba1 genes. All isolates were resistant to ceftazidime, ceftriaxone, and cefotaxime. All of the isolates revealed susceptibility to polymyxin B and colistin. Ninety-six percent of the isolates were extensive drug resistance (XDR), 5.6% extended spectrum beta-lactamase (ESBL), and 54.8% metallo-beta-lactamase (MBL). All isolates were positive for bla OXA-51 and ISAba1. bla OXA-23, bla OXA-24, and bla OXA-58 were found in 79.8%, 25%, and 3.2%, respectively. The frequency rate of bla PER-1 gene was 52.4%. Multidrug resistant A. baumannii isolates are increasing in our setting and extensively limit therapeutic options. The high rate presence of class D carbapenemase-encoding genes, mainly bla OXA-23 carbapenemases, is worrying and alarming as an emerging threat in our hospital.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/957259","citationCount":"22","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2015/957259","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 22

Abstract

Acinetobacter baumannii (A. baumannii) is an important nosocomial pathogen in healthcare institutions. β-Lactamase-mediated resistance is the most common mechanism for carbapenem resistance in A. baumannii. The aim of this study was to determine the antibiotic resistance pattern, to detect OXA encoding genes, class A, bla PER-1, and to detect the presence of ISAba1. A total of 124 A. baumannii isolates were collected from hospitalized patients in a teaching hospital in Kashan, Iran. The susceptibility of isolates to different antibiotics was determined by disk-diffusion method. PCR was used to detect bla PER-1, bla OXA-23, bla OXA-24, bla OXA-51, bla OXA-58, and ISAba1 genes. All isolates were resistant to ceftazidime, ceftriaxone, and cefotaxime. All of the isolates revealed susceptibility to polymyxin B and colistin. Ninety-six percent of the isolates were extensive drug resistance (XDR), 5.6% extended spectrum beta-lactamase (ESBL), and 54.8% metallo-beta-lactamase (MBL). All isolates were positive for bla OXA-51 and ISAba1. bla OXA-23, bla OXA-24, and bla OXA-58 were found in 79.8%, 25%, and 3.2%, respectively. The frequency rate of bla PER-1 gene was 52.4%. Multidrug resistant A. baumannii isolates are increasing in our setting and extensively limit therapeutic options. The high rate presence of class D carbapenemase-encoding genes, mainly bla OXA-23 carbapenemases, is worrying and alarming as an emerging threat in our hospital.

查看原文本刊更多论文

伊朗某教学医院耐多药鲍曼不动杆菌Oxacillinases和bla PER-1基因的易感模式及分布

鲍曼不动杆菌(鲍曼不动杆菌)是卫生保健机构中重要的医院病原体。β-内酰胺酶介导的耐药是鲍曼不动杆菌对碳青霉烯类耐药最常见的机制。本研究的目的是确定抗生素耐药模式，检测OXA编码基因，A类，bla PER-1，以及检测ISAba1的存在。从伊朗卡尚一教学医院住院患者中共收集到124株鲍曼不动杆菌分离株。采用纸片扩散法测定菌株对不同抗生素的药敏。PCR检测bla PER-1、bla OXA-23、bla OXA-24、bla OXA-51、bla OXA-58和ISAba1基因。所有分离株均对头孢他啶、头孢曲松和头孢噻肟耐药。所有分离株均对多粘菌素B和粘菌素敏感。广泛耐药(XDR)占96%，扩展谱β -内酰胺酶(ESBL)占5.6%，金属β -内酰胺酶(MBL)占54.8%。所有分离株均为bla OXA-51和ISAba1阳性。bla OXA-23、bla OXA-24和bla OXA-58分别占79.8%、25%和3.2%。bla PER-1基因检出率为52.4%。在我们的环境中，耐多药鲍曼不动杆菌分离株正在增加，并广泛限制了治疗选择。D类碳青霉烯酶编码基因，主要是bla OXA-23碳青霉烯酶，作为一种新出现的威胁，在我院的高发率令人担忧和警惕。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.