Journal of oral sciencePub Date : 2023-01-11Epub Date: 2022-12-16DOI: 10.2334/josnusd.22-0235
Iris Carrillo-Novia, Edith Lara-Carrillo, Olivia Torres-Bugarin, Adriana A Morales-Valenzuela, Elías N Salmerón-Valdés, Wael Hegazy-Hassan, Ulises Velázquez-Enríquez, Víctor H Toral-Rizo
{"title":"Use of liquid-based cytology samples reveals genomic instability and cell death in patients undergoing orthodontic treatment.","authors":"Iris Carrillo-Novia, Edith Lara-Carrillo, Olivia Torres-Bugarin, Adriana A Morales-Valenzuela, Elías N Salmerón-Valdés, Wael Hegazy-Hassan, Ulises Velázquez-Enríquez, Víctor H Toral-Rizo","doi":"10.2334/josnusd.22-0235","DOIUrl":"10.2334/josnusd.22-0235","url":null,"abstract":"<p><strong>Purpose: </strong>To examine the use of liquid-based exfoliative cytology to determine the presence of genomic instability and cell death in the oral mucosa of patients with orthodontic appliances.</p><p><strong>Methods: </strong>Fifty-four oral mucosa samples were collected from 18 patients and divided into three stages: T0, before fixation of orthodontic appliances; T1, 25 days after appliance fixation; T2, 90 days after appliance fixation. All samples were Papanicolaou-stained and observed by microscopy (1,000 cells/sample) to ascertain the frequency of micronucleated cells (MN) and nuclear abnormalities (nuclear buds (NB), binucleated (BN), condensed chromatin (CC), karyorrhexis (KR), and karyolysis (KL)). Differences were analyzed statistically using the Mann-Whitney, Wilcoxon, Kruskal-Wallis and chi-squared tests.</p><p><strong>Results: </strong>After placement of orthodontic appliances, significant differences were observed for genomic instability biomarkers (MN and NB) and cell death (CC, KR and KL) (P < 0.05). Female patients and older patients exhibited a higher frequency of MN.</p><p><strong>Conclusion: </strong>Liquid-based cytology has revealed that orthodontic appliances induce genomic instability and cell death in epithelial tissue of the oral mucosa, facilitating sample preservation and yielding more than one preparation per sample. Future studies should investigate whether such cell damage can be reversed through cell repair or whether cell alterations evolve and lead to disease.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10545789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zehra Süsgün Yıldırım, Ersan Eyiler, Zeliha G Bek Kürklü
{"title":"Effect of thickness on the degree of conversion, monomer elution, depth of cure and cytotoxicity of bulk-fill composites.","authors":"Zehra Süsgün Yıldırım, Ersan Eyiler, Zeliha G Bek Kürklü","doi":"10.2334/josnusd.22-0374","DOIUrl":"https://doi.org/10.2334/josnusd.22-0374","url":null,"abstract":"<p><strong>Purpose: </strong>Today, bulk-fill composites are used as a single layer with a thickness of up to 4-5 mm. However, is proper polymerization achieved with this increased thickness?</p><p><strong>Methods: </strong>This study was designed to investigate the effect of thickness on the degree of conversion (DC) (n = 6), the elution of monomers (n = 6), depth of cure (DoC) (n = 10) and cytotoxicity (n = 6) of the bulk-fill composites SDR Flow Plus (SDR), SonicFill2 SingleFill (SF) and ACTIVA Bioactive Restorative (ACT) in comparison to the conventional G-aenial Posterior (GC). Two-way analysis of variance (ANOVA) was used to assess the interaction between materials and surfaces, and one-way ANOVA and Tukey tests were used to compare the degree of conversion, monomer elution and cytotoxicity values (P < 0.05).</p><p><strong>Results: </strong>The highest DC was found at the top surface of SDR, while the lowest DC was found at SF. The V2 mm/V0 mm DoC ratios of the composites except ACTs were appropriate according to the threshold. None of the composites were cytotoxic on day 1.</p><p><strong>Conclusion: </strong>In bulk-fill composites, DC decreased and monomer elution increased with increasing depth. The V4 mm/V0 mm ratios of all bulk-fill groups were not appropriate. Additionally, only ACTs had a cell viability of <70% on day 7.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9219012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Başak Yazkan, Ebru Yilmaz, Özge Gizem Yenidünya, Nilgün Akgül
{"title":"Effects of different toothpastes on the color and whiteness of stained anterior composite resin.","authors":"Başak Yazkan, Ebru Yilmaz, Özge Gizem Yenidünya, Nilgün Akgül","doi":"10.2334/josnusd.23-0106","DOIUrl":"10.2334/josnusd.23-0106","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate changes in the color of an anterior nanohybrid composite resin and differences in its whitening index after 30 days of brushing with six brands of whitening toothpaste in vitro to simulate the situation in individuals with high coffee consumption.</p><p><strong>Methods: </strong>Eighty-four disk-shaped resin specimens were prepared (shade A2). For initial staining, the samples were immersed in coffee solution for 14 days and then divided into eight groups for treatment with various toothpastes. Color was assessed at the baseline, after initial staining, and on days 7, 14, and 30 of the staining-and-brushing cycle. Color stability was evaluated based on the CIEDE2000 color difference (ΔE<sub>00</sub>), and differences in whiteness were determined using the whitening index for dentistry (WID).</p><p><strong>Results: </strong>The highest ΔE<sub>00</sub> and ΔWID values were obtained with Colgate Herbal, followed by Opalescence Whitening and Crest Baking Soda and Peroxide, whereas the lowest values were obtained with distilled water, followed by Yotuel Pharma B5, on day 30 of the staining-and-brushing cycle.</p><p><strong>Conclusion: </strong>In terms of improving the color and whiteness of coffee-stained anterior nanohybrid composite resin, Colgate Herbal, Opalescence Whitening, and Crest Baking Soda and Peroxide yielded promising results after 30 days of continuous use.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41125687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Association of second-hand smoke exposure, quantified by salivary cotinine, with dental caries in Japanese adolescents.","authors":"Naohiro Takao, Michiko Furuta, Toru Takeshita, Shinya Kageyama, Takuro Goto, Myrna N Zakaria, Kenji Takeuchi, Yoshihisa Yamashita","doi":"10.2334/josnusd.22-0388","DOIUrl":"https://doi.org/10.2334/josnusd.22-0388","url":null,"abstract":"<p><strong>Purpose: </strong>Second-hand smoke has adverse effects on oral health. This cohort study used a multilevel approach to investigate the association of second-hand smoke exposure, as determined by salivary cotinine level, with dental caries in adolescents.</p><p><strong>Methods: </strong>Data from 75 adolescents aged 11 or 12 years and 2,061 teeth without dental caries were analyzed in this study. Annual dental examinations to assess dental caries were conducted between 2018 and 2021. Salivary cotinine and Dentocult SM-Strip level were measured at baseline. Information on the smoking habits of parents, snack frequency, regular dental visits, and use of fluoride toothpaste was collected at baseline from parent-reported questionnaires.</p><p><strong>Results: </strong>During the 3-year follow-up, dental caries was noted in 21 adolescents and 43 teeth. Participants exposed to parental smoking had higher salivary cotinine levels than those whose parents did not smoke. The multilevel Cox regression model showed that a high salivary cotinine level was associated with the incidence of dental caries, after adjusting for potential confounding factors (hazard ratio, 3.39; 95% confidence interval 1.08-10.69).</p><p><strong>Conclusion: </strong>This study suggests that the risk of dental caries is higher for adolescents who have high salivary cotinine levels attributable to second-hand smoke exposure.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9219013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transient receptor potential canonical 1 is a candidate treatment target for tongue squamous cell carcinoma by inhibiting growth and invasion through phosphatidylinositol 3-kinase and protein kinase B pathway.","authors":"Bing Zhou, Lei Jiang","doi":"10.2334/josnusd.22-0376","DOIUrl":"https://doi.org/10.2334/josnusd.22-0376","url":null,"abstract":"<p><strong>Purpose: </strong>Transient receptor potential canonical 1 (TRPC1) modulates tumor growth and invasion, however, its role in tongue squamous cell carcinoma (TSCC) is unclear. The aim of this study was to explore the effect of TRPC1 knockdown on cellular function and its underlying molecular mechanism in TSCC.</p><p><strong>Methods: </strong>TSCC cell lines were transfected with TRPC1 or negative control small interfering ribonucleic acids, and then PI3K activator was incubated after transfection.</p><p><strong>Results: </strong>TRPC1 was elevated in TSCC cell lines (including SCC-15, CAL-33, HSC-3, and YD-15) compared to control cells (all P < 0.05). Since TRPC1 was clearly increased in SCC-15 and YD-15 cells, they were selected for further study. In both YD-15 and SCC-15 cells, TRPC1 knockdown decreased cell proliferation at 48 h and 72 h (all P < 0.05), increased apoptosis (both P < 0.05), and declined invasion (both P < 0.05). Meanwhile, TRPC1 knockdown decreased phosphatidylinositol 3-kinase and protein kinase B phosphorylation (all P < 0.05). Additionally, the effect of TRPC1 knockdown on cell proliferation at 48 h and 72 h, apoptosis, and invasion was attenuated by PI3K activator (all P < 0.05).</p><p><strong>Conclusion: </strong>TRPC1 shows potential as a candidate treatment target, whose knockdown inhibits growth and invasion through inactivating PI3K/AKT pathway in TSCC.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9587138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of the effects of apical backfilling depth on apical sealing of different root canal filling qualities and morphologies.","authors":"Qian-Nan Zhang, Yu Zhang, Chen-Chen Zhang, Yue Yuan, Qi Wang, Wei-Dong Yang","doi":"10.2334/josnusd.22-0346","DOIUrl":"https://doi.org/10.2334/josnusd.22-0346","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to evaluate the effects of apical backfilling depth on the apical sealing of different root canal filling qualities and morphologies.</p><p><strong>Methods: </strong>3D-printed root canals (A: round, B: oval, C: long oval, D: flat) were used and divided into subgroups by root canal filling quality (a: good, b: poor, c: nonfilling) and backfilling depth (3 mm, 5 mm). A glucose microleakage device was used to measure leakage.</p><p><strong>Results: </strong>(1) 3-mm iRoot BP Plus was filled at the apex, and no obvious leakage occurred in the good root canal filling group, which was significantly smaller than that in the poor/nonfilling groups (P < 0.05). Under good root canal filling conditions in groups A, B, C, and D, no obvious leakage was observed. Under poor/nonfilling root canal filling conditions, there was significant leakage; A and B (P > 0.05) and C and D were compared (P < 0.05). (2) Apical backfilling with 5-mm iRoot BP Plus showed no significant leakage in the poor root canal filling groups with the four morphologies.</p><p><strong>Conclusion: </strong>3-mm iRoot BP Plus was filled at the apex, root canal filling was poor, apical sealing was poor, and root canal morphology affected apical sealing. Apical backfilling with 5-mm iRoot BP Plus improved apical sealing under poor root canal filling conditions, and apical sealing was unaffected by root canal morphology.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10303926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A case of sclerosing odontogenic carcinoma of the mandible with a review of the literature.","authors":"Naoya Kinoshita, Hirofumi Tomioka, Yu Oikawa, Yuki Fukawa, Tohru Ikeda, Hiroyuki Harada","doi":"10.2334/josnusd.23-0187","DOIUrl":"10.2334/josnusd.23-0187","url":null,"abstract":"<p><p>Sclerosing odontogenic carcinoma (SOC) is a very rare malignant odontogenic tumor characterized by sclerotic stroma and single-file cord-like tumor cell structures. A 38-year-old man presented with extraoral swelling and right mental region paralysis. Panoramic radiography revealed an ill-defined radiolucent lesion extending from the right mandibular ramus to the right lower canine. Magnetic resonance imaging showed tumor invasion into the right inferior alveolar nerve and masseter muscle. Hemimandibulectomy, bilateral neck dissection, and mandibular reconstruction were performed using a rectus abdominis musculocutaneous flap and a titanium plate. Histopathology and immunohistochemistry confirmed SOC diagnosis. No recurrence occurred in the 1-year follow-up. In this paper, a case of SOC with a high Ki-67 labeling index was reported. Since SOC is prone to nerve invasion, treatment is resection with an appropriate surgical margin.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41128493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The bactericidal and biofilm removal effect of super reducing water on Streptococcus mutans in three types of orthodontic brackets.","authors":"Hajime Shiiki, Sho Goto, Hiroaki Katsuragi, Sakurako Kobayashi","doi":"10.2334/josnusd.22-0320","DOIUrl":"https://doi.org/10.2334/josnusd.22-0320","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the bactericidal and biofilm removal effect of super reducing water (SRW) on Streptococcus mutans (S. mutans) adhered to orthodontic brackets, in vitro.</p><p><strong>Methods: </strong>Three types of brackets were bonded to aluminum disks. After the formation of S. mutans biofilms on the surfaces, the brackets were divided into three groups (n = 44 each) based on their exposure to SRW: group 1, no treatment; group 2, treated for 5 min; and group 3, treated for 10 min. Total viable counts, adenosine triphosphate measurements, crystal violet assay, and scanning electron microscopy were used to evaluate the effect of SRW.</p><p><strong>Results: </strong>The bacterial counts in groups 2 and 3 were significantly lower than those in group 1 (P < 0.001); however, no significant differences were observed between groups 2 and 3. Marked decreases in the number of bacterial colonies and extent of biofilm formation were observed in groups 2 and 3 compared to group 1. No significant differences in the number of bacterial colonies and amount of biofilm were observed among the three types of brackets in each group.</p><p><strong>Conclusion: </strong>These findings indicate the bactericidal and biofilm removal effect of SRW treatment on S. mutans adhered to orthodontic brackets.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9072180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of reaction products on hydroxyapatite created by tooth etchants with different compositions.","authors":"Takaaki Sato, Jirun Sun, Xiaohong Wang, Shozo Takagi, Yoshikazu Teranishi, Go Inoue, Tomohiro Takagaki, Toru Nikaido, Junji Tagami, Yasushi Shimada","doi":"10.2334/josnusd.22-0268","DOIUrl":"https://doi.org/10.2334/josnusd.22-0268","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to investigate the reaction products formed by application of three tooth etchants to hydroxyapatite.</p><p><strong>Methods: </strong>Tooth etchants with three different compositions, designed for application to teeth before dental adhesive - \" K-etchant GEL\" (containing phosphoric acid), \"Enamel Conditioner\" (containing organic acids), and \"Multi Etchant\" (containing acidic monomer) - were applied to hydroxyapatite plates.</p><p><strong>Results: </strong>Atomic force microscopy measurements revealed that Multi Etchant formed nano-sized particles on the hydroxyapatite. X-ray diffraction and Fourier transform infrared spectrometer analyses of the powdered hydroxyapatite indicated that Enamel Conditioner produced calcium tartrate whereas K-etchant GEL generated monetite. These results indicated that each etchant reacted with hydroxyapatite in a different way.</p><p><strong>Conclusion: </strong>Not only differences among the etching ability of etchants, but also differences in the reaction compounds they produce may influence bonding performance in clinical practice.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9072181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peng Yu, Hao Luo, Adrian U Yap, Fu-Cong Tian, Xiao-Yan Wang
{"title":"Effects of polishing press-on force on surface roughness and gloss of CAD-CAM composites.","authors":"Peng Yu, Hao Luo, Adrian U Yap, Fu-Cong Tian, Xiao-Yan Wang","doi":"10.2334/josnusd.22-0402","DOIUrl":"https://doi.org/10.2334/josnusd.22-0402","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to determine the effect of polishing press-on force on surface roughness and gloss of computer-aided design-computer-aided manufacturing (CAD-CAM) composites.</p><p><strong>Methods: </strong>The materials evaluated included a CAD-CAM ceramic, a polymer-infiltrated ceramic, and three filler-based CAD-CAM composites. The CAD-CAM blocks were sectioned, embedded in self-cured resin, finished with abrasive papers and ultrasonically cleaned. Specimens were subsequently polished using the Sof-Lex disk system with 0.5, 1.0, 1.5, and 2.0 N press-on force by means of a custom-made apparatus. Contour arithmetic mean deviation (Ra) and gloss value (GU) data were acquired with a profilometer and glossmeter, respectively, and analyzed using ANOVA/Bonferroni post hoc test and Pearson's correlation (α = 0.05). Representative samples of the various materials at baseline and after each polishing step were examined under scanning electron microscope.</p><p><strong>Results: </strong>Mean Ra and GU values ranged from 0.096 ± 0.004 μm to 0.295 ± 0.045 μm and 13.4 ± 1.9 to 67.6 ± 11.3 correspondingly for the various material-force combinations. Surface roughness and gloss were found to be press-on force and material dependent. A moderately strong and negative correlation (r<sub>s</sub> = -0.69) existed between Ra and GU values.</p><p><strong>Conclusion: </strong>For optimal smoothness and gloss, ceramic and polymer-infiltrated ceramic CAD-CAM materials must be polished with a 2.0 N force, while filler-based CAD-CAM composites should, in general, be polished using a 1.0 to 1.5 N force.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9232895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}