富含柠檬醛成分的白皮精油作为免疫反应和抗白色念珠菌添加剂用于引导骨再生的胶原膜。

IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Nohora C Rugeles-Páez, Wendy L Quintero, Elena E Stashenko, Liliana T García
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引用次数: 0

摘要

目的:研究以白皮精油(EO)中富含萜烯的成分与氯己定协同混合为基础的添加剂,优化引导骨再生-可吸收膜(胶原)的免疫应答性和抗白色念珠菌(C. albicans)的性能。方法:抗c。利用浮游培养和固定式培养评估各组白念珠菌活性(单独或与氯己定联合)。J774A。采用小鼠巨噬细胞法测定其细胞毒性和免疫应答效应。结果:添加富柠檬醛组分后,氯己定对白色念珠菌的抗浮游和抗固定化作用提高2 ~ 4倍。对巨噬细胞,该成分也显示出潜在的细胞保护作用,以对抗氯己定的毒性作用,最大限度地减少对细胞膜、线粒体膜电位和核完整性的损害。在胶原膜碎片上生长的巨噬细胞,受到柠檬醛部分(单独或与氯己定一起)的刺激,显示出成骨细胞因子TNF-α的释放显著增加,IL-4的增强。结论:该联合疗法有望开发出一种预防性或治疗性杀菌剂,可优化氯己定的药理特性(上皮耐受性和抗c。白色念珠菌在表面巩固),以及增强胶原膜的免疫反应特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Citral-rich fractions of Lippia alba essential oils as immunoresponsive and anti-Candida albicans additives for collagen membranes in guided bone regeneration.

Purpose: The aim of this study was to optimize the immunoresponsive and anti-Candida albicans (C. albicans) properties of guided bone regeneration-resorbable membranes (collagen) using additives based on synergistic mixtures of chlorhexidine with terpene-enriched fractions from Lippia alba essential oils (EO).

Methods: The anti-C. albicans activity of the fractions (individually or combined with chlorhexidine) was evaluated using planktonic and sessile cultures. J774A.1 murine macrophage cells were used to determine the cytotoxicity and immunoresponsive effects of the therapies.

Results: The anti-planktonic and anti-sessile performance of chlorhexidine on C. albicans was improved 2- to 4-fold by supplementation with citral-rich fractions. On macrophages, this fraction also exhibited a potentially cytoprotective action against the toxic effects of chlorhexidine, minimizing damage to the cell membrane, mitochondrial membrane potential, and nuclear integrity. Macrophages growing on collagen-membrane fragments and stimulated with the citral fraction (alone or with chlorhexidine) showed a significant increase in releasing the osteogenic cytokine TNF-α and enhancing the IL-4.

Conclusion: This combined therapy appears as a promising platform for the development of a prophylactic or therapeutic biocidal solution that can optimize the pharmacological characteristics of chlorhexidine (epithelium tolerance and anti-C. albicans consolidation on surfaces), as well as potentiating the immunoresponsive properties of collagen membranes.

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来源期刊
Journal of oral science
Journal of oral science DENTISTRY, ORAL SURGERY & MEDICINE-MATERIALS SCIENCE, BIOMATERIALS
CiteScore
3.80
自引率
0.00%
发文量
58
期刊介绍: Information not localized
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