Journal of Orthopaedic Surgery and Research最新文献

{"title":"Icariin promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by activating PI3K-AKT-UTX/EZH2 signaling in steroid-induced femoral head osteonecrosis.","authors":"Wei Ji, Guoqing Gong, Yuanhang Liu, Yan Liu, Jie Zhang, Qiang Li","doi":"10.1186/s13018-025-05697-0","DOIUrl":"10.1186/s13018-025-05697-0","url":null,"abstract":"<p><strong>Background: </strong>Differentiation of bone marrow mesenchymal stem cells (BMSCs) is pivotal in the pathogenesis of steroid-induced femoral head osteonecrosis. Icariin, an active ingredient in Epimedii herba, has the potential to regulate osteogenic differentiation of BMSCs. Nevertheless, the related mechanism is still unclear. The study aimed to explore whether icariin can affect osteogenic differentiation by activating PI3K/AKT signaling to alter UTX and EZH2 expression and thus regulating osteogenesis-related genes in BMSCs.</p><p><strong>Methods: </strong>BMSCs were collected from Sprague Dawley rats and identified by measuring the positive ratios of cell markers using flow cytometry. Cells were treated with 1 μmol/L dexamethasone (DEX) for 24 h with or without 0.1-10 μM of icariin treatment. Cell counting Kit-8 (CCK-8) assays and flow cytometry analyses were performed to measure cell viability and apoptosis. Western blotting was conducted for measurement of apoptotic markers, factors involved in the PI3K/AKT-UTX/EZH2 pathway, osteogenic markers, and adipogenesis-related factors. Alizarin red S staining and Oil-red O staining were performed to measure the effect of DEX, icariin, UTX overexpression, or EZH2 knockdown on osteogenic and adipogenic differentiation of BMSCs.</p><p><strong>Results: </strong>Icariin ameliorated DEX-induced rat BMSC injury. Icariin activated the PI3K/AKT signaling, thereby upregulating UTX and phosphorylated EZH2 levels while inhibiting EZH2 and H3K27me3 expression. Additionally, icariin promoted osteogenic differentiation and inhibited adipogenic differentiation of BMSCs. Importantly, overexpressing UTX or silencing EZH2 exerted similar effects on BMSC differentiation as icariin did.</p><p><strong>Conclusions: </strong>Icariin promotes osteogenic differentiation of DEX-treated BMSCs by activating PI3K/AKT signaling to upregulate UTX and inhibit EZH2, finally inducing H3K27me3 depletion.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"290"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of surgery for multipleligament knee injuries on patellar position and patellofemoral function: a retrospective study.","authors":"Yuanping Liao, Xiao Wang, Xiaotao Shi, Guorui Cao, Honglue Tan","doi":"10.1186/s13018-025-05652-z","DOIUrl":"10.1186/s13018-025-05652-z","url":null,"abstract":"<p><strong>Background: </strong>Patients who undergo reconstruction for multiligament knee injuries (MLKIs) often exhibit knee instability and poor overall knee function during postoperative follow-up. This may be related to the changes in patellar position and decline in patellofemoral function after surgery.</p><p><strong>Objective: </strong>To evaluate the outcomes following reconstruction of MLKIs through the assessment of: (1) changes in patellar height; (2) anatomical changes in patellofemoral alignment, such as tilt or displacement; and (3) functional outcomes of the patellofemoral joint.</p><p><strong>Methods: </strong>This retrospective study included 45 patients who underwent reconstruction for MLKIs at our hospital between November 2015 and September 2022, with complete data and meeting the inclusion criteria. These patients formed the case group. An additional 20 outpatients without ligament injuries or patellar dislocation were selected as the normal control group. Patellar height changes in the case group were assessed preoperatively and postoperatively using the Caton-Deschamps (CD) and Insall-Salvati (IS) indices on lateral X-rays. Magnetic resonance imaging (MRI) was used to measure patellofemoral alignment parameters in both groups, including the sulcus angle (SA), patellar tilt angle (PTA), lateral patellofemoral angle (LPA), congruence angle (CA), and patellofemoral index (PI), to evaluate patellofemoral positioning. Additionally, the Kujala score questionnaire was used to assess the stability function of the patellofemoral joint.</p><p><strong>Results: </strong>Preoperative patellar height in the case group, measured by the CD and IS indices, was (1.07 ± 0.10, 1.10 ± 0.09), showing a statistically significant difference when compared to postoperative measurements (0.96 ± 0.13, 1.05 ± 0.10) (P < 0.05). However, postoperative patellofemoral alignment parameters, including SA, PTA, LPA, CA, and PI, in the case group showed no statistically significant differences compared to the control group (P > 0.05). At the latest follow-up, the patellofemoral function score in the case group was (89.0 ± 5.3), which was not significantly different from the control group (91.0 ± 2.9) (P > 0.05).</p><p><strong>Conclusion: </strong>After reconstruction of MLKIs, patellar height decreased but remained within the normal range. Patellofemoral alignment was well-maintained, and patellofemoral function was maintained.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"291"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11916915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibroblast IRF7-mediated chondrocyte apoptosis affects the progression of collapse in steroid-induced osteonecrosis of the femoral head.","authors":"Han Longfei, Fang Weihua, Han Mingli, Zhuang Zhikun, He Mincong, Wei Qiushi","doi":"10.1186/s13018-025-05557-x","DOIUrl":"10.1186/s13018-025-05557-x","url":null,"abstract":"<p><strong>Purpose: </strong>The objective of this study was to identify potential genes implicated in the \"peri-collapse\" synovium of osteonecrosis of the femoral head through coding gene sequencing and to further clarify their specific mechanisms via in vitro experiments.</p><p><strong>Methods: </strong>Steroid-induced osteonecrosis of the femoral head (SIONFH) (n = 3), femoral neck fracture (FNF) (n = 3), and hip osteoarthritis (HOA) (n = 3) Synovial tissue of the hip joint was collected in total hip arthroplasty. A cellular model of SIONFH constructed from rat synovial fibroblasts by lipopolysaccharide intervention. Lentiviral technology was used to construct a model for fibroblast knockout of the Irf7 gene. HE was used to compare the characteristics of synovial tissue damage, and immunofluorescence and immunohistochemistry were used to compare the expression levels of VIM, IRF7, and IFNα. PCR, WB, and IF were used to examine Irf7 knockdown efficiency, chondrocyte proliferation (Col2a1, Aggrecan, Sox9), cartilage matrix degradation (Mmp13), and apoptosis (Bcl2, Bax, and Caspase3) expression under co-culture conditions. Crystalline violet staining was used to observe the migration rate of fibroblasts, and flow cytometry was used to detect the apoptosis level of chondrocytes under co-culture conditions.</p><p><strong>Results: </strong>Transcriptome sequencing of synovial tissue and fibroblasts ultimately screened for six differential genes, HOOK1, RNPC3, KCNA3, CD48, IRF7, SAMD9. Compared to FNF and HOA, synovial inflammatory cell recruitment and synovial hyperplasia were more pronounced in SIONFH. IF and IHC confirmed high expression of IRF7 and IFNα in the synovium of SIONFH. PCR and WB results suggested that fibroblasts highly expressed Irf7, Hook1, Rnpc3, Kcna3, Cd48, Samd9, Il-6, and Tnfα after lipopolysaccharide intervention, and the expression levels of Il-6 and Tnfα were significantly reduced after knockdown of Irf7 (P < 0.001). In the co-culture system, fibroblasts intervened with lipopolysaccharide significantly promoted chondrocyte apoptosis, the rate of cartilage matrix degradation, while inhibiting the level of chondrocyte proliferation, and this result was significantly reversed in Irf7 knockout fibroblasts. This was supported by flow cytometry results.</p><p><strong>Conclusions: </strong>IRF7, HOOK1, RNPC3, KCNA3, CD48, and SAMD9 as potential genes affecting the progression of SIONFH collapse. Irf7 mediates the fibroblast inflammatory response and affects the collapse process of SIONFH by influencing chondrocyte apoptosis. Thus, intervention in IRF7 holds promise as one of the key targets for reversing the collapse process of SIONFH.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"292"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for metachronous periprosthetic joint infection in patients with multiple prosthetic joints: a systematic review and meta-analysis.","authors":"Yi Li, Xiaolin Quan, Cheng Zhou, Xin Duan, Mao Nie, Haibo Si","doi":"10.1186/s13018-025-05694-3","DOIUrl":"10.1186/s13018-025-05694-3","url":null,"abstract":"<p><strong>Object: </strong>Although periprosthetic joint infection (PJI) can affect multiple joints simultaneously, most individuals with multiple joint involvement exhibit PJI in only one joint. Data regarding the metachronous PJI management for these patients are limited. This study aimed to explore the risk factors for metachronous PJI in patients with multiple prosthetic joints, thereby guiding and optimizing clinical practice.</p><p><strong>Methods: </strong>The MEDLINE, Web of Science, Cochrane Library, and EMBASE were searched for all clinical studies of metachronous PJI from inception until May 2024. The clinical studies on risk factors for metachronous PJI in patients with multiple prosthetic joints after experiencing a periprosthetic infection were collected, with two authors independently screening the literatures. Newcastle Ottawa scale was used as a quality assessment tool for the included studies, and the meta-analysis was conducted to evaluate the potential risk factors of metachronous PJI.</p><p><strong>Results: </strong>A total of 1,544 patients with PJI after multiple joint arthroplasties were reported in 9 studies, including 189 with metachronous PJI. The meta-analysis showed that methicillin-resistant staphylococcus aureus (MRSA; OR, 3.43; 95%CI, 1.71-6.88; p = 0.0005), rheumatoid arthritis (RA; OR, 2.38; 95%CI, 1.06-5.38; p = 0.04), history of steroid use (OR, 2.93; 95%CI, 1.58-5.43; p = 0.0007), and previous or ongoing non-periprosthetic infection (OR, 4.47; 95%CI, 1.45-13.82; p = 0.009) were identified as significant risk factors for metachronous PJI in patients with multiple prosthetic joints. However, there was no significant difference between the metachronous PJI group and non-metachronous group in terms of revision, age, diabetes, and gender.</p><p><strong>Conclusion: </strong>Patients with MRSA, RA, history of steroid use, previous or ongoing non-periprosthetic infection are at significantly higher risk for metachronous PJI. Further research is needed to optimize management strategies for preventing metachronous PJI in patients with multiple prostheses after a single joint PJI.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"293"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robot milling system integrated design and finite element analysis of custom femoral prostheses.","authors":"Qi Wu, Pengju Yue, Siyu Yin, Wang Liu, Zhenjie Li, Renjie He","doi":"10.1186/s13018-025-05647-w","DOIUrl":"10.1186/s13018-025-05647-w","url":null,"abstract":"<p><p>The long-term stability of cementless femoral prostheses is primarily affected by aseptic loosening, micromotion, and stress shielding, all of which are related to the force transfer of the prosthesis. These factors can compromise the osseointegration of the proximal prosthesis, leading to aseptic loosening within the femoral cavity. Due to the individual variability of the femur, the fit between the prosthesis and the femoral cavity during the design phase may differ from the fit achieved during the surgical procedure. Consequently, the force transfer of the prosthesis postoperatively may not align with the results obtained from finite element analysis conducted during the design phase, making it challenging to control the micromotion and stress shielding of the prosthesis. The design model of a custom femoral prosthesis is based on the CT reconstruction of the patient' femur. The fit of prosthesis within the femoral cavity during the design phase should match the fit during the surgical operation. Consequently, the results of finite element analysis conducted during the design phase can be used to control the force transfer of the prosthesis postoperatively. This approach helps to prevent improper micromotion and stress shielding of the proximal prosthesis, which can compromise the primary stability of the prosthesis within the femoral cavity, thereby facilitating the osseointegration of the proximal prosthesis.This paper proposes a novel technology that combines the design, finite element analysis, and manufacturing of custom prostheses. Specifically, a CAD/CAM/robot integration method is used to fabricate these prostheses. This innovative technology not only enhances the control of force transfer in custom prostheses but also reduces design and manufacture time while lowering costs. In conclusion, the finite element analysis of the custom prosthesis effectively manages force transfer, and the milling errors associated with the custom prosthesis are less than 1 mm.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"294"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis between the mDIXON-quant fat quantification parameters of the infrapatellar fat pad and the severity of knee osteoarthritis.","authors":"Bao-Gen Zhao, Yu-Jin Zhang, Meng Wang, Ning Wang, Yong Wang, Ting Gao, Li Zhang","doi":"10.1186/s13018-025-05687-2","DOIUrl":"10.1186/s13018-025-05687-2","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to explore the correlation between the proton density fat fraction (PDFF) of the infrapatellar fat pad and the severity of knee osteoarthritis (KOA), with a focus on identifying potential imaging biomarkers for KOA progression.</p><p><strong>Methods: </strong>Seventy patients with KOA and forty-four healthy controls (HCs) were prospectively recruited for conventional MR and mDIXON-Quant sequence scanning. The mDIXON-Quant technique was selected for its precision in fat quantification and ability to provide three-dimensional water-fat separation, offering superior accuracy in assessing fat content compared to traditional methods.The severity of KOA was assessed via the whole-organ magnetic resonance imaging score (WORMS). Intraclass correlation coefficients (ICCs) were calculated to assess interobserver agreement for the PDFF measurements. Differences in the PDFF between KOA patients and HCs were compared. Additionally, the correlations between the PDFF of the infrapatellar fat pad in KOA patients and scores of the characteristic areas included in the knee WORMS were analyzed.</p><p><strong>Results: </strong>The PDFF of the infrapatellar fat pad in KOA patients was significantly lower than in HCs. PDFF in KOA patients was negatively correlated with multiple aspects of total knee WORMS scores (e.g., articular cartilage integrity, etc.; r from - 0.94 to -0.25; P < 0.05), except for medial or lateral collateral ligament integrity (r = 0.27, P ≥ 0.05). Interobserver agreement was excellent (ICC = 0.793, P < 0.001).</p><p><strong>Conclusions: </strong>The PDFF of the infrapatellar fat pad is significantly associated with KOA severity, demonstrating a progressive decrease as the disease advances. These findings suggest that PDFF holds promise as a potential objective biomarker for evaluating KOA severity. However, further validation in larger and more diverse cohorts is required to confirm its clinical applicability. This study is a prospective investigation that adhered to the Declaration of Helsinki and was approved by the Ethics Committee of the First Hospital of Hebei Medical University (Approval No. [2024] Research Review No. 056).</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"288"},"PeriodicalIF":2.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of patellofemoral joint arthroplasty: a systematic review of revision timelines and complication rates.","authors":"Martinique Vella-Baldacchino, Dean Chughtai, Jonathan Kow, Charlotte Carr, Amelie Coyle, Amerlia Farrow-Foster, Jemima Russell, Alexander D Liddle","doi":"10.1186/s13018-025-05592-8","DOIUrl":"10.1186/s13018-025-05592-8","url":null,"abstract":"<p><strong>Introduction: </strong>This systematic review attempts to address survivorship of patellofemoral joint replacements, with conversion to a total knee arthroplasty (TKA) as an endpoint.</p><p><strong>Methods: </strong>Survival estimates from multiple case series and national registries were pooled to calculate survival at 5, 10, 15 and 20 years, respectively. As a secondary outcome, the type and number of complications were recorded. A complication was defined as any any adverse event or unintended outcome that occurs as a result of the joint replacement, either during the immediate postoperative period or over the longer term. As arthroplasty registries do not report this information, this data was only included from publicly available series.</p><p><strong>Results: </strong>1015 eligible articles were identified, with 21 reporting survival and reasons for revision or complications. Data from registries were extracted. Using publicly available results from international joint registries, survival at 5 and 10 years were 90.30% (95% CI 88.32 to 92.27) and 82.23% (95% CI 78.90 to 85.56), respectively. However, long-term survivorship decreased to 73.74% (95% CI 69.12 to 78.37) and 72.68% (95% CI 69.58 to 75.53) at 15 and 20 years.</p><p><strong>Conclusion: </strong>Our pooled data, survival data from case series show similar results to international joint registries up to 10 years with a survival rate of 82.33%. These findings will be of use to patients and arthroplasty surgeons who require further information in order to predict how long patellofemoral joint replacements will last.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"289"},"PeriodicalIF":2.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full-endoscopic posterior lumbar interbody fusion for the treatment of lumbar degenerative diseases: a technical note with 2-year follow-up.","authors":"Lihui Yang, Baodong Wang, Lei Zang, Peng Du, Shuo Yuan, Ning Fan, Qichao Wu","doi":"10.1186/s13018-025-05632-3","DOIUrl":"10.1186/s13018-025-05632-3","url":null,"abstract":"<p><strong>Introduction: </strong>The spinal endoscopic technique has been extensively documented in decompression procedures for treating lumbar degenerative diseases. However, there is limited literature on the spinal endoscopic in lumbar fusion techniques. This study evaluates the outcomes and safety of full-endoscopic posterior lumbar interbody fusion (Endo-PLIF) for the treatment of lumbar degenerative diseases.</p><p><strong>Methods: </strong>A retrospective case series was conducted at Beijing Chaoyang Hospital, Capital Medical University, involving 43 patients who underwent Endo-PLIF between February 2020 and March 2021, with a minimum follow-up period of two years. Clinical outcomes were evaluated using the Visual Analog Scale (VAS), Oswestry Disability Index (ODI), and modified MacNab criteria. VAS and ODI scores were analyzed using one-way analysis of variance (ANOVA) at preoperative, 3-month, 12-month, 24-month, and final follow-up time points. Paired t-tests were employed to compare imaging parameters, including lumbar lordosis, disc height, segmental lordosis, and foraminal area, between the preoperative and final follow-up assessments.</p><p><strong>Results: </strong>This study included 43 consecutive patients with a mean age of 60.7 years and an average symptom duration of 4.6 years. All surgical procedures were successfully completed, with a mean operation duration of 233.8 ± 38.6 min. Mean VAS and ODI scores showed significant improvements postoperatively, decreasing from 7.05 ± 3.05, 7.44 ± 2.95, and 67.52 ± 9.31 points preoperatively to 1.22 ± 0.54, 1.50 ± 0.42, and 20.42 ± 3.57 at the latest follow-up (p < 0.001). Disc height (p = 0.012) and foraminal area (p = 0.013) increased significantly. MacNab evaluation indicated 90.6% of patients had good to excellent outcomes. Three patients experienced symptomatic nerve root irritation.</p><p><strong>Conclusion: </strong>Endo-PLIF is safe and effective in the treatment of patients with lumbar degenerative disease in early follow-up. However, further extensive, long-term, multicenter studies are necessary to validate these findings.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"286"},"PeriodicalIF":2.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic influences of BMP9 and NGF on the osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.","authors":"Junyu Liu, Kun Yang, Gang Li, Yinghui Tan","doi":"10.1186/s13018-025-05669-4","DOIUrl":"10.1186/s13018-025-05669-4","url":null,"abstract":"<p><strong>Background: </strong>Bone morphogenetic protein 9 (BMP9) and nerve growth factor (NGF) are critical factors influencing osteogenic differentiation in mesenchymal stem cells (MSCs). While BMP9 has been recognized for its potent osteogenic capabilities, NGF's role in bone tissue engineering is less understood. This investigation delineated the synergistic link of BMP9 with NGF in driving osteogenic differentiation in C3H10T1/2 MSCs.</p><p><strong>Objective: </strong>To evaluate the combined impact of BMP9 and NGF on osteogenic markers' expression levels and the formation of calcified nodules in C3H10T1/2 cells, providing a basis for the enhanced bone regeneration strategies in tissue engineering.</p><p><strong>Methods: </strong>C3H10T1/2 cells were subjected to treatment regimens incorporating NGF at variable concentrations (10, 50, and 100 ng/ml) and BMP9, either as monotherapies or in combination. Osteogenic differentiation' comprehensive assessment was undertaken by quantifying early-stage markers (Runx2, Col I) and late-stage markers (OPN) via RT-PCR, Western blotting, ALP staining, and Alizarin Red S staining for mineralized matrix deposition.</p><p><strong>Results: </strong>NGF elicited a concentration-dependent augmentation of early osteogenic markers, with the 10 ng/ml dosage demonstrating maximal efficacy. BMP9 independently facilitated robust osteogenic differentiation, whereas the combinatorial treatment with BMP9 and NGF synergistically amplified the expression levels of Runx2, Col I, and OPN. Notably, this combined treatment yielded a remarkable enhancement in the deposition of mineralized extracellular matrix, as evidenced by a notable escalation in the size and density of calcified nodules relative to monotherapies.</p><p><strong>Conclusion: </strong>The findings unveiled the remarkable synergistic link of BMP9 with NGF in potentiating osteogenic differentiation in C3H10T1/2 MSCs. This dual-factor approach presents a compelling paradigm for advancing bone regeneration strategies, providing substantial promise for utilization in bone tissue engineering plus regenerative medicine.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"287"},"PeriodicalIF":2.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lateral unicompartmental knee arthroplasty is an effective procedure for lateral post-meniscectomy knee osteoarthritis: a case-control study at a mean 7-year follow-up.","authors":"Tao Yang, Huaming Xue, Tong Ma, Tao Wen, Long Xue, Yihui Tu","doi":"10.1186/s13018-025-05615-4","DOIUrl":"10.1186/s13018-025-05615-4","url":null,"abstract":"<p><strong>Background: </strong>Meniscectomy is a common knee surgery for meniscal tear, and is associated with progressive osteoarthritis (OA). There are few literatures focus on the use of lateral unicompartmental knee arthroplasty (UKA) for lateral post-meniscectomy knee osteoarthritis (PMKO). Therefore, the purpose of this study is to compare the outcomes of lateral UKA performed for lateral PMKO and primary lateral compartment knee osteoarthritis (LCKO).</p><p><strong>Methods: </strong>A total of 38 consecutive patients (38 knees) who received lateral UKAs for isolated lateral PMKO between September 2013 and September 2019 were retrospectively analyzed. Other thirty-eight patients (38 knees) with primary LCKO were allocated into control group by 1:1 matching according to age, gender, and body mass index. The clinical outcomes were evaluated using the American Knee Society Score, range of motion, Forgotten Joint Score, and EuroQol-5D (EQ-5D) Score. The radiographic assessments included hip-knee-ankle (HKA) angle, mechanical lateral distal femoral angle (mLDFA), mechanical medial proximal tibial angle (mMPTA), and posterior tibial slope angle (PTSA).</p><p><strong>Results: </strong>With a mean 7 years follow-up, there was no significant difference in functional and radiographic outcomes between groups. However, the PMKO group showed severe lateral OA (p = 0.02) preoperatively and less OA progression in the medial compartment postoperatively (p = 0.046). The preoperative mLDFA was significantly more valgus in the LCKO group (p < 0.001). No case of revision occurred in either group.</p><p><strong>Conclusion: </strong>Lateral UKA is a valid procedure for lateral PMKO. The clinical and radiographic results are similar in patients underwent lateral UKA for lateral PMKO and for LCKO. Patients with lateral PMKO exhibited severe lateral OA preoperatively and less OA progression in the medial compartment compared to those with LCKO. It is crucial to prevent ascension of the lateral femoral joint-line and maintain proper valgus alignment during lateral UKA.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"284"},"PeriodicalIF":2.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}