Journal of Orthopaedic Surgery and Research最新文献

{"title":"Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model.","authors":"Junjie Li, HaomingYou, Yucheng Hu, Ruxu Li, Tianxin Ouyang, Qiang Ran, Guilong Zhang, Yong Huang","doi":"10.1186/s13018-025-05504-w","DOIUrl":"10.1186/s13018-025-05504-w","url":null,"abstract":"<p><strong>Background: </strong>The target and mechanism of oral traditional Chinese medicine (TCM) have been important research directions for a long time. The close relationship between osteoporosis and gut microbiota (GM) has been confirmed. However, the relevance of oral TCM and the \"Gut-Bone Axis\" is still poorly understood.</p><p><strong>Methods: </strong>Twenty-one SPF C57BL/6J female mice were divided into sham (Sham), ovariectomized (OVX), and Zuo-Gui-Wan-treated (ZGW, 1.4 g/kg) groups. The osteoporosis mouse model was established through ovariectomy. After eight weeks of Zuo-Gui-Wan treatment via gavage, serum calcium, phosphorus, ALT, AST, CREA, and other biochemical indicators were measured. Subsequently, Micro-CT, HE staining, and analysis of gut microbiota were conducted to further explore the potential mechanism.</p><p><strong>Results: </strong>The anti-osteoporotic effects of ZGW were confirmed through micro-CT, histological, and biochemical tests in an OVX-induced osteoporosis mouse model. ZGW treatment also alters the diversity and composition of the gut microbiota and altered the Firmicutes/Bacteroidetes ratio. Further analysis reveals a correlation between specific bacterial groups and serum indicators. Mfuzz clustering analysis and metagenomeSeq analysis identified important microbiota species that were rescued or modulated by ZGW treatment.</p><p><strong>Conclusion: </strong>These findings suggest that changes in gut microbiota abundance may be linked to ZGW's ability to improve osteoporosis. This study provides new insights into how ZGW treats osteoporosis, though further research is needed to clarify the mechanisms by which specific gut microbiota influence bone health.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"128"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing silicone-coated self-adhesive absorbent polyurethane films with transparent absorbent films for bilateral hip dressing: a prospective randomized controlled trial.","authors":"Chirathit Anusitviwat, Varah Yuenyongviwat","doi":"10.1186/s13018-024-05448-7","DOIUrl":"10.1186/s13018-024-05448-7","url":null,"abstract":"<p><strong>Background: </strong>Silicone-coated self-adhesive absorbent (SSA) and transparent films with absorbent (TFA) dressings are reportedly effective postoperative knee surgery dressings; however, there have been no direct comparative studies on these two innovative dressings over the hip areas. In this study, we aimed to compare user satisfaction and potential complications between TFA and SSA dressings for the hip area.</p><p><strong>Methods: </strong>This prospective randomized controlled trial was conducted at a tertiary hospital. The hip side to receive the polyurethane film with SSA dressing (Mepilex^® Border Post-Op) was randomly allocated. The other side of the hip was covered with TFA (OPSITE Post-Op). Participants were scheduled for follow-ups 7 and 14 days after the initial application. Between-group outcomes were compared using a two-sample t-test or Wilcoxon signed-rank test for continuous variables and McNemar's chi-square test for categorical variables.</p><p><strong>Results: </strong>Thirty-two participants (30 - 60 years) without a history of hip surgery were included in the study. The participants were predominantly female, with a mean age of 42.8 years. Pain, difficulties in daily activities, and satisfaction scores were similar between the groups. However, moisture accumulation was significantly higher with the TFA dressing (37.9% vs. 13.8%, p < 0.01), with more dressing failures (34.5% vs. 20.7%, p = 0.016) and complications (37.9% vs. 17.2%, p = 0.012) at the 14-day follow-up than with the SSA dressing.</p><p><strong>Conclusions: </strong>SSA dressings are preferable for hip wound care because of better moisture management, fewer dressing changes required, and fewer complications if applied for > 7 days. Both dressings offered high user satisfaction, minimal pain, and minor difficulties in daily activities.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"129"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A modified non-contrast MRI in the preoperative examination of periacetabular osteotomy at 3.0T.","authors":"Chuanhui Xu, Xin Pan, Baochen Song, Guangwei Zhang, Daguang Zhang","doi":"10.1186/s13018-025-05521-9","DOIUrl":"10.1186/s13018-025-05521-9","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate whether the modified hip non-contrast magnetic resonance imaging (MRI) can replace the direct MRI hip arthrography (d-MRA) at 3.0T in developmental dysplasia of the hip (DDH) patients undergoing periacetabular osteotomy (PAO) preoperative examination, and also to assess the difference between these two methods.</p><p><strong>Materials and methods: </strong>This retrospective study involved 35 patients (38 hips) with DDH who underwent periacetabular osteotomy, age 9-41 years old (mean age 25), 4 males (4 hips) and 34 females (34 hips). Each patient underwent both d-MRA and modified 3.0T non-contrast MRI examination of the hip joint. All patients underwent hip-preserving PAO surgery subsequently. Statistical analyses were performed to compare the joint injury examined by the two imaging methods.</p><p><strong>Results: </strong>The modified non-contrast MRI detected the labral injury with a sensitivity of 94%, a specificity of 75%, an accuracy of 92%, and a positive predictive value of 97%. Among the 38 hips, 10 hips had labral varus (i.e., inward deviation or angulation of the acetabular labrum) with an incidence rate of 26% (10/38), 9 hips had acetabular cysts (i.e., the fluid-filled sacs that develop in or around the acetabulum, the socket of the hip joint) with an incidence rate of 23% (9/38). One patient had both the labral cyst and acetabular cartilage injury, and two patients had labral cyst only (overall incidence rate of labral cyst 7% (3/38)). The results were comparable to those of d-MRA in the examination of cartilage injury, labral varus, acetabular cyst, and labral cyst.</p><p><strong>Conclusion: </strong>For preoperative hip-preserving surgery in DDH patients, the modified non-contrast MRI performed similarly to the d-MRA at 3.0T in diagnostic capabilities. The labral injury mostly occurs in the anterior upper quadrant.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"121"},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effect and experimental research progress of pleotropic statins in intervertebral disc degeneration.","authors":"Xianxu Zhang, Shicheng Li, Xin Liu, Zhong Ma, Qiang Zhang, Yuji Zhang, Jiangdong An, Zhiqiang Luo","doi":"10.1186/s13018-025-05487-8","DOIUrl":"10.1186/s13018-025-05487-8","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IVDD) is one of the primary causes of low back pain, significantly affecting the quality of life in the elderly population and imposing a substantial economic burden. Currently, clinical treatments for IVDD mainly focus on symptom management, with no available therapies capable of reversing or curing the disease. In recent years, statins, a class of drugs traditionally used in the treatment of cardiovascular diseases, have been shown to exert beneficial effects on IVDD through multiple mechanisms. With the advancement of research into the pleiotropic effects of statins, an increasing body of evidence suggests their potential as effective therapeutic agents for IVDD. This review summarizes the pleiotropic effects of statins and explores their potential mechanisms and actions in IVDD, with particular emphasis on changes in the expression of relevant molecular markers. Furthermore, recent advancements in the application of statins for IVDD treatment are discussed, along with the potential of combining statins with other drugs as part of multi-target therapeutic strategies. This review aims to provide a scientific reference for further investigations into the use of statins in the treatment of IVDD.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"122"},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of conservative treatment on greater trochanteric pain syndrome: a systematic review and network meta-analysis of randomized controlled trials.","authors":"Si-Qi Wang, Ning-Yi Guo, Wei Liu, Hong-Jie Huang, Bing-Bing Xu, Jian-Quan Wang","doi":"10.1186/s13018-025-05477-w","DOIUrl":"10.1186/s13018-025-05477-w","url":null,"abstract":"<p><strong>Objective: </strong>Numerous studies have investigated conservative treatments for GTPS. However, there remains a lack of comparative analyses among treatment modalities, making it challenging to formulate the optimal conservative treatment strategy. This study aimed to compare conservative treatments for greater trochanteric pain syndrome (GTPS) in terms of their effectiveness in relieving pain and improving functional outcomes.</p><p><strong>Methods: </strong>In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, the PubMed, Embase, Cochrane Library and ClinicalTrials.gov along with other databases were searched to identify relevant studies. The quality of the selected studies was evaluated using risk of bias assessments, and the data were extracted. Network meta-analysis was performed using random effects models to evaluate the effects of treatment modalities on pain reduction and functional improvement. The evidence of the included studies was evaluated using the Grading of Recommendations, Assessment, Development, and Evaluations framework.</p><p><strong>Results: </strong>Nineteen RCTs involving 1701 participants were included. The risk of bias was assessed using Cochrane ROB2. The results of network meta-analysis revealed that exercise therapy yielded the most significant improvement on reducing pain scores measured using the NRS and enhancing functional outcomes measured using the VISA-G. Additionally, injection therapy showed significant advantages in improving functional outcomes measured by using the Harris Hip Scale.</p><p><strong>Discussion: </strong>This study confirmed the significant efficacy of exercise programs on pain reduction and functional improvement in GTPS patients. Additionally, we identified the positive effects of injection therapy and physical modalities on managing pain and enhancing functional outcomes among GTPS patients. Owing to the limited number of included studies, comparing the effects of different conservative treatments remains challenging. Future studies should expand the quantity of RCTs to identify the optimal conservative treatment strategy for GTPS.</p><p><strong>Conclusion: </strong>This study reaffirmed the superiority of exercise therapy in the treatment of GTPS and revealed the effectiveness of injection and physical modalities. As the number of studies increase, we anticipate more reliable conclusions to further optimize conservative treatment strategies for GTPS. Ultimately, our study can help clinicians determine the optimal conservative treatment for GTPS and may spur future research on conservative treatments for tendinopathy.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"126"},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone marrow lesion and medial meniscus extrusion width changes following arthroscopic partial meniscectomy for medial meniscal flap tears.","authors":"Hitoshi Sekiya, Kenzo Takatoku, Hitoshi Okami, Yuji Kanaya, Kenta Yanagisawa","doi":"10.1186/s13018-025-05535-3","DOIUrl":"10.1186/s13018-025-05535-3","url":null,"abstract":"<p><strong>Background: </strong>The current literature evaluating meniscectomy outcomes often mixes various tear patterns, failing to elucidate the impact of specific tear types on procedure results. Flap tears, which are prone to causing mechanical symptoms, require targeted research. This study aims to examine Lysholm scores following a meniscectomy for medial meniscus flap tears, to determine if extensive resections lead to poorer one-year outcomes, and to assess their impact on postoperative bone marrow lesion (BML) risk and medial meniscus extrusion (MME) width.</p><p><strong>Methods: </strong>Patients undergoing arthroscopic meniscectomy for medial meniscal flap tears were classified into three groups: minimum resection, single leaf resection, and subtotal resection. Lysholm scores and medial joint space (MJS) width on Rosenberg view radiographs were measured preoperatively and one year postoperatively. BMLs and MMEs were assessed via MRI preoperatively and at 1, 3, 6, and 12 months postoperatively.</p><p><strong>Results: </strong>Fifty patients (mean age 60 ± 13 years) underwent meniscectomy: minimum resection in 21 (42%), single leaf in 23 (46%), and subtotal in 6 (12%). The Lysholm scores improved significantly, from 65.5 ± 17.6 to 93.4 ± 16.5 (n = 50) at 12 months in patients overall (p < 0.001), while MJS width decreased from 3.9 ± 0.7 mm to 3.5 ± 0.8 mm (p < 0.001). There were no significant differences in Lysholm scores or MJS widths among the three resection patterns. The occurrence rate of BMLs in the medial femoral condyle and tibial plateau increased post-surgery, peaking at 1 month, but then improved to near-baseline by 12 months. The occurrence rate of BMLs was higher in the single leaf and subtotal meniscectomy groups post-surgery, but declined across all groups by 12 months. Among the three groups with different resection patterns, MME width at 12 months was 2.7 ± 1.8 mm in the subtotal resection group, which was significantly larger than in the other groups.</p><p><strong>Conclusions: </strong>Arthroscopic partial meniscectomy for flap tears yielded favorable 12-month outcomes. Larger resections were associated with greater BML incidence and greater MME width.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"123"},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of acupuncture for primary osteoporosis: a systematic review and meta-analysis of randomized controlled trials.","authors":"Tianyi Ma, Tiantian Zhang, Le Zhang, Haoming Zhao, Ke Liu, Jianjun Kuang, Liang Ou","doi":"10.1186/s13018-025-05513-9","DOIUrl":"10.1186/s13018-025-05513-9","url":null,"abstract":"<p><strong>Background: </strong>Primary osteoporosis (POP) is a common metabolic bone disorder that has a devastating effect on their quality of life in patients. Acupuncture, a traditional Chinese therapy, has been used to treat osteoporosis for over 2000 years. This study aimed to determine the efficacy of acupuncture in treating POP compared to conventional medicine or placebo.</p><p><strong>Methods: </strong>We searched for potentially relevant studies in PubMed, Web of Science, Embase, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, China Biology Medicine disc, Wanfang database and ClinicalTrials.gov up to December 20, 2024. Randomized controlled trials investigating treatment of POP for which acupuncture was administered as a stand-alone treatment or combined with conventional medicine compared to conventional medicine or placebo, were included. The outcomes included bone mineral density (BMD), visual analogue scale (VAS) scores, clinical effectiveness rate, estradiol (E₂), Oswestry Disability Index (ODI), and levels of serum alkaline phosphatase (ALP). Data were synthesized using a random-effects meta-analysis model, and the observed heterogeneity was investigated using subgroup analyses. Study quality was appraised using the Cochrane RoB 2 tools, and the quality of the aggregated evidence was evaluated using the GRADE guidelines. Publication bias was assessed by funnel plots and validated by Egger's test.</p><p><strong>Results: </strong>Forty eligible articles with 2654 participants were identified. Compared to the control group, acupuncture effectively increased the BMD (MD 0.04 [0.03-0.06], P < 0.001, I² = 92%), clinical efficacy (RR 1.24 [1.14-1.34], P < 0.001, I² = 81%), and levels of E₂ (SMD 0.30 [0.09-0.52], P = 0.006, I² = 0%), and reduced the VAS scores (SMD - 1.79 [- 2.29 to - 1.29], P < 0.001, I² = 95%). Data on ODI and ALP were insufficient for meta-analysis.</p><p><strong>Conclusion: </strong>The current evidence suggests that the efficacy of acupuncture in improving the symptoms of POP are encouraging for its use in clinical practice as a physical intervention for patients with POP. However, since the included patients were all from China, there was a risk of sample bias, high-quality multicenter studies in different countries or regions should be conducted in the future.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"127"},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Justification of Body Mass Index cutoffs for hip and knee joint arthroplasty among California orthopedic surgeons.","authors":"Sophie V Orr, Gavin C Pereira, Blaine A Christiansen","doi":"10.1186/s13018-025-05551-3","DOIUrl":"10.1186/s13018-025-05551-3","url":null,"abstract":"<p><strong>Background: </strong>Many orthopedic surgeons choose not to perform joint arthroplasty on patients with a Body Mass Index (BMI) of 35 or above, citing poorer outcomes and increased procedure risk. Identifying and addressing factors surgeons use to determine procedure BMI cutoffs are necessary to increase access to orthopaedic care for this growing patient population. This will help reduce healthcare disparities while also identifying clinical facilities, equipment, training, and procedures that require improvements to accommodate larger individuals.</p><p><strong>Methods: </strong>Orthopaedic surgeons were surveyed to identify surgeon-specific BMI cutoffs for hip and knee arthroplasty. The survey was circulated within the California Orthopaedic Association (COA) report during March 2023. Questions aimed to identify BMI cutoffs and justifications such as infection risk, co-morbidities, inadequate equipment, and the American Academy of Orthopaedic Surgeons (AAOS) guidelines. Data on decision making about BMI cutoffs and exceptions were also collected.</p><p><strong>Results: </strong>75% of respondents use BMI cutoffs for hip and knee arthroplasty. 91% of respondents indicated they are either wholly or partially responsible for setting procedure BMI cutoffs. Mean hip and knee arthroplasty BMI cutoffs were 40.5 and 41, respectively. Four categories for BMI cutoff justifications were identified: (1) risk of complications; (2) surgery logistics; (3) concerns about facilities or resources; and (4) surgeon perception.</p><p><strong>Conclusions: </strong>BMI-based justifications for denial of care define key addressable areas of improvement that can increase access to care for life-changing orthopaedic surgeries such as THA and TKA. Insight from the queried surgeons will help drive future research areas to address this need.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"125"},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail.","authors":"Rongbing Shu, Zhuanyi Yu, Jianmin Wu, Qiuxin Cheng, Zhihao Peng, Huaqiang Zhou, Min Zhao","doi":"10.1186/s13018-024-05412-5","DOIUrl":"10.1186/s13018-024-05412-5","url":null,"abstract":"<p><strong>Objective: </strong>Osteosarcoma (OS) is a highly invasive bone tumor that frequently metastasizes to the lungs. This study aims to investigate the role of the Id-1 gene in OS invasion and metastasis, and its relationship with the Snail gene.</p><p><strong>Methods: </strong>This study included tissue samples from 12 non-metastatic osteosarcomas and 9 metastatic osteosarcoma patients to examine the expression of Id-1 and Snail using RT-qPCR and analyze their correlation. In cell-based experiments, four osteosarcoma cell lines (Saos-2, U2OS, MG-63, and 143B) and the human osteoblast cell line hFOB 1.19 were cultured. The expression of Id-1 and Snail was evaluated by RT-qPCR and Western blotting.Cells were randomly divided into the Control group, sh-NC group, and sh-Id-1 group using lentiviral infection. Transwell invasion and scratch assays were used to assess cell migration and invasion. WB was employed to detect the expression of Id-1, Snail, and epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin, vimentin, and N-cadherin) in the OS cells of each group. In animal experiments, Tumor formation in each group was evaluated by injecting cells subcutaneously into mice. An osteosarcoma lung metastasis model was established by injecting infected cells into the tibia of mice. Tumor growth and lung metastasis were observed using HE staining. The expression of Id-1, Snail, and EMT-related proteins in osteosarcoma and lung tissues from each group of mice was assessed using Western blot and immunohistochemistry.</p><p><strong>Results: </strong>The expression of Id-1 and Snail was significantly higher in osteosarcoma tissues than in normal bone tissues, and the expression of Id-1 was positively correlated with that of Snail. In cell experiments, downregulation of Id-1 reduced Snail expression and significantly inhibited EMT, as well as the migration and invasion of OS cells (P < 0.05). In animal experiments, compared to the Control group, the sh-Id-1 group mice was no significant change in body weight, but the tumor volume was significantly reduced, and fewer lung metastatic nodules (P < 0.05). HE staining indicated decreased nuclear atypia, reduced invasion and destruction, fewer new blood vessels, and less calcification in the sh-Id-1 group tumors. Immunohistochemistry and WB results showed upregulation of E-cadherin and downregulation of vimentin, N-cadherin, Id-1, and Snail in the sh-Id-1 group (P < 0.05).</p><p><strong>Conclusion: </strong>Downregulation of Id-1 inhibits the EMT process by reducing Snail expression, effectively suppressing the growth, invasion, and lung metastasis of OS.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"124"},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors, characteristics and influences of the changes of muscle activation patterns for patients with knee osteoarthritis: a review.","authors":"Shizhong Liu, Zuyu Du, Le Song, Haoyue Liu, Clarence Augustine T H Tee, Huanyu Liu, Yuan Liu","doi":"10.1186/s13018-025-05484-x","DOIUrl":"10.1186/s13018-025-05484-x","url":null,"abstract":"<p><strong>Background: </strong>Knee Osteoarthritis (KOA) is a prevalent condition worldwide, significantly diminishing quality of life and productivity. Except for the alignment change, muscle activation patterns (MAP) have garnered increasing attention as another crucial factor contributing to KOA.</p><p><strong>Objective: </strong>This study explores the factors, characteristics, and effects of MAP changes caused by KOA, providing a neuromuscular-based causal analysis for the rehabilitation treatment of KOA.</p><p><strong>Methods: </strong>Keywords including the association of MAP with KOA will be included. \"Knee, Osteoarthritis, Electromyography(EMG), Muscle Activity patterns, activation amplitudes, activation time, Muscle Synergy, Co-contraction/activation\" were used to search the databases of Science Direct, PubMed, Scopus, and Wiley. The criteria include studies from the past fifteen years that document changes in muscle contraction characteristics and causality analysis in patients with KOA. we compared MAP changes between individuals with and without KOA, such as the activation amplitudes, activation time, muscle synergy and co-contraction index(CCI). Additionally, we explored the potential relationship between muscle weakness, pain, and lower limb mechanical changes with the variations of MAP.</p><p><strong>Results: </strong>A total of 832 articles were reviewed, and 44 articles that met the inclusion criteria were selected for analysis. The changes in biomechanical structure, pain, and muscle atrophy may contribute to the formation and progression of the changes in MAP in KOA patients. In moderate KOA patients, the vastus lateralis (VL) and biceps femoris (BF) exhibits larger activation amplitudes, with earlier and longer activation times. The vastus medialis (VM) shows a delayed activation time relative to VL. Gastrocnemius activation time is prolonged during mid-gait, while the soleus exhibits lower activation amplitudes during the late stance phase. There are fewer, merged synergies with prolonged activation coefficients, and a higher percentage of unclassifiable synergies. Additionally, the CCI is positively correlated with task difficulty and symptoms. It is higher in the medial and lateral than hamstrings and quadriceps, and CCI specifically respond to joint stabilisation and load.</p><p><strong>Conclusion: </strong>In patients with moderate KOA, changes in MAP are mainly related to symptoms and the difficulty of tasks. MAP changes primarily result in variations in amplitude, contraction duration, muscle synergy, and CCI. The MAP changes can subsequently affect the intermuscular structure, pain, joint loading, and stiffness.</p><p><strong>Clinical implications: </strong>These contribute to the progression of KOA and create a vicious cycle that accelerates disease advancement. Clinical rehabilitation treatments can target the MAP changes to break the cycle and help mitigate disease progression.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"112"},"PeriodicalIF":2.8,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}