Journal of Orthopaedic Surgery and Research最新文献

{"title":"One-stage posterior debridement approach combined with autogenous bone grafting and internal fixation for the treatment of adult thoracic or lumbar suppurative spondylitis via the multifidus and longissimus interspaces.","authors":"Yanjiang Guo, Yunbo Yang, Zan Chen, Daxiong Feng, Fei Lei","doi":"10.1186/s13018-025-05735-x","DOIUrl":"https://doi.org/10.1186/s13018-025-05735-x","url":null,"abstract":"<p><strong>Background: </strong>Surgical treatment of suppurative spondylitis requires focus debridement, spinal stability, and deformity correction. Different approaches face challenges like complex anatomical structures and greater trauma. The aim is to evaluate the clinical efficacy of One-stage debridement approach combined with autogenous bone grafting and internal fixation for the treatment of adult thoracic or lumbar suppurative spondylitis via the multifidus and longissimus interspaces.</p><p><strong>Methods: </strong>From July 2018 to July 2021, 91 patients (65 male, 26 female) with single - level thoracic or lumbar suppurative spondylitis underwent the one-stage posterior procedure. Operative details and hospital stay were recorded. ESR, CRP, and PCT levels were analyzed at specific times. Pain was rated by VAS, Using ODI score to evaluate the improvement of lumbar activity, and neurological function was assessed by ASIA scale. Cobb angles were measured for deformity evaluation. Bony fusion was evaluated by radiography and computed tomography.</p><p><strong>Results: </strong>The mean operative duration was 195.6 ± 15.4 min, blood loss was 575.9 ± 90.1 ml, and hospital stay was 19.9 ± 2.2 days. ESR, CRP, and PCT levels significantly decreased before discharge (ESR: 80.2 ± 14.6 mm/h vs. 30.2 ± 8.9 mm/h, CRP: 58.5 ± 13.6 mg/L vs. 15.1 ± 7.4 mg/L, PCT: 0.8 ± 0.2 ng/ml vs. 0.1 ± 0.1 ng/ml, P < 0.05). All patients had pain relief. VAS scores improved (preoperative 7.7 ± 1.1, before discharge 2.8 ± 0.6, final follow-up 1.3 ± 0.6). The lumbar activity of all patients was significantly improved compared with preoperative(preoperative 41.36 ± 3.20, final follow-up 6.18 ± 1.33). Neurologically impaired patients improved in ASIA grade. The mean preoperative Cobb angle was 19.6 ± 1.6°, reduced to 6.2 ± 1.5° before discharge and 9.4 ± 1.0° at final follow-up. The mean angle correction was 13.4 ± 0.6°, correction rate 68.7% ± 5.4% (P < 0.05), with a final loss angle of 3.2 ± 0.7° and loss rate 24.1% ± 4.8%. All grafts achieved complete fusion.</p><p><strong>Conclusions: </strong>This one-stage posterior approach is effective and feasible for adult single - level thoracic or lumbar suppurative spondylitis, protecting paravertebral muscles and posterior ligament complexes. However, further studies with larger samples and longer follow-up are needed.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"330"},"PeriodicalIF":2.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and influencing factors of kinesiophobia after total knee arthroplasty: a systematic review and meta-analysis.","authors":"Xingbin Du, Yuanwei Shao, Jian Xue, Jianda Kong","doi":"10.1186/s13018-025-05752-w","DOIUrl":"https://doi.org/10.1186/s13018-025-05752-w","url":null,"abstract":"<p><strong>Background: </strong>Knee osteoarthritis is a common degenerative disease in the elderly, and total knee arthroplasty is an effective treatment for end-stage knee joint diseases. However, kinesiophobia after surgery can impede patients' rehabilitation and affect the recovery of knee joint function. There are differences in the research on its related influencing factors.</p><p><strong>Objectives: </strong>This meta-analysis examined the prevalence and risk factors of kinesiophobia after TKA.</p><p><strong>Methods: </strong>Pubmed, The Cochrane Library, China National Knowledge Infrastructure (CNKI), Embase, Web of Science on the prevalence and risk factors of kinesiophobia after TKA was searched in science, MEDLINE and other databases. Duplicate literature, low quality literature, literature with inconsistent observation indicators, and literature without full text were excluded. Two independent researchers used Newcastle-Ottawa Scale (NOS) to evaluate the quality of the included literature. After data extraction, Meta-analysis was performed using Stata17.0.</p><p><strong>Results: </strong>A total of 11 articles involving 4039 cases were included in this meta-analysis to assess the prevalence of kinesiophobia after TKA. The overall prevalence was found to be 35% (95% CI: 27-44%). Subgroup analyses revealed varying prevalence rates based on age, education, income, and residence, with the highest prevalence observed in individuals under 65 years and those with lower levels of education and income. Key factors influencing the prevalence of kinesiophobia included pain (OR = 2.313, 95% CI: 1.556-3.07), low social support (OR = 1.681, 95% CI: 1.000-2.361), and negative coping strategies (OR = 1.344, 95% CI: 1.165-1.523).</p><p><strong>Conclusion: </strong>The prevalence of kinesiophobia after TKA is high. There are differences in the prevalence of kinesiophobia among people with different places of residence, different education levels, and different monthly incomes. At the same time, it is affected by many factors such as postoperative pain, low social support, low self-efficacy, negative coping, old age, and low education level.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"332"},"PeriodicalIF":2.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive validation of a custom three-point bending system for standardized diabetic fracture models in rats.","authors":"Qidong Guo, Weijie Wang, Zheng Guo","doi":"10.1186/s13018-025-05754-8","DOIUrl":"https://doi.org/10.1186/s13018-025-05754-8","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus (DM) significantly impairs fracture healing, necessitating reliable animal models to study diabetic fracture repair mechanisms and therapeutic interventions. This study aimed to develop and comprehensively validate a standardized three-point bending system for inducing precise, reproducible mid-shaft transverse femoral fractures in rats, addressing existing methodological gaps of insufficient reproducibility and detailed calibration in previous models.</p><p><strong>Methods: </strong>A custom-designed three-point bending fracture induction system was developed using AutoCAD software based on the original principle introduced by Einhorn et al. (1984). After manufacturing and calibration, the system was validated first using 22 cadaveric rat femurs and subsequently applied to live rats (n = 44), including diabetic (streptozotocin-induced, n = 22) and non-diabetic animals (n = 22). Fracture induction reproducibility was assessed through radiographic, histologic and mechanic analysis. Additionally, statistical analysis was conducted using GraphPad Prism 9 software. Coefficients of variation (CV) for fracture-healing parameters (callus diameter, calcification ratio, maximum bending force at re-fracture) were calculated and compared statistically with similar parameters from previously published rat femoral fracture studies.</p><p><strong>Results: </strong>Cadaveric validation confirmed that the optimal blade travel distance, set as half the femoral diameter, consistently produced standardized transverse fractures without comminution. A significant correlation between body weight and femoral diameter (Femoral diameter = 1.0276 ln [Body weight] - 1.349) allowed accurate preoperative determination of optimal blade travel distance. Live animal testing demonstrated consistent fracture patterns, stable intramedullary pin fixation, and no complications during surgical procedures. Statistical analysis revealed significantly lower coefficients of variation for healing parameters in this study compared to previously published models (p < 0.05). Histological analysis indicated the fracture type was transverse. Callus was found around fracture site.</p><p><strong>Conclusion: </strong>Our validated three-point bending system significantly enhances reproducibility, consistency, and methodological rigor in animal fracture research. This standardized model provides an ideal foundation for future preclinical studies investigating diabetic fracture healing mechanisms and potential therapeutic interventions.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"328"},"PeriodicalIF":2.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of microRNA-328-3p and bone metabolism biomarkers in gout patients with different musculoskeletal ultrasonography imaging.","authors":"Qingqing Song, Lifang Xue, Jie Ren, Xiaoyu Liu, Guilei Li, Congcong Liu, Xin Meng","doi":"10.1186/s13018-025-05691-6","DOIUrl":"https://doi.org/10.1186/s13018-025-05691-6","url":null,"abstract":"<p><strong>Aims: </strong>MicroRNA (miRNA) participates in the pathophysiological processes of multiple metabolic diseases, including gout. In gout patients, there is concomitant derangement of bone metabolism. The study aimed to explore the correlation of different ultrasonic manifestations and miR-328-3p levels with bone metabolic markers in gout patients.</p><p><strong>Methods: </strong>A total of 320 gout patients were grouped according to musculoskeletal ultrasound (MSUS) imaging. Serum osteocalcin (BGP), C-terminal telopeptide of type I collagen (CTX-I) and osteopotin (OPN) levels were detected to evaluate bone metabolism. Serum miR-328-3p levels were detected via qRT-PCR. Pearson's correlation analysis was performed to explore the relationships between the variables.</p><p><strong>Results: </strong>Patients with tophi accompanied by bone erosion demonstrated significantly elevated levels of BGP and OPN compared to those with tophi or aggregate of MSU crystals in the absence of bone erosions. Cases with long course of disease exhibited more severe bone destruction. Cases without specific clinical manifestations presented the highest levels of serum miR-328-3p, whereas those with bone erosions demonstrated the lowest values. Significantly negative correlations were also detected for serum miR-328-3p levels with BGP and OPN values in all gout patients.</p><p><strong>Conclusion: </strong>Serum miR-328-3p levels were associated with diverse MSUS manifestations in gout patients. MSUS imaging and miR-328-3p levels are capable of reflecting the joint impairment in gout patients.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"329"},"PeriodicalIF":2.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of intraoperative neurophysiological monitoring in unilateral biportal endoscopic lumbar spine surgery.","authors":"Tian-Yu Bai, Hai Meng, Ji-Sheng Lin, Zi-Han Fan, Qi Fei","doi":"10.1186/s13018-025-05738-8","DOIUrl":"https://doi.org/10.1186/s13018-025-05738-8","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the value of intraoperative neurophysiological monitoring (IONM) in unilateral biportal endoscopic (UBE) lumbar spine surgery.</p><p><strong>Methods: </strong>A retrospective analysis was performed on 127 patients who underwent UBE lumbar spine surgery at Xicheng Branch of Beijing Friendship Hospital from January 2024 to September 2024. Patients were divided into two groups: the observation group (IONM, 64 cases) and the control group (no IONM, 63 cases). Changes of monitoring indicators included somatosensory evoked potentials (SEP), motor evoked potentials (MEP), and free electromyography (freeEMG) were recorded. Age, sex, body mass index, surgery length, surgical levels, surgery time, intraoperative fluid volume, post anesthesia care unit time, time to first ambulation, length of hospital stay, leg visual analog scale (VAS), preoperative and postoperative fall scores, activities of daily living, and postoperative complication of two groups were collected and compared.</p><p><strong>Results: </strong>In the observation group, 40 cases (62.5%) showed freeEMG stimulation. 10 cases (15.6%) had a significant decrease in MEP amplitudes, with 9 cases showing a decline in MEP amplitudes immediately following freeEMG stimulation. No significant changes in SEP. The postoperative 24-hour leg VAS in the observation group was 1.8 ± 0.4, which was significantly lower than the 2.1 ± 0.2 in the control group (p < 0.001). No significant differences were found between the two groups in terms of surgical time and other data (p > 0.05).</p><p><strong>Conclusion: </strong>IONM provides timely information of neurological function in UBE lumbar spine surgery, reduces the invasiveness of intraoperative procedures, and reduce early postoperative leg pain.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"334"},"PeriodicalIF":2.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of three surgical methods for displaced posterior cruciate ligament tibial insertion avulsion fractures: a retrospective study.","authors":"Gengao Wen, Congliang Chen, Song Wang, Zhuping Jiang, Shuo Feng, Wei Zheng","doi":"10.1186/s13018-025-05703-5","DOIUrl":"https://doi.org/10.1186/s13018-025-05703-5","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to evaluate the clinical outcomes of three surgical methods, three types of arthroscopic suture fixation, suspension fixation with EndoButton, and open screw fixation, in the treatment of displaced posterior cruciate ligament tibial insertion avulsion fractures.</p><p><strong>Methods: </strong>A retrospective analysis of the clinical data of 104 patients with posterior cruciate ligament avulsion fractures between 2010 and 2023 was performed. The patients were divided into three groups according to the surgical approach used: suture fixation in Group A (24 patients), suspension fixation with EndoButton in Group B (37 patients), and hollow screw fixation in Group C (43 patients). The Lysholm score, International Knee Documentation Committee (IKDC) score, Tegner activity score, and postoperative imaging findings of all patients were collected. The final follow-up data were used to compare the differences among the three groups.</p><p><strong>Results: </strong>There was no statistically significant difference in the general information of the three groups of patients. Among the 104 patients, the mean postoperative Lysholm score was 92.9 (CI, 91.0-94.8) in Group A, 93.4 (CI, 92.3-94.6) in Group B, and 93.5 (CI, 92.6-94.4) in Group C. Postoperative IKDC subjective assessment of knee function was considered normal or near normal in more than 95% of the patients in all three groups. More than 93% of the patients in all three groups did not have severe abnormalities in knee range of motion. The mean postoperative Tegner activity score was 6.7 (CI, 6.3-7.1) in Group A, 7.0 (CI, 6.7-7.3) in Group B, and 6.9 (CI, 6.6-7.1) in Group C. Imaging at 3 months after surgery revealed bone healing, and no serious postoperative complications were found in any of the three groups.</p><p><strong>Conclusion: </strong>All three treatments yielded good clinical results and imaging healing. In particular, arthroscopic suspension fixation with EndoButton is recommended as the preferred treatment because of its low invasiveness, good mechanical properties and low complication rate.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"333"},"PeriodicalIF":2.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does halofuginone influence regeneration after peripheral nerve injury?","authors":"Muzaffer Çaydere, Ömer Şahin","doi":"10.1186/s13018-025-05758-4","DOIUrl":"https://doi.org/10.1186/s13018-025-05758-4","url":null,"abstract":"<p><strong>Background: </strong>Halofuginone is an antiprotozoal drug with antifibrotic and anti-inflammatory properties. The aim of our study was to determine the effects of halofuginone on nerve recovery in sciatic nerve injury and compare it with steroid treatment.</p><p><strong>Methods: </strong>The left sciatic nerves of Sham subjects were exposed without intervention. The nerves of trauma animals were transected and sutured. In the methylprednisolone group and in the trauma group, after nerve transection and repair, 1 mg/kg methylprednisolone per day was administered intraperitoneally for seven days; in the halofuginone group and in the trauma group, after nerve transection and repair, 0.2 mg/kg halofuginone per day was administered orally by gavage for seven days. The rats were functionally evaluated at 4 and 8 weeks through walking path analysis. Pathological-morphometric, immunostaining-quantitative, and muscle weight measurements were performed at 8 weeks.</p><p><strong>Results: </strong>Compared with the trauma group, the methylprednisolone and the halofuginone groups had better functional outcomes (p < 0.001). Statistically significant difference was found in comparisons of the pathological and immunostaining results of the methylprednisolone and halofuginone groups (Respectively, nerve diameter (p = 0.007) and edema (P = 0.009)).</p><p><strong>Conclusion: </strong>Halofuginone positively contributed to recovery after sciatic nerve injury.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"331"},"PeriodicalIF":2.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virtual and augmented reality for anxiety reduction in orthopedic patients and providers: a systematic review.","authors":"Negarsadat Namazi, Yashar Khani, Amirhossein Salmannezhad, Mohammad Behdadfard, Ehsan Safaee, Mohammad Nouroozi, Amir Mehrvar","doi":"10.1186/s13018-025-05690-7","DOIUrl":"10.1186/s13018-025-05690-7","url":null,"abstract":"<p><strong>Background: </strong>Anxiety impacts patients and healthcare providers during orthopedic procedures, yet virtual reality (VR) and augmented reality (AR) effectiveness remains inconsistently reported, lacking systematic synthesis in this setting. This review addresses this gap.</p><p><strong>Methods: </strong>Per PRISMA guidelines (PROSPERO: CRD42024553394), we searched PubMed, Scopus, Web of Science, and Embase in March 2024 for studies on VR/AR/mixed reality (MR) interventions for anxiety in orthopedic procedures. Data were narratively synthesized; bias assessed via RoB-2 and ROBINS-I.</p><p><strong>Results: </strong>Twenty-four studies (16 RCTs, 8 cohort, n = 1714) showed VR (22 studies) and AR (2 studies) significantly reduced anxiety across procedure phases, notably in pediatrics. Healthcare providers (HCPs) reported lower anxiety and higher confidence with VR. Satisfaction rose, anesthetic use dropped, though inconsistent tools and methods limited comparisons.</p><p><strong>Conclusion: </strong>VR/AR reduce pediatric anxiety in orthopedics, with less conclusive adult/HCP benefits. Clinicians could adopt preoperative VR. Research needs standardized tools and adult-focused RCTs.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"327"},"PeriodicalIF":2.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Evaluation and testing of polymethylmetacrylic (PMMA) bone cements with admixed Amphotericin B.","authors":"Florian A Frank, Barbara Krampitz, Julia Steiner, Rainer Strathausen, Mario Morgenstern, Martin Clauss, Klaus-Dieter Kühn","doi":"10.1186/s13018-025-05643-0","DOIUrl":"https://doi.org/10.1186/s13018-025-05643-0","url":null,"abstract":"","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"325"},"PeriodicalIF":2.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-103-3p regulates chondrocyte autophagy, apoptosis, and ECM degradation through the PI3K/Akt/mTOR pathway by targeting CPEB3.","authors":"Jun Li, Farui Sun, Yuanjin Zhang, Xian Pan, Bo Li, Guofu Zhang, Qian Zhou","doi":"10.1186/s13018-025-05719-x","DOIUrl":"https://doi.org/10.1186/s13018-025-05719-x","url":null,"abstract":"<p><strong>Background: </strong>Chondrocyte apoptosis is associated with the severity of cartilage destruction and matrix degeneration in the progression of osteoarthritis. Increasing evidence indicates that autophagy has a significant cytoprotective effect against chondrocyte apoptosis. Here, we investigated the role of microRNA-103-3p (miR-103-3p) in regulating chondrocyte function and elucidated the underlying mechanism.</p><p><strong>Methods: </strong>MiR-103-3p expression in interleukin-1β (IL-1β)-stimulated chondrocytes was evaluated using RT-qPCR. The targets of miR-103-3p predicted by online databases were verified using biotin-based pulldown assay and luciferase reporter assay. IL-1β stimulated-chondrocytes were transfected with miR-103-3p inhibitor along with siRNA targeting cytoplasmic polyadenylation element-binding protein3 (siCPEB3), the autophagy inhibitor 3-MA, or the PI3K agonist 740 Y-P. Chondrocyte proliferation was evaluated using cell counting kit-8. Apoptosis was detected by flow cytometry. The levels of apoptosis-, extracellular matrix (ECM)-, autophagy-, and the PI3K/Akt/mTOR pathway-related proteins in chondrocytes were detected using immunoblotting or immunofluorescence.</p><p><strong>Results: </strong>We found that IL-1β stimulation upregulated miR-103-3p and downregulated CPEB3 in mouse chondrocytes. Inhibiting miR-103-3p reduced IL-1β-induced apoptosis and ECM macromolecule degradation while enhancing autophagy in chondrocytes. MiR-103-3p targeted CPEB3, and its downregulation rescued the expression of level in IL-1β stimulated-chondrocytes. MiR-103-3p downregulation inhibited the PI3K/Akt/mTOR pathway in IL-1β stimulated-chondrocytes by upregulating CPEB3. 3-MA, 740 Y-P, or CPEB3 knockdown counteracted the effect of miR-103-3p downregulation on chondrocyte apoptosis, ECM macromolecule degradation, and autophagy.</p><p><strong>Conclusion: </strong>Overall, inhibition of miR-103-3p reduces IL-1β-induced apoptosis and ECM macromolecule degradation in chondrocytes by enhancing autophagy through the CPEB3/PI3K/Akt/mTOR pathway.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"324"},"PeriodicalIF":2.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}