Journal of Orthopaedic Surgery and Research最新文献

{"title":"Pain mediator NGF improves chondrocyte extracellular matrix synthesis via PI3K/AKT pathway.","authors":"Mengling Wang, Jie Lian, Maoqing Ye, Bingchen An","doi":"10.1186/s13018-025-05503-x","DOIUrl":"10.1186/s13018-025-05503-x","url":null,"abstract":"<p><strong>Objectives: </strong>Nerve growth factor (NGF) is a key mediator in osteoarthritis pain signaling. Clinical studies revealed that anti-NGF antibodies are often accompanied by progressively worsening cartilage degeneration, although they exhibit significant analgesic effects. However, the relationship between NGF expression and cartilage destruction remains unclear. Our study aimed to investigate the effects of NGF on chondrocytes and to elucidate the underlying mechanisms involved.</p><p><strong>Methods: </strong>The ATDC5 cells were induced to differentiate into chondrocytes and stimulated with NGF at different concentrations (0.5-10 ng/mL). The cell counting kit-8 assay (CCK-8) was used to measure the effects of NGF on chondrocyte proliferation. Chondrocytes were subsequently stimulated with varying doses of NGF to identify the expression levels of the extracellular matrix. Chondrocytes were pretreated with GNF5837 (a tropomyosin receptor kinase A inhibitor) or LY294002 (a phosphoinositide 3-kinase inhibitor) before exposure to 5 ng/mL NGF to analyze associated signaling pathways. Western blotting and immunofluorescence staining were employed to analyze expression of related proteins.</p><p><strong>Results: </strong>Alcian blue, toluidine blue staining, and type II collagen immunofluorescence staining demonstrated that ATDC5 cells differentiated into functional chondrocytes after 14 days of chondrogenic induction. The CCK-8 assay confirmed that cell proliferation was unaffected. NGF (0.5-5 ng/mL) was found to enhance chondrocyte matrix synthesis in a dose-dependent fashion, particularly in the expression of aggrecan, type II collagen, Sox9, and through the activation of the PI3K/AKT signaling pathway. The highest promoting effects were exhibited at 5 ng/mL of NGF. Further analysis indicated that GNF5837 (TRKA inhibitor) or LY294002 (PI3K inhibitor) could reverse the protective effects of NGF on chondrocyte matrix synthesis.</p><p><strong>Conclusion: </strong>Our study identified a potentially beneficial role of NGF at concentrations of 0.5-5 ng/mL in chondrocytes, enhancing extracellular matrix synthesis, with significant involvement of the PI3K/AKT signaling pathway in this process.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"207"},"PeriodicalIF":2.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimally invasive surgery and conservative treatment achieve similar clinical outcomes in patients with type II fragility fractures of the pelvis.","authors":"Yaqi Zong, JingWei Li, Zhiyong Li, Wenzhi Wang","doi":"10.1186/s13018-025-05581-x","DOIUrl":"10.1186/s13018-025-05581-x","url":null,"abstract":"<p><strong>Objective: </strong>To compare clinical efficacy and quality of life (QoL) outcomes between minimally invasive surgery and conservative treatment for type II fragility fractures of the pelvis (FFP II).</p><p><strong>Methods: </strong>A total of 150 patients with FFP II, treated at our hospital from January 2019 to December 2022, were included in this study. The mean follow-up period was 22 ± 5 months. Patients were divided into two groups: 68 were assigned to the minimally invasive surgery group and 82 to the conservative treatment group. Clinical outcomes were assessed using the Majeed questionnaire and the self-reported Short Musculoskeletal Function Assessment. Health-related quality of life was evaluated with the Short-Form 36 Health Survey and World Health Organization Quality of Life Brief Version questionnaires.</p><p><strong>Results: </strong>There were no significant differences in basic information (gender/cause of injury/comorbidities/Rommens classification/osteoporosis status) or clinical outcomes between the two groups on the basis of the Majeed and Short Musculoskeletal Function Assessment questionnaire scores at the final follow-up. However, the minimally invasive surgery group showed a significant improvement in QoL compared with the conservative treatment group (including on the Short-Form 36 Health Survey and World Health Organization Quality of Life Brief Version; P < 0.01).</p><p><strong>Conclusion: </strong>Minimally invasive surgery and conservative treatment achieve similar clinical outcomes in patients with FFP II fractures. However, minimally invasive surgery significantly enhances the health-related QoL of these patients.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"210"},"PeriodicalIF":2.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MMP expression and its clinical significance in intervertebral disc destruction of spinal tuberculosis, Brucellar spondylitis, and pyogenic spondylitis.","authors":"Yuxin Gao, Xiaojun Ma, Zhiyun Shi, Mengqi Zhu, Zongqiang Yang, Zhengyong Tao, Ningkui Niu","doi":"10.1186/s13018-025-05622-5","DOIUrl":"10.1186/s13018-025-05622-5","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;This study is designed to investigate the roles of MMP-2, MMP-9, and MMP-13 in intervertebral disc destruction resulting from different types of spinal infections and their correlations with clinical quantitative data.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Disc tissue samples were collected from 60 patients with spinal infections (20 cases each of STB, BS, and PS in the infection group) and 20 patients with intervertebral disc herniation (control group). The expressions of MMP-2, MMP-9, and MMP-13 were detected by RT-qPCR. Correlation analysis was carried out with clinical quantitative data such as preoperative erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), interleukin-6 (IL-6), procalcitonin (PCT), and related blood routine indicators in the infection group.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In the analysis between the infection group and the control group, MMP-13 was expressed in the diseased intervertebral disc tissue of STB patients, but the result was not statistically significant (P = 0.2172). There was a significant difference in the expression of MMP-13 in the diseased intervertebral discs of BS and PS patients. The expressions of MMP-9 and MMP-2 were markedly increased in the diseased intervertebral disc tissue of STB, BS, and PS patients (all P &lt; 0.05). In the inter-group analysis of the infection group, the expression of MMP-13 in the diseased intervertebral disc tissue of PS patients was significantly different from that of STB and BS (P &lt; 0.0001), while there was no significant difference between the STB and BS groups (P = 0.2393). The expression of MMP-9 in the diseased intervertebral disc tissue of STB patients was significantly different from that of BS and PS (P &lt; 0.0001), but there was no statistically significant difference between the BS and PS groups (P = 0.9643). There was no statistically significant difference in the expression of MMP-2 among the STB, BS, and PS groups. In the correlation analysis with clinical quantitative data, MMP-13 was positively correlated with CRP, ESR, IL-6, WBC, and NEUT levels (r values were 0.7346, 0.3465, 0.3326, 0.6347, and 0.5152 respectively), and negatively correlated with LYM level (r = -0.5152, P &lt; 0.05), and had no correlation with PCT and MXD levels. MMP-9 was positively correlated with ESR level (r = 0.3412, P &lt; 0.05) and had no correlation with CRP, IL-6, PCT, WBC, NEUT, and LYM levels. MMP-2 was positively correlated with NEUT and LYM levels (r values were 0.3021 and 0.3306 respectively, P &lt; 0.05) and had no correlation with ESR, CRP, IL-6, PCT, and WBC levels.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;MMP-2, MMP-9, and MMP-13 play crucial roles in intervertebral disc destruction due to spinal infections. The differential expression of MMPs may be one of the reasons for the varying degrees of intervertebral disc destruction in different types of spinal infections. Moreover, when clinical indicators such as CRP, ESR, IL-6, WBC, and NEUT increase, it su","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"208"},"PeriodicalIF":2.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between dietary fiber intake and osteoarthritis: a cross-sectional study from the 1999-2018 U.S. National Health and Nutrition Examination Survey.","authors":"Xiaofeng Lv, Xinmin Deng, Rui Lai, Shanshan Liu, Zihao Zou, Xuechun Dai, Yalan Luo, Qiang Yuan, Ying Li","doi":"10.1186/s13018-025-05625-2","DOIUrl":"10.1186/s13018-025-05625-2","url":null,"abstract":"<p><strong>Objective: </strong>The relationship between dietary fiber intake and osteoarthritis (OA) remains unclear. This cross-sectional study, using data from the National Health and Nutrition Examination Survey (NHANES), aimed to examine the association between dietary fiber intake and OA.</p><p><strong>Methods: </strong>A cross-sectional analysis was conducted using NHANES data from 1999 to 2018 to assess the association between dietary fiber intake and OA. Univariate and multivariate weighted logistic regression models, along with restricted cubic spline (RCS) curves, were used to evaluate the relationship.</p><p><strong>Results: </strong>A total of 30,620 participants were included in this study, of whom 1,864 were diagnosed with OA, yielding a prevalence of 5.74%. Multivariate weighted logistic regression revealed a consistent inverse association between dietary fiber intake and OA (OR = 0.99, 95% CI: 0.97-0.99, P = 0.018). When dietary fiber was treated as a categorical variable, the highest quartile of intake (Q4) was associated with a 27% lower risk of OA compared to the lowest quartile (Q1) (OR = 0.73, 95% CI: 0.58-0.92, P = 0.007). The RCS analysis indicated a non-linear association between dietary fiber intake and OA risk (non-linear P = 0.013). The threshold effect interval suggested that dietary fiber intake in the range of 14.4-26.7 g was associated with a reduced risk of OA, while intake above this level did not provide significant additional protection.</p><p><strong>Conclusion: </strong>The findings demonstrate a negative linear association between dietary fiber intake and OA risk. Increasing dietary fiber consumption may reduce the risk of OA, offering potential strategies for its prevention and management. Further studies are needed to confirm these findings.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"209"},"PeriodicalIF":2.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the mechanism of Shuanghe decoction against steroid-induced osteonecrosis of the femoral head: insights from network pharmacology, metabolomics, and gut microbiota.","authors":"Kai Zhu, Wanxin Liu, Yuanyuan Peng, Xiaoqiang Wang, Zhenhao Wang, Jun Zheng, Guoying Deng, Qiugen Wang","doi":"10.1186/s13018-025-05619-0","DOIUrl":"10.1186/s13018-025-05619-0","url":null,"abstract":"<p><strong>Background: </strong>Steroid-induced osteonecrosis of the femoral head (SONFH) is a challenging and debilitating orthopedic condition with a rising incidence in recent years. Shuanghe Decoction (SHD), a traditional Chinese medicine formula, has shown significant efficacy in treating SONFH, though its underlying mechanisms remain unclear.</p><p><strong>Purpose: </strong>This study aims to elucidate the therapeutic effects and potential mechanisms of SHD on SONFH through in vivo experiments, combined with network pharmacology, metabolomics, and gut microbiota analysis.</p><p><strong>Materials and methods: </strong>Forty male Sprague-Dawley rats (300 ± 20 g) were randomly assigned to four groups: Control, Model, SHD-L, and SHD-H, with 10 rats each. SONFH was induced in all groups except the Control group using lipopolysaccharide and methylprednisolone. The SHD-L and SHD-H groups were treated with Shuanghe decoction at doses of 4.86 g/kg/day and 9.72 g/kg/day, respectively, for eight weeks. Bone morphology, pathological changes, and osteogenic factors were evaluated using Micro-CT, histological staining, and immunohistochemistry. Network pharmacology, metabolomics, and gut microbiota analyses were conducted to explore SHD's mechanisms.</p><p><strong>Results: </strong>SHD improved bone morphology and increased osteogenic factor expression (RUNX2, OCN, COL-I). Network pharmacology indicated that metabolic pathways play a key role in SHD's therapeutic effects. Metabolomic analysis identified 14 differential metabolites, including 21-hydroxypregnenolone and tyramine, which were restored to normal levels by SHD. Gut microbiota analysis revealed that SHD modulated bacterial abundance, particularly Verrucomicrobia, Allobaculum, and Burkholderiales. A comprehensive network identified two key metabolites (tyramine, 21-hydroxypregnenolone), seven targets (CYP19A1, CYP1A2, CYP1B1, CYP2C9, CYP3A4, MIF, and HSD11B1), two metabolic pathways (tyrosine metabolism, steroid hormone biosynthesis), and four bacterial taxa (Jeotgalicoccus, Clostridium, Corynebacterium, rc4-4) as central to SHD against SONFH.</p><p><strong>Conclusion: </strong>SHD alleviates SONFH by reshaping gut microbiota, reversing metabolic imbalances, and enhancing osteogenesis. Our findings provide novel insights into the pharmacological mechanisms of SHD, laying a foundation for its clinical application in treating SONFH.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"202"},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11863617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of chitosan/β-glycerophosphate/collagen hydrogel combined with MSCs on chronic achilles tendon injury via the Akt/GSK-3β pathway.","authors":"Songlin Liu, Liang Ma","doi":"10.1186/s13018-025-05607-4","DOIUrl":"10.1186/s13018-025-05607-4","url":null,"abstract":"<p><strong>Background: </strong>Chronic Achilles tendon injuries, commonly resulting from inadequate management of acute incidents, significantly reduce patients' quality of life. Current treatments, including conservative, surgical, and regenerative approaches, often yield suboptimal results. This study investigated the therapeutic effectiveness of a chitosan/β-glycerophosphate/collagen (C/GP/Co) hydrogel combined with bone marrow mesenchymal stem cells (MSCs) for chronic Achilles tendon injury in a rat model.</p><p><strong>Material & methods: </strong>A temperature-sensitive injectable C/GP/Co hydrogel was synthesized and combined with MSCs to treat a chronic Achilles tendon injury in Sprague-Dawley rats. The rats were divided into four groups receiving saline (model), C/GP/Co hydrogel, C/GP/Co/MSCs hydrogel, or normal control. After 6 weeks, morphological, biomechanical, and molecular assessments were conducted, including histology, Western blot analysis for protein expression, and the evaluation of the Akt/GSK-3β signaling pathway.</p><p><strong>Results: </strong>The C/GP/Co/MSCs hydrogel significantly enhanced tendon healing compared to the model and C/GP/Co groups, as evidenced by improved collagen fiber organization and an increased type I/III collagen ratio on histological analysis. Western blot results revealed activation of the Akt/GSK-3β pathway by the C/GP/Co/MSCs hydrogel, leading to enhanced tendon cell proliferation and reduced apoptosis, demonstrated by a decreased Bax/Bcl-2 ratio and Caspase-3 expression. Downregulation of inflammation markers CD206 and CD163 was significant. Biomechanical testing indicated that the C/GP/Co/MSCs hydrogel restored tendon tensile strength closer to normal levels.</p><p><strong>Conclusions: </strong>The C/GP/Co/MSCs hydrogel establishes a supportive microenvironment for MSC function, aiding tendon healing through the Akt/GSK-3β pathway. Its dual role in inflammation and apoptosis reduction, while enhancing biomechanical properties, demonstrates its potential as an innovative treatment for persistent Achilles tendon ailments. Future research endeavors should comprehensively explore the molecular pathways and assess their clinical applicability.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"204"},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of platelet-rich plasma use as an adjunctive treatment in autologous osteochondral transplantation for patients with osteochondral lesions of the talus accompanied by chronic lateral ankle instability.","authors":"Jie Xiao, Xiu Yang, Yun Zheng, Shun Lin, Xue-Song Han","doi":"10.1186/s13018-025-05613-6","DOIUrl":"10.1186/s13018-025-05613-6","url":null,"abstract":"<p><strong>Background: </strong>Autologous osteochondral transplantation (AOT) combined with biological agents is an advanced technique for treating osteochondral lesions. Therefore, this study aimed to explore the effect of combining platelet-rich plasma (PRP) treatment with AOT on postoperative functional and magnetic resonance imaging (MRI) outcomes in patients with osteochondral lesions of the talus (OLTs) accompanied by chronic lateral ankle instability (CLAI).</p><p><strong>Methods: </strong>This retrospective study had a minimum follow-up period of 1 year. Thiry-nine patients with CLAI who underwent AOT between 2019 and 2023 were included in this study. Of these, 21 and 18 received AOT combined with PRP treatment (AOT + PRP group) and AOT alone (AOT-alone group), respectively. Preoperative and postoperative follow-up assessments were performed using the visual analog scale (VAS), American Orthopedic Foot and Ankle Society (AOFAS), and foot and ankle ability measure-sport scale (FAAM-sport scale). The final follow-up MRI was evaluated using the Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) 2.0 ankle scoring system.</p><p><strong>Results: </strong>Both groups showed a significant reduction in VAS scores and significant improvements in AOFAS and FAAM-sport scale scores at the final follow-up compared with the preoperative values. No significant differences were observed in the final follow-up VAS, AOFAS, FAAM-sport scale, and MOCART 2.0 ankle scores between the groups. However, significant between-group differences were found at postoperative months 1 (P < 0.001) and 3 (P = 0.031) for VAS scores and at postoperative month 3 for FAAM-sport scale scores (P = 0.005). The AOT + PRP group showed significantly better final follow-up scores for the \"surface of the repair tissue\" on the MOCART 2.0 ankle score system than the AOT-alone group (P = 0.029).</p><p><strong>Conclusions: </strong>PRP did not result in significantly superior outcomes when used as an adjunct to AOT compared to AOT alone in the setting of concomitant OLTs and CLAI.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"203"},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cisplatin-induced oxidative stress, apoptosis, and pro-inflammatory responses in chondrocytes through modulating LOX-1.","authors":"Chin-Hsien Wu, Wan-Ching Chou, I-Ming Jou, Yuan-Kun Tu, Ching-Hou Ma, Kun-Ling Tsai","doi":"10.1186/s13018-025-05602-9","DOIUrl":"10.1186/s13018-025-05602-9","url":null,"abstract":"<p><p>Cisplatin is a potent and efficacious anticancer medication. In pediatric cancer, the height of the growth plate's proliferating layer is known to be reduced by cisplatin, but researchers have not yet determined the specific mechanism behind this phenomenon. Lectin-like oxidized low-density lipoprotein receptor-1 is known to be involved in the development of osteoarthritis and atherosclerosis. The equilibrium of cartilage is regulated by LOX-1, but the function of LOX-1 in cisplatin-induced chondrocyte impairment remains unknown. Positive regulation of LOX-1 leads to increased cellular oxidative stress and cell damage. Research has shown that blocking of LOX-1 can reduce the chondrocyte damage and oxidative stress in cells induced by oxidized LDL treatment. However, the role of LOX-1 in cisplatin-mediated chondrocyte damage is still unclear. This study found that cisplatin increased ROS concentration and p38, ERK phosphorylation. Cisplatin activated NF-κB in chondrocytes. In addition, LOX-1 small interfering RNA transfection mitigated cisplatin-induced apoptosis in TC28a2 cells. Phosphorylated extracellular signal-regulated kinase and p38 were dose-dependently increased by administration of cisplatin. Silencing LOX-1 or MAPK inhibition reduces cisplatin-caused apoptosis. The findings suggest that cisplatin-induced growth plate dysfunction operates through the LOX-1/p38/NF-κB signaling pathway.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"206"},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel technique of intraoperative infiltration between posterior capsule and popliteal artery can reduce opioid consumption and blood loss in total knee arthroplasty surgery: an age- and gender-matched study.","authors":"Hsuan-Hsiao Ma, Shun-An Kan, Fang-Yao Chiu, Yu-Ping Su","doi":"10.1186/s13018-025-05605-6","DOIUrl":"10.1186/s13018-025-05605-6","url":null,"abstract":"<p><strong>Purpose: </strong>Interspace infiltration between the posterior knee capsule and popliteal artery (iPACK) is an effective method for pain control following total knee arthroplasty (TKA). However, this procedure still requires an experienced physician to perform ultrasound-guided injections postoperatively, and the patient will need to undergo additional injections aside from the surgery. This study investigates a novel, simplified intraoperative iPACK (I-iPACK) technique, applied during surgery without the need for ultrasound or an anesthesiologist. We hypothesized that combining I-iPACK with periarticular injection (PAI), comparing with using PAI alone, could reduce postoperative morphine consumption and improve the range of motion (ROM) in patients after TKA.</p><p><strong>Methods: </strong>This retrospective analysis reviewed data from 600 patients who underwent unilateral TKA, selected from an initial pool of 3502. The patients were divided into two groups: 300 received PAI with I-iPACK (Group A), and 300 received PAI alone (Group B). The groups were matched by age and gender. The primary outcome parameters postoperative pain levels (VAS scores) and cumulative morphine consumption at 24, 48, and 72 h. The secondary outcome parameters were maximum tolerated ROM measured by continuous passive motion (CPM) through time and adverse events, such as nausea, dizziness, constipation, and gastrointestinal bleeding, monitored for two weeks post-surgery. The hypothesis is the additional I-iPACK technique is not inferior to the PAI alone. Non-parametric statistical methods were used for analysis, with a significance threshold set at p < 0.05.</p><p><strong>Results: </strong>Both groups were comparable in demographics, and no significant differences in surgery time were noted. However, the I-iPACK group had significantly less blood loss. Patients receiving I-iPACK had improved ROM at 24 h, reduced morphine consumption at all measured time points, and had lower rates of complications including constipation and dizziness. The two groups showed no significant differences in other complications or pain scores.</p><p><strong>Conclusion: </strong>The addition of I-iPACK to PAI during TKA surgery effectively reduces morphine consumption, limits blood loss, and enhances early postoperative ROM without increasing adverse events such as nerve palsy. Similar technique of intra-operative infiltration for pain control could be adopted in diverse surgical settings. However, the lack of blinding might have minor impacts on the patient-reported outcome such as VAS scores.</p><p><strong>Level of evidence: </strong>Level III, Therapeutic Study.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"205"},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between age, gender, ulnar variance and triangular fibrocartilage disc on high-resolution MRI in asymptomatic adults' wrist.","authors":"Huili Zhan, Zhanhua Qian, Jianing Cui, Ping Wang, Rongjie Bai, Yuming Yin","doi":"10.1186/s13018-025-05618-1","DOIUrl":"10.1186/s13018-025-05618-1","url":null,"abstract":"<p><strong>Background: </strong>Incidental triangular fibrocartilage complex (TFCC) abnormal findings could be detected on magnetic resonance imaging (MRI), whether these incidental findings were causes of symptoms can confuse clinicians. The purpose of this study was to demonstrate the MRI features of triangular fibrocartilage (TFC) disc in asymptomatic adults' wrists and explore the association with age, gender and ulnar variance.</p><p><strong>Methods: </strong>Healthy adult volunteers who reported no ulnar-sided wrist pain were recruited and underwent MRI examinations. The thickness and angle of TFC and ulnar variance were measured on the mid-coronal slice, and coronal morphology and signal characteristics of the TFC disc were assessed. Regression analyses were performed to evaluate the relationships between the MRI features of TFC and age, gender and ulnar variance.</p><p><strong>Results: </strong>Eighty-four asymptomatic adult volunteers (42 men, 42 women; mean age of men and women, 37 years and 46 years, respectively) were included, with 23 participants having a TFC defect. Ulnar variance was the influencing factor significantly associated with thickness, angle and morphology of TFC (all p < 0.001). The angles and prevalence of a more distally stretched disc were higher in women compared to men. Age was not a statistically significant factor of TFC thickness and angle, but age had a mediating effect [12.43% (p = 0.016)] on the association between ulnar variance and TFC morphology, while the direct effect of ulnar variance accounted for 87.57% (p < 0.001). Additionally, multivariate logistic regression results showed that an increase in age was significantly associated with an increased prevalence of abnormal TFC signal and discontinuity.</p><p><strong>Conclusions: </strong>Ulnar variance is the most significant factor in determining TFC thickness, angle, and morphology. Age did not affect thickness and angle directly but may influence the relationship between ulnar variance and TFC morphology, and age is associated with an increased prevalence of abnormal signal and discontinuity in the TFC. This study offers a reference for clinicians and a comprehensive consideration and analysis, including age, ulnar variance, clinical history and physical examination should be made when interpreting MRI findings.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"201"},"PeriodicalIF":2.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11863456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}