Journal of Orthopaedic Surgery and Research最新文献

{"title":"Outcomes of cemented and uncemented stem fixation in Vancouver B1 periprosthetic femur fractures treated with locking plates.","authors":"Sezer Astan, Orhan Balta","doi":"10.1186/s13018-025-05979-7","DOIUrl":"10.1186/s13018-025-05979-7","url":null,"abstract":"<p><strong>Background: </strong>Vancouver type B1 periprosthetic femoral fractures (PFFs) occur in elderly patients with well-fixed femoral stems and present challenges in treatment due to variations in bone quality and fixation methods. This study compared the clinical and radiological outcomes of cemented versus uncemented femoral stems in B1 PFFs treated with open reduction and internal fixation (ORIF) using locking compression plates.</p><p><strong>Materials and methods: </strong>This retrospective analysis included 41 (28 females and 13 males) patients who were treated between 2008 and 2020. Patients were categorized based on femoral stem fixation type. Data on fracture configuration, union time, complications, and Harris Hip Scores (HHS) were analyzed.</p><p><strong>Results: </strong>Transverse fractures were more common in the cemented group, while long oblique fractures predominated in the uncemented group. The cemented group demonstrated longer union times, greater blood loss, and significantly lower HHS (p < 0.001). The mean union time was found to be 23.22 ± 11.25 weeks in the cemented group and 16.28 ± 4.16 weeks in the uncemented group. Nonunion occurred exclusively in patients with cemented stems. Transverse fractures that had previously undergone cemented femoral stem fixation were observed to have a higher risk of nonunion (Odds ratio 2.4; 95% CI: 1.115-5.05, p = 0.02), indicating that the nonunion rate was 2.4 times higher in cases with cemented stems. The uncemented group showed earlier mobilization and better functional outcomes. In the cemented group, significantly longer healing time for PFF (p = 0.017), and increased blood loss (p < 0.001) were observed. The mean blood loss was 381.3 ± 123.48 mL in the cemented group and 218.33 ± 73.5 mL in the uncemented group.</p><p><strong>Conclusions: </strong>Vancouver type B1 PFFs with cemented femoral stems, particularly transverse fractures, are associated with poorer outcomes and increased nonunion risk following unilateral plate fixation. Stem revision may be considered in such cases. Further multicenter studies are needed to guide optimal treatment strategies.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"641"},"PeriodicalIF":2.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subtrochanteric valgus osteotomy in nonunited femoral neck fractures in children: two different fixation methods.","authors":"Mahmoud Badawy, Sami Ibrahim Sadek, Ahmed Mohamed Abdelwahab, Ibrahim Abdellatif Algohiny","doi":"10.1186/s13018-025-06051-0","DOIUrl":"10.1186/s13018-025-06051-0","url":null,"abstract":"<p><strong>Introduction: </strong>Nonunion of pediatric femoral neck fractures is a difficult situation for orthopedic surgeons. It can result in devastating results if left untreated. The purpose of the present study was to assess and compare the results of the treatment of nonunited pediatric femoral neck fractures by closed reduction and subtrochanteric valgus osteotomy with two different fixation methods.</p><p><strong>Patients and methods: </strong>Seventeen patients with nonunion femoral neck fractures were treated with closed reduction, subtrochanteric osteotomy and fixation with either the proximal humerus internal locking system (PHILOS plate) (group A) (10 patients) or the Wagner technique (group B) (7 patients). The mean age of the patients was 7 years, and the mean follow-up period was 16 months.</p><p><strong>Results: </strong>At the end of the follow-up, all patients, the fractures were united. We did not observe any cases of implant failure. There was a statistically significant improvement in the neck shaft angle in both groups from 88.70 ± 3.36 to 129.35 ± 7.34 in group A and from 87 ± 1.91 to 126.78 ± 4.06 in group B. Regarding the clinical outcome and according to the Ratliff concept, half of the cases were good in group A and 57.1% were good in group B. There was no statistically significant difference between the groups regarding time to union, degree of coxa vara correction or the Ratliff concept.</p><p><strong>Conclusion: </strong>Both the PHILOS plate and the Wagner technique offer good stable fixation options for nonunited pediatric femoral neck fractures.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"640"},"PeriodicalIF":2.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a glycolysis-related diagnostic model for osteoarthritis through integrated bioinformatics analysis and machine learning.","authors":"Wangnan Mao, Zhengsheng Bao, Bingbing Zhang, Lianguo Wu","doi":"10.1186/s13018-025-06072-9","DOIUrl":"10.1186/s13018-025-06072-9","url":null,"abstract":"<p><strong>Background: </strong>Osteoarthritis (OA) is a prevalent degenerative joint disease that significantly contributes to global disability. Glycolysis, a fundamental process in cellular energy metabolism, is particularly vital for chondrocytes in OA. This study aims to explore the intrinsic relationship between glycolysis-related genes (GRGs) and OA.</p><p><strong>Methods: </strong>We incorporated three publicly available datasets from the Gene Expression Omnibus (GEO) database, which included 64 OA samples and 34 normal controls. By utilizing differential expression analysis, weighted gene co-expression network analysis, protein-protein interaction networks, and machine learning methods, we identified three diagnostic biomarkers of OA patients. The expression levels of these biomarkers were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemical (IHC). Additionally, a competing endogenous RNA (ceRNA) network was constructed to explore potential regulatory interactions.</p><p><strong>Results: </strong>Through bioinformatics and machine learning approaches, three glycolysis-related biomarkers-HMGB2, SLC7A5, and ADM-were identified. The diagnostic model based on these GRGs demonstrated high predictive accuracy, with an AUC of 0.92 in the training set and 0.85 in the validation set. Subsequently, qRT-PCR and IHC confirmed the differential expression of hub genes in human cartilage samples. Furthermore, immunocyte infiltration analysis revealed distinct immune cell infiltration profiles between OA and HC groups. Notably, lncRNA XIST was found to regulate all three biomarkers, indicating its potential as a therapeutic target for OA.</p><p><strong>Conclusion: </strong>This study provides novel insights into the role of glycolysis in OA pathogenesis and highlights its potential as a target for diagnosis, prevention, and treatment strategies.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"639"},"PeriodicalIF":2.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring gender disparities in academic orthopaedic surgery faculty: analyzing subspecialty and leadership diversity to foster inclusivity.","authors":"Malini Anand, Kaitlyn R Julian, Mary K Mulcahey, Stephanie E Wong","doi":"10.1186/s13018-025-06048-9","DOIUrl":"10.1186/s13018-025-06048-9","url":null,"abstract":"<p><strong>Background: </strong>The historic gap in gender diversity within orthopaedic surgery is widely acknowledged and continues to persist. The lack of female representation in orthopaedic surgery has been attributed to a variety of factors, including the absence of female mentors and leaders within the field. As such, we sought to examine the gender diversity among orthopaedic surgery faculty in various subspecialties at academic institutions and the distribution of female faculty in positions of leadership.</p><p><strong>Methods: </strong>The American Medical Association Fellowship and Residency Electronic Interactive Database (FREIDA) was used to identify all allopathic orthopaedic surgery residency programs during the 2022 to 2023 academic year. The total number of faculty, and distribution of female faculty by subspecialty were collected from January to March 2023. The mean and percentage of female faculty in each subspecialty per program was calculated.</p><p><strong>Results: </strong>The total number of orthopaedic surgery female faculty identified was 524. The subspecialty with the highest percentage of female faculty per program was pediatrics at 26.1% (148/511). Hand (18.6%; 113/511), oncology (19.2%; 38/511), foot and ankle (13.6%; 49/511), spine (3.9%; 21/511), shoulder and elbow (7.4%; 7/511) and adult reconstruction (3.7%; 24/511) had lower percentages of female faculty per program. A total of 52 (10.2%) female section chiefs were identified across all programs. Oncology had the highest percentage of female faculty represented in section leadership at 18.4% (7) and sports medicine had the lowest at 4.8% (4).</p><p><strong>Conclusion: </strong>Gender diversity of faculty in orthopaedic surgery is low with adult reconstruction (3.7%), spine (3.9%), and shoulder and elbow (7.4%) having the lowest percentages of female faculty. The percentage of female faculty represented in section leadership is also lacking with a total of 52 (10.2%) female section chiefs identified across all programs. Increasing the number of females in leadership positions across all orthopaedic subspecialties may be one step in helping improve gender diversity in the field.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"638"},"PeriodicalIF":2.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cartilage-protective effects of lopinavir/ritonavir: in vitro and in silico exploration of the HIF-1α/SOX9/IL-1β pathway.","authors":"Hanefi Ozbek, Ibrahim Yilmaz, Aslı Akyuz, Suray Pehlivanoglu, Duygu Yasar Sirin, Mehmet Albayrak, Omer Faruk Yilmaz","doi":"10.1186/s13018-025-06068-5","DOIUrl":"10.1186/s13018-025-06068-5","url":null,"abstract":"","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"642"},"PeriodicalIF":2.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in unilateral endoscopic spinal surgery for the treatment of lumbar disc herniation: a review.","authors":"Nan Fang, Shilin Yan, Aofei Yang","doi":"10.1186/s13018-025-06071-w","DOIUrl":"10.1186/s13018-025-06071-w","url":null,"abstract":"<p><p>Lumbar disc herniation is a common cause of back and leg pain. Treatment has evolved significantly from traditional open surgery towards minimally invasive spine surgery (MISS). Within MISS, unilateral endoscopic spinal surgery (UESS), such as percutaneous transforaminal endoscopic discectomy (PTED) and unilateral biportal endoscopy (UBE), has largely replaced older bilateral approaches. Recent innovations like one-hole split endoscopy (OSE) and arthroscopic-assisted uniportal surgery (AUSS) further expand UESS capabilities. This review provides the first comprehensive analysis of UESS evolution and its key surgical approaches. We systematically compare the technical features of major uniportal techniques, including established methods like TESSYS and interlaminar approaches, alongside newer developments such as the 45° puncture technique and Gu's technique. Looking ahead, intelligent solutions and instrument innovation represent crucial future directions for UESS. Notably, this study formulates conceptual designs for a novel integrated non-coaxial spinal endoscope as potential future directions. These insights provide critical guidance for developing next-generation endoscopic devices in UESS.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"643"},"PeriodicalIF":2.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D deficiency and adverse body composition in adolescents.","authors":"Bo Wang, Songting Gao, Zhongxin Zhu","doi":"10.1186/s13018-025-06091-6","DOIUrl":"10.1186/s13018-025-06091-6","url":null,"abstract":"<p><strong>Background: </strong>Vitamin D deficiency is a global public health concern, particularly among adolescents. While its skeletal effects are well-established, evidence on its extraskeletal roles in muscle and adipose tissue during this critical developmental period remains limited, especially using direct body composition measures.</p><p><strong>Methods: </strong>We analyzed 3,648 participants aged 12-19 years from NHANES data (2011-2018), stratifying vitamin D status as deficiency, insufficiency, or sufficiency. Multivariable linear regression models evaluated associations with appendicular lean mass index (ALMI), visceral adipose tissue area (VATA), and total bone mineral density (BMD). Subgroup analyses explored age, sex, and racial/ethnic variations.</p><p><strong>Results: </strong>Vitamin D deficiency/insufficiency affected 40.1% of participants. Higher serum vitamin D status correlated with positive body composition: lower VATA (β = -0.092, 95% CI: -0.124 to -0.061), and higher ALMI (β = 0.005, 95% CI: 0.004 to 0.006) and BMD (β = 0.0008, 95% CI: 0.0006 to 0.0010). Subgroup analyses revealed stronger ALMI associations in older boys and Mexican Americans, while VATA reductions were most pronounced in younger girls. BMD benefits were consistent across subgroups.</p><p><strong>Conclusions: </strong>Vitamin D deficiency is prevalent among adolescents and independently associated with unfavorable body composition, modulated by demographic factors. These findings highlight the need for targeted interventions to optimize musculoskeletal and metabolic health during this developmental window.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"637"},"PeriodicalIF":2.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex differences of proximal tibia anthropometry in a Chinese population: comparison with sizing of tibial implants.","authors":"Bo Yang, Ming Chen, Xiang-Hui Huang, Shizhang Liu, Yanhai Chang, Peng-Zhen Lei","doi":"10.1186/s13018-025-06020-7","DOIUrl":"10.1186/s13018-025-06020-7","url":null,"abstract":"<p><strong>Background: </strong>Matching tibial component with resected tibia dimensions is essential for good clinical outcomes in total knee arthroplasty.The aim of this study was to determine the sex differences in anthropometry at the surface of the resected proximal tibia in Chinese population and compare the anthropometric values with the dimensions of available tibial components currently used in China.</p><p><strong>Methods: </strong>The anthropometric characteristics of the proximal tibias in 241 Chinese individuals (121 males, 120 females) were assessed via computed tomography imaging. We measured the mediolateral (ML), anteroposterior (AP), medial and lateral anteroposterior dimensions and the ML/AP aspect ratio of the resected proximal tibial surface. The ML/AP aspect ratios were classified into 3 groups on the basis of the AP dimensions (< 48 mm, 48-51 mm, > 51 mm) to compare sex differences. All the anthropometric data were compared with the dimensions of the six tibial components currently used.</p><p><strong>Results: </strong>The tibias ML and AP dimensions of females were significantly smaller than that of males (69.5 ± 3.4 mm, 46.7 ± 2.3 mm vs. 78.4 ± 3.2 mm, 52.6 ± 2.9 mm ). We found females had a smaller ML/AP aspect ratio than males in the subgroups (< 48 mm P = 0.009; 48-51 mm P < 0.001; >51 mm P = 0.043). Compared with component sizes, there was a mismatch between tibial morphology and the dimensions of all the implants. Tibial components with smaller AP measurements were undersized in the ML dimension, and those with larger AP measurements were oversized in the ML dimension in males and females.</p><p><strong>Conclusion: </strong>In the Chinese population, females have smaller dimensions and ML/AP aspect ratios than their male counterparts. The results of this study can provide fundamental data on designing optimal tibial components, especially for design of sex-specific prostheses with different ML/AP aspect ratios for the Chinese males and females.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"632"},"PeriodicalIF":2.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12243352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pelvic, trunk and upper limb biomechanics during walking in individuals with spinal deformities: a systematic review and meta-analysis.","authors":"Fateme Khorramroo, Reza Rajabi, Seyed Hamed Mousavi","doi":"10.1186/s13018-025-06063-w","DOIUrl":"10.1186/s13018-025-06063-w","url":null,"abstract":"<p><strong>Background: </strong>Spinal deformities represent a major public health concern, characterized by abnormal curvature and alignment of the spine. We aimed to investigate the alterations in pelvic, trunk, and upper limb biomechanics during walking in individuals with spinal deformities compared to healthy controls.</p><p><strong>Methods: </strong>We searched four databases including PubMed, Web of Science, Scopus and Embase from their inception through 2nd january 2025. Two authors screened studies and separately extracted data from included studies. The Newcastle-Ottawa Scale was applied to assess quality of included studies. GRADE was employed to assess the overall quality of the evidence in the meta-analysis. Subgroup and sensitivity analyses were performed to address potential heterogeneity. Mean differences and 95% confidence intervals (CI) were calculated with random effects model in RevMan version 5.4.</p><p><strong>Results: </strong>Seventeen studies with a total of 893 participants were included in the systematic review. In scoliosis, meta-analysis showed strong evidence of increased thorax-pelvis sagittal ROM, strong evidence of non-significant increase in SVA, moderate evidence of increased pelvic frontal ROM, sagittal thoracic curve ROM, CVA, and moderate evidence of non-significant change in 3-dimensional thorax movement, sagittal lumbar ROM, thorax-pelvis frontal and transverse ROM compared to controls during walking. The meta-analysis for other variables related to scoliosis indicated moderate evidence of non-significant changes. Meta-analysis showed moderate evidence of increased sagittal pelvic ROM in sagittal trunk malalignment, and moderate evidence of non-significant increase in pelvic ROM in adult spinal deformities compared to controls during walking.</p><p><strong>Conclusion: </strong>This review highlights the complexity of pelvic, trunk and upper limb biomechanics in individuals with spinal deformities. Understanding these alterations is essential for creating targeted interventions and improving clinical outcomes.</p><p><strong>Trial registration: </strong>The protocol was registered with PROSPERO (CRD42024602489).</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"636"},"PeriodicalIF":2.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12243263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epimedium Brevicornu and Curculigo orchioides inhibit osteoclast autophagy by degrading the level of miRNA-199 to regulate the mTOR signaling pathway.","authors":"Mengyao Shi, Peng Sun, Fang Chai","doi":"10.1186/s13018-025-06043-0","DOIUrl":"10.1186/s13018-025-06043-0","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect and mechanism of Epimedium brevicornu Maxim. and Curculigo orchioides Gaertn. (EBCO) on miRNA-199 expression in osteoclasts.</p><p><strong>Methods: </strong>EBCO-containing and blank serum was drawn from Wistar rats received EBCO (12 g/kg/d) or the same volume of dH₂O for 7 days intragastrically. RAW264.7 cells were induced to differentiate into osteoclasts. The miRNA-199 and Mtor mRNA level of osteoclasts treated with blank serum and low (5%), medium (10%), high (20%) concentrations of EBCO-containing serum for 48 h were measured. The binding relationship between miRNA-199 and Mtor was verified by dual-luciferase assay. Osteoclasts were divided into control, blank serum, EBCO-containing serum, blank serum, or EBCO-containing serum with 2 µM MHY1485 (mTOR agonist) or 10 nM Dactolisib (mTOR inhibitor) groups, proliferation, ROS level, miRNA-199 mRNA level, the expression of mTOR signaling pathway and autophagy-related proteins expression were observed.</p><p><strong>Results: </strong>Compared with the control group, EBCO-containing serum significantly (P < 0.05) decreased the level of miRNA-199 in osteoclasts, increased Mtor mRNA level. The relative fluorescence level was significantly (P < 0.01) decreased after co-transfection of miRNA-199 mimics and MTOR-3'UTR-WT compared to NC-mimics group. EBCO-containing serum reduced cell viability and ROS level, suppressed light chain 3 (LC3)-II/LC3-I and Beclin-1, enhanced phosphorylated mTOR, mTOR and phosphorylated p70S6 kinase (p-p70S6K) proteins expression.</p><p><strong>Conclusions: </strong>EBCO delays the progression of osteoporosis by reducing miRNA-199 in osteoclasts to regulate mTOR signaling and inhibit autophagy. Further research is needed to explore new avenues of clinical application.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"631"},"PeriodicalIF":2.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12243131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}