Journal of oleo science最新文献

{"title":"The Potential of Roselle (Hibiscus sabdariffa) Plant in Industrial Applications: A Promising Source of Functional Compounds","authors":"Lye Yee Chew, Suk Kuan Teng, Yun Ping Neo, Yan Yi Sim, Sook Chin Chew","doi":"10.5650/jos.ess23111","DOIUrl":"https://doi.org/10.5650/jos.ess23111","url":null,"abstract":"</p><p>Roselle is an annual botanical plant that widely planted in different countries worldwide. Its different parts, including seeds, leaves, and calyces, can offer multi-purpose applications with economic importance. The present review discusses the detailed profile of bioactive compounds present in roselle seeds, leaves, and calyces, as well as their extraction and processing, to explore their potential application in pharmaceutical, cosmetic, nutraceutical, food and other industries. Roselle seeds with high phenolics, fiber, and protein contents, which are suitable to use in functional food product development. Besides, roselle seeds can yield 17-20% of roselle seed oil with high content of linoleic acid (35.0-45.3%) and oleic acid (27.1- 36.9%). This unique fatty acid composition of roselle seed oil makes it suitable to use as edible oil to offer the health benefits of essential fatty acid. Moreover, high contents of tocopherols, phenolics, and phytosterols were detected in roselle seed oil to provide nutritional, pharmaceutical, and therapeutic properties. On the other hand, roselle leaves with valuable contents of phenols, flavonoids, organic acid, and tocopherols can be applied in silver nanoparticles, food product development, and the pharmaceutical industry. Roselle calyces with high content of anthocyanins, protocatechuic acids, and organic acids are widely applied in food and colorant industries.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/3/73_ess23111/figure/73_ess23111.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"99 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of Nervonic and Erucic Acids in Human Milk: Comparison with Infant Formula with Different Fat Sources and Nutritional Stages","authors":"Beibei Duan, Jung-Ah Shin, Ki-Teak Lee","doi":"10.5650/jos.ess23146","DOIUrl":"https://doi.org/10.5650/jos.ess23146","url":null,"abstract":"</p><p>This study aims to quantitatively analyze nervonic (C24:1 n-9) and erucic (C22:1 n-9) acids in human milk (HM) collected from Korean mothers, along with the infant formulas (IFs) with different fat sources and nutritional stages. The content of nervonic and erucic acids in HM from Korean mothers were 2.13 and 2.81 mg/100 g, accounting for 0.06 and 0.08% of the total fatty acids (FAs), respectively. These acids in IFs based on fat from plant-oil formula were higher than those in IFs based on cow milk formula. The composition of erucic acid in IFs based on cow milk formula and plant-oil formula increased with the nutritional stages, while nervonic acid only increased in IFs based on the plant-oil formula.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/3/73_ess23146/figure/73_ess23146.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"5 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Selenium Nanoparticles and Their Effect on Pancreatic Functions and Acute Pancreatitis in Rats","authors":"Juan Shu, Yusheng Liao, Jian Wang, Yuanjie Zhang, Weilai Zhou, Heng Zhang","doi":"10.5650/jos.ess23190","DOIUrl":"https://doi.org/10.5650/jos.ess23190","url":null,"abstract":"</p><p>Acute pancreatitis (AP) have been documented to have severe impact on pancreatic function. Frequent incidence of AP can result in chronic pancreatitis and thereby it can increase the probability of pancreatic cancers. This study intended to examine the effect of selenium nanoparticles (Se-NPs) synthesized from <i>Coleus forskohlii</i> leaf extract on pancreatic function and AP in rat. Primarily, Se-NPs was fabricated using the <i>C. forskohlii</i> leaf extract. The synthesized nanomaterial was characterized through UV-visible, XRD, and FTIR spectroscopies. Notably, the zeta potential of Se-NPs was found to be –32.8 mV with a polydispersity index (PDI) of 0.18. Morphological analysis on SEM unveiled the spherical shape of Se-NP with an average particle size of 12.69 nm. Strikingly, cytotoxicity analysis on pancreatic cancer and normal cells unveiled the concentration-dependent toxicity profile. However, IC ₅₀ value is lower in normal pancreatic cell lines in comparison to pancreatic cancer cells lines. Assessment of Se-NPs on AP rats revealed the positive impact of Se-NPs. It effectively decreased the amount of lipase, amylase, IL-1β, MDA, NO, and Bcl-2 while increased the glucose, insulin, HOMA-β and antioxidant potential in AP rats. In addition, an evaluation of Se-NPs in the pancreatic functions revealed the non-harmful effect of Se-NPs.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/3/73_ess23190/figure/73_ess23190.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"60 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medium-Chain Triacylglycerols (MCTs) and Their Fractions in Drug Delivery Systems : A Systematic Review","authors":"Mohd Hanif Zulfakar, Hariny Pubadi, Salizatul Ilyana Ibrahim, Nuriana Munirah Hairul","doi":"10.5650/jos.ess23204","DOIUrl":"https://doi.org/10.5650/jos.ess23204","url":null,"abstract":"</p><p>Medium-chain triacylglycerol (MCT) is a type of triacylglycerol that has six or seven to twelve carbon chains. It consists of three molecules of fatty acids attached to one molecule of glycerol. Drug delivery system (DDS) is defined as a formulation to distribute drugs into the human body. The unique properties of MCTs have garnered interest in using them as excipients in DDS. Even though there are many significant effects attributed to the use of MCTs, especially in modulating the rate of drug delivery in various DDS, they are all limited and intermittent. This warrants a detailed summary of the previous studies on the use of MCTs in various DDS. Therefore, this review focuses on presenting a systematic review of previous studies on the use of MCTs in the last six years and explores the types and effects of MCTs on DDS that employ various types of delivery routes. A systematic search through PubMed, Science Direct and Scopus was performed. Keywords like “medium-chain triglycerides”, “medium-chain fatty acids”, “medium-chain triglycerides and their fractions”, “medium-chain fatty acids and their fractions”, “MCTs”, “MCFA”, “in drug delivery”, “in drug delivery system” and their combinations were used. The synonyms of the words were also used to extend the search. A total of 17 articles that met the inclusion criteria were identified. Findings from this review have identified the several MCTs and their fractions used in DDS that employed the oral/enteral, topical, transdermal, parenteral, and pulmonary routes of drug delivery. The review also highlights that the usage of MCTs in DDS results in a better transportation of drugs into the human body.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/3/73_ess23204/figure/73_ess23204.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"15 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Carrier Nanostructured Astilbin Ameliorates Rotenone-Induced Neurodegeneration in Mice Brain via Modulation of GSK3β-Nrf2 Signaling Pathways","authors":"Wenyuan Xu, You Dai","doi":"10.5650/jos.ess23173","DOIUrl":"https://doi.org/10.5650/jos.ess23173","url":null,"abstract":"</p><p>Astilbin is a flavanonol, found in St John’s wort (<i>Hypericum perforatum</i>) and many other plants. It has been demonstrated that astilbin contains anti-inflammatory, antioxidant, and immune-suppressive properties. However, the bioavailability of astilbin remains a question for which drug delivery-based nanoparticles can be utilized. We formulated a nanostructured lipid carrier loaded with astilbin (NLC-AS) and tested its effects on the rotenone exposed PC12 cells and in a neurodegenerative mice model of Parkinson’s disease (PD) induced by rotenone. Results show that rotenone caused dose-dependent inhibition of PC12 cell growth with about 50% cell death at 2 µM rotenone. Rotenone caused apoptosis in PC12 cells which was reduced to a notable level by NLC-AS through suppression of oxidative stress, especially via elevation of GSH and total antioxidant capacity, and inhibition of monoamine oxidase. Rotenone significantly augmented neurodegeneration in mouse brains by triggering apoptosis and oxidative damage, while NLC-AS treatment halted these processes. Rotenone-exposed mice showed neuronal deficits and impaired neurocognitive functions like loss of memory and learning restrictions which were restored to a remarkable level by NLC-AS administration. The protective effect of NLC-AS was mediated through the inhibition of GSK3β and induction of Nrf2 genes in the brain tissues. These findings suggest that NLC-AS administration may efficiently regulate the signs of PD in mice and prevent neurodegeneration and neurocognitive dysfunctions.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/3/73_ess23173/figure/73_ess23173.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"8 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Neuroprotective Effect of Sophocarpine against Glutamate-Induced HT22 Cell Cytotoxicity","authors":"Tong Wang, Liying Gao, Jiahua Tan, Dongzhi Zhuoma, Ruiying Yuan, Bin Li, Shan Huang","doi":"10.5650/jos.ess23089","DOIUrl":"https://doi.org/10.5650/jos.ess23089","url":null,"abstract":"</p><p>Neuronal cell death and dysfunction of the central nervous system can be caused by oxidative stress, which is associated with the development of neurodegenerative diseases. Sophocarpine, an alkaloid compound derived from <i>Sophora moorcroftiana</i> (Benth.) Baker seeds, has a wide range of medicinal value. This study sought to determine how sophocarpine exerts neuroprotective effects by inhibited oxidative stress and apoptosis in mouse hippocampus neuronal (HT22) cells. 20mM glutamate-induced HT22 cells were used to develop an in vitro model of oxidative stress damage. The Cell Counting Kit-8 (CCK-8) assay was used to assess cell viability. According to the instructions on the kits to detect reactive oxygen species (ROS) levels and oxidative stress indicators. HT22 cells were examined using immunofluorescence and Western Blotting to detect Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) expression. The expression of proteins and messenger RNA (mRNA) for heme oxygenase-1 (HO-1) was examined by Western Blotting and Quantitative real time polymerase chain reaction (qRT-PCR). Mitochondrial membrane potential (MMP) and Cell apoptosis were used by 5, 5', 6, 6'-Tetrachloro-1, 1', 3, 3'-tetraethyl-imidacarbocyanine iodide (JC- 1) kit and Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick-End Labeling (TUNEL) apoptosis assay kit, respectively. Finally, the expression of pro-apoptotic proteins was detected by Western Blotting. The result demonstrated that sophocarpine (1.25 μM-10 μM) can significantly inhibit glutamate-induced cytotoxicity and ROS generation, improve the activity of antioxidant enzymes. Sophocarpine increased the expression of HO-1 protein and mRNA and the nuclear translocation of Nrf2 to play a cytoprotective role; however, cells were transfected with small interfering RNA targeting HO-1 (si-HO-1) reversed the above effects of sophocarpine. In addition, sophocarpine significantly inhibited glutamate induced mitochondrial depolarization and further inhibited cell apoptosis by reducing the expression level of caspase-related proteins.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/3/73_ess23089/figure/73_ess23089.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"91 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gemini Quaternary Ammonium Surfactants with Different Counterions-modified Montmorillonite for Efficient Removal of Methyl Orange","authors":"Yuanhua Mo, Runyu Cao, Siqi Hu, Bowen Guan, Duojiao Fu, Hongqin Liu, Baocai Xu, Yang Xiao","doi":"10.5650/jos.ess23174","DOIUrl":"https://doi.org/10.5650/jos.ess23174","url":null,"abstract":"</p><p>Organic Na-montmorillonite (OMt-12-2-12·2Y ^– , Y=CH ₃ CO ₃ ^– , C ₆ H ₅ COO ^– and Br ^– ) modified by a series of Gemini quaternary ammonium surfactants with different counterions was prepared for enhancing the adsorption capacity of methyl orange. Compared with the initial adsorption capacity of 5.251 mg/g of Na-Mt, the adsorption effect of OMts under the optimal conditions increased by about 31~34 times. The adsorption isotherms and kinetics of all adsorption processes were respectively described by Langmuir and pseudo-second-order models. The structure, hydrophobicity and hydration of the counterions, as well as the affinity of the counterions with the long aliphatic chains, had a certain influence on the adsorption performance of OMts for methyl orange.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/3/73_ess23174/figure/73_ess23174.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"47 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between Sophorolipids and β-glucan in Aqueous Solutions","authors":"Hiroaki Kaga, Masanori Orita, Koji Endo, Masaaki Akamatsu, Kenichi Sakai, Hideki Sakai","doi":"10.5650/jos.ess23189","DOIUrl":"https://doi.org/10.5650/jos.ess23189","url":null,"abstract":"</p><p>Skin disorders, including acne vulgaris, atopic dermatitis, and rosacea, are characterized by the presence of biofilms, which are communities of microorganisms. The mechanical stability of biofilms is attributed to one of their constituents—polysaccharides—which are secreted by microorganisms. Sophorolipids are biosurfactants with biofilm disruption and removal abilities and are expected to become alternatives for classical petrochemical-based surfactants in cosmetics. <br/>In this study, we investigated the influence of sophorolipids on β-glucan such as dispersion status, interaction mechanism, and configuration change as a model polysaccharide of biofilm in aqueous solution. Dynamic light scattering measurements showed that sophorolipids interfere with the aggregation of β- glucan in aqueous solutions. In contrast, sodium dodecyl sulfate (SDS), which is used as a typical surfactant reference, promotes the aggregation of β-glucan. The interaction between sophorolipids and β-glucan were investigated using surface tension measurements and isothermal titration calorimetry (ITC). Surface tension increased only near critical micelle concentration (CMC) region of sophorolipids in the presence of β-glucan. This suggests that the interaction occurred in the solution rather than at the air–liquid interface. Moreover, the results of ITC indicate that hydrophobic interactions were involved in this interaction. In addition, the results of optical rotation measurements indicate that sophorolipids did not unfold the triple helical structure of β-glucan. β-glucan dispersion was expected to be caused steric hindrance and electrostatic repulsion when sophorolipids interacted with β-glucan via hydrophobic interactions owing to the unique molecular structure of sophorolipids attributed by a bulky sugar moiety and a carboxyl functional group. <br/>These results demonstrated unique performances of sophorolipids on β-glucan and provided more insights on the efficacy of sophorolipids as good anti-biofilms.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/2/73_ess23189/figure/73_ess23189.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"21 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Lipid Composition between Quasipaa spinosa Oil and Rana catesbeiana Oil and Its Effect on Lipid Accumulation in Caenorhabditis elegans","authors":"Lili He, Daren Wu, Jingwen Liu, Guiling Li, Chaoxiang Chen, Emad Karrar, Isam A. Mohamed Ahmed, Lingyu Zhang, Jian Li","doi":"10.5650/jos.ess23143","DOIUrl":"https://doi.org/10.5650/jos.ess23143","url":null,"abstract":"</p><p>Frog oil has been recognized for its nutritional and medicinal value. However, there is limited research on the role of frog oil in preventing obesity. In this study, we aimed to investigate the lipid composition of <i>Quasipaa spinosa</i> oil (QSO) and <i>Rana catesbeiana</i> oil (RCO) using lipidomics analysis. We compared the lipid accumulation effects of these two kinds of frog oils and soybean oil (SO) in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). Additionally, we determined the gene expression related to lipid metabolism and used the <i>nhr-49</i> mutant (RB1716) and <i>sir-2.1</i> mutant (VC199) for validation experiments. The results showed that the lipid composition of QSO and RCO was significantly different (<i>p</i> &lt; 0.05), and QSO was rich in more polyunsaturated fatty acids (PUFAs). After feeding <i>C. elegans</i>, the lipid accumulation of the QSO group was the lowest among the three dietary oil groups. In addition, compared with RCO and SO, QSO significantly inhibited the production of malondialdehyde (MDA) and increased the activity of superoxide dismutase (SOD). The effects of three kinds of dietary oils on the fatty acid composition of <i>C. elegans</i> were significantly different. Compared with SO and RCO, QSO significantly up-regulated (<i>p</i> &lt; 0.05) the expression of <i>sir-2.1</i> and <i>ech-1</i> genes. The results showed that QSO might reduce lipid accumulation through the SIRT1 and nuclear hormone signaling pathways. Such a situation was verified experimentally by the <i>nhr-49</i> mutant (RB1716) and <i>sir-2.1</i> mutant (VC199). This study proposed a new functional oil, laying the groundwork for developing functional foods from <i>Quasipaa spinosa</i>.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/2/73_ess23143/figure/73_ess23143.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ozonated Olive Oil Intake Attenuates Hepatic Steatosis in Obese db/db Mice","authors":"Yasunari Kato, Masami Sakoh, Toshiharu Nagai, Akihiko Yoshida, Hideaki Ishida, Nao Inoue, Teruyoshi Yanagita, Koji Nagao","doi":"10.5650/jos.ess23193","DOIUrl":"https://doi.org/10.5650/jos.ess23193","url":null,"abstract":"</p><p>Chronic inflammation and insulin resistance lead to metabolic syndrome and there is an urgent need to establish effective treatments and prevention methods. Our previous study reported that obese model Zucker (<i>fa/fa</i>) rats fed with ozonated olive oil alleviated fatty liver and liver damage by suppressing inflammatory factors. However, differences among animal species related to the safety and efficacy of ozonated olive oil administration remain unclear. Therefore, this study investigated the effects of oral intake of ozonated olive oil on lipid metabolism in normal mice and mice in the obesity model. C57BL/6J and <i>db/db</i> mice were fed the following AIN-76 diets for four weeks: the mice were either fed a 0.5% olive oil diet (Control diet) or 0.5% ozonated olive oil diet (Oz-Olive diet) in addition to 6.5% corn oil. The results indicated that four weeks of Oz-Olive intake did not adversely affect growth parameters, hepatic lipids or serum parameters in normal C57BL/6J mice. Subsequent treatment of <i>db/db</i> mice with Oz-Olive for four weeks reduced the levels of hepatic triglycerides, serum alkaline phosphatase, and serum insulin. These effects of Oz-Olive administration might be due to suppression of fatty acid synthesis activity and expression of lipogenic genes, as well as suppression of inflammatory gene expression. In conclusion, this study confirmed the safety of Oz-Olive administration in normal mice and its ability to alleviate hepatic steatosis by inhibiting fatty acid synthesis and inflammation in obese mice.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/2/73_ess23193/figure/73_ess23193.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"13 9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}