Lili He, Daren Wu, Jingwen Liu, Guiling Li, Chaoxiang Chen, Emad Karrar, Isam A. Mohamed Ahmed, Lingyu Zhang, Jian Li
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The results showed that the lipid composition of QSO and RCO was significantly different (<i>p</i> < 0.05), and QSO was rich in more polyunsaturated fatty acids (PUFAs). After feeding <i>C. elegans</i>, the lipid accumulation of the QSO group was the lowest among the three dietary oil groups. In addition, compared with RCO and SO, QSO significantly inhibited the production of malondialdehyde (MDA) and increased the activity of superoxide dismutase (SOD). The effects of three kinds of dietary oils on the fatty acid composition of <i>C. elegans</i> were significantly different. Compared with SO and RCO, QSO significantly up-regulated (<i>p</i> < 0.05) the expression of <i>sir-2.1</i> and <i>ech-1</i> genes. The results showed that QSO might reduce lipid accumulation through the SIRT1 and nuclear hormone signaling pathways. Such a situation was verified experimentally by the <i>nhr-49</i> mutant (RB1716) and <i>sir-2.1</i> mutant (VC199). This study proposed a new functional oil, laying the groundwork for developing functional foods from <i>Quasipaa spinosa</i>.</p>\n<p></p>\n<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/2/73_ess23143/figure/73_ess23143.jpg\"/>\ngraphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of Lipid Composition between Quasipaa spinosa Oil and Rana catesbeiana Oil and Its Effect on Lipid Accumulation in Caenorhabditis elegans\",\"authors\":\"Lili He, Daren Wu, Jingwen Liu, Guiling Li, Chaoxiang Chen, Emad Karrar, Isam A. 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Comparison of Lipid Composition between Quasipaa spinosa Oil and Rana catesbeiana Oil and Its Effect on Lipid Accumulation in Caenorhabditis elegans
Frog oil has been recognized for its nutritional and medicinal value. However, there is limited research on the role of frog oil in preventing obesity. In this study, we aimed to investigate the lipid composition of Quasipaa spinosa oil (QSO) and Rana catesbeiana oil (RCO) using lipidomics analysis. We compared the lipid accumulation effects of these two kinds of frog oils and soybean oil (SO) in Caenorhabditis elegans (C. elegans). Additionally, we determined the gene expression related to lipid metabolism and used the nhr-49 mutant (RB1716) and sir-2.1 mutant (VC199) for validation experiments. The results showed that the lipid composition of QSO and RCO was significantly different (p < 0.05), and QSO was rich in more polyunsaturated fatty acids (PUFAs). After feeding C. elegans, the lipid accumulation of the QSO group was the lowest among the three dietary oil groups. In addition, compared with RCO and SO, QSO significantly inhibited the production of malondialdehyde (MDA) and increased the activity of superoxide dismutase (SOD). The effects of three kinds of dietary oils on the fatty acid composition of C. elegans were significantly different. Compared with SO and RCO, QSO significantly up-regulated (p < 0.05) the expression of sir-2.1 and ech-1 genes. The results showed that QSO might reduce lipid accumulation through the SIRT1 and nuclear hormone signaling pathways. Such a situation was verified experimentally by the nhr-49 mutant (RB1716) and sir-2.1 mutant (VC199). This study proposed a new functional oil, laying the groundwork for developing functional foods from Quasipaa spinosa.
期刊介绍：
The J. Oleo Sci. publishes original researches of high quality on chemistry, biochemistry and science of fats and oils
such as related food products, detergents, natural products,
petroleum products, lipids and related proteins and sugars.
The Journal also encourages papers on chemistry and/or biochemistry as a major component combined with biological/
sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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