{"title":"Activation of gut microbiota-hif1α axis effectively restores resistance to Aeromonas veronii caused by improper administration of AiiO-AIO6.","authors":"Hongwei Yang, Rui Xia, Tsegay Teame, Delong Meng, Shenghui Li, Tiantian Wang, Qianwen Ding, Yuanyuan Yao, Xiaoqing Xu, Yalin Yang, Chao Ran, Yaqing Zhang, Shengkang Li, Benjamin Niemann, Le Luo Guan, Zhen Zhang, Zhigang Zhou","doi":"10.1016/j.tjnut.2025.03.007","DOIUrl":"https://doi.org/10.1016/j.tjnut.2025.03.007","url":null,"abstract":"<p><strong>Background: </strong>Feeding adult zebrafish a diet supplemented with quenching enzyme AiiO-AIO6 for 3 wk improved the growth performance and disease resistance. However, when the feeding period was extended to 8 wk, zebrafish's disease resistance to Aeromonas veronii decreased.</p><p><strong>Objective: </strong>We investigated the mechanisms of the reduced disease resistance of zebrafish induced by feeding on AiiO-AIO6 supplemented diet for a long-term (8 wk), and assessed the effectiveness of feed additives in restoring the low disease resistance.</p><p><strong>Methods: </strong>One-month-old (adult) zebrafish were fed with a basal diet and the basal diet supplemented with AiiO-AIO6 (10 U/g) for 8 wk (Expt. 1). Furthermore, zebrafish larvae model (Expt. 2) was developed and used to study the mechanisms how AiiO-AIO6 affected the disease resistance (Expt. 3). We also investigated the effectiveness of selected prebiotic tributyrin, β-glucan or mannan in activating gut microbiota-hif1α to restore the low disease resistance of adult zebrafish fed with AiiO-AIO6 for 8 wk (Expt. 4). Lastly, the effects of Bacillus subtilis in activating the gut microbiota-hif1α and improving the low disease resistance of zebrafish larvae induced by AiiO-AIO6 was examined (Expt. 5).</p><p><strong>Results: </strong>Feeding adult zebrafish with AiiO-AIO6 for 8 wk promoted the growth but disordered the gut microbiota and reduced disease resistance. Zebrafish larvae model confirmed that feeding AiiO-AIO6 for 2 d increased disease resistance, while 7 d decreased the resistance by suppressing hif1α. Using a germ-free zebrafish larvae model, we also demonstrated that AiiO-AIO6-induced gut microbiota mediated inhibition of hif1α. Furthermore, zebrafish fed on the AiiO-AIO6 containing diet supplement with tributyrin, β-glucan, mannan, or B. subtilis activated the gut microbiota-hif1α axis to reverse the low resistance caused by AiiO-AIO6.</p><p><strong>Conclusion: </strong>Activating the gut microbiota-hif1α axis has vital role in improving intestinal health and restores the low resistance to A. veronii caused by improper administration of dietary AiiO-AIO6 in zebrafish.</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Extracellular vesicles from Lactobacillus fermentum enhance intestinal barrier integrity and restore gut microbial homeostasis in experimental murine colitis.","authors":"Yanwei Wang, Tiantian Li, Zhuo Dong, Qiyue Zhang, Jingqiu Mi, Qingfeng Wang, Gang Lin, Qiugang Ma, Ru Jia, Shimeng Huang","doi":"10.1016/j.tjnut.2025.03.001","DOIUrl":"https://doi.org/10.1016/j.tjnut.2025.03.001","url":null,"abstract":"<p><strong>Background: </strong>Lactobacillus fermentum (L. fermentum) has been shown to improve intestinal health and treat colitis; however, its precise efficacy and mechanisms in inflammatory bowel disease (IBD) remain unclear.</p><p><strong>Objectives: </strong>This study aimed to evaluate whether L. fermentum and its metabolites, extracellular vesicles, and other components could modulate intestinal barrier function and gut microbiota to alleviate dextran sulfate sodium (DSS)-induced colitis in mice.</p><p><strong>Methods: </strong>Forty-eight mice were randomly assigned to six groups: Control (CON), DSS, L. fermentum + DSS group (LF + DSS), heat-inactivated L. fermentum + DSS group (LHF + DSS), L. fermentum supernatant solution + DSS group (LSF + DSS), and L. fermentum extracellular vesicles + DSS group (LEV + DSS). After a one-week acclimation, mice were gavaged daily for three weeks. Fresh cultures, including live (LF + DSS), heat-inactivated (LHF + DSS), supernatant (LSF + DSS), and extracellular vesicles (LEV + DSS), were prepared daily. During the final seven days, the control group received normal water, while the other groups received 3% DSS. Data were collected daily, followed by sample collection from the mice.</p><p><strong>Results: </strong>Herein, significant reductions (P < 0.05) in body weight changes, disease activity index (DAI), intestinal damage, and histology scores were observed in the treatment groups, especially LEV + DSS and LF + DSS. Additionally, compared with the DSS group, colonic mucus secretion, as well as claudin-1 and occludin expression, increased significantly (P < 0.05) in the LEV + DSS and LF + DSS groups, while proinflammatory cytokines interleukin (IL) -1β and tumor necrosis factor-α (TNF-α) decreased (P < 0.05) and IL-10 increased (P < 0.05) in the LEV + DSS group. L. fermentum and its components significantly regulated gut microbiota α-diversity and β-diversity, affecting overall composition. LEfSe analysis revealed an enrichment of beneficial bacteria including Prevotellaceae_UCG-001, Romboutsia, and Ruminococcus in the LF + DSS group, and Akkermansia, Odoribacter, and Marvinbryantia in the LEV + DSS group. Both L. fermentum and its extracellular vesicles significantly downregulated the gene expression of TNF-α and IL-1β, while upregulating the expression of IL-10, thereby contributing to the alleviation of colitis symptoms.</p><p><strong>Conclusions: </strong>This study reveals that L. fermentum alleviates colitis through modulation of the gut microbiota and reinforcement of the intestinal mucosal barrier, with its extracellular vesicles potentially playing a key role in this regulatory process.</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Association Between Human Milk Oligosaccharides and Early Adiposity Rebound in Children: A Case-Control Study of the Tohoku Medical Megabank Project Birth and Three-Generation Cohort Study.","authors":"Kento Sawane, Ippei Takahashi, Mami Ishikuro, Hiroko Takumi, Masatsugu Orui, Aoi Noda, Genki Shinoda, Hisashi Ohseto, Tomomi Onuma, Fumihiko Ueno, Keiko Murakami, Naoko Higuchi, Tomoko Tanaka, Takashi Furuyashiki, Tomohiro Nakamura, Seizo Koshiba, Kinuko Ohneda, Kazuki Kumada, Soichi Ogishima, Atsushi Hozawa, Junichi Sugawara, Shinichi Kuriyama, Taku Obara","doi":"10.1016/j.tjnut.2025.02.024","DOIUrl":"https://doi.org/10.1016/j.tjnut.2025.02.024","url":null,"abstract":"<p><strong>Background: </strong>Adiposity rebound (AR) is the point when the body mass index (BMI) begins to rise again during early childhood. Early AR (before age 5) is associated with a higher risk of lifelong obesity and metabolic disorders and may be influenced by breastfeeding. Although human milk oligosaccharides (HMOs) in breast milk are crucial for child growth, their association with AR status has not been studied.</p><p><strong>Objective: </strong>To explore breast milk HMO compositions and molecules associated with AR status in children.</p><p><strong>Methods: </strong>In this case-control study, we included 184 mother-child pairs from the Tohoku Medical Megabank Project Birth and Three-Generation (TMM BirThree) Cohort Study (93 AR cases, 91 controls). Breast milk was collected 1 month postpartum, and the concentration of 15 HMO molecules and alpha-diversity index (Inverse Simpson index) were quantified. Wilcoxon's rank-sum test and partial least squares-discriminant analysis (PLS-DA) identified candidate HMOs, and multivariable logistic regression analysis evaluated associations between candidate HMOs and AR status. Analyses were stratified by maternal secretor status (secretor or non-secretor).</p><p><strong>Results: </strong>In secretor mothers, multivariable logistic regression showed that the Inverse Simpson index (OR, 0.54 [95% CI, 0.36-0.82]), sum of sialic acid-bound HMOs (0.61 [0.41-0.91]), and 3'-sialyllactose (0.67 [0.46-0.98]) were inversely associated with early AR in a fully adjusted model. A trend of interaction between sialyl-lacto-N-tetraose a (LSTa) and maternal secretor status on AR was observed in a fully adjusted model (P-value for interaction = 0.051).</p><p><strong>Conclusions: </strong>Alpha diversity, sialic acid-bound HMOs, and 3'SL may be involved in inhibiting AR in children of secretor mothers, and a trend of interactive effect of LSTa among maternal secretor status on AR was indicated. These findings offer novel perspectives on the associations between breastfeeding and a child's adiposity as well as potential metabolic disorders later in life. REGISTRY NUMBER/WEBSITE: https://www.umin.ac.jp/ (trial registration number: UMIN000047160).</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dietary gluten intake and cardiovascular disease mortality in Japanese adults in the Takayama study.","authors":"Michiko Tsuji, Keiko Wada, Michiyo Yamakawa, Masaaki Sugino, Tomoka Mori, Chisato Nagata","doi":"10.1016/j.tjnut.2025.03.005","DOIUrl":"https://doi.org/10.1016/j.tjnut.2025.03.005","url":null,"abstract":"<p><strong>Background: </strong>There is growing attention toward the gluten-free diet in Japan, in spite of a low prevalence of celiac disease and high consumption of rice.</p><p><strong>Objectives: </strong>The present study examined whether gluten intake is associated with an increased risk of cardiovascular disease (CVD) mortality in Japanese adults.</p><p><strong>Methods: </strong>In 1992, 13,355 men and 15,724 women, 35 years of age and older, in the Takayama study completed a self-administered questionnaire. Gluten intake was estimated using a food-frequency questionnaire as the baseline. Mortality was ascertained during 16 years of follow-up. Hazard ratios (HRs) and 95% CIs for CVD mortality according to gluten intake quartiles were calculated.</p><p><strong>Results: </strong>During 16.1 years of follow-up, 775 CVD deaths in men and 903 CVD deaths in women occurred. As compared with the lowest quartile of intake, the highest quartile of gluten intake was significantly associated with a decreased risk of CVD mortality after controlling for age, sex and other covariates (HR = 0.73; 95% CI: 0.62-0.86, P-trend = 0.0003).</p><p><strong>Conclusions: </strong>Our data showed a significant inverse association between gluten intake and CVD mortality. The present study does not support the notion that gluten avoidance should have a beneficial effect on CVD mortality.</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christopher D Nulty, Janette Walton, Robert M Erskine
{"title":"Habitual dietary collagen intake is lower in women and older Irish adults compared to younger men.","authors":"Christopher D Nulty, Janette Walton, Robert M Erskine","doi":"10.1016/j.tjnut.2025.03.002","DOIUrl":"https://doi.org/10.1016/j.tjnut.2025.03.002","url":null,"abstract":"<p><strong>Background: </strong>Collagen ingestion is purported to benefit connective tissues, such as skin, bone, muscle, tendon, and ligament. However, the quantity of collagen intake in the diet of European adults is unknown.</p><p><strong>Objective: </strong>To investigate collagen intake in the habitual diets of Irish adults, and whether it differed according to sex and/or age.</p><p><strong>Methods: </strong>We conducted secondary analysis of the Irish National Adult Nutrition Survey, which assessed typical dietary intake using a four-day food diary in 1,500 adults, aged 18-90 years. We categorized participants into three age groups: young (18-39 years, n=630), middle-aged (40-64 years, n=644), and older (≥65 years, n=226) adults. Collagen composition of each individual food item in the database was determined by applying a percentage collagen value from analytical sources, allowing computation of collagen mean daily intake (MDI), collagen MDI relative to body mass, and collagen/total protein MDI. Differences in intakes between age groups and sexes were evaluated using physical activity level as a covariate.</p><p><strong>Results: </strong>Collagen MDI for the entire population was 3.2±2.0 g∙day<sup>-1</sup>, representing 3.6±1.9% total protein intake. Men had higher absolute and relative collagen MDI than women, regardless of age (4.0±2.1 g∙day<sup>-1</sup> vs. 2.3±1.4 g∙day<sup>-1</sup>, p<0.001), while older adults had lower absolute collagen MDI than middle-aged adults (2.9±1.8 g∙day<sup>-1</sup> vs. 3.3±2.0g∙day<sup>-1</sup>, p=0.021).</p><p><strong>Conclusions: </strong>Collagen intake in the Irish adult population was considered low (relative to total protein intake and to dose-response studies), particularly in women and older individuals. Increasing daily collagen intake may therefore be warranted to optimise the health of collagen-rich tissues.</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of the Effectiveness of Protein Supplementation Combined with Resistance Training on Body Composition and Physical Function in Healthy Elderly Adults","authors":"Haiping Tian , Wanwan Qiao , Xianxiu Wen","doi":"10.1016/j.tjnut.2025.01.017","DOIUrl":"10.1016/j.tjnut.2025.01.017","url":null,"abstract":"<div><h3>Background</h3><div>The global population of individuals over 65 y is expected to reach 426 million by 2050. Aging is associated with a progressive loss of muscle mass, strength, and function, leading to sarcopenia and adverse outcomes such as physical disability and increased mortality. Interventions such as resistance training and protein supplementation have shown promise in mitigating these effects.</div></div><div><h3>Objectives</h3><div>To determine the comparative effectiveness of protein supplementation, resistance training, and their combination on body composition and physical function in healthy older adults through a network meta-analysis.</div></div><div><h3>Methods</h3><div>We conducted a systematic review and network meta-analysis following PRISMA guidelines and registered it in PROSPERO (CRD42021226561). We included randomized controlled trials comparing protein supplementation, resistance training, and their combination in participants aged ≥50 y. Data were extracted from PubMed, Web of Science, Embase, and Cochrane Library. The risk of bias was assessed using the Cochrane Collaboration Risk of Bias Tool.</div></div><div><h3>Results</h3><div>A total of 38 randomized controlled trials involving 2610 participants were included. The combined intervention of protein supplementation and resistance training significantly improved lean body mass (standardized mean difference [SMD]: 0.44; 95% confidence interval [CI]: 0.05, 0.95) compared with protein supplementation alone. The combined intervention also showed significant improvements in muscle mass (SMD: 1.49; 95% CI: 0.11, 2.67). The combined intervention (SMD: 2.74; 95% CI: 0.76, 4.74) and resistance training alone (SMD: 2.53, 95% CI: 0.29, 4.84) significantly improved muscle strength compared with controls. The combined intervention (SMD: 4.98; 95% CI: 2.72, 7.17) and resistance training alone (SMD: 4.52; 95% CI: 2.30, 6.64) significantly improved physical function compared with protein supplementation alone.</div></div><div><h3>Conclusions</h3><div>Combining exercise and protein supplementation is the most effective method for improving muscle mass, strength, and physical function in older adults. This approach should be considered to enhance physical health in this population. Future large-scale trials are necessary to confirm these findings.</div></div>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":"155 3","pages":"Pages 764-774"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eliana Lucchinetti , Phing-How Lou , Akash Chakravarty , Camila Schultz Marcolla , Mirielle L Pauline , Pamela R Wizzard , Catherine J Field , Eytan Wine , Martin Hersberger , Paul W Wales , Justine M Turner , Stefanie D Krämer , Michael Zaugg
{"title":"The Novel Lipid Emulsion Vegaven Is Well Tolerated and Elicits Distinct Biological Actions Compared With a Mixed-Oil Lipid Emulsion Containing Fish Oil: A Parenteral Nutrition Trial in Piglets","authors":"Eliana Lucchinetti , Phing-How Lou , Akash Chakravarty , Camila Schultz Marcolla , Mirielle L Pauline , Pamela R Wizzard , Catherine J Field , Eytan Wine , Martin Hersberger , Paul W Wales , Justine M Turner , Stefanie D Krämer , Michael Zaugg","doi":"10.1016/j.tjnut.2024.10.047","DOIUrl":"10.1016/j.tjnut.2024.10.047","url":null,"abstract":"<div><h3>Background</h3><div>Vegaven is a novel lipid emulsion for parenteral nutrition (PN) based on 18-carbon n–3 (ω-3) fatty acids, which elicits liver protection via interleukin-10 (IL-10) in the murine model of PN.</div></div><div><h3>Objectives</h3><div>In a preclinical model of PN in neonatal piglets, Vegaven was tested for efficacy and safety and compared with a mixed-oil lipid emulsion containing fish oil (SMOFlipid).</div></div><div><h3>Methods</h3><div>Male piglets 4–5 d old were randomly allocated to isocaloric isonitrogenous PN for 14 d, which varied only by the type of lipid emulsion (Vegaven, <em>n</em> = 8; SMOFlipid, <em>n</em> = 8). Hepatic IL-10 tissue concentration served as primary outcome. Secondary outcomes were organ weights, bile flow, blood analyses, plasma insulin and glucagon concentrations, insulin signaling, proinflammatory cytokines, tissue lipopolysaccharide concentrations, and fatty acid composition of phospholipid fractions in plasma, liver, and brain.</div></div><div><h3>Results</h3><div>Total weight gain on trial, organ weights, and bile flow were similar between the Vegaven and the SMOFlipid group. Vegaven elicited higher hepatic IL10 (Δ = 148 pg/mg protein; <em>P</em> < 0.001) and insulin receptor substrate-2 amounts (Δ = 0.08 OD; <em>P</em> = 0.012). Plasma insulin concentrations (Δ = 1.46 mU/L; <em>P</em> = 0.003) and fructosamine (glycated albumin, Δ = 12.4 μmol/g protein; <em>P</em> = 0.003) were increased in SMOFlipid as compared with those of Vegaven group, indicating insulin resistance. Higher hepatic injury markers were observed more frequently in the SMOFlipid group than those in the Vegaven group. Lipopolysaccharide, tumor necrosis factor-α, and IL-6 concentrations increased in pancreatic and brain tissues of SMOFlipid-treated compared with those in the Vegaven-treated piglets. Insulin signaling reduced in the brains of SMOFlipid-treated piglets. Vegaven and SMOFlipid elicited distinct fatty acid profiles in the phospholipid fractions of the rapidly growing brains but showed similar accretion of docosahexaenoic acid and arachidonic acid after 2 wk of PN.</div></div><div><h3>Conclusions</h3><div>Vegaven is well tolerated in this piglet model of PN, demonstrating distinct biological actions compared with SMOFlipid, namely lower liver, pancreas, and brain inflammation, enhanced insulin signaling, and improved whole body glucose control.</div></div>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":"155 3","pages":"Pages 703-718"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sara Henkel , Petra Frings-Meuthen , Christina Diekmann , Martin Coenen , Birgit Stoffel-Wagner , Robert Németh , Dominik Pesta , Sarah Egert
{"title":"Influence of Ambient Temperature on Resting Energy Expenditure in Metabolically Healthy Males and Females","authors":"Sara Henkel , Petra Frings-Meuthen , Christina Diekmann , Martin Coenen , Birgit Stoffel-Wagner , Robert Németh , Dominik Pesta , Sarah Egert","doi":"10.1016/j.tjnut.2025.01.013","DOIUrl":"10.1016/j.tjnut.2025.01.013","url":null,"abstract":"<div><h3>Background</h3><div>It is not yet clear to what extent the physiologic regulatory mechanisms that maintain core body temperature are reflected by changes in resting energy expenditure (REE). Particularly, in indirect calorimetry with a canopy, the effects of short-term temperature exposures have not yet been investigated. This can be of relevance for the determination of REE in practice.</div></div><div><h3>Objectives</h3><div>This randomized crossover study investigated the influence of a 30-min exposure to 18 °C (cool room temperature), 22 °C (room temperature), 28 °C (thermoneutral zone), and 38 °C (heat) on REE determined by assessing the inhaled oxygen volume and the exhaled carbon dioxide volume via indirect calorimetry on 4 consecutive days.</div></div><div><h3>Methods</h3><div>In total, 32 metabolically healthy participants [16 males and 16 females, age: 25 ± 3 y, body mass index (BMI): 22.4 ± 1.6 kg/m<sup>2</sup>] were included in the study after screening examination.</div></div><div><h3>Results</h3><div>Lean body mass, ambient temperature, and heart rate were the most important determinants (all <em>P</em> < 0.001) and explained 61.3% of the variance in REE. A multivariate linear mixed model analysis revealed that lean mass (15.87 ± 3.66; <em>P</em> < 0.001) and ambient temperature (<em>P</em> = 0.001) significantly influenced REE. REE significantly differed between 18 and 28 °C (18 °C: +96 ± 24 kcal/24 h; <em>P</em> < 0.001), 22 and 28 °C (22 °C: +73 ± 24 kcal/24 h; <em>P</em> = 0.003), and 18 and 38 °C (18 °C: +57 ± 23 kcal/24 h; <em>P</em> = 0.016).</div></div><div><h3>Conclusions</h3><div>Effects of ambient temperature on REE, especially cold, are detectable after only brief exposure, emphasizing the importance of performing indirect calorimetry with a canopy under controlled environmental conditions.</div><div>The study was registered at <span><span>clinicaltrials.gov</span><svg><path></path></svg></span> as NCT05505240 (Influence of Ambient Temperature on Resting Energy Expenditure of Healthy Adults - Full Text View - <span><span>ClinicalTrials.gov</span><svg><path></path></svg></span>).</div></div>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":"155 3","pages":"Pages 862-870"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advances of Selenium in Poultry Nutrition and Health","authors":"Zi-Jian Xu , Fa-Zheng Ren , Zi-Wei Zhang , Shi-Wen Xu , Jia-Qiang Huang","doi":"10.1016/j.tjnut.2025.01.014","DOIUrl":"10.1016/j.tjnut.2025.01.014","url":null,"abstract":"<div><div>Selenium is widely acknowledged as an indispensable trace element for humans and various animals, including poultry. The addition of selenium in appropriate doses plays a crucial role in promoting poultry growth and reproduction. Conversely, both deficiency and excessive intake of selenium can pose significant threats to poultry health and production performance. In modern poultry farming, there is an increasing demand for precise nutrient intake, necessitating a comprehensive understanding of the multifaceted role of selenium. This review aimed to compare and contrast the properties and recommended addition amounts of different sources of selenium in poultry feed; to discuss the hazards and mechanisms associated with selenium deficiency or excess in poultry; to summarize the pivotal role that selenium plays in stress states among poultry. Overall, this review seeked to provide a comprehensive overview highlighting the significance of selenium in terms of nutrition and health for poultry while ensuring optimal utilization within poultry production.</div></div>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":"155 3","pages":"Pages 676-689"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}