Journal of Obstetrics and Gynaecology Research最新文献

{"title":"Vaginal metabolic profiling reveals biomarkers characteristics of high-risk HPV infection and cervical lesions","authors":"Xiaoling Huang,&nbsp;Xiaoge Li,&nbsp;Sijing Li,&nbsp;Jin Wu,&nbsp;Zhaoning Duan,&nbsp;Ming Luo,&nbsp;Ying Jia","doi":"10.1111/jog.70035","DOIUrl":"10.1111/jog.70035","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to identify potential biomarkers in high-risk human papillomavirus (HR-HPV) infections and related cervical lesions through the analysis of vaginal metabolic profiles.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Patients and methods</h3>\u0000 \u0000 <p>Forty cervicovaginal secretion samples were collected from healthy individuals, HR-HPV-positive individuals, low-grade squamous intraepithelial lesion patients, high-grade squamous intraepithelial lesion patients, and cervical cancer (CC) patients. All samples were analyzed using liquid chromatography–mass spectrometry (LC–MS). Metabolites with significant differences were identified using the variable importance in projection (VIP &gt; 1) derived from the orthogonal least partial squares discriminant analysis (OPLS-DA) model and the <i>p</i>-value (<i>p</i> &lt; 0.05) of the Student's <i>t</i> test. Utilizing hierarchical cluster analysis and receiver operating characteristic curve analysis, the optimal biomarkers were identified, and the diagnostic potential of these biomarkers was evaluated. Significantly enriched pathways of the differential metabolites were explored using metabolic pathway analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>LC–MS analysis of the vaginal metabolomic profiles revealed unique molecular characteristics of different states of HR-HPV infection. Based on persistent HR-HPV infection, 3-Isochromanone increased, and DG(2:0/5-iso PGF2VI/0:0) and (4R,5S,7R,11x)-11,12-Dihydroxy-1(10)-spirovetiven-2-one 12-glucoside decreased (<i>p</i> &lt; 0.05) with the aggravation of cervical lesions, suggesting their potential as biomarkers to predict various stages of cervical lesions. Additionally, analysis of metabolic pathway enrichment for differential metabolites identified the phenylalanine, tyrosine, and tryptophan biosynthesis pathways as the key metabolic pathways in the progression of cervical lesions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>By measuring the metabolome of vaginal secretions, specific metabolites can effectively distinguish persistent HR-HPV infections and different grades of cervical lesions, which may become an important means for the early screening and diagnosis of CC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic etiologies associated with non-immune hydrops fetalis delineated by whole exome sequencing: A pilot series and its implications in prenatal genetic counseling","authors":"Zhu Wei Lim,&nbsp;Gwo-Chin Ma,&nbsp;Ting-Yu Chang,&nbsp;Wan-Ju Wu,&nbsp;Mei-Hui Lee,&nbsp;Ming Chen","doi":"10.1111/jog.70039","DOIUrl":"10.1111/jog.70039","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Non-immune hydrops fetalis (NIHF) is a spectrum of disease involving heterogeneous etiologies. Prenatal application of whole exome sequencing (WES) has emerged as a valuable tool to decipher genetic causes in structural abnormalities detected by fetal ultrasound. Here, we reviewed index cases of NIHF whose genetic etiologies were delineated by WES.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We conducted a retrospective study on our pilot series (<i>n</i> = 12) of NIHF that underwent WES with outcomes systematically reviewed. For each case, we performed a detailed sonographic examination for anasarca, virology profile, karyotyping, and chromosomal microarray analysis (CMA), in addition to WES performed either prenatally or postnatally.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Between December 2019 and July 2024, 12 fetuses with abnormal fluid accumulation in fetal compartments, despite normal karyotyping and CMA, underwent WES to elucidate potential genetic etiologies. Variant interpretation was performed in accordance with the guidelines of the American College of Medical Genetics and Genomics (ACMG). A definitive molecular diagnosis was established in all 12 cases, with identified disorders including lymphatic abnormalities (33%), musculoskeletal disorders (30%), and syndromic conditions (17%). All recurrent cases (3/3, 100%) and those who received fetal therapy (4/4, 100%) have a definite molecular diagnosis. Two survived fetuses were diagnosed in the first and third trimesters, respectively, while four infants died and six couples decided to terminate the pregnancy due to disease progression. Two novel founder variants (<i>HSPG2</i> and <i>BBS2</i>) were found.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>WES is an effective tool to determine the genetic diagnosis of NIHF cases and would discover more novel causative genes to match the prenatal phenotypes exhibited.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144814999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balancing ethics and clinical need: The Japanese experience with preimplantation genetic testing for monogenic disease regulation and implementation","authors":"Kou Sueoka","doi":"10.1111/jog.70033","DOIUrl":"10.1111/jog.70033","url":null,"abstract":"<p>Preimplantation genetic testing (PGT) has emerged as an important extension of assisted reproductive technologies. Alongside these technological advances, PGT has also generated substantial ethical and social debate, particularly regarding the sanctity of human life. Ethical perspectives are deeply context-dependent and vary significantly across different backgrounds. The unique historical and social context of Japan necessitated careful ethical discussions as PGT was introduced. The Japan Society of Obstetrics and Gynecology (JSOG) provided official guidance under which PGT was implemented. With these technological advancements, reliable and highly accurate genetic analysis has become possible across a broad spectrum of pathogenic genotypes up to this stage. However, the most challenging issues have been the ethical debate surrounding indication criteria that prioritize severity. “Severity” is a highly nuanced and multifaceted concept, making the establishment of a clear, universally applicable standard inherently difficult. JSOG's first ethical approval for PGT in Duchenne muscular dystrophy cases led to the birth of the first child conceived via PGT at Keio University Hospital in 2004 in Japan, followed by over 13 years of research and ongoing discussion that addressed issues such as disease definition, severity criteria, scope of genetic analysis, disclosure policies, and safety and accuracy concerns. However, for certain genetic disorders, determining the appropriateness of PGT solely based on genotype or the clinical course observed in affected family members can be challenging, such as triplet repeat diseases or mitochondrial diseases. Ethical debate must be seen not as an obstacle to progress, but as an ongoing process of reflective engagement, offering context-dependent guidelines rather than absolute prohibitions. Within this delicate balance of complex debate, constructive engagement between ethics and scientific progress carries a profound responsibility to serve as a driving force in advancing the health and well-being of humanity.</p>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://obgyn.onlinelibrary.wiley.com/doi/epdf/10.1111/jog.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144814998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of shear wave elastography in predicting clinical symptoms in adenomyosis: A prospective observational study with a machine learning approach","authors":"Uğurcan Zorlu,&nbsp;Sezer Nil Yılmazer Zorlu,&nbsp;Burak Elmas","doi":"10.1111/jog.70037","DOIUrl":"10.1111/jog.70037","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Adenomyosis is a gynecological condition characterized by the invasion of endometrial tissue into the myometrium, causing symptoms such as dysmenorrhea, menorrhagia, and chronic pelvic pain. Its diagnosis remains challenging due to overlapping features with other uterine disorders, and the variability in symptom presentation makes management complex. This study aims to evaluate the utility of shear wave elastography (SWE) in predicting clinical symptoms of adenomyosis and to explore the potential of machine learning (ML) models in enhancing diagnostic precision and predicting patient outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 63 women diagnosed with focal adenomyosis were included in this prospective observational study. SWE was performed to assess uterine tissue stiffness, with ML algorithms (logistic regression, random forest, support vector machine, K-nearest neighbors) applied to predict symptoms based on SWE measurements and clinical features. Clinical symptoms such as dysmenorrhea, dyspareunia, non-cyclic chronic pelvic pain, and menorrhagia were evaluated. Statistical analysis was conducted using SPSS software, with performance metrics such as accuracy, F1 score, and ROC-AUC used to assess model effectiveness.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant associations were found between SWE velocity (SWV) values and symptoms like dysmenorrhea, dyspareunia, and non-cyclic chronic pelvic pain. K-nearest neighbors (KNN) exhibited the best performance in predicting dyspareunia and non-cyclic chronic pelvic pain, while random forest performed best for dysmenorrhea. Menorrhagia did not show significant differences in SWE values. Cutoff values for clinical symptoms, such as 4.69 m/s for dysmenorrhea, were identified, providing actionable thresholds for clinical use.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>SWE combined with ML offers a promising approach to predict clinical symptoms of adenomyosis, aiding in personalized treatment strategies. This study highlights the potential of integrating advanced imaging techniques and computational models to enhance clinical decision-making and improve patient outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory analysis of contributory factors for cerebral palsy during labor involving neuraxial analgesia: From the Japan Obstetric Compensation System for Cerebral Palsy","authors":"Tatsuya Arakaki,&nbsp;Junichi Hasegawa,&nbsp;Akihiko Sekizawa,&nbsp;Tomoaki Ikeda,&nbsp;Isamu Ishiwata","doi":"10.1111/jog.70030","DOIUrl":"10.1111/jog.70030","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This study aimed to reveal factors contributing to cerebral palsy owing to intrapartum hypoxia during labor involving neuraxial analgesia using a nationwide database in Japan.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We retrospectively reviewed 102 reports of cases involving the use of anesthesia during labor, selected from reports on the causes of cerebral palsy, which were analyzed by the Japan Obstetric Compensation System Causal Analysis Committee between 2009 and 2019. Among these, 85 deliveries involved neuraxial analgesia. Of these, 39 cases were identified in which cerebral palsy was associated with intrapartum hypoxia and acidosis. These cases were classified into three categories based on the presumed contributing factors to cerebral palsy, namely anesthesia-related complications, obstetric complications, and labor progress. Clinical characteristics and intrapartum management issues were descriptively analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Neuraxial analgesia was implicated in five of the 39 cases (12.8%), with direct involvement in one case of total spinal anesthesia. Obstetric complications (20.5%) and labor progress-related issues (76.9%) were more frequently associated with cerebral palsy. Intrapartum management problems were identified in most cases, including inappropriate cardiotocography interpretation (79.5%) and uterotonic use (38.5%). Anesthesia-related concerns, such as insufficient maternal or fetal monitoring, were observed in some cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Neuraxial analgesia was infrequently the direct cause of cerebral palsy; however, suboptimal labor management was identified in many cases. Improved monitoring, timely intervention, and standardized protocols are essential to reduce cerebral palsy risk during neuraxial analgesia-supported deliveries. Although rare, serious anesthesia-related complications also underscore the significance of vigilance in anesthetic practice.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and survival outcome of vulvar cancer in South Korea: A nationwide study from the Cancer Public Library Database","authors":"Chel Hun Choi,&nbsp;Ha kyun Chang,&nbsp;Gi Hwan Bae,&nbsp;Jong Ha Hwang","doi":"10.1111/jog.70029","DOIUrl":"10.1111/jog.70029","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Vulvar cancer, although rare, presents clinical challenges due to diverse histological subtypes and varying prognoses. This study aimed to examine the clinical characteristics and survival outcomes of vulvar cancer in South Korea.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A retrospective cohort study was conducted using data from the Cancer Public Library Database, which includes information from the Korea National Cancer Incidence Database, Statistics Korea, and National Health Insurance databases. The study analyzed data from South Korean women diagnosed with vulvar cancer between 2014 and 2016.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Out of 1242 initial patients, 292 met the inclusion criteria. The untreated group (<i>n</i> = 62) had a higher proportion of patients aged 80 and above (35.5%) and a higher mean Charlson Comorbidity Index (CCI) score compared to the treatment group (<i>n</i> = 230). The treatment group showed significantly higher survival probability (adjusted HR 0.358, 95% CI: 0.24–0.54) compared to the untreated group. Surgical intervention alone reduced mortality risk significantly (adjusted HR 0.20, 95% CI: 0.12–0.33), particularly among those diagnosed at an earlier clinical stage. Histologically, basal cell carcinoma had the highest survival probability with a significantly lower mortality risk than squamous cell carcinoma (adjusted HR 0.11, 95% CI: 0.02–0.82). Adenocarcinoma also showed improved survival outcomes (adjusted HR 0.42, 95% CI: 0.19–0.94).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study emphasizes the importance of early diagnosis and surgical treatment in improving survival outcomes for vulvar cancer patients. It also highlights the need to consider demographic and clinical factors in treatment planning and calls for region-specific studies to address unique epidemiological patterns.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concerns regarding methodology and statistical validity in the manuscript on medical support for victims of sexual violence","authors":"Mücahit Furkan Balci,&nbsp;İbrahim Karaca","doi":"10.1111/jog.70024","DOIUrl":"10.1111/jog.70024","url":null,"abstract":"","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative immuno-profiling of proliferative and disordered proliferative endometrium in anovulatory abnormal uterine bleeding","authors":"Mishu Mangla,&nbsp;Seetu Palo,&nbsp;Poojitha Kalyani Kanikaram,&nbsp;P. Raja Shekar,&nbsp;Aparna Setty","doi":"10.1111/jog.70032","DOIUrl":"10.1111/jog.70032","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Anovulatory abnormal uterine bleeding (AUB) is frequently associated with prolonged unopposed estrogen exposure, resulting in histological patterns ranging from proliferative to disordered proliferative endometrium (DPE). While these entities are histologically distinguishable, their immunological microenvironments remain underexplored.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To compare the immune cell profile of proliferative and DPE in women with anovulatory AUB, focusing on key immune markers: CD4, CD8, CD56, and CD68.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>wEndometrial biopsies from women diagnosed with anovulatory AUB were classified histologically as proliferative or disordered proliferative. Immunohistochemical staining was performed to quantify CD4+ helper T cells, CD8+ cytotoxic T cells, CD56+ natural killer cells, and CD64+ macrophages. Quantitative analysis was conducted using high-power field (hpf) counts, and statistical comparisons were made using appropriate non-parametric tests.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 73 women with AUB were analyzed, including 30 with proliferative and 43 with DPE. No statistically significant differences were observed between the two groups in age, body mass index (BMI), endometrial thickness, Pictorial Blood Assessment Chart score, or immune cell counts (CD4, CD8, CD68, CD56/hpf). Correlation analysis revealed significant positive associations among immune markers, particularly between CD4 and CD8 (r = 0.425, <i>p</i> &lt; 0.001), CD8 and CD68 (r = 0.316, <i>p</i> = 0.006), and CD4 and CD56 (r = 0.377, <i>p</i> = 0.001). CD56+ cells also showed a negative correlation with BMI (r = −0.251, <i>p</i> = 0.032) and a positive correlation with duration of bleeding (r = 0.289, <i>p</i> = 0.013).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The local immune cell infiltration may not differ substantially between proliferative and DPE in the context of AUB. No statistically significant differences were observed between the two groups with respect to clinical parameters, endometrial thickness, or immune cell marker expression (CD4, CD8, CD68, and CD56). Positive correlations between CD4, CD8, CD68, and CD56 cells suggest coordinated immune activity within the endometrial microenvironment. Further studies with larger sample sizes and functional immune profiling are warranted to explore subtle immunological variations that may influence endometrial physiology and pathology.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of tumor necrosis factor-alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three-dimensional collagen hydrogel","authors":"Alexandria B. Tino,&nbsp;Gabi U. Dachs,&nbsp;Peter H. Sykes,&nbsp;Kenny Chitcholtan","doi":"10.1111/jog.70026","DOIUrl":"10.1111/jog.70026","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Advanced ovarian cancer involves tumor cells proliferating within ascitic fluid, which is rich in chemokines, cytokines, growth factors, and phospholipids essential for tumor progression. Tumor necrosis factor-alpha (TNF-α) and lysophosphatidic acid (LPA) are critical components that modulate ovarian tumor cell behavior in vivo, though most in vitro studies have relied on cell monolayers that do not accurately represent the tumor microenvironment. We thus investigated TNF-α and LPA effects on ovarian tumor cells cultured in collagen scaffolds, which are a model more reflective of in vivo conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Ovarian cancer cell lines SKOV-3, OVCAR-8, OVCAR-5, and OVCAR-4 were cultured in collagen I hydrogels at various concentrations of TNF-α and LPA for 2–8 days. Outcomes included cell morphology, collagen topography, secretion of vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), interleukin-6 (IL-6), and cell survival endpoints, with comparisons to collagen topography observed in ovarian tumor tissue.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Collagen I fiber topography in 3D hydrogels remodeled over time, resembling the ovarian cancer tissue. LPA significantly reduced hydrogel size in SKOV-3 cells. While LPA modestly altered VEGF secretion, both LPA and TNF-α increased IL-8 secretion over time, with TNF-α also elevating IL-6 levels. TNF-α reduced cell survival in OVCAR-8 and OVCAR-5, while LPA promoted cell growth in SKOV-3, OVCAR-8, and OVCAR-5.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>TNF-α and LPA have diverse, cell line-specific effects on ovarian cancer cells in collagen hydrogels, underscoring cell line heterogeneity and the utility of 3D models.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jog.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple nomogram prediction model for childbearing intention among female cancer survivors","authors":"Qin Xie,&nbsp;Qipeng Wei,&nbsp;Yanan Zhu,&nbsp;Ying Shi,&nbsp;Bo Chen,&nbsp;Xiaozhen Quan,&nbsp;Yanli Lan,&nbsp;Xuezhou Yang","doi":"10.1111/jog.70028","DOIUrl":"10.1111/jog.70028","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The research aims to identify the characteristics of patients with the childbearing intention after tumor diagnosis and construct a clinical prediction nomogram.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Data were obtained from the National Health and Nutrition Examination Survey (NHANES). Women diagnosed with cancer with ages ≤50 and with complete information about delivery were included. Univariate and multivariate logistic regression models were developed to identify associated factors. Predictive accuracy of the clinical prediction model was evaluated by the area under the curve (AUC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>224 and 749 participants with and without live birth after cancer diagnosis were included, respectively. In the univariate logistic regression analysis, age at tumor diagnosis, live birth before cancer diagnosis, comorbidity before cancer, family poverty income ratio, and tumor type were associated with the childbearing intention after cancer (<i>p</i> &lt;0.05). In the multivariate logistic regression analysis, age at tumor diagnosis and live birth before cancer diagnosis were correlated with fertility intention (OR 0.811, 95%CI 0.780 to 0.840; 0.155, 95%CI 0.080–0.294, respectively), others were without statistical significance probably due to the false association or indirect association (<i>p</i> &gt;0.05). A nomogram model including age at cancer diagnosis and live birth before cancer diagnosis was constructed, the AUC of which was 0.9312.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The reproductive intentions of young female cancer patients were associated with age at tumor diagnosis and live birth before cancer diagnosis. A nomogram model, including these two items with AUC 0.9312, could promptly identify patients to have children, which assists in formulating individualized treatment plans for tumors and enables timely fertility preservation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}