Vaginal metabolic profiling reveals biomarkers characteristics of high-risk HPV infection and cervical lesions

IF 1.5 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research Pub Date : 2025-08-11 DOI:10.1111/jog.70035

Xiaoling Huang, Xiaoge Li, Sijing Li, Jin Wu, Zhaoning Duan, Ming Luo, Ying Jia

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引用次数: 0

Abstract

Objective

This study aimed to identify potential biomarkers in high-risk human papillomavirus (HR-HPV) infections and related cervical lesions through the analysis of vaginal metabolic profiles.

Patients and methods

Forty cervicovaginal secretion samples were collected from healthy individuals, HR-HPV-positive individuals, low-grade squamous intraepithelial lesion patients, high-grade squamous intraepithelial lesion patients, and cervical cancer (CC) patients. All samples were analyzed using liquid chromatography–mass spectrometry (LC–MS). Metabolites with significant differences were identified using the variable importance in projection (VIP > 1) derived from the orthogonal least partial squares discriminant analysis (OPLS-DA) model and the p-value (p < 0.05) of the Student's t test. Utilizing hierarchical cluster analysis and receiver operating characteristic curve analysis, the optimal biomarkers were identified, and the diagnostic potential of these biomarkers was evaluated. Significantly enriched pathways of the differential metabolites were explored using metabolic pathway analysis.

Results

LC–MS analysis of the vaginal metabolomic profiles revealed unique molecular characteristics of different states of HR-HPV infection. Based on persistent HR-HPV infection, 3-Isochromanone increased, and DG(2:0/5-iso PGF2VI/0:0) and (4R,5S,7R,11x)-11,12-Dihydroxy-1(10)-spirovetiven-2-one 12-glucoside decreased (p < 0.05) with the aggravation of cervical lesions, suggesting their potential as biomarkers to predict various stages of cervical lesions. Additionally, analysis of metabolic pathway enrichment for differential metabolites identified the phenylalanine, tyrosine, and tryptophan biosynthesis pathways as the key metabolic pathways in the progression of cervical lesions.

Conclusion

By measuring the metabolome of vaginal secretions, specific metabolites can effectively distinguish persistent HR-HPV infections and different grades of cervical lesions, which may become an important means for the early screening and diagnosis of CC.

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查看原文本刊更多论文

阴道代谢谱揭示高危HPV感染和宫颈病变的生物标志物特征

目的本研究旨在通过分析阴道代谢谱，确定高危人乳头瘤病毒（HR-HPV）感染及相关宫颈病变的潜在生物标志物。患者与方法采集健康人群、hr - hpv阳性人群、低级别鳞状上皮内病变患者、高级别鳞状上皮内病变患者和宫颈癌（CC）患者40份宫颈阴道分泌物标本。所有样品均采用液相色谱-质谱（LC-MS）分析。使用正交最小偏二乘判别分析（OPLS-DA）模型得出的投影变量重要性（VIP > 1）和学生t检验的p值（p < 0.05）来鉴定具有显著差异的代谢物。利用分层聚类分析和受试者工作特征曲线分析，确定了最佳生物标志物，并评价了这些生物标志物的诊断潜力。利用代谢途径分析探索了差异代谢物的显著富集途径。结果LC-MS分析阴道代谢组学谱揭示了不同状态HR-HPV感染的独特分子特征。基于持续的HR-HPV感染，随着宫颈病变的加重，3-异铬罗曼酮升高，DG（2:0/5-iso PGF2VI/0:0）和(4R,5S,7R,11x)-11,12-二羟基-1(10)-螺维素-2- 1 - 12-葡萄糖苷降低（p < 0.05），提示它们有潜力作为预测宫颈病变不同阶段的生物标志物。此外，对差异代谢物的代谢途径富集分析发现，苯丙氨酸、酪氨酸和色氨酸生物合成途径是宫颈病变进展中的关键代谢途径。结论通过测定阴道分泌物代谢组，特异性代谢物可有效区分持续性HR-HPV感染和不同级别宫颈病变，可能成为CC早期筛查和诊断的重要手段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynaecology Research 医学-妇产科学

CiteScore

3.10

自引率

0.00%

发文量

376

审稿时长

3-6 weeks

期刊介绍： The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.