Journal of Microbiology最新文献_第9页

Encapsulin protein MAV2054 enhances Mycobacterium avium virulence by promoting Cdc42-dependent epithelial cell invasion. 包封蛋白MAV2054通过促进cdc42依赖性上皮细胞侵袭增强鸟分枝杆菌毒力。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-11-01 Epub Date: 2025-11-30 DOI: 10.71150/jm.2506008

Dong Ho Kim, I Jeong Jo, Min Ju Kang, Yi Seol Kim, Duyen Do Tran Huong, Kyungho Woo, Ho-Sung Park, Hwa-Jung Kim, Chul Hee Choi

{"title":"Encapsulin protein MAV2054 enhances Mycobacterium avium virulence by promoting Cdc42-dependent epithelial cell invasion.","authors":"Dong Ho Kim, I Jeong Jo, Min Ju Kang, Yi Seol Kim, Duyen Do Tran Huong, Kyungho Woo, Ho-Sung Park, Hwa-Jung Kim, Chul Hee Choi","doi":"10.71150/jm.2506008","DOIUrl":"10.71150/jm.2506008","url":null,"abstract":"Mycobacterium avium complex (MAC) organisms are widespread environmental pathogens associated with chronic pulmonary infections. Although M. avium is known to invade epithelial cells, the molecular mechanisms underlying this process remain incompletely understood. In this study, we identified a novel role for MAVRS09815 (formerly MAV2054), a family 2A encapsulin nanocompartment shell protein, in mediating bacterial adhesion, epithelial cell invasion, and in vivo virulence. We engineered a recombinant M. smegmatis strain expressing MAV2054 (Ms_2054) and an M. avium MAV2054 deletion mutant (Δ2054). Ms_2054 exhibited enhanced epithelial invasion, whereas Δ2054 showed reduced intracellular survival. Recombinant MAV2054 protein was bound directly to human epithelial cells in a dose-dependent manner. Pretreatment of host cells with cytochalasin D or vinblastine significantly inhibited bacterial internalization, indicating that MAV2054-mediated invasion is cytoskeleton-dependent. Confocal and scanning electron microscopy revealed MAV2054-dependent membrane rearrangements during infection. Pull-down assays demonstrated that MAV2054 activates Cdc42, a key regulator of actin polymerization, with reduced activation observed in Δ2054-infected cells. In a murine intratracheal infection model, the Δ2054 exhibited significantly reduced bacterial burdens and lung inflammation compared to the wild type. These findings demonstrate that MAV2054 enhances M. avium virulence by promoting epithelial cell invasion through Cdc42-dependent cytoskeletal remodeling. This study reveals a previously unrecognized role for an encapsulin-like protein in host-pathogen interactions and highlights its potential as a therapeutic target in MAC infections.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 11","pages":"e2506008"},"PeriodicalIF":3.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145634712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus crispatus KBL693 alleviates atopic dermatitis symptoms through immune modulation. 马铃薯乳杆菌KBL693通过免疫调节缓解特应性皮炎症状。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-10-31 DOI: 10.71150/jm.2509005

Seokcheon Song, Jun-Hyeong Kim, Sung Jae Jang, Eun Jung Jo, Sang Kyun Lim, GwangPyo Ko

{"title":"Lactobacillus crispatus KBL693 alleviates atopic dermatitis symptoms through immune modulation.","authors":"Seokcheon Song, Jun-Hyeong Kim, Sung Jae Jang, Eun Jung Jo, Sang Kyun Lim, GwangPyo Ko","doi":"10.71150/jm.2509005","DOIUrl":"10.71150/jm.2509005","url":null,"abstract":"Atopic dermatitis (AD) is a widespread inflammatory skin condition that affects the population worldwide. Given the implication of microbiota in AD pathogenesis, we investigated whether human-derived Lactobacillus strains could modulate AD. In this study, we identified Lactobacillus crispatus KBL693 as a probiotic candidate for AD treatment. In vitro, KBL693 suppressed mast cell degranulation and IL-4 production by T cells, suggesting its ability to attenuate key type 2 immune responses. Consistent outcomes were observed in a murine AD model, where oral administration of KBL693 alleviated disease symptoms and reduced hallmark type 2 immune markers, including plasma IgE as well as IL-4, IL-5, and IL-13 levels in skin lesions. In addition to downregulating these AD-associated immune responses, KBL693 promoted regulatory T cell (Treg) expansion in mesenteric lymph nodes, indicating its potential to restore immune balance. Collectively, these findings highlight the therapeutic potential of KBL693 for AD through enhancement of Tregs and suppression of type 2 immune responses.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 10","pages":"e2509005"},"PeriodicalIF":3.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM structure of the glycosylated protein CgeA in the crust of Bacillus subtilis endospores. 枯草芽孢杆菌内生孢子外壳糖化蛋白CgeA的低温电镜结构。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-10-31 DOI: 10.71150/jm.2504013

Migak Park, Doyeon Kim, Yeongjin Baek, Eunbyul Jo, Jaekyung Hyun, Nam-Chul Ha

{"title":"Cryo-EM structure of the glycosylated protein CgeA in the crust of Bacillus subtilis endospores.","authors":"Migak Park, Doyeon Kim, Yeongjin Baek, Eunbyul Jo, Jaekyung Hyun, Nam-Chul Ha","doi":"10.71150/jm.2504013","DOIUrl":"10.71150/jm.2504013","url":null,"abstract":"The Bacillus subtilis spore crust is an exceptionally robust proteinaceous layer that protects spores under extreme environmental conditions. Among its key components, CgeA, a glycosylation-associated protein, plays a critical role in modifying crust properties through its glycosylated moiety, enhancing spore dispersal in aqueous environments. In this study, we present the high-resolution cryo-electron microscopy structure of the core region of CgeA at 3.05 Å resolution, revealing a doughnut-like hexameric assembly. The N-terminal regions are disordered, whereas the C-terminal region forms the core of the hexamer. Although the loop containing Thr112 was not resolved in the density map, its location can be inferred from surrounding residues, suggesting that Thr112 is situated on the exposed surface of the hexamer. On the opposite face, a distinct electrostatic pattern is observed, featuring a negatively charged central pore and a positively charged outer surface. Modeling and biochemical studies with the putative glycosyltransferase CgeB provide insights into how the glycosyl group is transferred to Thr112. This study offers a molecular-level understanding of the assembly, glycosylation, and environmental adaptability of the B. subtilis spore crust, with valuable implications for controlling spore formation in industrial applications.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 10","pages":"e2504013"},"PeriodicalIF":3.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-based classification of Paraniabella aurantiaca gen. nov., sp. nov., isolated from soil and taxonomic reclassification of five species within the genus Niabella. 土壤分离金蝇属（Paraniabella aurantiaca gen. nov., spnov .）的基因组分类及五种Niabella属的分类再分类。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-10-31 DOI: 10.71150/jm.2505005

Yong-Seok Kim, Yerang Yang, Miryung Kim, Do-Hoon Lee, Chang-Jun Cha

{"title":"Genome-based classification of Paraniabella aurantiaca gen. nov., sp. nov., isolated from soil and taxonomic reclassification of five species within the genus Niabella.","authors":"Yong-Seok Kim, Yerang Yang, Miryung Kim, Do-Hoon Lee, Chang-Jun Cha","doi":"10.71150/jm.2505005","DOIUrl":"10.71150/jm.2505005","url":null,"abstract":"A Gram-stain-negative, aerobic, non-motile, rod-shaped, and orange-pigmented bacterium, designated CJ426T, was isolated from ginseng soil in Anseong, Korea. Strain CJ426T grew optimally on Reasoner's 2A agar at 30°C and pH 7.0 in the absence of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ426T belonged to the family Chitinophagaceae and had the highest sequence similarity with Niabella hibiscisoli KACC 18857T (98.7%). The 16S rRNA gene sequence similarities with other members of the genus Niabella ranged from 92.3% to 98.1%. Phylogenomic analyses and overall genomic relatedness indices, including average nucleotide identity, average amino acid identity, and the percentage of conserved proteins values, supported the classification of strain CJ426T as a representative of a novel genus within the family Chitinophagaceae. Furthermore, genome-based analyses suggested that five members of the genus Niabella, including N. aquatica, N. defluvii, N. ginsengisoli, N. hibiscisoli, and, N. yanshanensis, should be separated from other Niabella species and be assigned as a novel genus. The major isoprenoid quinone of strain CJ426T was menaquinone-7 (MK-7). The predominant polar lipids were phosphatidylethanolamine and six unidentified aminolipids. The major fatty acids were iso-C15:0, iso-C15:1 G, and iso-C17:0 3-OH. The genome of strain CJ426T was 6.3 Mbp in size, consisting of three contigs, with a G + C content of 41.9%. Based on a polyphasic taxonomic approach, strain CJ426T represents a novel genus and species within the family Chitinophagaceae, for which the name Paraniabella aurantiaca gen. nov., sp. nov. is proposed. The type strain is CJ426T (= KACC 23908T = JCM 37728T).","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 10","pages":"e2505005"},"PeriodicalIF":3.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13576996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pycnogenol reduces the expression of P. aeruginosa T3SS and inflammatory response in NCI-H292 cells. 碧萝芷酚可降低铜绿假单胞菌T3SS的表达和NCI-H292细胞的炎症反应。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-09-19 DOI: 10.71150/jm.2503004

Seung-Ho Kim, Da Yun Seo, Sang-Bae Han, Un-Hwan Ha, Ji-Won Park, Kyung-Seop Ahn

{"title":"Pycnogenol reduces the expression of P. aeruginosa T3SS and inflammatory response in NCI-H292 cells.","authors":"Seung-Ho Kim, Da Yun Seo, Sang-Bae Han, Un-Hwan Ha, Ji-Won Park, Kyung-Seop Ahn","doi":"10.71150/jm.2503004","DOIUrl":"10.71150/jm.2503004","url":null,"abstract":"Nosocomial infections caused by Pseudomonas aeruginosa (P. aeruginosa) have become increasingly common, particularly among immunocompromised individuals, who experience high mortality rates and prolonged treatment durations due to the limited availability of effective therapies. In this study, we screened for anti-ExoS compounds targeting P. aeruginosa and identified pycnogenol (PYC) as a potent inhibitor of the type III secretion system (T3SS), a major virulence mechanism responsible for the translocation of effectors such as ExoS. Using ELISA, western blotting, and real-time PCR analyses in both P. aeruginosa and infected H292 cells, we found that PYC significantly reduced T3SS activity. Mechanistically, PYC suppressed the transcription of T3SS-related genes by downregulating exsA expression in P. aeruginosa. Furthermore, pretreatment with PYC attenuated the cytotoxic effects and reduced the expression of proinflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-18 (IL-18), in P. aeruginosa-infected H292 cells. These effects were associated with the inhibition of NF-κB signaling and inflammasome activation. Taken together, our findings suggest that PYC may serve as a promising therapeutic candidate against P. aeruginosa infections by targeting T3SS-mediated virulence and modulating host inflammatory responses.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":" ","pages":"2503004"},"PeriodicalIF":3.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Corrigendum: Staphylococcus parequorum sp. nov. and Staphylococcus halotolerans sp. nov., isolated from traditional Korean soybean foods. 撤回：勘误：从韩国传统大豆食品中分离的嗜盐葡萄球菌和嗜盐葡萄球菌。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-10-22 DOI: 10.71150/jm.2510100

Ju Hye Baek, Dong Min Han, Dae Gyu Choi, Chae Yeong Moon, Jae Kyeong Lee, Chul-Hong Kim, Jung-Woong Kim, Che Ok Jeon

引用次数: 0

Mannose phosphotransferase system subunit IID of Streptococcus mutans elicits maturation and activation of dendritic cells. 突变链球菌的甘露糖磷酸转移酶系统亚基IID诱导树突状细胞的成熟和活化。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-10-31 DOI: 10.71150/jm.2505014

Sungho Jeong, Chaeyeon Park, Dongwook Lee, Hyun Jung Ji, Ho Seong Seo, Cheol-Heui Yun, Jintaek Im, Seung Hyun Han

{"title":"Mannose phosphotransferase system subunit IID of Streptococcus mutans elicits maturation and activation of dendritic cells.","authors":"Sungho Jeong, Chaeyeon Park, Dongwook Lee, Hyun Jung Ji, Ho Seong Seo, Cheol-Heui Yun, Jintaek Im, Seung Hyun Han","doi":"10.71150/jm.2505014","DOIUrl":"10.71150/jm.2505014","url":null,"abstract":"Streptococcus mutans is a Gram-positive pathogen that causes dental caries and subsequent pulpal infection leading to pulpitis. Although dendritic cells (DCs) are known to be involved in disease progression and immune responses during S. mutans infection, little is known about the component of S. mutans responsible for the DC responses. Although the mannose phosphotransferase system (Man-PTS) is the primary sugar transporter of S. mutans, it is also a potential virulence factor. Since Man-PTS subunit IID (ManIID) embedded on the bacterial membrane is indispensable for Man-PTS function, we investigated its role in the maturation and activation of DCs stimulated with a ManIID-deficient strain (Δpts) of S. mutans and recombinant ManIID (rManIID) protein. When mouse bone marrow-derived DCs were treated with heat-killed S. mutans wild-type (WT) or Δpts, bacterial adherence and internalization of Δpts were lower than those of WT. Moreover, the heat-killed S. mutans Δpts strain was inferior to the wild-type in inducing expression of phenotypic maturation markers, such as CD80, CD86, MHC-I, and MHC-II, and proinflammatory cytokine, IL-6. In line with the trends in marker expression, the endocytic capacity of DCs treated with the Δpts strain was comparable to that of untreated DCs whereas DCs treated with the WT strain dose-dependently lost their endocytic capacity. Furthermore, rManIID dose-dependently promoted both phenotypic maturation marker expression and IL-6 production by DCs. Collectively, these results demonstrate that ManIID plays a crucial role in the adhesion and internalization of S. mutans into DCs and is one of the major immune-stimulating agents responsible for maturation and activation of DCs during S. mutans infection.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 10","pages":"e2505014"},"PeriodicalIF":3.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum: Staphylococcus parequorum sp. nov. and Staphylococcus halotolerans sp. nov., isolated from traditional Korean soybean foods. 勘误：从韩国传统大豆食品中分离出的嗜盐葡萄球菌和嗜盐葡萄球菌。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-10-23 DOI: 10.71150/jm.2510101

Ju Hye Baek, Dong Min Han, Dae Gyu Choi, Chae Yeong Moon, Jae Kyeong Lee, Chul-Hong Kim, Jung-Woong Kim, Che Ok Jeon

引用次数: 0

Structural analysis of dual specificity phosphatases, the only type of protein tyrosine phosphatases found in humans and across diverse microorganisms. 双特异性磷酸酶的结构分析，这是人类和多种微生物中发现的唯一类型的蛋白酪氨酸磷酸酶。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-10-31 DOI: 10.71150/jm.2506006

Bonsu Ku

{"title":"Structural analysis of dual specificity phosphatases, the only type of protein tyrosine phosphatases found in humans and across diverse microorganisms.","authors":"Bonsu Ku","doi":"10.71150/jm.2506006","DOIUrl":"10.71150/jm.2506006","url":null,"abstract":"Dual specificity phosphatases (DUSPs), a subfamily of the protein tyrosine phosphatase (PTP) family, dephosphorylate not only phosphotyrosine but also phosphoserine and phosphothreonine residues. Beyond the 26 members of this family in humans, DUSPs represent the only type of PTPs found across a wide range of microorganisms, including bacteria, archaea, and viruses. This review presents a comprehensive structural analysis of human and microbial DUSPs. These proteins commonly share core features, such as a typical DUSP fold, shallow active site pocket, signature active site motif known as the P-loop, and conserved aspartate residue that acts as a general acid/base. However, DUSPs from diverse microorganisms also display unique structural and functional characteristics. Pseudomonas aeruginosa TpbA is the only bacterial DUSP identified to date, while a second candidate was proposed in this review. Archaeal DUSPs are hyperthermostable, contain a unique motif in their P-loops, and employ dual general acid/base residues. Poxviral DUSPs are characterized by the formation of domain-swapped homodimers. The presence of DUSPs across all domains of life and viruses, along with their low specificity for phosphorylated amino acids and structural similarity to classical PTPs, suggests that DUSPs represent the ancestral form of PTPs.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 10","pages":"e2506006"},"PeriodicalIF":3.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection system- and strain-dependent diversity of de novo [PSI+] prion generation and phenotypes in Saccharomyces cerevisiae. 酿酒酵母中新生[PSI+]朊病毒生成和表型的系统和菌株依赖性多样性检测。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-10-01 Epub Date: 2025-09-18 DOI: 10.71150/jm.2506009

Moonil Son

{"title":"Detection system- and strain-dependent diversity of de novo [PSI+] prion generation and phenotypes in Saccharomyces cerevisiae.","authors":"Moonil Son","doi":"10.71150/jm.2506009","DOIUrl":"10.71150/jm.2506009","url":null,"abstract":"Yeast prion [PSI+], an amyloid form of the translation termination factor Sup35p/eRF3, causes translational stop codon readthrough by sequestering functional Sup35p. This unique phenotype may be analyzed via [PSI+]-suppressible nonsense alleles, and has greatly contributed to the advancement in yeast prion research. For comparing canonical reporters, like chromosomal ade1-14 or ade2-1, and plasmid-borne ura3-14, the de novo generation and characteristics of [PSI+] was investigated across common yeast laboratory strains (BY4741, 74D-694, and 779-6A). The results showed significant variability in [PSI+] induction frequency among strains. [PSI+] was successfully induced in BY4741 and frequently in 74D-694 (via Ade+ selection), but not in 779-6A. Notably, [PSI+] clones, even from identical genetic backgrounds, displayed vastly different nonsense suppression phenotypes depending on the reporter allele used; resulting in diverse growth patterns and suppression levels. Quantitative analyses revealed that prion seed counts fluctuated significantly based on the detection allele and observed phenotype. Furthermore, Sup35p aggregate visualization revealed distinct structural patterns between BY4741 and 74D-694, indicating strain-specific differences. Transferring [PIN+] prion variants from different strains into a common [psi-][pin-] background yielded similar [PSI+] inducibility and seed numbers, suggesting that the observed phenotypic and quantitative diversities of [PSI+] prions stem primarily from the interplay between the specific reporter detection system and the host strain's genetic background rather than solely from inherent differences in the initial [PIN+] prion or fundamental changes in the [PSI+] protein itself. This study underscores the crucial need to consider both the detection methodology and host genetic context for accurate prion variant characterization.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":" ","pages":"e2506009"},"PeriodicalIF":3.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0