肠炎沙门氏菌 EnvE 是一种外膜脂蛋白,其基因表达导致病毒基因 msgA 的转录抑制。

IF 3.3 4区 生物学 Q2 MICROBIOLOGY
Journal of Microbiology Pub Date : 2024-11-01 Epub Date: 2024-11-15 DOI:10.1007/s12275-024-00183-4
Sinyeon Kim, Yong Heon Lee
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引用次数: 0

摘要

Typhimurium 肠炎沙门氏菌的 envE 基因在沙门氏菌致病性岛-11(SPI-11)中编码,紧邻致病基因 msgA(巨噬细胞存活基因 A)的上游,处于相同的转录方向。迄今为止,envE 的特征和作用在很大程度上仍未被探索。在本研究中,我们利用蔗糖梯度超速离心法证明了 EnvE(一种预测的脂蛋白)定位于外膜上。在氧化应激条件下,envE 的转录被抑制,而 msgA 的转录被诱导,这表明这两个相邻基因的 mRNA 水平之间存在反相关性。重要的是,无论是否存在氧化应激,envE 失活都会导致 msgA 的组成型转录。此外,用质粒携带的 envE 对 envE 突变体进行反式互补也不能阻止 msgA 的诱导转录,这表明 envE 的功能是顺式调节元件而不是反式作用因子。我们进一步发现,envE 的失活和互补都能确保 msgA 的表达,从而赋予野生型水平的抗氧化压力。我们的数据表明,肠杆菌 envE 基因编码一种外膜脂蛋白,它的转录以顺式作用方式抑制 msgA 的表达,可能是通过转录干扰,但具体的分子细节尚不清楚。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Salmonella enterica EnvE is an Outer Membrane Lipoprotein and Its Gene Expression Leads to Transcriptional Repression of the Virulence Gene msgA.

The envE gene of Salmonella enterica serovar Typhimurium is encoded within Salmonella Pathogenicity Island-11 (SPI-11) and is located immediately upstream of the virulence gene msgA (macrophage survival gene A) in the same transcriptional orientation. To date, the characteristics and roles of envE remain largely unexplored. In this study, we show that EnvE, a predicted lipoprotein, is localized on the outer membrane using sucrose gradient ultracentrifugation. Under oxidative stress conditions, envE transcription is suppressed, while msgA transcription is induced, indicating an inverse correlation between the mRNA levels of the two neighboring genes. Importantly, inactivation of envE leads to constitutive transcription of msgA regardless of the presence of oxidative stress. Moreover, trans-complementation of the envE mutant with a plasmid-borne envE fails to prevent the induction of msgA transcription, suggesting that envE functions as a cis-regulatory element rather than a trans-acting factor. We further show that both inactivation and complementation of envE confer wild-type levels of resistance to oxidative stress by ensuring the expression of msgA. Our data suggest that the S. enterica envE gene encodes an outer membrane lipoprotein, and its transcription represses msgA expression in a cis-acting manner, probably by transcriptional interference, although the exact molecular details are yet unclear.

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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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