Journal of Microbiology最新文献

Recent Advances of Nipah Virus Disease: Pathobiology to Treatment and Vaccine Advancement 尼帕病毒病的最新进展：从病理生物学到治疗和疫苗进展

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-09-18 DOI: 10.1007/s12275-024-00168-3

Sagnik Saha, Manojit Bhattacharya, Sang-Soo Lee, Chiranjib Chakraborty

{"title":"Recent Advances of Nipah Virus Disease: Pathobiology to Treatment and Vaccine Advancement","authors":"Sagnik Saha, Manojit Bhattacharya, Sang-Soo Lee, Chiranjib Chakraborty","doi":"10.1007/s12275-024-00168-3","DOIUrl":"https://doi.org/10.1007/s12275-024-00168-3","url":null,"abstract":"The zoonotic infection of the Nipah virus (NiV) has yet again appeared in 2023 in Kerala state, India. The virus, which has a mortality rate ranging from about 40 to 70%, has already infected India five times, the first being in 2001. The current infection is the sixth virus outbreak in the Indian population. In 1998, the first NiV infection was noted in one village in Malaysia. After that, outbreaks from other South and Southeast Asian countries have been reported periodically. It can spread between humans through contact with body fluids. Therefore, it is unlikely to generate a new pandemic. However, there is a considerable knowledge gap in the different areas of NiV. To date, no approved vaccines or treatments have been available. To fulfil the knowledge gap, the review article provided a detailed overview of the genome and genome-encoded proteins, epidemiology, transmission, pathobiology, immunobiology, diagnosis, prevention and control measures, therapeutics (monoclonal antibodies and drug molecules), and vaccine advancement of the emerging and deadly pathogen. The advanced information will help researchers to develop safe and effective NiV vaccine and treatment regimens worldwide.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome-Mucosal Immunity Nexus: Driving Forces in Respiratory Disease Progression. 微生物组-粘膜免疫关系：呼吸系统疾病进展的驱动力。

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-09-06 DOI: 10.1007/s12275-024-00167-4

Young Chae Park, Soo Yeon Choi, Yunah Cha, Hyeong Won Yoon, Young Min Son

引用次数: 0

Unexpected Requirement of Small Amino Acids at Position 183 for DNA Binding in the Escherichia coli cAMP Receptor Protein. 大肠杆菌 cAMP 受体蛋白中 183 位的小氨基酸对 DNA 结合的意外需求。

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-09-06 DOI: 10.1007/s12275-024-00169-2

Marcus Carranza, Amanda Rea, Daisy Pacheco, Christian Montiel, Jin Park, Hwan Youn

{"title":"Unexpected Requirement of Small Amino Acids at Position 183 for DNA Binding in the Escherichia coli cAMP Receptor Protein.","authors":"Marcus Carranza, Amanda Rea, Daisy Pacheco, Christian Montiel, Jin Park, Hwan Youn","doi":"10.1007/s12275-024-00169-2","DOIUrl":"https://doi.org/10.1007/s12275-024-00169-2","url":null,"abstract":"The Escherichia coli cAMP receptor protein (CRP) relies on the F-helix, the recognition helix of the helix-turn-helix motif, for DNA binding. The importance of the CRP F-helix in DNA binding is well-established, yet there is little information on the roles of its non-base-contacting residues. Here, we show that a CRP F-helix position occupied by a non-base-contacting residue Val183 bears an unexpected importance in DNA binding. Codon randomization and successive in vivo screening selected six amino acids (alanine, cysteine, glycine, serine, threonine, and valine) at CRP position 183 to be compatible with DNA binding. These amino acids are quite different in their amino acid properties (polar, non-polar, hydrophobicity), but one commonality is that they are all relatively small. Larger amino acid substitutions such as histidine, methionine, and tyrosine were made site-directedly and showed to have no detectable DNA binding, further supporting the requirement of small amino acids at CRP position 183. Bioinformatics analysis revealed that small amino acids (92.15% valine and 7.75% alanine) exclusively occupy the position analogous to CRP Val183 in 1,007 core CRP homologs, consistent with our mutant data. However, in extended CRP homologs comprising 3700 proteins, larger amino acids could also occupy the position analogous to CRP Val183 albeit with low occurrence. Another bioinformatics analysis suggested that large amino acids could be tolerated by compensatory small-sized amino acids at their neighboring positions. A full understanding of the unexpected requirement of small amino acids at CRP position 183 for DNA binding entails the verification of the hypothesized compensatory change(s) in CRP.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Gut Microbiota Mediates the Protective Effects of Spironolactone on Myocardial Infarction. 肠道微生物群介导螺内酯对心肌梗死的保护作用

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-09-03 DOI: 10.1007/s12275-024-00164-7

Lu Li, Jian-Yong Sun, Yu-Lin Li, Shi-Wei Zhu, Sheng-Zhong Duan

{"title":"The Gut Microbiota Mediates the Protective Effects of Spironolactone on Myocardial Infarction.","authors":"Lu Li, Jian-Yong Sun, Yu-Lin Li, Shi-Wei Zhu, Sheng-Zhong Duan","doi":"10.1007/s12275-024-00164-7","DOIUrl":"https://doi.org/10.1007/s12275-024-00164-7","url":null,"abstract":"Myocardial infarction (MI) is a type of cardiovascular disease that influences millions of human beings worldwide and has a great rate of mortality and morbidity. Spironolactone has been used as a critical drug for the treatment of cardiac failure and it ameliorates cardiac dysfunction post-MI. Despite these findings, whether there is a relationship between the therapeutic effects of spironolactone and the gut microorganism after MI has not been determined. In our research, we used male C57BL/6 J mice to explore whether the gut microbiota mediates the beneficial function of spironolactone after myocardial infarction. We demonstrated that deletion of the gut microbiota eliminated the beneficial function of spironolactone in MI mice, displaying exacerbated cardiac dysfunction, cardiac infarct size. In addition, the gut microbiota was altered by spironolactone after sham or MI operation in mice. We also used male C57BL/6 J mice to investigate the function of a probiotic in the myocardial infarction. In summary, our findings reveal a precious role of the gut flora in the therapeutic function of spironolactone on MI.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Adaptability and Roles in Ammonia Oxidation of Aerobic Ammonia-Oxidizing Microorganisms in the Surface Sediments of East China Sea. 东海表层沉积物中好氧氨氧化微生物的环境适应性及其在氨氧化过程中的作用

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-08-30 DOI: 10.1007/s12275-024-00166-5

Wenhui Li, Yu Zhen, Yuhong Yang, Daling Wang, Hui He

{"title":"Environmental Adaptability and Roles in Ammonia Oxidation of Aerobic Ammonia-Oxidizing Microorganisms in the Surface Sediments of East China Sea.","authors":"Wenhui Li, Yu Zhen, Yuhong Yang, Daling Wang, Hui He","doi":"10.1007/s12275-024-00166-5","DOIUrl":"https://doi.org/10.1007/s12275-024-00166-5","url":null,"abstract":"This study investigated the community characteristics and environmental influencing factors of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in the surface sediments of the East China Sea. The research found no consistent pattern in the richness and diversity of AOA and AOB with respect to the distance from the shore, indicating a complex interplay of factors. The expression levels of AOA amoA gene and AOB amoA gene in the surface sediments of the East China Sea ranged from 4.49 × 102 to 2.17 × 106 copies per gram of sediment and from 6.6 × 101 to 7.65 × 104 copies per gram of sediment, respectively. Salinity (31.77 to 34.53 PSU) and nitrate concentration (1.51 to 10.12 μmol/L) were identified as key environmental factors significantly affecting the AOA community, while salinity and temperature (13.71 to 19.50 °C) were crucial for the AOB community. The study also found that AOA, dominated by the Nitrosopumilaceae family, exhibited higher gene expression levels than AOB, suggesting a more significant role in ammonia oxidation. The expression of AOB was sensitive to multiple environmental factors, indicating a responsive role in nitrogen cycles and ecosystem health. The findings contribute to a better understanding of the biogeochemical processes and ecological roles of ammonia-oxidizing microorganisms in marine sediments.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection Dynamics of Dengue Virus in Caco-2 Cells Depending on Its Differentiation Status. 登革病毒在 Caco-2 细胞中的感染动态取决于其分化状态。

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-08-30 DOI: 10.1007/s12275-024-00161-w

Jayoung Nam, Jisu Lee, Geon A Kim, Seung-Min Yoo, Changhoon Park, Myung-Shin Lee

{"title":"Infection Dynamics of Dengue Virus in Caco-2 Cells Depending on Its Differentiation Status.","authors":"Jayoung Nam, Jisu Lee, Geon A Kim, Seung-Min Yoo, Changhoon Park, Myung-Shin Lee","doi":"10.1007/s12275-024-00161-w","DOIUrl":"https://doi.org/10.1007/s12275-024-00161-w","url":null,"abstract":"Dengue virus (DENV), from the Flaviviridae family, is the causative agent of dengue fever and poses a significant global health challenge. The virus primarily affects the vascular system and liver; however, a growing body of evidence suggests its involvement in the gastrointestinal (GI) tract, contributing to clinical symptoms such as abdominal pain, vomiting, and diarrhea. However, the mechanisms underlying DENV infection in the digestive system remain largely unexplored. Prior research has detected viral RNA in the GI tissue of infected animals; however, whether the dengue virus can directly infect human enterocytes remains unclear. In this study, we examine the infectivity of human intestinal cell lines to the dengue virus and their subsequent response. We report that the Caco-2 cell line, a model of human enterocytes, is susceptible to infection and capable of producing viruses. Notably, differentiated Caco-2 cells exhibited a lower infection rate yet a higher level of virus production than their undifferentiated counterparts. These findings suggest that human intestinal cells are a viable target for the dengue virus, potentially elucidating the GI symptoms observed in dengue fever and offering a new perspective on the pathogenetic mechanisms of the virus.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Analyses of DNA Double-Strand Break Repair Pathways in Tandem Arrays of HXT Genes of Saccharomyces Cerevisiae. 勘误：对酿酒酵母 HXT 基因串联阵列中 DNA 双链断裂修复途径的分析

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-08-26 DOI: 10.1007/s12275-024-00127-y

Ju-Hee Choi, Ye-Seul Lim, Min-Ku Kim, Sung-Ho Bae

引用次数: 0

Erratum: Effects of the Loss of Mismatch Repair Genes on Single-Strand Annealing Between Divergent Sequences in Saccharomyces cerevisiae. 勘误：错配修复基因缺失对酿酒酵母中不同序列间单链退火的影响

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-08-26 DOI: 10.1007/s12275-024-00126-z

Ye-Seul Lim, Ju-Hee Choi, Kyu-Jin Ahn, Min-Ku Kim, Sung-Ho Bae

引用次数: 0

FgVAC1 is an Essential Gene Required for Golgi-to-Vacuole Transport and Fungal Development in Fusarium graminearum. FgVAC1 是禾谷镰刀菌高尔基体到液泡转运和真菌发育所需的重要基因

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-08-01 Epub Date: 2024-07-30 DOI: 10.1007/s12275-024-00160-x

Sieun Kim, Jiyeun Park, You-Kyoung Han, Hokyoung Son

引用次数: 0

RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA. RapB 在竹荪中调控细胞粘附和迁移，与 RapA 相似。

IF 3.3 4区生物学

Journal of Microbiology Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1007/s12275-024-00143-y

Uri Han, Nara Han, Byeonggyu Park, Taeck Joong Jeon

{"title":"RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA.","authors":"Uri Han, Nara Han, Byeonggyu Park, Taeck Joong Jeon","doi":"10.1007/s12275-024-00143-y","DOIUrl":"10.1007/s12275-024-00143-y","url":null,"abstract":"Ras small GTPases act as molecular switches in various cellular signaling pathways, including cell migration, proliferation, and differentiation. Three Rap proteins are present in Dictyostelium; RapA, RapB, and RapC. RapA and RapC have been reported to have opposing functions in the control of cell adhesion and migration. Here, we investigated the role of RapB, a member of the Ras GTPase subfamily in Dictyostelium, focusing on its involvement in cell adhesion, migration, and developmental processes. This study revealed that RapB, similar to RapA, played a crucial role in regulating cell morphology, adhesion, and migration. rapB null cells, which were generated by CRISPR/Cas9 gene editing, displayed altered cell size, reduced cell-substrate adhesion, and increased migration speed during chemotaxis. These phenotypes of rapB null cells were restored by the expression of RapB and RapA, but not RapC. Consistent with these results, RapB, similar to RapA, failed to rescue the phenotypes of rapC null cells, spread morphology, increased cell adhesion, and decreased migration speed during chemotaxis. Multicellular development of rapB null cells remained unaffected. These results suggest that RapB is involved in controlling cell morphology and cell adhesion. Importantly, RapB appears to play an inhibitory role in regulating the migration speed during chemotaxis, possibly by controlling cell-substrate adhesion, resembling the functions of RapA. These findings contribute to the understanding of the functional relationships among Ras subfamily proteins.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0