Inhibition of Virulence Associated Traits by β-Sitosterol Isolated from Hibiscus rosa-sinensis Flowers Against Candida albicans: Mechanistic Insight and Molecular Docking Studies.

IF 3.3 4区生物学 Q2 MICROBIOLOGY

Journal of Microbiology Pub Date : 2024-11-06 DOI:10.1007/s12275-024-00174-5

Pallvi Mohana, Atamjit Singh, Farhana Rashid, Sharabjit Singh, Kirandeep Kaur, Rupali Rana, Preet Mohinder Singh Bedi, Neena Bedi, Rajinder Kaur, Saroj Arora

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引用次数: 0

Abstract

The emerging drug resistance and lack of safer and more potent antifungal agents make Candida infections another hot topic in the healthcare system. At the same time, the potential of plant products in developing novel antifungal drugs is also in the limelight. Considering these facts, we have investigated the different extracts of the flowers of Hibiscus rosa-sinensis of the Malvaceae family for their antifungal efficacy against five different pathogenic Candida strains. Among the various extracts, the chloroform extract showed the maximum zone of inhibition (26.6 ± 0.5 mm) against the Candida albicans strain. Furthermore, the chloroform fraction was isolated, and a sterol compound was identified as β-sitosterol. Mechanistic studies were conducted to understand the mechanism of action, and the results showed that β-sitosterol has significant antifungal activity and is capable of interrupting biofilm formation and acts by inhibiting ergosterol biosynthesis in Candida albicans cells. Microscopic and molecular docking studies confirmed these findings. Overall, the study validates the antifungal efficacy of Candida albicans due to the presence of β-sitosterol which can act as an effective constituent for antifungal drug development individually or in combination.

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从木槿花中分离的β-谷甾醇对白色念珠菌病毒相关性状的抑制：机理洞察和分子对接研究。

新出现的耐药性以及缺乏更安全、更有效的抗真菌药物，使念珠菌感染成为医疗系统的另一个热门话题。与此同时，植物产品在开发新型抗真菌药物方面的潜力也备受关注。有鉴于此，我们研究了锦葵科植物木槿花的不同提取物对五种不同致病性念珠菌菌株的抗真菌功效。在各种提取物中，氯仿提取物对白色念珠菌菌株的抑制面积最大（26.6 ± 0.5 毫米）。此外，还分离出了氯仿馏分，并鉴定出一种甾醇化合物，即β-谷甾醇。结果表明，β-谷甾醇具有显著的抗真菌活性，能够阻断生物膜的形成，并通过抑制白色念珠菌细胞中麦角甾醇的生物合成发挥作用。显微和分子对接研究证实了这些发现。总之，这项研究验证了β-谷甾醇对白色念珠菌的抗真菌功效，β-谷甾醇可作为一种有效成分单独或联合用于抗真菌药物的开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Microbiology 生物-微生物学

CiteScore

5.70

自引率

3.30%

发文量

审稿时长

3 months

期刊介绍： Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.