Journal of Microbiology最新文献_第7页

Effects of sequencing platforms on the profiling of root mycorrhizal communities in Pinus densiflora. 测序平台对密松根菌群落图谱的影响。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-01-01 Epub Date: 2026-01-31 DOI: 10.71150/jm.2509008

Ki Hyeong Park, Seung-Yoon Oh, Shinnam Yoo, Yoonhee Cho, Ji Seon Kim, Chang Wan Seo, Chang Sun Kim, Young Woon Lim

{"title":"Effects of sequencing platforms on the profiling of root mycorrhizal communities in Pinus densiflora.","authors":"Ki Hyeong Park, Seung-Yoon Oh, Shinnam Yoo, Yoonhee Cho, Ji Seon Kim, Chang Wan Seo, Chang Sun Kim, Young Woon Lim","doi":"10.71150/jm.2509008","DOIUrl":"10.71150/jm.2509008","url":null,"abstract":"Next-generation sequencing (NGS) has become a powerful and efficient tool for surveying mycorrhizal mycobiome diversity, surpassing classical methods in accuracy and throughput. Long-read NGS techniques are increasingly applied under the assumption that they provide better taxonomic resolution, yet their use often lacks a balanced evaluation against the established strengths and limitations of widely used short-read NGS technologies. This study compares Illumina MiSeq and PacBio Sequel platforms in analyzing the mycorrhizal mycobiome of Pinus densiflora roots, focusing on how sequencing platforms and database choice influence taxonomic resolution and diversity patterns. Both platforms detected mycorrhizal taxa with similar taxonomic resolution, recovering nearly all taxa previously reported from pine roots. Most mycorrhizal taxa were shared between datasets, although several taxa were detected exclusively by one platform. In terms of diversity, the short-read dataset showed higher diversity due to greater sequencing depth, whereas the long-read dataset offered improved identification of rare or closely related taxa owing to longer sequence information. Moreover, supplementing reference databases with locally derived sequences enhanced taxonomic resolution and the detection of native taxa in both approaches, with a stronger effect for the long-read dataset. Overall, our results emphasize that short- and long-read sequencing each have distinct advantages for mycorrhizal community analysis, and that the use of curated local reference databases is essential to maximize taxonomic resolution and improve the detection of regionally unique taxa.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 1","pages":"e2509008"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic anti-obesity effects of Bifidobacterium breve BR3 and Lactiplantibacillus plantarum LP3 via coordinated regulation of lipid metabolism and gut microbiota. 短双歧杆菌BR3和植物乳杆菌LP3通过协调调节脂质代谢和肠道微生物群的协同抗肥胖作用

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-12-01 Epub Date: 2025-12-31 DOI: 10.71150/jm.2511001

Misun Yun, Dooheon Son, Namhee Kim, Se Hee Lee, Eunbee Cho, Sanghyun Lim

{"title":"Synergistic anti-obesity effects of Bifidobacterium breve BR3 and Lactiplantibacillus plantarum LP3 via coordinated regulation of lipid metabolism and gut microbiota.","authors":"Misun Yun, Dooheon Son, Namhee Kim, Se Hee Lee, Eunbee Cho, Sanghyun Lim","doi":"10.71150/jm.2511001","DOIUrl":"10.71150/jm.2511001","url":null,"abstract":"The global rise in obesity and its associated metabolic complications underscores the urgent need for safe and effective interventions. This study investigated the anti-obesity efficacy of a probiotic mixture containing Bifidobacterium breve BR3 and Lactiplantibacillus plantarum LP3 in C57BL/6 mice with high-fat diet (HFD)-induced obesity. After obesity was established by feeding a 60% kcal HFD, the probiotic mixture was administered orally for 4 weeks. Compared with the control group, mice receiving the L. plantarum LP3 and B. breve BR3 mixture exhibited significant reductions in body weight and total fat mass, as assessed by Dual-energy X-ray Absorptiometry (DXA) and Echo Magnetic Resonance Imaging (EchoMRI). The probiotic treatment also lowered serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and glucose levels, and attenuated lipid accumulation in both hepatic and epididymal adipose tissues. Transcriptomic profiling revealed upregulation of lipolytic genes (Sirt1, Pparα) and downregulation of lipogenic genes (Srebp1c, Fas), suggesting that the probiotic mixture promotes lipid catabolism while suppressing lipid synthesis. Additionally, serum adipokine levels were favorably modulated, indicating improved metabolic homeostasis. Gut microbiota analysis demonstrated an increased relative abundance of beneficial genera, including Akkermansia and Bacteroides, highlighting a microbiome-mediated contribution to the observed metabolic benefits. Overall, our findings indicate that the combined administration of Lactiplantibacillus plantarum LP3 and Bifidobacterium breve BR3 exerts multi-faceted anti-obesity effects by enhancing lipolysis, regulating lipid metabolism, and restoring a healthy gut microbial balance. This probiotic mixture represents a promising therapeutic approach for managing obesity and related metabolic disorders.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 12","pages":"e2511001"},"PeriodicalIF":3.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cinchonidine induces muscle weakness by inhibiting insulin-mediated IRS-1-AKT signaling pathway. Cinchonidine通过抑制胰岛素介导的IRS-1-AKT信号通路诱导肌无力。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-12-01 Epub Date: 2025-12-31 DOI: 10.71150/jm.2511017

Mi Ran Byun, Sang Hoon Joo, Young-Suk Jung, Joon-Seok Choi

{"title":"Cinchonidine induces muscle weakness by inhibiting insulin-mediated IRS-1-AKT signaling pathway.","authors":"Mi Ran Byun, Sang Hoon Joo, Young-Suk Jung, Joon-Seok Choi","doi":"10.71150/jm.2511017","DOIUrl":"10.71150/jm.2511017","url":null,"abstract":"Sarcopenia is an age-related condition marked by a reduction in muscle mass and strength, and it is associated with impaired muscle regeneration and differentiation. While diseases like cardiovascular and chronic liver disease can induce sarcopenia, there is limited evidence regarding the specific diseases and mechanisms responsible for its development. In skeletal muscle, the loss of muscle mass is accompanied by a decrease in myofilament proteins and the inhibition of muscle differentiation in satellite cells. Bioactive compounds obtained from natural products have been traditionally used as therapeutics for diverse conditions. In this report, we investigated the effect of cinchonidine (CD) extracted from Cinchona tree on muscle differentiation of mouse satellite cells, and myoblast cell lines. CD significantly inhibited muscle differentiation by suppressing myotube formation and gene expression of myogenesis markers. In addition, CD reduced muscle differentiation by blocking phosphorylation of insulin receptor substrate 1 (IRS-1) during insulin-induced signal transduction. Therefore, the results show that CD, an antimalarial agent, inhibited muscle differentiation through the suppression of IRS-1 phosphorylation, suggesting that sarcopenia can be induced by CD.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 12","pages":"e2511017"},"PeriodicalIF":3.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genome analysis of enterohemorrhagic Escherichia coli ATCC 43894 and its pO157-cured strain 277. 肠出血性大肠杆菌ATCC 43894及其po157治愈菌株277的比较基因组分析。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-12-01 Epub Date: 2025-12-31 DOI: 10.71150/jm.2511015

Se Kye Kim, Yong-Joon Cho, Carolyn J Hovde, Sunwoo Hwang, Jonghyun Kim, Jang Won Yoon

{"title":"Comparative genome analysis of enterohemorrhagic Escherichia coli ATCC 43894 and its pO157-cured strain 277.","authors":"Se Kye Kim, Yong-Joon Cho, Carolyn J Hovde, Sunwoo Hwang, Jonghyun Kim, Jang Won Yoon","doi":"10.71150/jm.2511015","DOIUrl":"10.71150/jm.2511015","url":null,"abstract":"Enterohemorrhagic Escherichia coli (EHEC) O157:H7 ATCC 43894 (also known as EDL932) has been widely used as a reference strain for studying the pathophysiology of EHEC. To elucidate the role of a large virulence plasmid pO157 and its relationship with acid resistance, for example, both EHEC ATCC 43894 and its pO157-cured derivative strain 277 were well studied. However, it is unclear whether or not these two strains are isogenic and share the same genetic background. To address this question, we analyzed the whole genome sequences of ATCC 43894 and 277. As expected, three and two closed contigs were identified from ATCC 43894 and 277, respectively; two contigs shared in both strains were a chromosome and a small un-identified plasmid, and one contig found only in ATCC 43894 was pO157. Surprisingly, our pan-genome analyses of the two sequences revealed several genetic variations including frameshift, substitution, and deletion mutations. In particular, the deletion mutation of hdeD and gadE in ATCC 43894 was identified, and further PCR analysis also confirmed their deletion of a 2.5-kb fragment harboring hdeD, gadE, and mdtE in ATCC 43894. Taken together, our findings demonstrate that EHEC ATCC 43894 harbors genetic mutations affecting glutamate-dependent acid resistance system and imply that the pO157-cured EHEC 277 may not be isogenic to ATCC 43894. This is the first report that such genetic differences between both reference strains of EHEC should be considered in future studies on pathogenic E. coli.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 12","pages":"e2511015"},"PeriodicalIF":3.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative perspectives on glycosylation networks in fungi and oomycetes. 真菌和卵菌糖基化网络的综合研究。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-12-01 Epub Date: 2025-12-31 DOI: 10.71150/jm.2510003

Heeji Moon, Hokyoung Son

{"title":"Integrative perspectives on glycosylation networks in fungi and oomycetes.","authors":"Heeji Moon, Hokyoung Son","doi":"10.71150/jm.2510003","DOIUrl":"10.71150/jm.2510003","url":null,"abstract":"Pathogenic fungi pose major threats to both global food security and human health, yet the molecular basis of their virulence remains only partially understood. Beyond genetic and transcriptional control, emerging evidence highlights protein glycosylation as a key post-translational modification that governs fungal development, stress adaptation, and host interactions. Glycosylation regulates protein folding, stability, trafficking, and immune evasion, thereby shaping infection processes across diverse pathogens. While extensively studied in model organisms, our understanding of glycosylation in pathogenic fungi remains fragmented and lacks a coherent framework linking glycosylation dynamics to fungal development and pathogenicity. This review synthesizes recent advances from proteomic, transcriptomic, and glycomic studies in pathogenic fungi, focusing on interspecific variation in glycogenes and enzymes, hierarchical regulatory networks, and glycoprotein-mediated mechanisms of virulence. Finally, we outline current challenges and highlight glycosylation-targeted strategies as promising avenues for antifungal intervention.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 12","pages":"e2510003"},"PeriodicalIF":3.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mucilaginibacter florum sp. nov., isolated from the flower of Coreopsis grandiflora and Mucilaginibacter oryzagri sp. nov., isolated from rice paddy soil in Korea. 从桔梗花中分离的花胶杆菌和从韩国水稻土壤中分离的稻谷胶杆菌。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-12-01 Epub Date: 2025-12-31 DOI: 10.71150/jm.2509014

Parthiban Subramanian, Jun Heo, Daseul Lee, Seunghwan Kim, Hyorim Choi, Yunhee Choi, Yiseul Kim

{"title":"Mucilaginibacter florum sp. nov., isolated from the flower of Coreopsis grandiflora and Mucilaginibacter oryzagri sp. nov., isolated from rice paddy soil in Korea.","authors":"Parthiban Subramanian, Jun Heo, Daseul Lee, Seunghwan Kim, Hyorim Choi, Yunhee Choi, Yiseul Kim","doi":"10.71150/jm.2509014","DOIUrl":"10.71150/jm.2509014","url":null,"abstract":"Two aerobic, Gram-stain-negative, non-motile and rod-shaped bacterial strains designated GGG-R5T and M4-18T were isolated from flowers of golden wave (Coreopsis grandiflora) and rice paddy soil, respectively in the Republic of Korea. Both strains were pigmented and produced flexirubin-type pigments. Based on phylogenetic analysis using 16S rRNA gene sequence, both strains were placed within the genus Mucilaginibacter with M. agri R11T and M. jinjuensis YC7004T both being the closest relatives to GGG-R5T (97.7%) and in case of M4-18T, M. ginsenosidivorax KHI28T (98.5%) was the nearest neighbor. Characteristic to genus Mucilaginibacter, the major cellular fatty acids in both strains were iso-C15:0, iso-C17:0 3-OH, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c); menaquinone-7 was the major menaquinone and phosphatidylethanolamine was the major polar lipid observed. Comparison of genome sequences with the other members of Mucilaginibacter indicated orthologous average nucleotide identity (orthoANI) at 73.3-73.5% for GGG-R5T and 78.9-88.5% for M4-18T. Digital DNA-DNA hybridization (dDDH) values ranged at 19.1-19.7% between GGG-R5T and its neighbor species. In case of M4-18T, the observed range was at 21.9-36.6%. Considering the 16S rRNA similarity, orthoANI and dDDH values as well as comparison of phenotypic and chemotaxonomic characteristics indicated that both strains belonged to genus Mucilaginibacter but were distinctly distinguishable from previously described species. The strains GGG-R5T and M4-18T, therefore represent distinct novel species for which names Mucilaginibacter florum GGG-R5T and Mucilaginibacter oryzagri M4-18T are proposed. The type strains are GGG-R5T (= KACC 22063T = JCM 36590T) and M4-18T (= KACC 22773T = JCM 35894T).","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 12","pages":"e2509014"},"PeriodicalIF":3.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteolytic enzymes from Bacillus subtilis AB2 as antibiofilm adjuvants: Bioprocess optimization, mechanistic insights, and synergy with antibiotics. 枯草芽孢杆菌AB2蛋白水解酶作为抗生素膜佐剂：生物过程优化、机制洞察以及与抗生素的协同作用。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-12-01 Epub Date: 2025-12-31 DOI: 10.71150/jm.2509019

Afra M Baghdadi

{"title":"Proteolytic enzymes from Bacillus subtilis AB2 as antibiofilm adjuvants: Bioprocess optimization, mechanistic insights, and synergy with antibiotics.","authors":"Afra M Baghdadi","doi":"10.71150/jm.2509019","DOIUrl":"10.71150/jm.2509019","url":null,"abstract":"Collagenase and keratinase are two important proteolytic enzymes with recognized applications in biotechnology and medicine, particularly in the enzymatic removal of necrotic tissue and the control of infection. In the present work, a soil isolate of Bacillus subtilis strain AB2 (PX453297.1) was optimized for enzyme production under different nutritional and physicochemical conditions. The enzymes were recovered by ammonium sulphate precipitation and dialysis, examined by SDS-PAGE and zymography, and further assessed for pH and temperature optima, stability, the influence of metal ions, and kinetic parameters. Maximum collagenase activity (4.41 ± 0.22 U/ml) was observed at 37°C and pH 7.5 in a glucose-peptone medium, whereas keratinase production was enhanced between 37 and 40°C at pH 7.5 in lactose-peptone medium. Protein bands of approximately 55 and 33 kDa were detected, representing 6.2- and 5.5-fold purification. Collagenase showed an alkaline optimum (pH 10.0, 37-45°C) with Km 0.31% and Vmax 1.92 U/ml, while keratinase exhibited dual optima (pH 3.0 and ~7.0) with Km 0.27% and Vmax 0.84 U/ml. Biofilm assays revealed that collagenase reduced pre-formed biomass by 62-68% and viable counts by 1.1-1.7 log₁₀, clearly outperforming keratinase (41-57%, 0.7-1.2 log₁₀). When combined with conventional antibiotics, both enzymes potentiated activity, with notable synergy between collagenase and oxacillin against Staphylococcus aureus (FICI 0.31-0.37), ciprofloxacin against Pseudomonas aeruginosa (FICI 0.37-0.50), and meropenem against Klebsiella pneumoniae (FICI 0.28-0.44). These results indicate that B. subtilis AB2 produces collagenase and keratinase with distinct biochemical characteristics and strong antibiofilm properties, underscoring their promise as adjuncts in chronic wound care as well as in industrial applications.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 12","pages":"e2509019"},"PeriodicalIF":3.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omic profiling reveals the impact of keratinase kerZJ on mouse gut homeostasis. 多组学分析揭示了角化酶kerZJ对小鼠肠道稳态的影响。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-12-01 Epub Date: 2025-12-31 DOI: 10.71150/jm.2509011

Xueqing Gan, Yijiao Wen, Si Chen, Famin Ke, Siyuan Liu, Zening Wang, Chunhua Zhang, Xuanting Wang, Qin Wang, Xiaowei Gao

{"title":"Multi-omic profiling reveals the impact of keratinase kerZJ on mouse gut homeostasis.","authors":"Xueqing Gan, Yijiao Wen, Si Chen, Famin Ke, Siyuan Liu, Zening Wang, Chunhua Zhang, Xuanting Wang, Qin Wang, Xiaowei Gao","doi":"10.71150/jm.2509011","DOIUrl":"10.71150/jm.2509011","url":null,"abstract":"Keratinase kerZJ is a multifunctional protease with potential as a feed additive and functional ingredient. Here we performed an integrated multi‑omics evaluation of its biosafety and impact on gut homeostasis in mice. Our findings confirm that kerZJ is well-tolerated, with no evidence of systemic toxicity or intestinal epithelial damage. Integrated transcriptomic and proteomic analyses revealed that kerZJ reinforces intestinal barrier integrity by upregulating extracellular matrix components, including collagen IV, and modulates mucosal immunity by enhancing B-cell activation and antimicrobial peptide defenses without inducing inflammation. Furthermore, kerZJ administration led to a significant upregulation of digestive enzymes and a dose-dependent increase in short-chain fatty acids production. Microbiome analysis showed that while high-dose kerZJ altered community composition, it enriched for beneficial taxa like Lactobacillaceae and did not induce dysbiosis. These results demonstrate that kerZJ safely enhances gut barrier function, promotes a favorable immune and metabolic environment, and fosters a resilient gut ecosystem, supporting its development as a safe feed additive and nutraceutical component.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 12","pages":"e2509011"},"PeriodicalIF":3.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety evaluation and hypolipidemic ability of water-soluble blue pigment extracted by HPD-400 resin from Quambalaria cyanescens. HPD-400树脂提取的金凤莲水溶性蓝色素的安全性评价及降血脂能力。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-11-01 Epub Date: 2025-11-30 DOI: 10.71150/jm.2412011

Ruobing Shi, Chengzhong Wang, Nianping Xue, Zhiguo Zhang

{"title":"Safety evaluation and hypolipidemic ability of water-soluble blue pigment extracted by HPD-400 resin from Quambalaria cyanescens.","authors":"Ruobing Shi, Chengzhong Wang, Nianping Xue, Zhiguo Zhang","doi":"10.71150/jm.2412011","DOIUrl":"10.71150/jm.2412011","url":null,"abstract":"The oral administration of synthetic drugs can effectively reduce blood lipid levels, but adverse reactions may occur. Because of this, the hypolipidemic ability of natural products has been increasingly investigated. We evaluate the safety and hypolipidemic characteristics of a water-soluble blue pigment extracted using HPD-400 resin from the fungus Quambalaria cyanescens. Hypolipidemic ability was examined by constructing a hyperlipidemia model with different doses of blue pigment (50, 100, and 200 mg/kg. mouse body weight) for 28 d. Blue pigment purity increased from 20.32% to 70.70% following treatment with HPD-400 resin. Acute toxicity tests revealed blue pigment sourced from Q. cyanescens to have no toxic effects on mouse body weight, mortality, or behavioral characteristics. Subacute toxicity tests revealed no significant differences in food intake, body weight, or organ weights between treatment groups and controls. Histopathological examination of the liver and kidney tissues of mice administered blue pigment were normal, and serum enzyme activities and blood constituents were also within normal ranges. Blue pigment can significantly reduce the weight of mice, reduce liver and kidney damage and fat accumulation. It can also reduce total cholesterol, triglyceride and low density lipoprotein cholesterol in serum and liver tissue, and increase the level of high density lipoprotein cholesterol. Reduce the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, urea and uric acid in serum. Increase the activities of total superoxide dismutase, glutathione peroxidase and catalase in serum and liver tissue, reduce the content of malondialdehyde, and up-regulate liver lipase and lipoprotein lipase. Our work proves that blue pigment is nontoxic, has the function of reducing blood lipid, and can alleviate obesity-related symptoms by regulating lipid metabolism and oxidative stress.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 11","pages":"e2412011"},"PeriodicalIF":3.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145634848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite-mediated mechanisms linking the urinary microbiome to bladder cancer. 尿微生物群与膀胱癌相关的代谢物介导机制。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2025-11-01 Epub Date: 2025-11-30 DOI: 10.71150/jm.2509001

Thu Anh Trần, Ho Young Lee, Hae Woong Choi

{"title":"Metabolite-mediated mechanisms linking the urinary microbiome to bladder cancer.","authors":"Thu Anh Trần, Ho Young Lee, Hae Woong Choi","doi":"10.71150/jm.2509001","DOIUrl":"10.71150/jm.2509001","url":null,"abstract":"Bladder cancer is the most common malignancy of the urinary tract and is a major health burden globally. Recent advances in microbiome research have revealed that the urinary tract harbors a resident microbial community, overturning the long-held belief in its sterility. Increasing evidence suggests that microbial dysbiosis and microbially derived metabolites contribute to bladder cancer carcinogenesis, progression, and therapeutic responses. Distinct microbial signatures have been observed in bladder cancer patients, with notable differences across disease stages and between primary and recurrent cases. Mechanistic studies have demonstrated that microbe-associated metabolites and toxins can drive DNA damage, chronic inflammation, extracellular matrix remodeling, and epithelial-mesenchymal transition. In addition, biofilm formation allows bacteria to evade immune responses and promotes persistent inflammation, creating a tumor-permissive niche. Beyond pathogenesis, microbial activity also influences therapeutic outcomes; for instance, some microbial pathways can inactivate frontline chemotherapy, while others generate metabolites with anti-tumor properties. Collectively, these patterns define a microbiota-metabolite-immunity axis, presenting opportunities for precision oncology. Targeting microbial pathways, profiling urinary microbiota, and harnessing beneficial metabolites offer promising advancements in biomarker discovery, prognostic refinement, and the development of novel therapeutic strategies for bladder cancer.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"63 11","pages":"e2509001"},"PeriodicalIF":3.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145634693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0