Congregibacter variabilis sp.和 Congregibacter brevis sp.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY
Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-18 DOI:10.1007/s12275-024-00158-5
Hyeonsu Tak, Miri S Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho
{"title":"Congregibacter variabilis sp.和 Congregibacter brevis sp.","authors":"Hyeonsu Tak, Miri S Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho","doi":"10.1007/s12275-024-00158-5","DOIUrl":null,"url":null,"abstract":"<p><p>Two Gram-stain-negative, aerobic, motile by means of flagella, short rod-shaped bacterial strains, designated IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup>, were isolated from coastal seawater samples collected from the South Sea of Korea. Strains IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup> shared 98.6% 16S rRNA gene sequence similarity and were closely related to Congregibacter litoralis KT71<sup>T</sup> (98.8% and 98.7%, respectively). Complete whole-genome sequences of IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup> were 3.93 and 3.86 Mb in size with DNA G + C contents of 54.8% and 54.2%, respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains were 74.5% and 23.4%, respectively, revealing that they are independent species. The two strains showed ANI values of ≤ 75.8% and dDDH values of ≤ 23.0% to the type and only species of the genus Congregibacter (C. litoralis), indicating that each strain represents a novel species. Both strains contained summed feature 3 (comprising C<sub>16:1</sub> ω6c and/or C<sub>16:1</sub> ω7c) and summed feature 8 (comprising C<sub>18:1</sub> ω6c and/or C<sub>18:1</sub> ω7c) as major fatty acid constituents. The predominant isoprenoid quinone detected in both strains was ubiquinone-8 (Q-8). The major polar lipids of the two strains were phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and aminolipids. Based on the phylogenetic, genomic, and phenotypic characterization, strains IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup> were considered to represent two novel species within the genus Congregibacter, for which the names Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. are proposed with IMCC43200<sup>T</sup> (= KCTC 8133<sup>T</sup> = NBRC 116295<sup>T</sup> = CCTCC AB 2023139<sup>T</sup>) and IMCC45268<sup>T</sup> (= KCTC 92921<sup>T</sup> = NBRC 116135<sup>T</sup>) as the type strains, respectively.</p>","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":" ","pages":"739-748"},"PeriodicalIF":3.3000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter.\",\"authors\":\"Hyeonsu Tak, Miri S Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho\",\"doi\":\"10.1007/s12275-024-00158-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Two Gram-stain-negative, aerobic, motile by means of flagella, short rod-shaped bacterial strains, designated IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup>, were isolated from coastal seawater samples collected from the South Sea of Korea. Strains IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup> shared 98.6% 16S rRNA gene sequence similarity and were closely related to Congregibacter litoralis KT71<sup>T</sup> (98.8% and 98.7%, respectively). Complete whole-genome sequences of IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup> were 3.93 and 3.86 Mb in size with DNA G + C contents of 54.8% and 54.2%, respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains were 74.5% and 23.4%, respectively, revealing that they are independent species. The two strains showed ANI values of ≤ 75.8% and dDDH values of ≤ 23.0% to the type and only species of the genus Congregibacter (C. litoralis), indicating that each strain represents a novel species. Both strains contained summed feature 3 (comprising C<sub>16:1</sub> ω6c and/or C<sub>16:1</sub> ω7c) and summed feature 8 (comprising C<sub>18:1</sub> ω6c and/or C<sub>18:1</sub> ω7c) as major fatty acid constituents. The predominant isoprenoid quinone detected in both strains was ubiquinone-8 (Q-8). The major polar lipids of the two strains were phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and aminolipids. Based on the phylogenetic, genomic, and phenotypic characterization, strains IMCC43200<sup>T</sup> and IMCC45268<sup>T</sup> were considered to represent two novel species within the genus Congregibacter, for which the names Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. are proposed with IMCC43200<sup>T</sup> (= KCTC 8133<sup>T</sup> = NBRC 116295<sup>T</sup> = CCTCC AB 2023139<sup>T</sup>) and IMCC45268<sup>T</sup> (= KCTC 92921<sup>T</sup> = NBRC 116135<sup>T</sup>) as the type strains, respectively.</p>\",\"PeriodicalId\":16546,\"journal\":{\"name\":\"Journal of Microbiology\",\"volume\":\" \",\"pages\":\"739-748\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12275-024-00158-5\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12275-024-00158-5","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/18 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

从韩国南海沿岸海水样本中分离出两株革兰氏染色阴性、需氧、通过鞭毛运动的短杆状细菌,命名为 IMCC43200T 和 IMCC45268T。菌株 IMCC43200T 和 IMCC45268T 的 16S rRNA 基因序列相似度为 98.6%,与 litoralis Congregibacter KT71T 关系密切(分别为 98.8% 和 98.7%)。IMCC43200T 和 IMCC45268T 的完整全基因组序列大小分别为 3.93 和 3.86 Mb,DNA G + C 含量分别为 54.8% 和 54.2%。两株菌株之间的平均核苷酸同一性(ANI)和数字DNA-DNA杂交(dDDH)值分别为74.5%和23.4%,表明它们是独立的物种。两株菌株的 ANI 值≤ 75.8%,dDDH 值≤ 23.0%,与同源菌属的模式种和唯一种(C. litoralis)相似,表明每株菌株都代表一个新物种。两株菌株都含有总特征 3(包括 C16:1 ω6c 和/或 C16:1 ω7c)和总特征 8(包括 C18:1 ω6c 和/或 C18:1 ω7c)作为主要脂肪酸成分。在这两种菌株中检测到的最主要的异戊二烯醌是泛醌-8(Q-8)。两株菌株的主要极性脂质为磷脂酰乙醇胺、磷脂酰甘油、磷脂和氨基脂质。根据系统发育、基因组和表型特征,IMCC43200T 和 IMCC45268T 菌株被认为是 Congregibacter 属中的两个新种,分别命名为 Congregibacter variabilis sp.nov.和 Congregibacter brevis sp.nov.,分别以 IMCC43200T(= KCTC 8133T = NBRC 116295T = CCTCC AB 2023139T)和 IMCC45268T(= KCTC 92921T = NBRC 116135T)为模式菌株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter.

Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter.

Two Gram-stain-negative, aerobic, motile by means of flagella, short rod-shaped bacterial strains, designated IMCC43200T and IMCC45268T, were isolated from coastal seawater samples collected from the South Sea of Korea. Strains IMCC43200T and IMCC45268T shared 98.6% 16S rRNA gene sequence similarity and were closely related to Congregibacter litoralis KT71T (98.8% and 98.7%, respectively). Complete whole-genome sequences of IMCC43200T and IMCC45268T were 3.93 and 3.86 Mb in size with DNA G + C contents of 54.8% and 54.2%, respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains were 74.5% and 23.4%, respectively, revealing that they are independent species. The two strains showed ANI values of ≤ 75.8% and dDDH values of ≤ 23.0% to the type and only species of the genus Congregibacter (C. litoralis), indicating that each strain represents a novel species. Both strains contained summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c) and summed feature 8 (comprising C18:1 ω6c and/or C18:1 ω7c) as major fatty acid constituents. The predominant isoprenoid quinone detected in both strains was ubiquinone-8 (Q-8). The major polar lipids of the two strains were phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and aminolipids. Based on the phylogenetic, genomic, and phenotypic characterization, strains IMCC43200T and IMCC45268T were considered to represent two novel species within the genus Congregibacter, for which the names Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. are proposed with IMCC43200T (= KCTC 8133T = NBRC 116295T = CCTCC AB 2023139T) and IMCC45268T (= KCTC 92921T = NBRC 116135T) as the type strains, respectively.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信