Infection Dynamics of Dengue Virus in Caco-2 Cells Depending on Its Differentiation Status.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY
Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-08-30 DOI:10.1007/s12275-024-00161-w
Jayoung Nam, Jisu Lee, Geon A Kim, Seung-Min Yoo, Changhoon Park, Myung-Shin Lee
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引用次数: 0

Abstract

Dengue virus (DENV), from the Flaviviridae family, is the causative agent of dengue fever and poses a significant global health challenge. The virus primarily affects the vascular system and liver; however, a growing body of evidence suggests its involvement in the gastrointestinal (GI) tract, contributing to clinical symptoms such as abdominal pain, vomiting, and diarrhea. However, the mechanisms underlying DENV infection in the digestive system remain largely unexplored. Prior research has detected viral RNA in the GI tissue of infected animals; however, whether the dengue virus can directly infect human enterocytes remains unclear. In this study, we examine the infectivity of human intestinal cell lines to the dengue virus and their subsequent response. We report that the Caco-2 cell line, a model of human enterocytes, is susceptible to infection and capable of producing viruses. Notably, differentiated Caco-2 cells exhibited a lower infection rate yet a higher level of virus production than their undifferentiated counterparts. These findings suggest that human intestinal cells are a viable target for the dengue virus, potentially elucidating the GI symptoms observed in dengue fever and offering a new perspective on the pathogenetic mechanisms of the virus.

Abstract Image

登革病毒在 Caco-2 细胞中的感染动态取决于其分化状态。
登革热病毒(DENV)来自黄热病病毒科,是登革热的病原体,对全球健康构成重大挑战。该病毒主要影响血管系统和肝脏;然而,越来越多的证据表明,它也会影响胃肠道,导致腹痛、呕吐和腹泻等临床症状。然而,消化系统感染 DENV 的机制在很大程度上仍未得到探索。先前的研究在受感染动物的消化道组织中检测到了病毒 RNA,但登革热病毒能否直接感染人类肠细胞仍不清楚。在本研究中,我们研究了人类肠道细胞系对登革病毒的感染性及其随后的反应。我们发现,作为人类肠细胞模型的 Caco-2 细胞系易受感染并能产生病毒。值得注意的是,与未分化的细胞相比,分化的 Caco-2 细胞感染率较低,但产生病毒的水平较高。这些研究结果表明,人类肠道细胞是登革热病毒的一个可行靶标,有可能解释登革热的消化道症状,并为病毒的致病机制提供一个新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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