Journal of Microbiology最新文献_第6页

In Silico Intensive Analysis for the E4 Gene Evolution of Human Adenovirus Species D 人类腺病毒 D 种 E4 基因进化的硅学强化分析

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-30 DOI: 10.1007/s12275-024-00132-1

Chanhee Lee, Anyeseu Park, Jeong Yoon Lee

{"title":"In Silico Intensive Analysis for the E4 Gene Evolution of Human Adenovirus Species D","authors":"Chanhee Lee, Anyeseu Park, Jeong Yoon Lee","doi":"10.1007/s12275-024-00132-1","DOIUrl":"https://doi.org/10.1007/s12275-024-00132-1","url":null,"abstract":"Adenovirus (Ad) is a ubiquitous pathogen capable of infecting a wide range of animals and humans. Human Adenovirus (HAdV) can cause severe infection, particularly in individuals with compromised immune systems. To date, over 110 types of HAdV have been classified into seven species from A to G, with the majority belonging to the human adenovirus species D (HAdV-D). In the HAdV-D, the most significant factor for the creation of new adenovirus types is homologous recombination between viral genes involved in determining the virus tropism or evading immune system of host cells. The E4 gene, consisting of seven Open Reading Frames (ORFs), plays a role in both the regulation of host cell metabolism and the replication of viral genes. Despite long-term studies, the function of each ORF remains unclear. Based on our updated information, ORF2, ORF3, and ORF4 have been identified as regions with relatively high mutations compared to other ORFs in the E4 gene, through the use of in silico comparative analysis. Additionally, we managed to visualize high mutation sections, previously undetectable at the DNA level, through a powerful amino acid sequence analysis tool known as proteotyping. Our research has revealed the involvement of the E4 gene in the evolution of human adenovirus, and has established accurate sequence information of the E4 gene, laying the groundwork for further research.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140831696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Light and Dark Conditions on the Transcriptome of Aging Cultures of Candidatus Puniceispirillum marinum IMCC1322. 光照和黑暗条件对海洋旁氏拟杆菌（Candidatus Puniceispirillum marinum）IMCC1322 老化培养物转录组的影响。

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-25 DOI: 10.1007/s12275-024-00125-0

Ji Hyen Lee, Hyun-Myung Oh

引用次数: 0

Understanding the Diversity and Roles of the Ruminal Microbiome. 了解反刍微生物群的多样性和作用。

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-25 DOI: 10.1007/s12275-024-00121-4

Gi Beom Keum, Sriniwas Pandey, Eun Sol Kim, Hyunok Doo, Jinok Kwak, S. Ryu, Yejin Choi, Juyoun Kang, Sheena Kim, Hyeun Bum Kim

引用次数: 0

Vaccine Development for Severe Fever with Thrombocytopenia Syndrome Virus in Dogs 针对犬严重发热伴血小板减少综合征病毒的疫苗开发

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-18 DOI: 10.1007/s12275-024-00119-y

Seok-Chan Park, Da-Eun Jeong, Sun-Woo Han, Joon-Seok Chae, Joo-Yong Lee, Hyun-Sook Kim, Bumseok Kim, Jun-Gu Kang

{"title":"Vaccine Development for Severe Fever with Thrombocytopenia Syndrome Virus in Dogs","authors":"Seok-Chan Park, Da-Eun Jeong, Sun-Woo Han, Joon-Seok Chae, Joo-Yong Lee, Hyun-Sook Kim, Bumseok Kim, Jun-Gu Kang","doi":"10.1007/s12275-024-00119-y","DOIUrl":"https://doi.org/10.1007/s12275-024-00119-y","url":null,"abstract":"Severe fever with thrombocytopenia syndrome (SFTS) is a life-threatening viral zoonosis. The causative agent of this disease is the Dabie bandavirus, which is usually known as the SFTS virus (SFTSV). Although the role of vertebrates in SFTSV transmission to humans remains uncertain, some reports have suggested that dogs could potentially transmit SFTSV to humans. Consequently, preventive measures against SFTSV in dogs are urgently needed. In the present study, dogs were immunized three times at two-week intervals with formaldehyde-inactivated SFTSV with two types of adjuvants. SFTSV (KCD46) was injected into all dogs two weeks after the final immunization. Control dogs showed viremia from 2 to 4 days post infection (dpi), and displayed white pulp atrophy in the spleen, along with a high level of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling assay (TUNEL) positive area. However, the inactivated SFTSV vaccine groups exhibited rare pathological changes and significantly reduced TUNEL positive areas in the spleen. Furthermore, SFTSV viral loads were not detected at any of the tested dpi. Our results indicate that both adjuvants can be safely used in combination with an inactivated SFTSV formulation to induce strong neutralizing antibodies. Inactivated SFTSV vaccines effectively prevent pathogenicity and viremia in dogs infected with SFTSV. In conclusion, our study highlighted the potential of inactivated SFTSV vaccination for SFTSV control in dogs.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Microbiome-Based Therapies in Chronic Respiratory Diseases 基于微生物的疗法在慢性呼吸系统疾病中的应用

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-18 DOI: 10.1007/s12275-024-00124-1

Se Hee Lee, Jang Ho Lee, Sei Won Lee

{"title":"Application of Microbiome-Based Therapies in Chronic Respiratory Diseases","authors":"Se Hee Lee, Jang Ho Lee, Sei Won Lee","doi":"10.1007/s12275-024-00124-1","DOIUrl":"https://doi.org/10.1007/s12275-024-00124-1","url":null,"abstract":"The application of microbiome-based therapies in various areas of human disease has recently increased. In chronic respiratory disease, microbiome-based clinical applications are considered compelling options due to the limitations of current treatments. The lung microbiome is ecologically dynamic and affected by various conditions, and dysbiosis is associated with disease severity, exacerbation, and phenotype as well as with chronic respiratory disease endotype. However, it is not easy to directly modulate the lung microbiome. Additionally, studies have shown that chronic respiratory diseases can be improved by modulating gut microbiome and administrating metabolites. Although the composition, diversity, and abundance of the microbiome between the gut and lung are considerably different, modulation of the gut microbiome could improve lung dysbiosis. The gut microbiome influences that of the lung via bacterial-derived components and metabolic degradation products, including short-chain fatty acids. This phenomenon might be associated with the cross-talk between the gut microbiome and lung, called gut-lung axis. There are multiple alternatives to modulate the gut microbiome, such as prebiotics, probiotics, and postbiotics ingestion and fecal material transplantation. Several studies have shown that high-fiber diets, for example, present beneficial effects through the production of short-chain fatty acids. Additionally, genetically modified probiotics to secrete some beneficial molecules might also be utilized to treat chronic respiratory diseases. Further studies on microbial modulation to regulate immunity and potentiate conventional pharmacotherapy will improve microbiome modulation techniques, which will develop as a new therapeutic area in chronic respiratory diseases.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balancing Act of the Intestinal Antimicrobial Proteins on Gut Microbiota and Health 肠道抗微生物蛋白对肠道微生物群和健康的平衡作用

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-17 DOI: 10.1007/s12275-024-00122-3

Ye Eun Ra, Ye-Ji Bang

{"title":"Balancing Act of the Intestinal Antimicrobial Proteins on Gut Microbiota and Health","authors":"Ye Eun Ra, Ye-Ji Bang","doi":"10.1007/s12275-024-00122-3","DOIUrl":"https://doi.org/10.1007/s12275-024-00122-3","url":null,"abstract":"The human gut houses a diverse and dynamic microbiome critical for digestion, metabolism, and immune development, exerting profound effects on human health. However, these microorganisms pose a potential threat by breaching the gut barrier, entering host tissues, and triggering infections, uncontrolled inflammation, and even sepsis. The intestinal epithelial cells form the primary defense, acting as a frontline barrier against microbial invasion. Antimicrobial proteins (AMPs), produced by these cells, serve as innate immune effectors that regulate the gut microbiome by directly killing or inhibiting microbes. Abnormal AMP production, whether insufficient or excessive, can disturb the microbiome equilibrium, contributing to various intestinal diseases. This review delves into the complex interactions between AMPs and the gut microbiota and sheds light on the role of AMPs in governing host-microbiota interactions. We discuss the function and mechanisms of action of AMPs, their regulation by the gut microbiota, microbial evasion strategies, and the consequences of AMP dysregulation in disease. Understanding these complex interactions between AMPs and the gut microbiota is crucial for developing strategies to enhance immune responses and combat infections within the gut microbiota. Ongoing research continues to uncover novel aspects of this intricate relationship, deepening our understanding of the factors shaping gut health. This knowledge has the potential to revolutionize therapeutic interventions, offering enhanced treatments for a wide range of gut-related diseases.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin Deep: The Potential of Microbiome Cosmetics 皮肤深处：微生物组化妆品的潜力

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-16 DOI: 10.1007/s12275-024-00128-x

Ju Hee Han, Hei Sung Kim

引用次数: 0

Metabolic Interaction Between Host and the Gut Microbiota During High-Fat Diet-Induced Colorectal Cancer 高脂饮食诱发结直肠癌期间宿主与肠道微生物群之间的代谢相互作用

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-16 DOI: 10.1007/s12275-024-00123-2

Chaeeun Lee, Seungrin Lee, Woongjae Yoo

{"title":"Metabolic Interaction Between Host and the Gut Microbiota During High-Fat Diet-Induced Colorectal Cancer","authors":"Chaeeun Lee, Seungrin Lee, Woongjae Yoo","doi":"10.1007/s12275-024-00123-2","DOIUrl":"https://doi.org/10.1007/s12275-024-00123-2","url":null,"abstract":"Colorectal cancer (CRC) is the second-highest cause of cancer-associated mortality among both men and women worldwide. One of the risk factors for CRC is obesity, which is correlated with a high-fat diet prevalent in Western dietary habits. The association between an obesogenic high-fat diet and CRC has been established for several decades; however, the mechanisms by which a high-fat diet increases the risk of CRC remain unclear. Recent studies indicate that gut microbiota strongly influence the pathogenesis of both high-fat diet-induced obesity and CRC. The gut microbiota is composed of hundreds of bacterial species, some of which are implicated in CRC. In particular, the expansion of facultative anaerobic Enterobacteriaceae, which is considered a microbial signature of intestinal microbiota functional imbalance (dysbiosis), is associated with both high-fat diet-induced obesity and CRC. Here, we review the interaction between the gut microbiome and its metabolic byproducts in the context of colorectal cancer (CRC) during high-fat diet-induced obesity. In addition, we will cover how a high-fat diet can drive the expansion of genotoxin-producing Escherichia coli by altering intestinal epithelial cell metabolism during gut inflammation conditions.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140569858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Therapeutic Potential of Scorpion-Derived Css54 Peptide Against Candida albicans 探索蝎子提取的 Css54 肽对白色念珠菌的治疗潜力

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-08 DOI: 10.1007/s12275-024-00113-4

Jonggwan Park, Hyeongsun Kim, Da Dam Kang, Yoonkyung Park

{"title":"Exploring the Therapeutic Potential of Scorpion-Derived Css54 Peptide Against Candida albicans","authors":"Jonggwan Park, Hyeongsun Kim, Da Dam Kang, Yoonkyung Park","doi":"10.1007/s12275-024-00113-4","DOIUrl":"https://doi.org/10.1007/s12275-024-00113-4","url":null,"abstract":"Candida albicans (C. albicans) is one of the most common opportunistic fungi worldwide, which is associated with a high mortality rate. Despite treatment, C. albicans remains the leading cause of life-threatening invasive infections. Consequently, antimicrobial peptides (AMPs) are potential alternatives as antifungal agents with excellent antifungal activity. We previously reported that Css54, found in the venom of Centrurodies suffusus suffusus (C. s. suffusus) showed antibacterial activity against zoonotic bacteria. However, the antifungal activity of Css54 has not yet been elucidated. The objective of this study was to identify the antifungal activity of Css54 against C. albicans and analyze its mechanism. Css54 showed high antifungal activity against C. albicans. Css54 also inhibited biofilm formation in fluconazole-resistant fungi. The antifungal mechanism of action of Css54 was investigated using membrane-related assays, including the membrane depolarization assay and analysis of the membrane integrity of C. albicans after treatment with Css54. Css54 induced reactive oxygen species (ROS) production in C. albicans, which affected its antifungal activity. Our results indicate that Css54 causes membrane damage in C. albicans, highlighting its value as a potential therapeutic agent against C. albicans infection.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140569410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A PadR family transcriptional repressor regulates the transcription of chromate efflux transporter in Enterobacter sp. Z1 PadR 家族转录抑制因子调控肠杆菌 Z1 中铬酸盐外排转运体的转录

IF 3 4区生物学

Journal of Microbiology Pub Date : 2024-04-08 DOI: 10.1007/s12275-024-00117-0

Xueqi Huo, Zijie Zhou, Hongliang Liu, Gejiao Wang, Kaixiang Shi

{"title":"A PadR family transcriptional repressor regulates the transcription of chromate efflux transporter in Enterobacter sp. Z1","authors":"Xueqi Huo, Zijie Zhou, Hongliang Liu, Gejiao Wang, Kaixiang Shi","doi":"10.1007/s12275-024-00117-0","DOIUrl":"https://doi.org/10.1007/s12275-024-00117-0","url":null,"abstract":"Chromium is a prevalent toxic heavy metal, and chromate [Cr(VI)] exhibits high mutagenicity and carcinogenicity. The presence of the Cr(VI) efflux protein ChrA has been identified in strains exhibiting resistance to Cr(VI). Nevertheless, certain strains of bacteria that are resistant to Cr(VI) lack the presence of ChrB, a known regulatory factor. Here, a PadR family transcriptional repressor, ChrN, has been identified as a regulator in the response of Enterobacter sp. Z1(CCTCC NO: M 2019147) to Cr(VI). The chrN gene is cotranscribed with the chrA gene, and the transcriptional expression of this operon is induced by Cr(VI). The binding capacity of the ChrN protein to Cr(VI) was demonstrated by both the tryptophan fluorescence assay and Ni-NTA purification assay. The interaction between ChrN and the chrAN operon promoter was validated by reporter gene assay and electrophoretic mobility shift assay. Mutation of the conserved histidine residues His14 and His50 resulted in loss of ChrN binding with the promoter of the chrAN operon. This observation implies that these residues are crucial for establishing a DNA-binding site. These findings demonstrate that ChrN functions as a transcriptional repressor, modulating the cellular response of strain Z1 to Cr(VI) exposure.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140569428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0