Journal of Microbiology最新文献_第6页

ITS1-based profiling of the skin mycobiome in truncal acne reveals altered baseline ecology and heterogeneous doxycycline-associated patterns. 基于its1的躯干痤疮皮肤菌群分析揭示了基线生态的改变和多西环素相关的异质性模式。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-02-01 Epub Date: 2026-02-28 DOI: 10.71150/jm.2512013

Nayan Jin, Woo Jun Sul, Hye Rim Do, Hei Sung Kim

{"title":"ITS1-based profiling of the skin mycobiome in truncal acne reveals altered baseline ecology and heterogeneous doxycycline-associated patterns.","authors":"Nayan Jin, Woo Jun Sul, Hye Rim Do, Hei Sung Kim","doi":"10.71150/jm.2512013","DOIUrl":"10.71150/jm.2512013","url":null,"abstract":"Truncal acne represents a biologically distinct manifestation of acne vulgaris, yet its fungal ecology remains incompletely characterized. Previous work using internal transcribed spacer 2 (ITS2) sequencing suggested that truncal acne is associated with altered fungal richness and Malassezia species composition; however, fungal marker choice may influence ecological inference, particularly in sebaceous skin dominated by Malassezia. In this study, we characterized the truncal skin mycobiome of patients with truncal acne and healthy controls using internal transcribed spacer 1 (ITS1) amplicon sequencing. Skin swabs were collected from the upper back, and fungal communities were analyzed using QIIME 2 with taxonomic assignment against the UNITE v10.0 database. Baseline acne-control differences and doxycycline-associated patterns were evaluated using alpha- and beta-diversity metrics and differential abundance analyses. Doxycycline-associated patterns were assessed using paired, within-patient pre- and post-exposure comparisons. ITS1 profiling demonstrated that truncal acne was associated with altered baseline fungal ecology compared with controls, characterized by reduced alpha diversity and ASV-level differences within Malassezia-dominated communities. Beta-diversity analyses showed substantial overlap between acne and control samples, indicating limited global separation. Following doxycycline exposure, fungal communities remained Malassezia-dominant and did not demonstrate uniform convergence toward control profiles; instead, species- and ASV-level differences were heterogeneous across individuals and exposure durations. Together with prior ITS2-based findings, these results underscore the importance of marker-dependent perspectives when interpreting fungal ecology in sebaceous skin.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 2","pages":"e2512013"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lacticaseibacillus paracasei KBL382 contributes to the immunomodulation in THP-1 cells. 副干酪乳杆菌KBL382参与THP-1细胞的免疫调节。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-02-01 Epub Date: 2026-02-28 DOI: 10.71150/jm.2509016

MinJoong Kim, Min Jung Jo, SungJun Park, Seoung Bum Lee, Sung Jae Jang, Cheonghoon Lee, Woon-Ki Kim, GwangPyo Ko

{"title":"Lacticaseibacillus paracasei KBL382 contributes to the immunomodulation in THP-1 cells.","authors":"MinJoong Kim, Min Jung Jo, SungJun Park, Seoung Bum Lee, Sung Jae Jang, Cheonghoon Lee, Woon-Ki Kim, GwangPyo Ko","doi":"10.71150/jm.2509016","DOIUrl":"10.71150/jm.2509016","url":null,"abstract":"Gut microbiome imbalance can induce inflammatory responses via Toll-like receptor 2 (TLR2) signaling pathways. Lactobacillus spp., popularly applied as probiotics in both humans and animals, have come into the spotlight for their strong immunomodulatory effects. We aimed to evaluate the immunomodulatory potential of live or pasteurized Lacticaseibacillus paracasei (L. paracasei) KBL382, isolated from healthy Korean individuals, in an in vitro monocytic THP-1 cell model. Live L. paracasei KBL382 significantly increased TLR2 and MyD88 expressions and induced IRAK1 expression, irrespective of lipopolysaccharide (LPS) stimulation (p < 0.05). Under LPS stimulation, THP-1 cells treated with live L. paracasei KBL382 showed significantly increased interleukin (IL)-6 and IL-10 levels (p < 0.05). Pasteurized L. paracasei exhibited a decrease in IL-12 levels (p < 0.05). Moreover, live L. paracasei KBL382 also markedly elevated A20 and SOCS1 expressions, the critical negative regulators of inflammation, regardless of LPS stimulation (p < 0.05). The expression of IRAK3, another negative regulator of inflammation, was increased in THP-1 cells with live L. paracasei KBL382 under LPS stimulation (p < 0.05). Our findings demonstrate that L. paracasei KBL382 contributes to the immunomodulation in THP-1 cells by coordinating both positive and negative regulatory signaling. L. paracasei KBL382 could be used as a promising probiotic strain for attenuating chronic inflammation through the gut-immune axis mechanisms.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 2","pages":"e2509016"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary characterization of the skin microbiota in basal cell carcinoma: An exploratory pilot study in Korean patients. 基底细胞癌中皮肤微生物群的初步特征：一项针对韩国患者的探索性初步研究。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-02-01 Epub Date: 2026-02-13 DOI: 10.71150/jm.2511012

Hye Lim Keum, Woo Jun Sul, Suyeon Kim, In-Young Chung, Ara Koh, Hei Sung Kim

{"title":"Preliminary characterization of the skin microbiota in basal cell carcinoma: An exploratory pilot study in Korean patients.","authors":"Hye Lim Keum, Woo Jun Sul, Suyeon Kim, In-Young Chung, Ara Koh, Hei Sung Kim","doi":"10.71150/jm.2511012","DOIUrl":"10.71150/jm.2511012","url":null,"abstract":"Basal cell carcinoma (BCC) is the most common form of skin cancer, with ultraviolet radiation recognized as the primary environmental driver; however, the potential contribution of alterations in the skin microbiota remains incompletely understood, particularly in Asian populations. This exploratory pilot study describes bacterial community patterns in BCC lesions compared with contralateral clinically normal skin in 20 Korean patients. Lesional and contralateral samples were obtained using paired skin swabs and punch biopsies and analyzed by full-length 16S rRNA gene sequencing, with targeted quantitative PCR (qPCR) of the roxP antioxidant gene of Cutibacterium acnes. Given the low-biomass nature of skin samples and the exploratory design, analyses focused on descriptive trends rather than confirmatory inference. Across available samples, C. acnes was the dominant taxon, with a trend toward lower relative abundance in BCC lesions, particularly in biopsy-derived datasets. Microbial evenness appeared higher in lesions than controls. Predictive functional profiling suggested reduced representation of vitamin B6 metabolism pathways in lesions, while qPCR analysis of swab samples showed a trend toward lower roxP/16S rRNA ratios in BCC-associated microbiota. These findings should be interpreted cautiously in light of methodological constraints, including sample heterogeneity, lidocaine exposure prior to biopsy, absence of sequencing-based negative controls, and reliance on predictive functional inference. Overall, this pilot study highlights potential differences in skin bacterial community structure between BCC lesions and contralateral skin in a Korean cohort. Larger, methodologically optimized studies incorporating metagenomic and functional validation will be required to determine whether these microbiota shifts contribute to, or result from, BCC-associated changes in the cutaneous environment.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":" ","pages":"e2511012"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of genes and identification of evolutionary evidence in adeno-associated viruses. 腺相关病毒的基因探索和进化证据鉴定。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-02-01 Epub Date: 2026-02-28 DOI: 10.71150/jm.2511016

Chanhee Lee, Jihong Min, Somin Lim, Anyeseu Park, Seokjin Kwak, Soyeon Hwang, Sooyeon Park, Yong-Suk Jang, Se-Yeoun Cha, Sung-Gook Cho, Jeong Yoon Lee

{"title":"Exploration of genes and identification of evolutionary evidence in adeno-associated viruses.","authors":"Chanhee Lee, Jihong Min, Somin Lim, Anyeseu Park, Seokjin Kwak, Soyeon Hwang, Sooyeon Park, Yong-Suk Jang, Se-Yeoun Cha, Sung-Gook Cho, Jeong Yoon Lee","doi":"10.71150/jm.2511016","DOIUrl":"10.71150/jm.2511016","url":null,"abstract":"Adeno-associated virus (AAV) commonly infects humans and non-human primates, generally inducing mild or even asymptomatic outcomes. AAVs have been shaped and diversified by evolutionary pressures, resulting in the identification of 13 serotypes thus far. Each serotype of AAV exhibits distinct tissue tropisms, targeting various organs, including the lung, central nervous system (CNS), liver, and skeletal muscle, thereby establishing AAVs as widely utilized vectors for therapeutic gene delivery. Bioinformatics analysis of specific viruses enables the inference of evolutionary patterns and offers valuable insights for predicting the emergence of novel viruses. While DNA sequence-based analysis has effectively facilitated the observation of mutation patterns accumulating within specific genes, it often provides limited insight into the actual impact of these mutations on proteins, the fundamental functional units. Utilizing proteotyping, an amino acid sequence-based comparative analysis, we identified hypervariable regions (HVR) within the AAV Cap gene and revealed concentrated evolutionary pressures in serotypes 4, 5, 11, and 12. Furthermore, we found that AAV-5 proteins exhibited considerable amino acid sequence divergence compared to those of other serotypes. Despite divergence, all AAV-5 proteins maintained a noticeable structural similarity to their counterparts in other serotypes. Our findings provide sequence-based insights into the evolutionary processes of AAV, facilitating the efficient identification of novel viruses.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 2","pages":"e2511016"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sphingomonas degradans sp. nov. and Sphingomonas paludis sp. nov., isolated from the Han River and a wetland in South Korea. 从韩国汉江和湿地中分离出的鞘氨单胞菌降解sp. 11和Sphingomonas paludis sp. 11。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-01-01 Epub Date: 2026-01-31 DOI: 10.71150/jm.2510010

Seung-Tae Kim, Miryung Kim, Chang-Jun Cha

{"title":"Sphingomonas degradans sp. nov. and Sphingomonas paludis sp. nov., isolated from the Han River and a wetland in South Korea.","authors":"Seung-Tae Kim, Miryung Kim, Chang-Jun Cha","doi":"10.71150/jm.2510010","DOIUrl":"10.71150/jm.2510010","url":null,"abstract":"Two novel bacterial strains, designated CJ20T and CJ99T, belonging to the genus Sphingomonas, were isolated from the Han River in South Korea and a wetland in South Korea, respectively. Cells of both strains were Gram-stain-negative, aerobic, non-motile and yellow-pigmented. Strains were shown to grow optimally at 30˚C and pH 7 in the absence of NaCl on tryptic soy medium. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CJ20T and CJ99T belonged to the genus Sphingomonas and were most closely related to S. asaccharolytica Y-345T and Sphingomonas koreensis JSS26T with 97.87% and 97.58% 16S rRNA gene sequence similarities, respectively. Average nucleotide identity and digital DNA-DNA hybridization values of strain CJ20T with S. asaccharolytica Y-345T were 74.1% and 15.9%, respectively and those values of strain CJ99T with S. koreensis JSS26T were 73.9% and 15.6%, respectively. Both strains contained ubiquinone (Q-10) as the predominant respiratory quinone. The major polar lipids of strains CJ20T and CJ99T comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, and sphingoglycolipid. The predominant fatty acids of both strains were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C16:0. Based on polyphasic taxonomic analyses, strains CJ20T and CJ99T represent novel species of the genus Sphingomonas, for which names Sphingomonas degradans sp. nov. and Sphingomonas paludis are proposed, respectively. The type strains are CJ20T (= KACC 23909 = JCM 37720) and CJ99T (= KACC 24077 = JCM 37956).","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 1","pages":"e2510010"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13576999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of acid mine drainage on nitrogen-fixing microorganisms in rice root zone soil. 酸性矿井排水对水稻根区土壤固氮微生物的影响。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-01-01 Epub Date: 2026-01-31 DOI: 10.71150/jm.2505004

Shengni Tian, Penghui Zhang, Qin Zhang, Yupeng Chen, Caijuan Sun, Dan Huang, Wenye Zhang, Mingzhu Zhang

{"title":"The impact of acid mine drainage on nitrogen-fixing microorganisms in rice root zone soil.","authors":"Shengni Tian, Penghui Zhang, Qin Zhang, Yupeng Chen, Caijuan Sun, Dan Huang, Wenye Zhang, Mingzhu Zhang","doi":"10.71150/jm.2505004","DOIUrl":"10.71150/jm.2505004","url":null,"abstract":"Acid mine drainage (AMD) poses a serious threat to rice paddy ecosystems, yet its impact on the composition and dynamics of soil nitrogen-fixing microorganisms remains poorly understood. In this study, a pot experiment was conducted using paddy soil collected from a mining area under three pollution treatments, to analyze changes in the structure of the nitrogen-fixing microbial community across different growth stages and treatments. The results showed that AMD irrigation led to soil acidification, sulfate accumulation, and a significant reduction in the diversity of nitrogen-fixing microorganisms in the root zone. Compared to the control, the Shannon index decreased by 11.65-24.79% in contaminated soil. LEfSe analysis indicated that AMD enriched metal-tolerant and sulfate-resistant microbial taxa. Irrigation with clean water was insufficient to fully restore the soil environment. The assembly process of the AMD soil community was governed solely by stochastic processes, indicating structural instability of the community. This study suggests that remediation strategies should prioritize neutralizing acidity and restoring nutrient balance to support the stability and recovery of nitrogen-fixing microorganisms. These findings provide new insight into how AMD disrupts diazotrophic community assembly, with direct implications for paddy soil restoration.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 1","pages":"e2505004"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity, skin disorders, and the microbiota: Unraveling a complex web. 肥胖、皮肤病和微生物群：揭开一个复杂的网络。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-01-01 Epub Date: 2026-01-31 DOI: 10.71150/jm.2508007

Yu Ri Woo, Hei Sung Kim

{"title":"Obesity, skin disorders, and the microbiota: Unraveling a complex web.","authors":"Yu Ri Woo, Hei Sung Kim","doi":"10.71150/jm.2508007","DOIUrl":"10.71150/jm.2508007","url":null,"abstract":"Obesity is increasingly recognized as a systemic pro-inflammatory condition that influences not only metabolic and cardiovascular health but also the development and exacerbation of cutaneous inflammatory diseases. This review examines the interplay between obesity, microbial dysbiosis, and two archetypal inflammatory skin disorders-hidradenitis suppurativa (HS) and psoriasis. We highlight how obesity-induced changes in immune signaling, gut permeability, and microbiota composition-both in the gut and the skin-contribute to cutaneous inflammation. Special emphasis is placed on shared pathways such as the Th17/IL-23 and IL-22 signaling axes, adipokine imbalance, and microbial metabolites like short-chain fatty acids and lipopolysaccharides. The review critically evaluates the current literature, distinguishing preclinical insights from clinical evidence, and underscores the potential of microbiota-targeted therapies and metabolic interventions as adjunctive treatment strategies. By integrating metabolic, immunologic, and microbiome data, we synthesize emerging evidence to better understand the gut-skin-obesity interplay and guide future therapeutic innovations.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 1","pages":"e2508007"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13576994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of tri-cistronic CLDN18.2 CAR-T cells incorporating PD-1/CD28 switch and cyclophilin A for enhanced solid tumor immunotherapy. 结合PD-1/CD28开关和亲环蛋白A的三顺反子CLDN18.2 CAR-T细胞的开发用于增强实体瘤免疫治疗。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-01-01 Epub Date: 2026-01-31 DOI: 10.71150/jm.2510017

Heon Ju Lee, Seo Jin Hwang, Eun Hee Jeong, Mi Hee Chang, Bu Yeon Heo, Jaeyul Kwon, Yoona Noh, Jihoon Nah

{"title":"Development of tri-cistronic CLDN18.2 CAR-T cells incorporating PD-1/CD28 switch and cyclophilin A for enhanced solid tumor immunotherapy.","authors":"Heon Ju Lee, Seo Jin Hwang, Eun Hee Jeong, Mi Hee Chang, Bu Yeon Heo, Jaeyul Kwon, Yoona Noh, Jihoon Nah","doi":"10.71150/jm.2510017","DOIUrl":"10.71150/jm.2510017","url":null,"abstract":"Chimeric antigen receptor (CAR)-T cell therapy holds significant potential for the treatment of solid tumors. However, immune suppression and tumor-specific barriers limit its application. Claudin 18.2 (CLDN18.2), a gastric lineage-specific tight junction protein highly expressed in gastric and pancreatic cancers, is a promising therapeutic target. In this study, we aimed to develop a next-generation tri-cistronic CLDN18.2-directed CAR-T cell platform that integrates a programmed cell death protein 1 (PD-1)/CD28 chimeric switch receptor with cyclophilin A (CypA). This platform sought to counteract PD-1-mediated immunosuppression and enhance T-cell activation and persistence. We generated CLDN18.2 CAR-T cells incorporating costimulatory inducible T-cell costimulator (ICOS) domains using lentiviral vector-based recombinant engineering. We further evaluated their cytokine release, cytotoxic activity, and safety profiles. In vitro, tri-cistronic CAR-T cells exhibited markedly increased interferon γ and tumor necrosis factor α secretion and enhanced cytotoxicity against CLDN18.2-positive gastric cancer cells compared with conventional CAR-T constructs. In vivo, these cells showed superior antitumor efficacy and sustained tumor regression without observable toxicity in xenograft gastric cancer models. Collectively, these findings demonstrate that the integration of PD-1/CD28 signaling and CypA within a tri-cistronic framework significantly reinforces CAR-T cell functionality and durability. This suggests strong clinical potential as a next-generation immunotherapy for solid tumors.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 1","pages":"e2510017"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological characteristics of the truncal skin mycobiome in acne and its association with doxycycline exposure. 痤疮躯干皮肤真菌群落的生态学特征及其与强力霉素暴露的关系。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-01-01 Epub Date: 2026-01-31 DOI: 10.71150/jm.2511019

Hyun Ji Lee, Yong-Joon Cho, Nayan Jin, Piyapat Rintarhat, Won Hee Jung, Hei Sung Kim

{"title":"Ecological characteristics of the truncal skin mycobiome in acne and its association with doxycycline exposure.","authors":"Hyun Ji Lee, Yong-Joon Cho, Nayan Jin, Piyapat Rintarhat, Won Hee Jung, Hei Sung Kim","doi":"10.71150/jm.2511019","DOIUrl":"10.71150/jm.2511019","url":null,"abstract":"Truncal acne significantly impairs quality of life yet remains underexplored relative to facial acne, particularly with respect to fungal ecology. The trunk represents a distinct cutaneous niche characterized by thicker epidermis, larger follicular units, and frequent occlusion, and harbors a high abundance of Malassezia species. In this study, we used internal transcribed spacer 2 (ITS2) amplicon sequencing to characterize the truncal mycobiome in patients with acne and in healthy controls and to compare fungal community features across doxycycline exposure groups. Although serial sampling was planned, seven participants contributed a single follow-up sample after doxycycline treatment, and only two participants contributed multiple follow-up samples sufficient for true within-subject longitudinal analyses; therefore, most analyses represent exposure-stratified cross-sectional comparisons rather than confirmed temporal change. At baseline, truncal acne lesions exhibited increased fungal richness and distinct community composition compared with controls. Acne lesions were more frequently enriched for Malassezia globosa, whereas healthy controls were dominated by M. sympodialis. Across doxycycline exposure groups, fungal communities remained Malassezia-dominant with substantial inter-individual variability. Doxycycline exposure was associated with partial and heterogeneous differences in Malassezia species composition without uniform normalization toward control profiles. Because only fungal sequencing was performed, bacterial-fungal interactions were inferred from prior literature and not directly measured. These findings indicate that truncal acne is associated with a distinct fungal community structure and highlight the need for integrated, longitudinal multi-omics studies to clarify treatment-associated microbial dynamics.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 1","pages":"e2511019"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D disrupts NS1-TUFM interaction to suppress pathogenic mitophagy in RSV-induced mitochondrial injury of bronchial epithelial cells. 维生素D破坏NS1-TUFM相互作用，抑制rsv诱导的支气管上皮细胞线粒体损伤的病原性线粒体自噬。

IF 3.8 4区生物学

Journal of Microbiology Pub Date : 2026-01-01 Epub Date: 2026-01-31 DOI: 10.71150/jm.2508009

Li Peng, Yao Liu, Xiaofang Ding, Tuhong Yang, Lili Zhong, Fangcai Li

{"title":"Vitamin D disrupts NS1-TUFM interaction to suppress pathogenic mitophagy in RSV-induced mitochondrial injury of bronchial epithelial cells.","authors":"Li Peng, Yao Liu, Xiaofang Ding, Tuhong Yang, Lili Zhong, Fangcai Li","doi":"10.71150/jm.2508009","DOIUrl":"10.71150/jm.2508009","url":null,"abstract":"This study aims to examine the mechanism by which vitamin D mitigates bronchiolitis caused by respiratory syncytial virus (RSV) through the regulation of RSV nonstructural protein 1 (NS1)-TUFM-mediated mitophagy in bronchial epithelial cells. Clinical serum and PBMC samples from RSV-infected children and healthy controls were analyzed for vitamin D, mitochondrial DNA, mitophagy markers (LC3, ATG5, VDAC1, TOMM20, and COXIV), TUFM, and inflammatory cytokines (IL-6, IL-8, and TNF-α). In vitro, human bronchial epithelial cells Beas-2B were transfected with RSV-NS1 plasmid and TUFM silencing or overexpression constructs. Vitamin D (0.1-10 μM) was administered to evaluate mitophagy inhibition using Western blot, immunofluorescence, and JC-1 staining. NS1-TUFM interaction was confirmed by co-immunoprecipitation. RSV-positive patients exhibited reduced serum vitamin D, elevated TUFM and mitophagy markers, impaired mitochondrial mass, and increased inflammation. Vitamin D inversely correlated with LC3 and TUFM. RSV-NS1 overexpression induced mitochondrial translocation of NS1, TUFM-dependent mitophagy activation, and mitochondrial dysfunction (JC-1 depolarization). Vitamin D (10 μM) suppressed mitophagy by redistributing NS1 to the cytosol and reducing mitochondrial TUFM. TUFM overexpression abolished the protective effects of vitamin D on mitophagy and inflammation. In conclusion, vitamin D inhibits mitophagy in bronchial epithelial cells infected with RSV by disrupting NS1-TUFM interaction, suggesting that the vitamin D-TUFM axis may serve as a potential therapeutic target.","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"64 1","pages":"e2508009"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0