Journal of microscopy最新文献_第4页

Fast Gaussian fitting peak extraction for confocal microscopy 用于共聚焦显微镜的快速高斯拟合峰提取。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-07-16 DOI: 10.1111/jmi.70012

Jian Liu, Zhenlong Xu, Ziyi Wang, Xiaoyu You, Chenguang Liu

引用次数: 0

Influence of polarisation states on the axial point spread function in subtractive second harmonic generation microscopy with a vortex beam 偏振态对涡旋光束相减二次谐波产生显微镜轴向点扩展函数的影响。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-07-15 DOI: 10.1111/jmi.70013

Huizhen Xu, Kanwarpal Singh

引用次数: 0

Ptychographic analysis of human bone marrow-derived mesenchymal stem cell morphology: The impact of cell senescence. 人骨髓源间充质干细胞形态的病理学分析：细胞衰老的影响。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-11 DOI: 10.1111/jmi.70003

Lorenzo Anconelli, Giovanna Farruggia, Isabella Zafferri, Francesca Borsetti, Stefano Iotti, Francesca Rossi, Jeanette A Maier

{"title":"Ptychographic analysis of human bone marrow-derived mesenchymal stem cell morphology: The impact of cell senescence.","authors":"Lorenzo Anconelli, Giovanna Farruggia, Isabella Zafferri, Francesca Borsetti, Stefano Iotti, Francesca Rossi, Jeanette A Maier","doi":"10.1111/jmi.70003","DOIUrl":"https://doi.org/10.1111/jmi.70003","url":null,"abstract":"Mesenchymal stem cells (MSC) undergo replicative senescence, a state of irreversible cell cycle arrest that limits their utility in regenerative medicine applications. To identify novel markers of senescence useful to enhance the quality of MSC-based therapies, we compared young and senescent human bone marrow-derived mesenchymal stem cells (hMSCs) using a non-invasive, label-free approach based on quantitative phase imaging (QPI) with the Livecyte microscope. Senescent hMSCs demonstrated substantial morphological alterations, including a threefold increase in cell area, elevated dry mass, reduced thickness, and decreased sphericity compared to their younger counterparts. Additionally, motility metrics such as instantaneous velocity and displacement were significantly reduced in senescent cells, underscoring functional impairments that could hinder their therapeutic potential in regenerative medicine. The application of QPI offers a promising tool for monitoring cellular health, identifying, and potentially eliminating, senescent cells to improve the quality and effectiveness of MSC-based therapies.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of reliable and representative DVC measurements for musculoskeletal tissues 可靠和代表性的肌肉骨骼组织DVC测量的概述。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-07-10 DOI: 10.1111/jmi.70008

Gianluca Tozzi, Enrico Dall'Ara

{"title":"An overview of reliable and representative DVC measurements for musculoskeletal tissues","authors":"Gianluca Tozzi, Enrico Dall'Ara","doi":"10.1111/jmi.70008","DOIUrl":"10.1111/jmi.70008","url":null,"abstract":"Musculoskeletal tissues present complex hierarchical structures and mechanical heterogeneity across multiple length scales, making them difficult to characterise accurately. Digital volume correlation (DVC) is a non-destructive imaging technique that enables quantification of internal 3D strain fields under realistic loading conditions, offering a unique tool to investigate the biomechanics of biological tissues and biomaterials. This review highlights recent advancements in DVC, focusing on its applications across scales ranging from organ- to tissue-level mechanics in both mineralised and soft tissues. Instead of a traditional systematic review, we identify key technical challenges including the treatment of tissue interfaces, border effects, and the quantification of uncertainty in DVC outputs. Strategies for improving measurement accuracy and reliability are discussed. We also report on the increasing use of DVC in in vivo applications, its coupling with computational modelling to inform and validate biomechanical simulations, and its recent integration with data-driven methods such as deep learning to directly predict displacement and strain fields. Additionally, we examine its application in tissue engineering and implant–tissue interface assessment. By addressing such areas, we outline current limitations and emerging opportunities for future research. These include advancing precision, enabling clinical translation, and leveraging machine learning to create more robust, automated, and predictive DVC workflows for musculoskeletal health and tissue engineering.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"300 1","pages":"3-17"},"PeriodicalIF":1.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on critique and rebuttal of ‘On the pixel selection criterion for the calculation of the Pearson's correlation coefficient in fluorescence microscopy’ 对《关于荧光显微镜中皮尔逊相关系数计算的像素选择标准》一文的批判和反驳的评论。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-09 DOI: 10.1111/jmi.70006

Fabrice Cordelieres, Michelle Peckham

引用次数: 0

Response to Adler and Parmryd 对Adler和Parmryd的回应。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-09 DOI: 10.1111/jmi.13428

Sergio G. Lopez, Sebastian Samwald, Sally Jones, Christine Faulkner

引用次数: 0

Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy. 体积电子显微镜检测疟原虫结构的冷冻替代方案优化。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-09 DOI: 10.1111/jmi.70007

Rachel Rachid, Camila Wendt, Wanderley de Souza, Kildare Miranda

{"title":"Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy.","authors":"Rachel Rachid, Camila Wendt, Wanderley de Souza, Kildare Miranda","doi":"10.1111/jmi.70007","DOIUrl":"https://doi.org/10.1111/jmi.70007","url":null,"abstract":"Malaria is one of the deadliest infectious diseases in the world, annually responsible for over 400,000 deaths. It is caused by parasites of the genus Plasmodium, which undergo remarkable structural changes during their development within different cells across various hosts. An important approach to understand the structural basis of biochemical and physiological processes during Plasmodium infection has been the quantitative measurement of dimensional parameters obtained by different microscopy techniques. In this regard, sample preparation, particularly electron microscopy protocols that rely on room-temperature chemical fixation, has posed significant challenges, as it is known to produce artefacts such as shrinking, swelling and displacement of structures and osmolytes. In contrast, specimen immobilisation by cryofixation followed by freeze substitution minimises these artefacts and provides better sample preservation. Nevertheless, the composition of the freeze substitution medium may vary depending on the cell type, making it a critical factor for achieving optimal sample preparation. In this work, we optimised a freeze substitution protocol for the structural analysis of intraerythrocytic stages of the murine malaria models Plasmodium chabaudi and P. berghei. We tested different freeze substitution recipes, considering the biochemical composition of malaria membranes, and compared the results with those obtained through conventional chemical fixation. Overall, the results showed a significant improvement on the preservation of cell morphology and haemozoin crystals. Establishing an efficient and reproducible freeze substitution protocol for murine malaria models provides an important tool for advancing our understanding of the structural organisation of Plasmodium spp.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying colocalisation by correlation: Use pixels with both fluorophores within biologically meaningful ROIs 通过相关性量化共定位：在具有生物学意义的roi中使用具有两个荧光团的像素。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-08 DOI: 10.1111/jmi.13429

Jeremy Adler, Ingela Parmryd

引用次数: 0

A workflow for semi-automated volume correlative light microscopy and transmission electron tomography 半自动化体相关光学显微镜和透射电子断层扫描的工作流程。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-06-30 DOI: 10.1111/jmi.13436

Kohki Konishi, Guilherme Neves, Matthew Russell, Masafumi Mimura, Juan Burrone, Roland Fleck

{"title":"A workflow for semi-automated volume correlative light microscopy and transmission electron tomography","authors":"Kohki Konishi, Guilherme Neves, Matthew Russell, Masafumi Mimura, Juan Burrone, Roland Fleck","doi":"10.1111/jmi.13436","DOIUrl":"10.1111/jmi.13436","url":null,"abstract":"Volume Correlative Light and Electron Microscopy (vCLEM) is a powerful method for assessing the ultrastructure of molecularly defined subcellular domains. A central challenge in vCLEM has been the efficient navigation of Regions of Interest (ROIs) across multimodal and multiscale imaging datasets. We developed two key tools to overcome this challenge. First, we developed a multimodal image registration tool (SegReg) that utilizes segmentation of common objects across modalities and uses a Graphical Processing Unit (GPU) for registration of imaging datasets in two and three dimensions. Secondly, we developed a dedicated image viewer to visualize multimodal image registration in three dimensions (NavROI). Here, we demonstrate the integrated use of SegReg and NavROI to navigate large mouse tissue blocks with preserved fluorescent signals to allow selective targeting for TEM tomography of ROIs containing synapses and the cisternal organelle on the proximal region of the axon of a selected pyramidal neuron. By providing real time guidance to precise X-Y trimming of selected ROIs, reliable estimates of cutting depth relative to ROIs and a clear visual navigation of multimodal and multiscale images, our integrated workflow significantly improves the efficiency and accessibility of vCLEM analysis.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"300 1","pages":"94-107"},"PeriodicalIF":1.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DeepEM Playground: Bringing deep learning to electron microscopy labs DeepEM Playground：将深度学习带入电子显微镜实验室。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-06-28 DOI: 10.1111/jmi.70005

Hannah Kniesel, Poonam Poonam, Tristan Payer, Tim Bergner, Pedro Hermosilla, Timo Ropinski

引用次数: 0