Journal of microscopy最新文献_第10页

Towards quantification of doping in gallium arsenide nanostructures by low-energy scanning electron microscopy and conductive atomic force microscopy 利用低能扫描电子显微镜和传导原子力显微镜量化砷化镓纳米结构中的掺杂。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-01-18 DOI: 10.1111/jmi.13263

Ran Guo, Thomas Walther

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The use of fluorescence lifetime imaging (FLIM) for in situ microbial detection in complex mineral substrates 利用荧光寿命成像（FLIM）对复杂矿物基质中的微生物进行原位检测。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-01-17 DOI: 10.1111/jmi.13264

Yekaterina Chmykh, Jay L. Nadeau

{"title":"The use of fluorescence lifetime imaging (FLIM) for in situ microbial detection in complex mineral substrates","authors":"Yekaterina Chmykh, Jay L. Nadeau","doi":"10.1111/jmi.13264","DOIUrl":"10.1111/jmi.13264","url":null,"abstract":"The utility of fluorescence lifetime imaging microscopy (FLIM) for identifying bacteria in complex mineral matrices was investigated. Baseline signals from unlabelled Bacillus subtilis and Euglena gracilis, and Bacillus subtilis labelled with SYTO 9 were obtained using two-photon excitation at 730, 750 and 800 nm, identifying characteristic lifetimes of photosynthetic pigments, unpigmented cellular autofluorescence, and SYTO 9. Labelled and unlabelled B. subtilis were seeded onto marble and gypsum samples containing endolithic photosynthetic cyanobacteria and the ability to distinguish cells from mineral autofluorescence and nonspecific dye staining was examined in parallel with ordinary multichannel confocal imaging. It was found that FLIM enabled discrimination of SYTO 9 labelled cells from background, but that the lifetime of SYTO 9 was shorter in cells on minerals than in pure culture under our conditions. Photosynthetic microorganisms were easily observed using both FLIM and confocal. Unlabelled, nonpigmented bacteria showed weak signals that were difficult to distinguish from background when minerals were present, though cellular autofluorescence consistent with NAD(P)H could be seen in pure cultures, and phasor analysis permitted detection on rocks. Gypsum and marble samples showed similar autofluorescence profiles, with little autofluorescence in the yellow-to-red range. Lifetime or time-gated imaging may prove a useful tool for environmental microbiology.LAY DESCRIPTION: The standard method of bacterial enumeration is to label the cells with a fluorescent dye and count them under high-power fluorescence microscopy. However, this can be difficult when the cells are embedded in soil and rock due to fluorescence from the surrounding minerals and dye binding to ambiguous features of the substrate. The use of fluorescence lifetime imaging (FLIM) can disambiguate these signals and allow for improved detection of bacteria in environmental samples.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Atomic resolution imaging using a novel, compact and stiff scanning tunnelling microscope in cryogen-free superconducting magnet 在无低温超导磁体中进行原子分辨的新颖、紧凑和坚硬的扫描隧穿显微镜。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-01-15 DOI: 10.1111/jmi.13262

Behnam Esmaeilzadeh, Muhammad Touqeer, Liu Junwei, Shaofeng Zheng, Tao Geng, Yubin Hou, Qingyou Lu

{"title":"Atomic resolution imaging using a novel, compact and stiff scanning tunnelling microscope in cryogen-free superconducting magnet","authors":"Behnam Esmaeilzadeh, Muhammad Touqeer, Liu Junwei, Shaofeng Zheng, Tao Geng, Yubin Hou, Qingyou Lu","doi":"10.1111/jmi.13262","DOIUrl":"10.1111/jmi.13262","url":null,"abstract":"We present the design and performance of a novel scanning tunnelling microscope (STM) operating in a cryogen-free superconducting magnet. Our home-built STM head is compact (51.5 mm long and 20 mm in diameter) and has a single arm that provides complete openness in the scanning area between the tip and sample. The STM head consists of two piezoelectric tubes (PTs), a piezoelectric scanning tube (PST) mounted on a well-polished zirconia shaft, and a large PT housed in a sapphire tube called the motor tube. The main body of the STM head is made of tantalum. In this design, we fixed the sapphire tube to the frame with screws so that the tube's position can be changed quickly. To analyse the stiffness of the STM head unit, we identified the lowest eigenfrequencies with 3 and 4 kHz in the bending modes, 8 kHz in a torsional mode, and 9 kHz in a longitudinal mode by finite element analysis, and also measured the low drift rates in the X–Y plane and in the Z direction. The high performance of the home-built STM was demonstrated by images of the hexagonal graphite lattice at 300 K and in a sweeping magnetic field from 0 T to 9 T. Our results confirm the high stability, vibration resistance, insensitivity to high magnetic fields and the application potential of our newly developed STM for the investigation of low-frequency systems with high static support stiffness in physics, chemistry, material and biological sciences.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-01-15 DOI: 10.1111/jmi.13195

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A multiangle polarised imaging-based method for thin section segmentation 基于多角度偏振成像的薄片分割方法。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-01-15 DOI: 10.1111/jmi.13261

Yan Chen, Yu Yi, Yongfang Dai, Xiangchao Shi

{"title":"A multiangle polarised imaging-based method for thin section segmentation","authors":"Yan Chen, Yu Yi, Yongfang Dai, Xiangchao Shi","doi":"10.1111/jmi.13261","DOIUrl":"10.1111/jmi.13261","url":null,"abstract":"The most crucial task of petroleum geology is to explore oil and gas reservoirs in the deep underground. As one of the analysis techniques in petroleum geological research, rock thin section identification method includes particle segmentation, which is one of the key steps. A conventional sandstone thin section image typically contains hundreds of mineral particles with blurred boundaries and complex microstructures inside the particles. Moreover, the complex lithology and low porosity of tight sandstone make traditional image segmentation methods unsuitable for solving the complex thin section segmentation problems. This paper combines petrology and image processing technologies. First, polarised sequence images are aligned, and then the images are transformed to the HSV colour space to extract pores. Second, particles are extracted according to their extinction characteristics. Last, a concavity and corner detection matching method is used to process the extracted particles, thereby completing the segmentation of sandstone thin section images. The experimental results show that our proposed method can more accurately fit the boundaries of mineral particles in sandstone images than existing image segmentation methods. Additionally, when applied in actual production scenarios, our method exhibits excellent performance, greatly improving thin section identification efficiency and significantly assisting experts in identification.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Comparison of holotomographic microscopy and coherence-controlled holographic microscopy 全息显微镜和相干控制全息显微镜的比较。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-01-10 DOI: 10.1111/jmi.13260

Vera Chvalova, Tomas Vomastek, Tomas Grousl

{"title":"Comparison of holotomographic microscopy and coherence-controlled holographic microscopy","authors":"Vera Chvalova, Tomas Vomastek, Tomas Grousl","doi":"10.1111/jmi.13260","DOIUrl":"10.1111/jmi.13260","url":null,"abstract":"Quantitative phase imaging (QPI) is a powerful tool for label-free visualisation of living cells. Here, we compare two QPI microscopes – the Telight Q-Phase microscope and the Nanolive 3D Cell Explorer-fluo microscope. Both systems provide unbiased information about cell morphology, such as individual cell dry mass, perimeter and area. The Q-Phase microscope uses artefact-free, coherence-controlled holographic imaging technology to visualise cells in real time with minimal phototoxicity. The 3D Cell Explorer-fluo employs laser-based holotomography to reconstruct 3D images of living cells, visualising their internal structures and dynamics. Here, we analysed the strengths and limitations of both microscopes when examining two morphologically distinct cell lines – the cuboidal epithelial MDCK cells which form multicellular clusters and solitary growing Rat2 fibroblasts. We focus mainly on the ability of the devices to generate images suitable for single-cell segmentation by the built-in software, and we discuss the segmentation results and quantitative data generated from the segmented images. We show that both microscopes offer slightly different advantages, and the choice between them depends on the specific requirements and goals of the user.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139403306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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IF 2 4区工程技术

Journal of microscopy Pub Date : 2023-12-28 DOI: 10.1111/jmi.13194

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Visualisation of microalgal lipid bodies through electron microscopy 通过电子显微镜观察微藻脂质体。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2023-12-27 DOI: 10.1111/jmi.13259

Ellen Verwee, Davy Van de Walle, Michiel De Bruyne, Esther Mienis, Mirna Sekulic, Peter Chaerle, Wim Vyverman, Imogen Foubert, Koen Dewettinck

{"title":"Visualisation of microalgal lipid bodies through electron microscopy","authors":"Ellen Verwee, Davy Van de Walle, Michiel De Bruyne, Esther Mienis, Mirna Sekulic, Peter Chaerle, Wim Vyverman, Imogen Foubert, Koen Dewettinck","doi":"10.1111/jmi.13259","DOIUrl":"10.1111/jmi.13259","url":null,"abstract":"In this study, transmission electron microscopy (TEM) and cryo-scanning electron microscopy (cryo-SEM) were evaluated for their ability to detect lipid bodies in microalgae. To do so, Phaeodactylum tricornutum and Nannochloropsis oculata cells were harvested in both the mid-exponential and early stationary growth phase. Two different cryo-SEM cutting methods were compared: cryo-planing and freeze-fracturing. The results showed that, despite the longer preparation time, TEM visualisation preceded by cryo-immobilisation allows a clear detection of lipid bodies and is preferable to cryo-SEM. Using freeze-fracturing, lipid bodies were rarely detected. This was only feasible if crystalline layers in the internal structure, most likely related to sterol esters or di-saturated triacylglycerols, were revealed. Furthermore, lipid bodies could not be detected using cryo-planing. Cryo-SEM is also not the preferred technique to recognise other organelles besides lipid bodies, yet it did reveal chloroplasts in both species and filament-containing organelles in cryo-planed Nannochloropsis oculata samples.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Correlative super-resolution microscopy with deep UV reactivation. 相关超分辨率显微镜与深紫外再活化。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2023-12-25 DOI: 10.1111/jmi.13258

Kirti Prakash

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The evolution of indium precipitation in gallium focused ion beam prepared samples of InGaAs/InAlAs quantum wells under electron beam irradiation 镓聚焦离子束制备的 InGaAs/InAlAs 量子阱样品中铟沉淀在电子束辐照下的演变。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2023-12-19 DOI: 10.1111/jmi.13251

Shuo Liu, Jiawei Dong, Zhenyu Ma, Wenyu Hu, Yong Deng, Yuechun Shi, Xiaoyi Wang, Yang Qiu, Thomas Walther

{"title":"The evolution of indium precipitation in gallium focused ion beam prepared samples of InGaAs/InAlAs quantum wells under electron beam irradiation","authors":"Shuo Liu, Jiawei Dong, Zhenyu Ma, Wenyu Hu, Yong Deng, Yuechun Shi, Xiaoyi Wang, Yang Qiu, Thomas Walther","doi":"10.1111/jmi.13251","DOIUrl":"10.1111/jmi.13251","url":null,"abstract":"Gallium ion (Ga+) beam damage induced indium (In) precipitation in indium gallium arsenide (InGaAs)/indium aluminium arsenide (InAlAs) multiple quantum wells and its corresponding evolution under electron beam irradiation was investigated by valence electron energy loss spectroscopy (VEELS) and high-angle annular dark-field imaging (HAADF) in scanning transmission electron microscopy (STEM). Compared with argon ion milling for sample preparation, the heavier projectiles of Ga+ ions pose a risk to trigger In formation in the form of tiny metallic In clusters. These are shown to be sensitive to electron irradiation and can increase in number and size under the electron beam, deteriorating the structure. Our finding reveals the potential risk of formation of In clusters during focused ion beam (FIB) preparation of InGaAs/InAlAs quantum well samples and their subsequent growth under STEM-HAADF imaging, where initially invisible In clusters of a few atoms can move and swell during electron beam exposure.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138802144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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