Journal of microscopy最新文献

{"title":"Remotely accessible optical microscopy education through a wide (and global) lens","authors":"Zachary Sanchez,&nbsp;Miguel de Jesus,&nbsp;Moses David,&nbsp;Ali Taheri,&nbsp;Sunday Yinka Olatunji,&nbsp;Mai Rahmoon,&nbsp;Kevin W. Eliceiri,&nbsp;Anita Mahadevan-Jansen,&nbsp;Bryan A. Millis","doi":"10.1111/jmi.70112","DOIUrl":"10.1111/jmi.70112","url":null,"abstract":"<p>Access to rigorous optical microscopy education remains unevenly distributed across the globe despite widespread use of optical methods. While lower-resourced settings certainly feel this burden, it is far from a foregone conclusion that such opportunities are ubiquitous, even at well-funded institutions. Despite a growing number of online educational resources and other tools, many biomedical researchers learn microscopy in a task-specific manner, and without the conceptual foundation to maximise the potential of its capabilities, robustly interpret data, avoid bias, and/or troubleshoot effectively. Given microscopy's significant impact in the discovery process and status as a cornerstone scientific tool, we developed a remotely accessible, global, and highly interactive program to help address the education gap with respect to the fundamentals of microscopy, irrespective of one's location or ability to access high-end platforms. Here we reflect on the design and evolution of the resulting ‘Widening the Lens’ (WtL) program: what worked, what didn't, and how the course adapted in response, with the intent of enabling others to leverage our experience to develop programs of their own. We also describe our partnership with critical network organisations, including the African BioImaging Consortium (ABIC) to implement a WtL based train-the-trainer model across the African continent. WtL demonstrates that equitable microscopy education is not only achievable, but that closing education gaps involves building a community in concert with likeminded educational opportunities and networks across the globe.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"406-418"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147948301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ancient trading hubs of modern science: Bridging the divide between microscopists and data scientists","authors":"Yasmin M. Kassim,&nbsp;Uri Manor","doi":"10.1111/jmi.70108","DOIUrl":"10.1111/jmi.70108","url":null,"abstract":"<p>Biomedical imaging is increasingly defined by a paradox: advances in microscopy now enable the routine generation of extraordinarily rich, high-dimensional datasets, yet the extraction of reproducible, biologically meaningful insight from those data often remains a major bottleneck. This mismatch has widened a growing divide between microscopists and experimental biologists, who generate complex image data, and data scientists, who develop the computational tools needed to interpret them. In this Perspective, we argue that this divide is not merely technical, but also cultural, shaped by different assumptions about validation, dissemination, workflow design, and what constitutes meaningful success. We highlight how artificial intelligence and computational microscopy are simultaneously overrated when treated as turnkey solutions and underrated when deployed thoughtfully to reshape experimental design, data acquisition, and biological discovery. We further argue that current validation practices often fail to capture biological fidelity, creating a dangerous gap between benchmark performance and real-world utility. Finally, we propose that imaging core facilities should evolve beyond service models into collaborative hubs and training grounds where acquisition, analysis, and interpretation are integrated from the outset. By fostering shared standards, iterative workflows, and cross-disciplinary training, core facilities can help bridge the divide between photons and pixels and enable a more rigorous, scalable, and biologically grounded future for imaging science.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"320-333"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147972968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TOC - Issue Information","authors":"","doi":"10.1111/jmi.70100","DOIUrl":"https://doi.org/10.1111/jmi.70100","url":null,"abstract":"","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"241-242"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148099334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening research infrastructure through local networks of light microscopy facilities","authors":"Ann Wheeler,&nbsp;Dale A Moulding","doi":"10.1111/jmi.70092","DOIUrl":"10.1111/jmi.70092","url":null,"abstract":"<p>Light microscopy core facilities are widely established in research institutions, providing access to advanced instrumentation with expert guidance. Centralising technology and expertise within these facilities improves research output, fosters collaboration between research groups and reduces costs through resource consolidation. As core facilities proliferate, national and international networks have emerged that facilitate global knowledge exchange. Here we examine the benefits of establishing local networks of light microscopy facility staff. These small networks complement their larger, formal counterparts by creating a sense of community and offering practical day-to-day benefits, such as rapid technical support, resource sharing, and informal mentorship.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"312-319"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global survey of Flow Cytometry core facilities 2021","authors":"Jessica B Back,&nbsp;Alexander Scott,&nbsp;Zhenxi Guo,&nbsp;Masilamani Elangovan,&nbsp;Kirk J. Czymmek,&nbsp;Peter O'Toole,&nbsp;Derek Davies","doi":"10.1111/jmi.70094","DOIUrl":"10.1111/jmi.70094","url":null,"abstract":"<p>Two surveys were conducted of international core facilities that focused on Microscopy and Flow Cytometry for an in-depth snapshot of the landscape. Here we focus on the Flow Cytometry survey which had 148 respondents across ‘The Americas’, ‘Asia Pacific’ and ‘Europe and Africa’. A variety of subjects were covered, including instrument types and quantities, staffing, fees and funding, and specific questions related to COVID response. Broadly, the global trends were similar but there were some geographical differences notably in staff to machine ratio and funding models. As this serves as a baseline for longitudinal studies, we will be able to use these data to follow trends across all metrics in the future as facilities and technologies evolve.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"434-445"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expertise is the new infrastructure","authors":"Pablo Hernandez-Varas,&nbsp;Chiara Rossi,&nbsp;Erin M. Tranfield,&nbsp;Baubak Bajoghli,&nbsp;Clara Prats,&nbsp;Winnok H. De Vos,&nbsp;Sebastian Munck","doi":"10.1111/jmi.70103","DOIUrl":"10.1111/jmi.70103","url":null,"abstract":"<p>Today's life science endeavours are complex and interconnected, and they face diverse challenges, such as budgetary insecurity and the reproducibility crisis. As a result, core facilities have become vital in research institutes, transitioning from mere conveniences to essential components that ensure instrument access. Core facilities actively support and steer research with staff advising on experimental design and data interpretation. However, core facilities must, likewise, continually demonstrate their value, and there is no clear consensus on their optimal operational structure. Integration into larger, geographically distributed infrastructures represents a crucial opportunity for consolidation and differentiation, provided expertise and focus are clearly defined. Here, we outline concrete steps to emphasise specific application know-how and increase visibility for imaging facilities to become future-proof, improve matchmaking to attract the right users, and build international recognition across European infrastructures. By clearly delineating and differentiating expertise, core facilities can accelerate research and position themselves as integral components of large-scale, durable infrastructures.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"244-255"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress and conflict management in core facilities—News from an imaging survey","authors":"Jan Peychl,&nbsp;Chiara Rossi,&nbsp;Nathalie Aulner,&nbsp;Audrey Salles,&nbsp;Nadia Halidi,&nbsp;Maia Brunstein,&nbsp;Adeline Mallet,&nbsp;Karin Aumayr,&nbsp;Jean-Marc Verbavatz,&nbsp;Thomas Heuser,&nbsp;Rachel Santarella-Mellwig,&nbsp;Aurelien Dauphin,&nbsp;Eef Parthoens,&nbsp;Manuel Gunkel,&nbsp;Martin Fritsch,&nbsp;Sebastian T Bundschuh,&nbsp;Rym Chaabouni,&nbsp;Agnes Uhereczky,&nbsp;Erin M Tranfield,&nbsp;Pablo Hernández Varas,&nbsp;Sebastian Munck,&nbsp;the Core4Life Consortium","doi":"10.1111/jmi.70102","DOIUrl":"10.1111/jmi.70102","url":null,"abstract":"<p>While conflict management is a well-known challenge for people working in the service sector, this topic remains largely unexplored in scientific imaging core facilities, despite frequent interactions with diverse users in environments with complex service demands. To address this gap, we conducted a survey to assess staff experiences, challenges, and the availability of institutional support. The 38-question survey, completed anonymously, covered three areas: (1) respondent background; (2) assessment of stress in core facilities and coping mechanisms and stress management resources; and (3) work environment challenges such as user interactions and overall work atmosphere. The results highlight the need for more awareness, targeted training, and organisational strategies to improve staff well-being and foster a collaborative work culture. By raising awareness and providing actionable insights, we aim to inform best practices for conflict de-escalation and, when necessary, conflict management in research infrastructure settings. While exemplified with scientific imaging facilities, we believe our survey results are easily transferable to other scientific cores and infrastructures with similar service and user interactions. In addition, the dataset is available to other researchers on figshare. Information concerning the data records, usage notes, code availability, and technical validation is presented.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"370-381"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Built to be shared: Lessons from the Canada Foundation for Innovation (CFI) on funding core facilities","authors":"Mark Lagacé,&nbsp;Heidi Bandulet,&nbsp;Natalee Rubec,&nbsp;Olivier Gagnon,&nbsp;Sandra Zohar","doi":"10.1111/jmi.70111","DOIUrl":"10.1111/jmi.70111","url":null,"abstract":"<p>Core facilities constitute an increasingly significant component of research infrastructure at Canadian institutions, providing shared access to specialised equipment, services, and expertise. We present a funder's perspective on the value of core facilities, which the Canada Foundation for Innovation (CFI) has supported for over a decade leading to the recent creation of a dedicated funding stream for core facilities as part of its 2025 Innovation Fund competition. This article details the CFI's consultative approach and steps taken towards the launch of the core facilities funding stream. The new stream's key features include expanded eligibility for scientific and technical personnel support, core facility-specific assessment criteria, and inclusion of experienced core facilities experts in merit review processes. Findings from the 2025 competition indicate that the core facility stream was well-structured and that proposals in this stream had similar or higher success and funding rates than those submitted to the open stream (project-based proposals). The CFI's next steps include enhanced data collection, the development of performance and reporting approaches, and continued refinement of funding mechanisms to better reflect the operational characteristics and system level role of core facilities within Canada's research ecosystem.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"267-273"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global impact through train-the-trainer (TtT) courses","authors":"Mar García-Ferrés,&nbsp;Amy L. Bottomley,&nbsp;Louise Cole,&nbsp;Marcela Díaz,&nbsp;Neftali Flores-Rodriguez,&nbsp;Gleb Grebnev,&nbsp;Adan Guerrero,&nbsp;Caron A. Jacobs,&nbsp;Laurence Lejeune,&nbsp;Xiaoxiao Ma,&nbsp;Leonel Malacrida,&nbsp;Paul J. McMillan,&nbsp;Alison J. North,&nbsp;Cameron J. Nowell,&nbsp;Naomi Okugbeni,&nbsp;María V. Repetto,&nbsp;Alejandra M Ross Beraldi,&nbsp;Andres Hugo Rossi,&nbsp;Lía I. Pietrasanta,&nbsp;Rajan Singh,&nbsp;Claire M. Brown","doi":"10.1111/jmi.70116","DOIUrl":"10.1111/jmi.70116","url":null,"abstract":"<p>Global BioImaging (GBI) and its regional and national partners, such as Canada BioImaging (CBI), BioImaging North America (BINA), Latin America BioImaging (LABI), the African BioImaging Consortium (ABIC) and Microscopy Australia (MA), have been working to create training opportunities for imaging scientists across the globe. During the COVID-19 pandemic, GBI and its partners developed a virtual training platform for independent learning (GBI Virtual Training Platform), with self-guided training modules spanning from microscope operation to facility management. More recently, in-person train-the-trainer (TtT) frameworks, including the Montreal Light Microscopy Course (MLMC), have been designed to deliver a multiplying effect by providing imaging scientists with the training and resources they need to run microscopy courses in their facilities, institutions, countries, and regions. Major goals of this initiative included empowering the next generation of imaging scientists by sharing resources and expertise, providing training in course logistics and organisation and building an international collaborative network of expert trainers to accelerate access to fundamental and advanced technologies. As of April 2026, the TtT project has consisted of three TtT courses, at least 11 second-generation microscopy fundamentals courses and impacted more than 400 course faculty, corporate partners and trainees from 25 countries.</p><p><b>LAY DESCRIPTION</b>: This manuscript demonstrates that global train-the-trainer (TtT) programmes in bioimaging create a powerful multiplier effect, rapidly expanding microscopy training capacity. By equipping imaging scientists with course materials, training, and access to a global trainer's network, it enabled second-generation courses across multiple regions, impacting over 400 individuals in 25 countries. It empowered the next generation and strengthened international collaboration to help democratise access to advanced technologies and expertise to accelerate scientific discovery and innovation. With sufficient resources, TtT is scalable across technology areas and research fields.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"302 3","pages":"396-405"},"PeriodicalIF":1.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The changing landscape of training in microscopy facilities: Reflections on seven decades of research user support at the University of Sydney.","authors":"Eleanor Kable, Matthew Foley, Vijay Bhatia, Filip Braet","doi":"10.1111/jmi.70121","DOIUrl":"https://doi.org/10.1111/jmi.70121","url":null,"abstract":"<p><p>This thematic contribution highlights the critical role of established professional training regimes in microscopy and microanalysis in supporting high-quality research. As microscopy technologies continue to evolve in complexity, the risk of mishandling and data misinterpretation by inexperienced researchers increases significantly. To address this challenge, the authors underscore the importance of expert training and ongoing support provided by platform scientists-specialist microscopists embedded within centralised research infrastructure facilities. These experts play a vital role in equipping researchers with the necessary skills to use advanced instruments effectively and interpret data accurately. Collaboration between platform scientists and researchers is not only beneficial but essential for maximising the capabilities of modern microscopy. By fostering such partnerships and investing in structured training solutions, institutions can ensure that scientific discoveries are grounded in reliable, high-quality imaging data, advancing the broader goals of research excellence. At the microscopy core facility in Sydney, we have found that no single training method can fully substitute the comprehensive researcher experience required to become an expert microscopy scientist. Therefore, we suggest that a hybrid approach-combining multiple training tools with regular researcher check-ins throughout the research project lifecycle-is the most effective solution.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}