ApoNecV: A macro for cell death type differentiation

IF 1.5 4区 工程技术 Q3 MICROSCOPY
Marketa Kolarikova, Barbora Hosikova, Jiri Tesarik, Katerina Langova, Hana Kolarova
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引用次数: 0

Abstract

The evaluation of large experimental datasets is a fundamental aspect of research in every scientific field. Streamlining this process can improve the reliability of results while making data analysis more efficient and faster to execute. In biomedical research it is often very important to determine the type of cell death after various treatments. Thus, differentiating between viable, apoptotic, and necrotic cells provide critical insights into the treatment efficacy, a key aspect in the field of drug development. Fluorescent microscopy is perceived as a widely used technique for cell metabolism assessment and can therefore be used to investigate treatment outcomes after staining samples with cell death detection kit. However, accurate evaluation of therapeutic results requires quantitative analysis, often necessitating extensive postprocessing of imaging data. In this study, we introduce a complementary tool designed as a macro for the Fiji platform, enabling the automated postprocessing of fluorescent microscopy images to accurately distinguish and quantify viable, apoptotic, and necrotic cells.

Abstract Image

ApoNecV:细胞死亡类型分化的宏。
大型实验数据集的评估是每个科学领域研究的一个基本方面。简化此过程可以提高结果的可靠性,同时使数据分析更有效,执行速度更快。在生物医学研究中,确定各种治疗后细胞死亡的类型往往是非常重要的。因此,区分活细胞、凋亡细胞和坏死细胞提供了对治疗效果的重要见解,这是药物开发领域的一个关键方面。荧光显微镜被认为是一种广泛使用的细胞代谢评估技术,因此可以用来研究细胞死亡检测试剂盒染色样品后的治疗结果。然而,准确评估治疗结果需要定量分析,通常需要对成像数据进行大量的后处理。在这项研究中,我们引入了一种辅助工具,设计为斐济平台的宏,使荧光显微镜图像的自动后处理能够准确区分和量化活细胞,凋亡细胞和坏死细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of microscopy
Journal of microscopy 工程技术-显微镜技术
CiteScore
4.30
自引率
5.00%
发文量
83
审稿时长
1 months
期刊介绍: The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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