Journal of microscopy最新文献

Traction force generation in Plasmodium sporozoites is modulated by a surface adhesin. 疟原虫孢子产生的牵引力是由表面黏附素调节的。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-07-26 DOI: 10.1111/jmi.70015

Johanna Ripp, Dimitri Probst, Mirko Singer, Ulrich S Schwarz, Friedrich Frischknecht

{"title":"Traction force generation in Plasmodium sporozoites is modulated by a surface adhesin.","authors":"Johanna Ripp, Dimitri Probst, Mirko Singer, Ulrich S Schwarz, Friedrich Frischknecht","doi":"10.1111/jmi.70015","DOIUrl":"https://doi.org/10.1111/jmi.70015","url":null,"abstract":"Plasmodium sporozoites are the highly polarised and motile forms of the malaria parasite transmitted by mosquitoes to the vertebrate hosts. Sporozoites use myosin motors to generate retrograde flow of actin filaments. These are linked to plasma membrane spanning adhesins, which in turn bind to the extracellular environment, resulting in forward directed gliding motility. The gliding motility machine of sporozoites leads to high speeds in the range of micrometres per second, which are essential for efficient migration in the skin. Yet, it is not clear how the individual parts of the machinery work together to generate force during migration. Sporozoites are elongated and curved cells and move on circular tracks in vitro. Sporozoites lacking the adhesin thrombospondin-related anonymous protein (TRAP) like protein, TLP, can still migrate in the skin, but at a lower level. TLP lacking sporozoites generate a lower force on the dorsal (nonsubstrate facing) surface as measured by laser tweezers. Here we use traction force microscopy to investigate motile sporozoites and the forces they produce during migration on their ventral surface. Both wild type and tlp(-) sporozoites show distinct foci of force generation, but tlp(-) sporozoites generating overall lower forces. Our findings demonstrate that TLP is an important element of the force-generating machinery during sporozoite gliding motility.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability of electron ptychography at low electron dose. 低电子剂量下电子型图的稳定性。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-07-26 DOI: 10.1111/jmi.70011

M Dearg, N Michaelides, J Gilbert, Z Ding, Z Aslam, D G Hopkinson, C S Allen, L Clark

引用次数: 0

Visualisation and differentiation of binder components in hard carbon composite anodes by osmium tetroxide and uranyl acetate staining. 用四氧化锇和醋酸铀酰染色观察和区分硬质碳复合材料阳极中粘结剂成分。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-21 DOI: 10.1111/jmi.70014

Gregor Neusser, Tom Philipp, Christine Kranz

{"title":"Visualisation and differentiation of binder components in hard carbon composite anodes by osmium tetroxide and uranyl acetate staining.","authors":"Gregor Neusser, Tom Philipp, Christine Kranz","doi":"10.1111/jmi.70014","DOIUrl":"https://doi.org/10.1111/jmi.70014","url":null,"abstract":"In this study, we present a protocol to visualise, track and distinguish between two different binder components commonly used for batteries, styrene butadiene rubber (SBR) and sodium carboxymethyl cellulose (CMC), within a composite hard carbon electrode for sodium-ion batteries using a two-step staining method. The application of osmium tetroxide (OsO4) vapour followed by uranyl acetate (UA) solution enables the staining of different functional groups and the individual tracing of SBR and CMC by energy dispersive X-ray spectroscopy (EDX) measurements using the osmium (Os) and uranium (U) content. This staining procedure and the filling of the pore space with conductive platinum carbon (PtC) composite via local electron-beam-induced deposition (EBID) results in an excellent contrast for all components of the electrode material. The tracking and visualisation of the binder components are demonstrated with secondary electron (SE) imaging and EDX mappings at focused ion beam (FIB) prepared facets as well as with focused ion beam/scanning electron microscopy (FIB/SEM) tomography. LAY DESCRIPTION: In this study, a sample preparation protocol for hard carbon (HC) composite electrode material is presented which allows to clearly distinguish between the HC particle and the two binder components, styrene butadiene rubber (SBR) and sodium carboxymethyl cellulose (CMC) in focused ion beam/scanning electron microscopy (FIB/SEM) tomography and energy dispersive X-ray spectroscopy (EDX) measurements. For that, the material was stained with osmium tetroxide (OsO4) and uranyl acetate (UA) and pore space was locally filled with electron-beam-induced deposition (EBID) of platinum carbon (PtC).","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TOC - Issue Information TOC -发布信息

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-16 DOI: 10.1111/jmi.13329

引用次数: 0

Fast Gaussian fitting peak extraction for confocal microscopy. 用于共聚焦显微镜的快速高斯拟合峰提取。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-16 DOI: 10.1111/jmi.70012

Jian Liu, Zhenlong Xu, Ziyi Wang, Xiaoyu You, Chenguang Liu

引用次数: 0

Influence of polarisation states on the axial point spread function in subtractive second harmonic generation microscopy with a vortex beam. 偏振态对涡旋光束相减二次谐波产生显微镜轴向点扩展函数的影响。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-15 DOI: 10.1111/jmi.70013

Huizhen Xu, Kanwarpal Singh

引用次数: 0

Ptychographic analysis of human bone marrow-derived mesenchymal stem cell morphology: The impact of cell senescence. 人骨髓源间充质干细胞形态的病理学分析：细胞衰老的影响。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-11 DOI: 10.1111/jmi.70003

Lorenzo Anconelli, Giovanna Farruggia, Isabella Zafferri, Francesca Borsetti, Stefano Iotti, Francesca Rossi, Jeanette A Maier

{"title":"Ptychographic analysis of human bone marrow-derived mesenchymal stem cell morphology: The impact of cell senescence.","authors":"Lorenzo Anconelli, Giovanna Farruggia, Isabella Zafferri, Francesca Borsetti, Stefano Iotti, Francesca Rossi, Jeanette A Maier","doi":"10.1111/jmi.70003","DOIUrl":"https://doi.org/10.1111/jmi.70003","url":null,"abstract":"Mesenchymal stem cells (MSC) undergo replicative senescence, a state of irreversible cell cycle arrest that limits their utility in regenerative medicine applications. To identify novel markers of senescence useful to enhance the quality of MSC-based therapies, we compared young and senescent human bone marrow-derived mesenchymal stem cells (hMSCs) using a non-invasive, label-free approach based on quantitative phase imaging (QPI) with the Livecyte microscope. Senescent hMSCs demonstrated substantial morphological alterations, including a threefold increase in cell area, elevated dry mass, reduced thickness, and decreased sphericity compared to their younger counterparts. Additionally, motility metrics such as instantaneous velocity and displacement were significantly reduced in senescent cells, underscoring functional impairments that could hinder their therapeutic potential in regenerative medicine. The application of QPI offers a promising tool for monitoring cellular health, identifying, and potentially eliminating, senescent cells to improve the quality and effectiveness of MSC-based therapies.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of reliable and representative DVC measurements for musculoskeletal tissues. 可靠和代表性的肌肉骨骼组织DVC测量的概述。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-10 DOI: 10.1111/jmi.70008

Gianluca Tozzi, Enrico Dall'Ara

{"title":"An overview of reliable and representative DVC measurements for musculoskeletal tissues.","authors":"Gianluca Tozzi, Enrico Dall'Ara","doi":"10.1111/jmi.70008","DOIUrl":"https://doi.org/10.1111/jmi.70008","url":null,"abstract":"Musculoskeletal tissues present complex hierarchical structures and mechanical heterogeneity across multiple length scales, making them difficult to characterise accurately. Digital volume correlation (DVC) is a non-destructive imaging technique that enables quantification of internal 3D strain fields under realistic loading conditions, offering a unique tool to investigate the biomechanics of biological tissues and biomaterials. This review highlights recent advancements in DVC, focusing on its applications across scales ranging from organ- to tissue-level mechanics in both mineralised and soft tissues. Instead of a traditional systematic review, we identify key technical challenges including the treatment of tissue interfaces, border effects, and the quantification of uncertainty in DVC outputs. Strategies for improving measurement accuracy and reliability are discussed. We also report on the increasing use of DVC in in vivo applications, its coupling with computational modelling to inform and validate biomechanical simulations, and its recent integration with data-driven methods such as deep learning to directly predict displacement and strain fields. Additionally, we examine its application in tissue engineering and implant-tissue interface assessment. By addressing such areas, we outline current limitations and emerging opportunities for future research. These include advancing precision, enabling clinical translation, and leveraging machine learning to create more robust, automated, and predictive DVC workflows for musculoskeletal health and tissue engineering.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on critique and rebuttal of ‘On the pixel selection criterion for the calculation of the Pearson's correlation coefficient in fluorescence microscopy’ 对《关于荧光显微镜中皮尔逊相关系数计算的像素选择标准》一文的批判和反驳的评论。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-09 DOI: 10.1111/jmi.70006

Fabrice Cordelieres, Michelle Peckham

引用次数: 0

Response to Adler and Parmryd 对Adler和Parmryd的回应。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2025-07-09 DOI: 10.1111/jmi.13428

Sergio G. Lopez, Sebastian Samwald, Sally Jones, Christine Faulkner

引用次数: 0