Journal of microscopy最新文献

Surface visualisation of bacterial biofilms using neutral atom microscopy. 用中性原子显微镜观察细菌生物膜的表面。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-10-03 DOI: 10.1111/jmi.70038

Nick A von Jeinsen, David J Ward, Matthew Bergin, Sam M Lambrick, David M Williamson, Richard M Langford, Lisa F Dawson, Vibhuti Rana, Sushma Shivaswamy, Xuening Zhou, Michelle Mikesh, Vernita D Gordon, Brendan W Wren, Katherine A Brown, Paul C Dastoor

引用次数: 0

A fluorescence lifetime separation approach for FLIM live-cell imaging. FLIM活细胞成像的荧光寿命分离方法。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-30 DOI: 10.1111/jmi.70036

Cornelia Wetzker, Marcelo Leomil Zoccoler, Svetlana Iarovenko, Chukwuebuka William Okafornta, Anja Nobst, Hella Hartmann, Thomas Müller-Reichert, Robert Haase, Gunar Fabig

{"title":"A fluorescence lifetime separation approach for FLIM live-cell imaging.","authors":"Cornelia Wetzker, Marcelo Leomil Zoccoler, Svetlana Iarovenko, Chukwuebuka William Okafornta, Anja Nobst, Hella Hartmann, Thomas Müller-Reichert, Robert Haase, Gunar Fabig","doi":"10.1111/jmi.70036","DOIUrl":"https://doi.org/10.1111/jmi.70036","url":null,"abstract":"Fluorescence lifetime imaging microscopy (FLIM) translates the duration of excited states of fluorophores into lifetime information as an additional source of contrast in images of biological samples. This offers the possibility to separate fluorophores particularly beneficial in case of similar excitation spectra. Here, we demonstrate the distinction of fluorescent molecules based on FLIM phasor analysis, called lifetime separation, in live-cell imaging using open-source software for analysis. We showcase two applications using Caenorhabditis elegans as a model system. First, we separated the highly spectrally overlapping fluorophores mCherry and mKate2 to distinctively track tagged proteins in six-dimensional datasets to investigate cell division in the developing early embryo. Second, we separated fluorescence of tagged proteins of interest from masking natural autofluorescence in adult hermaphrodites. For FLIM data handling and workflow implementation, we developed the open-source plugin napari-flim-phasor-plotter to implement conversion, visualisation, analysis and reuse of FLIM data of different formats. Our work thus advances technical applications and bioimage data management and analysis in FLIM microscopy for life science research.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of near-field optical microscopy and tip-assisted photoluminescence. 近场光学显微镜与尖端辅助光致发光的相关性。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-27 DOI: 10.1111/jmi.70037

W Pfeiffer, N S Mueller, R Hillenbrand, I Niehues, P Kusch

{"title":"Correlation of near-field optical microscopy and tip-assisted photoluminescence.","authors":"W Pfeiffer, N S Mueller, R Hillenbrand, I Niehues, P Kusch","doi":"10.1111/jmi.70037","DOIUrl":"https://doi.org/10.1111/jmi.70037","url":null,"abstract":"Nanoscale optical imaging has unlocked unprecedented opportunities for exploring the structural, electronic, and optical properties of low-dimensional materials with spatial resolutions far beyond the diffraction limit. Techniques such as tip-enhanced, and tip-assisted photoluminescence (TEPL and TAPL), as well as scattering-type scanning near-field optical microscopy (s-SNOM) offer unique insights into local strain distributions, exciton dynamics, and dielectric heterogeneities that are inaccessible through conventional far-field approaches, however their combination within the same setup remains challenging. Here we present the realisation of correlative TEPL/TAPL and s-SNOM measurements within a single side-illuminated near-field optical microscope. We address the key experimental challenges inherent to the side-illumination geometry, including precise laser focus alignment, suppression of far-field background signals, and the mitigation of competing scattering pathways. Utilising monolayer WSe2 as a model system, we demonstrate correlative imaging of material topography, strain-induced photoluminescence shifts, and dielectric function variations. We visualise nanoscale heterogeneities on a bubble-like structure, highlighting the complementary information from TAPL and s-SNOM. This correlative approach bridges the gap between nanoscale optical spectroscopy and near-field imaging, offering a powerful tool for probing local strain, doping, exciton behaviour, and dielectric inhomogeneities in low-dimensional materials.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145175637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TOC - Issue Information TOC -发布信息

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-24 DOI: 10.1111/jmi.70033

引用次数: 0

Microstructural characterisation of polycrystalline ice with an etch-pitting replication method. 用蚀刻点蚀复制法表征多晶冰的微观结构。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-23 DOI: 10.1111/jmi.70031

Hatsuki Yamauchi, Lucy Davidson, Christine McCarthy, David J Prior, Jacob A Tielke, Benjamin K Holtzman

{"title":"Microstructural characterisation of polycrystalline ice with an etch-pitting replication method.","authors":"Hatsuki Yamauchi, Lucy Davidson, Christine McCarthy, David J Prior, Jacob A Tielke, Benjamin K Holtzman","doi":"10.1111/jmi.70031","DOIUrl":"https://doi.org/10.1111/jmi.70031","url":null,"abstract":"Etch-pitting replication is a classical method to characterise the microstructure of ice crystals. In this method, a solution of polyvinyl formal (Formvar) is applied to a polished surface of ice. A plastic film, created after the solvent is dried, 'replicates' microstructural features of the ice. By examining the replica film, we can identify the orientation of crystals and existence of dislocations in ice. However, with the recent rise of advanced techniques such as cryo-EBSD (electron backscatter diffraction) analyses, this classical method has been left in the shadows, especially from the perspective of quantification of microstructural features in polycrystalline ice. In this study we revive and thoroughly re-examine the utility of the replication method to quantify crystal orientations and dislocation density of ice. We applied our optimised protocols of the replication method to several laboratory-fabricated and natural-glacier polycrystalline ice samples with various types of crystal preferred orientation (CPO) and various levels of strain. Using high-resolution scanning electron microscope (SEM) images of the obtained replica films, we quantified the extent of CPO and dislocation density of these ice samples. Our results of CPO patterns and dislocation density show good agreement with cryo-EBSD results from the same ice samples or samples at a similar strain level. Although further improvements are needed to make the present method more efficient, our results show promise for using this method to easily, quickly, and affordably quantify microstructural features in polycrystalline ice and to help interpret deformation mechanism of ice.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-18 DOI: 10.1111/jmi.70035

Rafael Nadas, Lucas Liberal, Gabriel Bargas, Kenji Watanabe, Takashi Taniguchi, Leonardo C Campos, Ado Jorio

{"title":"<ArticleTitle xmlns:ns0=\"http://www.w3.org/1998/Math/MathML\">Substrate-related optical activity in monolayer <ns0:math> <ns0:semantics><ns0:msub><ns0:mi>WS</ns0:mi> <ns0:mn>2</ns0:mn></ns0:msub> <ns0:annotation>${rm WS}_2$</ns0:annotation></ns0:semantics> </ns0:math> and <ns0:math> <ns0:semantics><ns0:msub><ns0:mi>MoSe</ns0:mi> <ns0:mn>2</ns0:mn></ns0:msub> <ns0:annotation>${rm MoSe}_2$</ns0:annotation></ns0:semantics> </ns0:math> : A tip-enhanced Raman spectroscopy study.","authors":"Rafael Nadas, Lucas Liberal, Gabriel Bargas, Kenji Watanabe, Takashi Taniguchi, Leonardo C Campos, Ado Jorio","doi":"10.1111/jmi.70035","DOIUrl":"https://doi.org/10.1111/jmi.70035","url":null,"abstract":"Transition metal dichalcogenides (TMDs) are promising two-dimensional materials whose properties are strongly influenced by substrate interactions. While conventional Raman spectroscopy probes these effects, its diffraction-limited resolution often averages out local variations such as strain, masking intrinsic behaviours. Here, we employ tip-enhanced Raman spectroscopy (TERS) to investigate the vibrational properties of monolayer <math> <semantics><msub><mi>WS</mi> <mn>2</mn></msub> <annotation>${rm WS}_2$</annotation></semantics> </math> and <math> <semantics><msub><mi>MoSe</mi> <mn>2</mn></msub> <annotation>${rm MoSe}_2$</annotation></semantics> </math> on top of glass and glass/hBN substrates. TERS offers nanometric spatial resolution, allowing direct correlation between Raman features and topographical inhomogeneities. Our results reveal that local variations in strain, doping, and dielectric screening that vary across the substrate interface are often accompanied by nanoscale structural features such as wrinkles, which locally modulate the vibrational response.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analogue optical pattern recognition for cross-correlational CNN. 交叉相关CNN的模拟光学模式识别。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-15 DOI: 10.1111/jmi.70034

Ahmed Farhat, Wim J C Melis

引用次数: 0

Leveraging modified ex situ tomography data for segmentation of in situ synchrotron X-ray computed tomography. 利用改进的非原位断层扫描数据进行原位同步加速器x射线计算机断层扫描的分割。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-12 DOI: 10.1111/jmi.70032

Tristan Manchester, Adam Anders, Julio Spadotto, Hannah Eccleston, William Beavan, Hugues Arcis, Brian J Connolly

{"title":"Leveraging modified ex situ tomography data for segmentation of in situ synchrotron X-ray computed tomography.","authors":"Tristan Manchester, Adam Anders, Julio Spadotto, Hannah Eccleston, William Beavan, Hugues Arcis, Brian J Connolly","doi":"10.1111/jmi.70032","DOIUrl":"https://doi.org/10.1111/jmi.70032","url":null,"abstract":"In situ synchrotron X-ray computed tomography enables dynamic material studies. However, automated segmentation remains challenging due to complex imaging artefacts - like ring and cupping effects - and limited training data. We present a methodology for deep learning-based segmentation by transforming high-quality ex situ laboratory data to train models for segmentation of in situ synchrotron data, demonstrated through a metal oxide dissolution study. Using a modified SegFormer architecture, our approach achieves segmentation performance (94.7% IoU) that matches human inter-annotator reliability (94.6% IoU). This indicates the model has reached the practical upper bound for this task, while reducing processing time by 2 orders of magnitude per 3D dataset compared to manual segmentation. The method maintains robust performance over significant morphological changes during experiments, despite training only on static specimens. This methodology can be readily applied to diverse materials systems, enabling the efficient analysis of the large volumes of time-resolved tomographic data generated in typical in situ experiments across scientific disciplines.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crossing scales and eras: Correlative multimodal microscopy heritage studies. 跨越尺度和时代：相关的多模态显微镜遗产研究。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-09-12 DOI: 10.1111/jmi.70030

Charles Wood, George Deakin, Atousa Moayedi, Jovana Radulovic

{"title":"Crossing scales and eras: Correlative multimodal microscopy heritage studies.","authors":"Charles Wood, George Deakin, Atousa Moayedi, Jovana Radulovic","doi":"10.1111/jmi.70030","DOIUrl":"https://doi.org/10.1111/jmi.70030","url":null,"abstract":"The comprehensive characterisation of complex, irreplaceable cultural heritage artefacts presents significant challenges for traditional analytical methods, which can fall short in providing multi-scale, non-invasive analysis. Correlative Multimodal Microscopy (CoMic), an approach that integrates data from multiple techniques, offers a powerful solution by bridging structural, chemical, and topographical information across different length scales. This paper provides a comprehensive review of the evolution, current applications, and future trajectory of CoMic within the field of heritage science. We present a historical overview of microscopy in heritage studies and detail the principles and advances of key techniques, such as electron, X-ray, optical, and probe microscopies. This review presents practical applications through case studies on materials that include wood, pigments, ceramics, metals, and textiles. To aid CoMic uptake, we also provide user-centric guides for researchers with diverse expertise. This review also examines the challenges that currently limit the widespread adoption of CoMic, challenges that include sample preparation, data correlation accuracy, high instrumental and resource costs, and the need for specialised interdisciplinary expertise. Although CoMic is a transformative methodology for artefact analysis and conservation, its full potential will be realised through future developments in accessible instrumentation, standardised protocols, and the integration of AI-driven data analysis. This review serves as a critical resource and roadmap for researchers, conservators, and institutions looking to harness the power of correlative microscopy to preserve our shared cultural legacy.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring collagen fibrillogenesis at the nanoscale: Tip-enhanced Raman imaging of protofibrils. 在纳米尺度上探索胶原纤维的形成：原纤维的尖端增强拉曼成像。

IF 1.9 4区工程技术

Journal of microscopy Pub Date : 2025-08-29 DOI: 10.1111/jmi.70029

Maria A Paularie, Emerson A Fonseca, Vitor Monken, André G Pereira, Rafael P Vieira, Ado Jorio

引用次数: 0