Journal of microscopy最新文献

{"title":"Comparative morphological characterisation of SARS-CoV-2 and influenza B virus using atomic force microscopy.","authors":"Junru Wang, Nan Li, Yujuan Chen, Jingyu Wang, Junxi Wang, Chunyang Gao, Jing Hu, Zuobin Wang","doi":"10.1111/jmi.13432","DOIUrl":"https://doi.org/10.1111/jmi.13432","url":null,"abstract":"<p><p>Influenza B virus and SARS-CoV-2 virus are the two most representative respiratory infectious diseases. These two viruses not only show similarities in clinical symptoms but also have numerous similarities in microstructure, which is difficult to distinguish and poses great challenges for diagnosis. In this work, the three-dimensional structures and surface features of influenza B virus and SARS-CoV-2 virus were investigated using atomic force microscopy. The results indicated that there were substantial differences in surface morphology and structure between the two viruses. Specifically, the average diameter of SARS-CoV-2 virus particles was around 222.8 nm while that of influenza B virus particles is smaller at about 191.2 nm. The height of SARS-CoV-2 particles was also larger, averaging about 30-60 nm, while that of influenza B virus particles averaged around 10-30 nm. Additionally, the crown-like structure on the surface of the SARS-CoV-2 virus was sparser and more prominent than that of the influenza virus. These findings offer significant insights into the distinction between the two viruses, aiding in the accurate characterisation of SARS-CoV-2 and influenza viruses and facilitating timely and effective treatment strategies.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile electrochemical synthesis of binder-free tin nanostructures on carbon foam: A promising electrode for high-efficiency supercapacitors.","authors":"Muhammad Zainul Abadin, Ahtisham Abdul Wahid, Muhammad Usman","doi":"10.1111/jmi.13427","DOIUrl":"https://doi.org/10.1111/jmi.13427","url":null,"abstract":"<p><p>Energy storage technologies that are efficient are in constant demand. Supercapacitors have attracted much interest among these gadgets because of their superior cycle stability and high-power density. This work used a simple and cost-effective sonication-assisted electrodeposition approach to develop tin oxide nanoparticles on functionalised carbon foam substrate with different concentration ratios (1 mM, 3 mM, and 5 mM). FTIR, XRD, and SEM validated the chemical, structural, and morphological characteristics of all nanostructured electrodes. The tetragonal structure with spherical shape was the result of the fine crystallisation of the tin oxide nanoparticles. The electrochemical characteristics are evaluated by CV, EIS, and GCD testing. Among all electrodes, Sn₁@CF has a larger electrochemically active surface area, low internal resistance, and high specific capacitance. These findings underscore that the binder-free Sn₁@CF electrode is a promising candidate for high-efficiency supercapacitor applications.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of large angle polepiece on spherical aberration coefficient.","authors":"Jincan Su, Xiaotian Hu, Tao Hu, Shigang Dong, Jian Zhang, Yucheng Liu, Faguo Chen, Bingtao Zhang","doi":"10.1111/jmi.13430","DOIUrl":"https://doi.org/10.1111/jmi.13430","url":null,"abstract":"<p><p>X-rays, secondary electrons, and other emitted electrons need to be extracted at a large solid angle to enhance electron collection efficiency in transmission electron microscopy. The finite element method is employed to investigate the effects of different polepiece angles on the spherical aberration coefficient of polepiece. The research findings reveal that the azimuthal angle β of the upper polepiece has a substantial effect on the spherical aberration coefficient. When β = 30°, the minimum spherical aberration coefficient is achieved. When β ≥ 50°, the spherical aberration coefficient increases significantly, which adversely affects imaging. The aperture size of the upper polepiece has a relatively minor effect on the spherical aberration. The design of the large-angle polepiece offers novel design concepts for future emission X-ray/electron collection devices, while also offering a new reference for the design of objective lenses in transmission electron microscopy.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple Python-based methods for analysis and drift-correction of STM images.","authors":"Francesco Cazzadori, Alessandro Facchin, Silvio Reginato, Christian Durante","doi":"10.1111/jmi.13426","DOIUrl":"https://doi.org/10.1111/jmi.13426","url":null,"abstract":"<p><p>A successful scanning tunnelling microscopy (STM) experiment relies on both delicate sample preparation and measurement, and careful image filtering and analysis to provide clear and solid results. Processing and analysis of STM images may result in a tricky task, due to the complexity and specificity of the probed systems. In this paper, we introduce our recently developed, simple Python-based methods for filtering and analysing STM images, with the aim of providing a semi-quantitative treatment of the input data. Case studies will be presented using images obtained through electrochemical STM. Additionally, we propose a straightforward yet effective universal drift-correction tool for SPM image sequences.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PerfectlyAverage: A classical open-source software method to determine the optimal averaging parameters in laser scanning fluorescence microscopy.","authors":"S Foylan, L M Rooney, W B Amos, G W Gould, G McConnell","doi":"10.1111/jmi.13425","DOIUrl":"https://doi.org/10.1111/jmi.13425","url":null,"abstract":"<p><p>Laser scanning fluorescence microscopy (LSFM) is a widely used imaging method, but image quality is often degraded by noise. Averaging techniques can enhance the signal-to-noise ratio (SNR), but while this can improve image quality, excessive frame accumulation can introduce photobleaching and may lead to unnecessarily long acquisition times. A classical software method called PerfectlyAverage is presented to determine the optimal number of frames for averaging in LSFM using SNR, photobleaching, and power spectral density (PSD) measurements. By assessing temporal intensity variations across frames in a time series, PerfectlyAverage identifies the point where additional averaging ceases to provide significant noise reduction. Experiments with fluorescently stained tissue paper and fibroblast cells validated the approach, demonstrating that up to a fourfold reduction in averaging time may be possible. PerfectlyAverage is open source, compatible with any LSFM data, and it is aimed at improving imaging workflows while reducing the reliance on subjective criteria for choosing the number of averages.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a deep learning method for phase retrieval image enhancement in phase contrast microcomputed tomography.","authors":"Xiao Fan Ding, Xiaoman Duan, Naitao Li, Zahra Khoz, Fang-Xiang Wu, Xiongbiao Chen, Ning Zhu","doi":"10.1111/jmi.13419","DOIUrl":"https://doi.org/10.1111/jmi.13419","url":null,"abstract":"<p><p>Propagation-based imaging (one method of X-ray phase contrast imaging) with microcomputed tomography (PBI-µCT) offers the potential to visualise low-density materials, such as soft tissues and hydrogel constructs, which are difficult to be identified by conventional absorption-based contrast µCT. Conventional µCT reconstruction produces edge-enhanced contrast (EEC) images which preserve sharp boundaries but are susceptible to noise and do not provide consistent grey value representation for the same material. Meanwhile, phase retrieval (PR) algorithms can convert edge enhanced contrast to area contrast to improve signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) but usually results to over-smoothing, thus creating inaccuracies in quantitative analysis. To alleviate these problems, this study developed a deep learning-based method called edge view enhanced phase retrieval (EVEPR), by strategically integrating the complementary spatial features of denoised EEC and PR images, and further applied this method to segment the hydrogel constructs in vivo and ex vivo. EVEPR used paired denoised EEC and PR images to train a deep convolutional neural network (CNN) on a dataset-to-dataset basis. The CNN had been trained on important high-frequency details, for example, edges and boundaries from the EEC image and area contrast from PR images. The CNN predicted result showed enhanced area contrast beyond conventional PR algorithms while improving SNR and CNR. The enhanced CNR especially allowed for the image to be segmented with greater efficiency. EVEPR was applied to in vitro and ex vivo PBI-µCT images of low-density hydrogel constructs. The enhanced visibility and consistency of hydrogel constructs was essential for segmenting such material which usually exhibit extremely poor contrast. The EVEPR images allowed for more accurate segmentation with reduced manual adjustments. The efficiency in segmentation allowed for the generation of a sizeable database of segmented hydrogel scaffolds which were used in conventional data-driven segmentation applications. EVEPR was demonstrated to be a robust post-image processing method capable of significantly enhancing image quality by training a CNN on paired denoised EEC and PR images. This method not only addressed the common issues of over-smoothing and noise susceptibility in conventional PBI-µCT image processing but also allowed for efficient and accurate in vitro and ex vivo image processing applications of low-density materials.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TOC - Issue Information","authors":"","doi":"10.1111/jmi.13327","DOIUrl":"https://doi.org/10.1111/jmi.13327","url":null,"abstract":"","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"298 3","pages":"245-246"},"PeriodicalIF":1.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13327","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confinement effects on the self-assembly behaviour of an amphiphilic quinonoid zwitterion at the liquid-solid interface.","authors":"Lihua Yu, Yuan Fang, Steven De Feyter","doi":"10.1111/jmi.13421","DOIUrl":"https://doi.org/10.1111/jmi.13421","url":null,"abstract":"<p><p>Supramolecular self-assembly on surfaces enables tailored interfaces with applications in nanotechnology. While factors like temperature and solute concentration influence self-assembled molecular networks (SAMNs), the role of spatial confinement remains less explored. Here, we investigate the self-assembly of an alkylated quinonoid zwitterion (QZ-C16) at the liquid-solid interface using scanning tunnelling microscopy (STM), both in in situ as well as ex situ nanocorrals. Engineered nanocorrals not only provide a confined environment for molecular assembly, but also serve as platforms for probing the impact of geometric constraints on self-assembly behaviour. Understanding the intricate dynamics of self-assembly at the nanoscale, particularly the mechanisms by which confinement influences structural organisation, can inform strategies for achieving desired molecular architectures.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulated electrochemical force microscopy: Investigation of sodium-ion transport at hard carbon composite anodes.","authors":"Sven Daboss, Nikolas Franke, Beatrice Fraboni, Christine Kranz, Tobias Cramer","doi":"10.1111/jmi.13417","DOIUrl":"https://doi.org/10.1111/jmi.13417","url":null,"abstract":"<p><p>For sodium (Na)-ion batteries (SIBs), the next generation of sustainable batteries, hard carbon (HC) composite electrodes are the most used anodes. Here, we demonstrate the potential of modulated electrochemical force microscopy (mec-AFM) to investigate electrochemical strain due to ion insertion at the electrolyte/electrode interface. HC composite anodes have a complex, multiphase structure, which include the HC particles, conductive carbon nanoparticles (carbon black) and the binder. To address the effect of the composite material on the sodium-ion transport, we employ mec-AFM. A HC composite anode was embedded in an epoxy-polymer matrix and was polished to expose a micro-sized area that enabled high-frequency modulation of the ion transport. We analyse the influence of the modulation on interfacial forces and its role in generating electrochemical strain in the composite anode. Multichannel mec-AFM imaging at varying electrode potentials revealed that the observed electrochemical strain predominantly occurred in the softer binder matrix rather than in the HC microparticles. Our findings underscore the significance of ionic transport pathways through the binder matrix and establish mec-AFM as a novel AFM-derived technique for visualising ion dynamics at battery interfaces.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryo-SEM in haematological research.","authors":"Irina Davidovich, Carina Levin, Yeshayahu Talmon","doi":"10.1111/jmi.13424","DOIUrl":"https://doi.org/10.1111/jmi.13424","url":null,"abstract":"<p><p>Cryogenic scanning electron microscopy (cryo-SEM) is a powerful imaging technique used in cellular biology, providing high-resolution micrographs that show the complexity and dynamics of biological systems. The use of high-pressure freezing (HPF) for specimen fixation preserves cellular structures in their native, hydrated state, avoiding the artefacts introduced by conventional chemical fixation, while modern microscopes provide high-resolution imaging at low electron acceleration voltage, giving fine structural details. That makes cryo-SEM a unique tool for understanding cellular complexity. However, operating the SEM at cryogenic conditions requires careful optimisation of working parameters to avoid artefacts. In our work, we explore the potential of cryo-SEM for haematology and general cell studies. We discuss the impact of a combination of different signals and work distance on specimen appearance and present examples of studies on healthy human blood cells under physiological conditions. Our findings illustrate the breadth of information that can be obtained from these data, highlighting the technique's capacity to enhance our understanding of cellular biology.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}