{"title":"Influence of polarisation states on the axial point spread function in subtractive second harmonic generation microscopy with a vortex beam.","authors":"Huizhen Xu, Kanwarpal Singh","doi":"10.1111/jmi.70013","DOIUrl":null,"url":null,"abstract":"<p><p>Doughnut-shaped vortex beams are widely used to enhance lateral resolution in super-resolution fluorescence microscopy and subtractive second harmonic generation microscopy. The influence of polarisation states on the axial point spread function is investigated theoretically and experimentally in subtractive second harmonic generation microscopy using a first-order Laguerre-Gaussian vortex beam. The influence of left-handed circular, right-handed circular and linear polarised states are analysed for second harmonic generation imaging and compared with results of fluorescence imaging. The results exhibit great agreement with theoretical predictions, and demonstrate the superiority of left-handed circular polarisation in achieving a complete dark central spot and an extended axial point spread function.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microscopy","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1111/jmi.70013","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROSCOPY","Score":null,"Total":0}
引用次数: 0
Abstract
Doughnut-shaped vortex beams are widely used to enhance lateral resolution in super-resolution fluorescence microscopy and subtractive second harmonic generation microscopy. The influence of polarisation states on the axial point spread function is investigated theoretically and experimentally in subtractive second harmonic generation microscopy using a first-order Laguerre-Gaussian vortex beam. The influence of left-handed circular, right-handed circular and linear polarised states are analysed for second harmonic generation imaging and compared with results of fluorescence imaging. The results exhibit great agreement with theoretical predictions, and demonstrate the superiority of left-handed circular polarisation in achieving a complete dark central spot and an extended axial point spread function.
期刊介绍：
The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit.
The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens.
Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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