背景去除器-处理有噪声的显微镜图像的有效工具。

IF 1.9 4区 工程技术 Q3 MICROSCOPY
A. Kilian, P. Bilski, M. Sankowska
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引用次数: 0

摘要

背景去除器(BGR)是一种新型的软件工具,作为众所周知的ImageJ程序的插件而开发,旨在解决分析荧光显微镜图像的挑战,其特征是低信噪比和异质背景。该算法有效地区分了信号和噪声像素,在消除噪声的同时保留了信号。该功能可以分析具有不同强度对象的图像,即使在低信噪比条件下也可以进行可靠的识别。此外,BGR提供了确定识别对象强度的能力,增强了该领域研究人员的实用性。本文介绍了算法和程序功能,并对其性能进行了测试。该程序可从网站https://kilianna.github.io/background-remover/免费下载。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Background Remover – An effective tool for processing noisy microscopy images

Background Remover – An effective tool for processing noisy microscopy images

Background Remover (BGR) is a novel software tool developed as a plugin to the well-known ImageJ program and designed to address the challenges of analysing fluorescent microscopy images characterised by low signal-to-noise ratios and heterogeneous backgrounds. The used algorithm effectively differentiates between signal and noise pixels, preserving the signal while eliminating noise. This functionality enables the analysis of images with objects of varying intensities, allowing for reliable identification even in low signal-to-noise ratio conditions. Furthermore, BGR offers the capability to determine the intensity of identified objects, enhancing its utility for researchers in the field. The paper describes the algorithm and the program functioning, as well as the carried out tests of its performance. The program is freely downloadable from the website https://kilianna.github.io/background-remover/

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来源期刊
Journal of microscopy
Journal of microscopy 工程技术-显微镜技术
CiteScore
4.30
自引率
5.00%
发文量
83
审稿时长
1 months
期刊介绍: The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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