Virtual dissection of Aedes aegypti mosquito using phase-contrast synchrotron microtomography

IF 1.9 4区 工程技术 Q3 MICROSCOPY
Gabriela Sena, Thaina Alvarenga, Ruan Ingliton Feio, Cícero Brasileiro Mello, Gabriel Fidalgo, Katrine Paiva, Tayane Tanure, Liebert Parreiras Nogueira, Marcos Vinícius Colaço, Arissa Pickler, Marcelo Salabert Gonzalez, Patricia Azambuja, Giuliana tromba, José Bento Pereira Lima, Ademir Xavier da Silva, Regina Cély Barroso
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Abstract

In this paper, in-line phase-contrast synchrotron microtomography was used to study the morphology of adult Aedes aegypti. These specimens are vectors of several arboviruses, causing dengue, chikungunya, Zika and yellow fever. The morphological details of this insect species are still incomplete and insufficient. To address this gap, this study examined whole specimens of Aedes aegypti in the adult phase at high resolution. For this, the adult samples were scanned in the microtomography beamline (SYRMEP) at the Italian Synchrotron Light Laboratory (ELETTRA). The phase-contrast technique allowed us to obtain high-quality images, which made it possible to evaluate the segmentation of structures on the rendered volume by the Dragonfly software. The combination of high-quality images and segmentation process provide adequate visualisation of different organs which could serve in assessing the effectiveness of innovative control population methods as a basis for future control studies of the insect vector.

Abstract Image

利用相衬同步加速器显微断层扫描技术对埃及伊蚊进行虚拟解剖。
本文采用在线相衬同步加速器显微断层扫描技术对埃及伊蚊成虫的形态进行了研究。这些标本是几种虫媒病毒的载体,可引起登革热、基孔肯雅热、寨卡和黄热病。这种昆虫的形态细节仍然不完整和不足。为了解决这一差距,本研究以高分辨率检查了埃及伊蚊成虫期的整个标本。为此,成年样品在意大利同步加速器光实验室(ELETTRA)的微断层扫描光束线(SYRMEP)中进行扫描。相衬技术使我们能够获得高质量的图像,这使得蜻蜓软件可以评估渲染体上的结构分割。高质量图像和分割处理的结合提供了不同器官的充分可视化,可用于评估创新控制种群方法的有效性,为今后昆虫媒介的控制研究奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of microscopy
Journal of microscopy 工程技术-显微镜技术
CiteScore
4.30
自引率
5.00%
发文量
83
审稿时长
1 months
期刊介绍: The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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