Journal of Neuroscience Methods最新文献

{"title":"Assessing the impact of transcranial electrical stimulation on intracranial and cerebral perfusion pressures in patients with severe craniocerebral injury: A novel methodological approach","authors":"Ailiang Ge,&nbsp;Jinan Bai,&nbsp;Yan Wang,&nbsp;Xin Li","doi":"10.1016/j.jneumeth.2025.110498","DOIUrl":"10.1016/j.jneumeth.2025.110498","url":null,"abstract":"<div><h3>Background</h3><div>Transcranial electrical stimulation (tES) protects brain cells and blood flow, making it an intriguing treatment option for skull and brain injury victims. Understanding how transcranial electroencephalography (tES) affects cerebral perfusion pressure (CPP) and intracranial pressure (ICP) might improve patient outcomes. Most techniques for evaluating ICP and CPP include intrusive procedures that might cause infections and hiccups. Additionally, current methods cannot optimize patient results by changing stimulation settings in real-time.</div></div><div><h3>New Method</h3><div>The Optimizing tES Parameters for Neuroprotection (O-tES-PN) approach addresses these issues. The system has many monitoring tools and an adaptive tES control mechanism. The O-tES-PN continually adjusts its dynamic stimulation settings based on intracranial and cerebral blood pressure. This keeps cerebral blood flow constant and reduces damage.</div></div><div><h3>Results</h3><div>Our findings show that stable ICP and CPP enhance CBF modulation with the O-tES-PN design. The device may modify settings in real-time to give more concentrated and effective treatments with fewer adverse effects.</div></div><div><h3>Comparison with Existing Methods</h3><div>Compared to invasive therapies, O-tES-PN is safer. It also improves therapy parameter control. Current technology allows continuous monitoring and stimulation level changes, which are limitations of previous systems.</div></div><div><h3>Conclusions</h3><div>The O-tES-PN treatment is less risky than other invasive treatments. Controlling the parameters of the treatment is also improved. The limitations of earlier systems are overcome by the current technology, which enables continuous monitoring and adjustments in stimulation level.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"422 ","pages":"Article 110498"},"PeriodicalIF":2.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using DeepLabCut-Live to probe state dependent neural circuits of behavior with closed-loop optogenetic stimulation","authors":"Melissa Gonzalez ,&nbsp;Mark A. Gradwell ,&nbsp;Joshua K. Thackray ,&nbsp;Kanaksha K. Temkar ,&nbsp;Komal R. Patel ,&nbsp;Victoria E. Abraira","doi":"10.1016/j.jneumeth.2025.110495","DOIUrl":"10.1016/j.jneumeth.2025.110495","url":null,"abstract":"<div><h3>Background</h3><div>Closed-loop behavior paradigms allow for real-time investigation of state-dependent neural circuits underlying behavior. However, studying context-dependent locomotor perturbations is challenging due to limitations in molecular tools and techniques for real-time manipulation of spinal circuits.</div></div><div><h3>New method</h3><div>We developed a novel closed-loop optogenetic stimulation paradigm that leverages DeepLabCut-Live pose estimation to manipulate primary sensory afferent activity at specific phases of the locomotor cycle in mice. A compact DeepLabCut model was trained to track hindlimb kinematics in real-time and integrated into the Bonsai visual programming framework. This system enabled LED triggered photo-stimulation of sensory neurons expressing channelrhodopsin based on user-defined pose-based criteria, such as stance or swing phase.</div></div><div><h3>Results</h3><div>Optogenetic activation of nociceptive TRPV1⁺ sensory neurons during treadmill locomotion reliably evoked paw withdrawal responses. Stimulation during the stance phase generated a brief withdrawal and impacted the duration of the following swing phase. Stimulation during the swing phase increased the height of paw withdrawal during swing and reduced the duration of the following stance phase.</div></div><div><h3>Comparison with existing methods</h3><div>This method allows for high spatiotemporal precision in manipulating spinal circuits based on locomotor phase. Unlike previous approaches, this closed-loop system accounts for state-dependent nature of sensorimotor responses, enabling controlled, real-time modulation of locomotion.</div></div><div><h3>Conclusions</h3><div>Integrating DeepLabCut-Live with optogenetics provides a powerful tool for dissecting the context-dependent role of sensory feedback and spinal interneurons in locomotion. This technique opens new avenues for uncovering the neural substrates of state-dependent behaviors and has broad applicability for studies of real-time closed-loop manipulation based on pose estimation.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"422 ","pages":"Article 110495"},"PeriodicalIF":2.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of somatosensory evoked potentials through fine intrafascicular stimulation via flexible carbon nanotube yarn electrodes under different isoflurane anaesthetics","authors":"Yapeng Zhang ,&nbsp;Shang Deng ,&nbsp;Muqiang Jian ,&nbsp;Xiaohua Zhang ,&nbsp;Yao Chen ,&nbsp;Jiguang Wang ,&nbsp;Xiaohong Sui","doi":"10.1016/j.jneumeth.2025.110496","DOIUrl":"10.1016/j.jneumeth.2025.110496","url":null,"abstract":"<div><h3>Background</h3><div>While most current studies have focused on the effects of transcutaneous and extrafascicular stimulation on SEPs, intrafascicular stimulation offers a more advantageous alternative. By providing closer access to target afferent fibers, it allows for lower stimulation currents and greater nerve fiber selectivity, making it a promising approach for achieving fine tactile feedback.</div></div><div><h3>New method</h3><div>This study employed intrafascicular stimulation with carbon nanotube yarn (CNTy) electrodes to explore its impact on tactile feedback under different levels of isoflurane anaesthesia in rats. Bipolar and monopolar stimulation were applied to stimulate tibial nerves and the SEPs were recorded to analyse quantitatively. <em>Results:</em> The stimulation current threshold increased with increasing anaesthetic concentration for both bipolar and monopolar stimulation for each rat. As the anaesthesia concentration increased, the amplitude progressively decreased (P &lt; 0.001, 1.0 % vs 2.0 %, monopolar; P &lt; 0.01, 1.0 % vs 2.0 %, bipolar), the latency was prolonged (P &lt; 0.01, 1.0 % vs 2.0 %, monopolar; P &lt; 0.05, 1.0 % vs 2.0 %, bipolar), and the spectral power of early and late components significantly decreased (P &lt; 0.01, 1.0 % vs 2.0 %, monopolar, P_early; P &lt; 0.05, 1.0 % vs 2.0 %, monopolar, P_late). <em>Comparison with existing methods:</em> For the first time, this study utilized CNTy electrodes via intrafascicular stimulation to investigate the characteristics of SEPs under varying isoflurane concentrations. <em>Conclusions:</em> This study provides a solid foundation for quantifying tactile feedback of intrafascicular stimulation during rat experiments, and reveals among the three anesthetic concentrations tested, 1.0 % exerted the minimal impact on all measured SEP characteristics in rats.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110496"},"PeriodicalIF":2.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144137705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability and suitability of rat pup organotypic spinal cord slice cultures in a transection injury model","authors":"Ciara Shortiss ,&nbsp;Linda Howard ,&nbsp;Siobhan S. McMahon","doi":"10.1016/j.jneumeth.2025.110499","DOIUrl":"10.1016/j.jneumeth.2025.110499","url":null,"abstract":"<div><h3>Background</h3><div><em>Ex vivo</em> organotypic spinal cord slice culture (OSC) models are advantageous for spinal cord injury (SCI) research. They retain <em>in vivo</em> cellular interactions but involve fewer ethical concerns and are easier to manipulate than <em>in vivo</em> models. Due to improved viability OSCs derived from early postnatal animals are preferred. A transection injury OSC model derived from postnatal day (P) 4 rats has been validated, however as rodent spinal cord development changes significantly in early postnatal weeks, older postnatal day animals may be more appropriate for OSC models.</div></div><div><h3>New method</h3><div>This study compared the viability of OSCs derived from P11 rats with the established P4 transection model.</div></div><div><h3>Results</h3><div>No significant differences in cell viability were found between P4 and P11 control slices, or between control and injured slices within each age group. P11 OSCs also demonstrated strong viable cell ingrowth into a transection injury gap post-injury, comparable to P4 OSCs, and increased their volume fraction of immunostaining for the glial scar markers GFAP and CSPGs along transection gap edges. Sex was not found to affect any of these parameters.</div></div><div><h3>Comparison with existing methods</h3><div>Our findings demonstrate that P11 OSCs maintain viability and cellular ingrowth post transection injury comparable to that of the well-established P4 derived OSCs. P11 OSCs were also found to exhibit a glial scarring response post transection injury.</div></div><div><h3>Conclusions</h3><div>OSCs from P11 animals maintain viability comparable to P4 OSCs while also exhibiting a scarring response. This study concludes that P11 OSCs are a valid alternate to P4 OSC transection SCI model.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110499"},"PeriodicalIF":2.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain MRIs classification based on 2D SWD-MF-DFA","authors":"Jing Wang ,&nbsp;Xinpei Wu ,&nbsp;Haozhe Wang ,&nbsp;Jian Wang","doi":"10.1016/j.jneumeth.2025.110468","DOIUrl":"10.1016/j.jneumeth.2025.110468","url":null,"abstract":"<div><h3>Background:</h3><div>To improve imaging classification accuracy, we modify the traditional 2D multifractal trend fluctuation analysis (MF-DFA) method to better preserve local feature values. Inspired by MF-DFA, we develop a novel method for extracting eigenvalues, enhancing the precision of imaging analysis.</div></div><div><h3>New method:</h3><div>In this paper, we propose an enhanced algorithm building upon the traditional 2D MF-DFA. Our approach introduces a 2D sliding window (SWD) technique for feature value extraction. Initially, the local generalized Hurst index of the imaging is derived using the SWD algorithm, based on MF-DFA principles. Subsequently, the generalized Hurst index is recalculated for the digital matrix formed by these local Hurst indexes. These vectors are then input into a support vector machine (SVM) for classification. This methodology seeks to refine the traditional 2D MF-DFA by more effectively preserving local feature values in imaging.</div></div><div><h3>Results:</h3><div>The classification accuracy of the SWD eigenvalue extraction method based on 2D MF-DFA reaches 91.54%.</div></div><div><h3>Comparison with existing methods:</h3><div>We employ brain magnetic resonance imaging (MRI) data sets to evaluate the efficacy of both the conventional 2D MF-DFA method and our proposed feature value extraction technique. Both methods are applied alongside a SVM for classification. The findings reveal that the conventional 2D MF-DFA method yields a classification accuracy of 59.40%, while our SWD feature value extraction method attains a classification accuracy of 91.54%.</div></div><div><h3>Conclusion:</h3><div>This substantial performance enhancement underscores the superiority of the SWD approach over the conventional method.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110468"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying TMS-induced sleep changes: A novel neurophysiological assessment approach","authors":"Xiqian Qi ,&nbsp;Nanhua Zhou ,&nbsp;Daibo Zheng","doi":"10.1016/j.jneumeth.2025.110485","DOIUrl":"10.1016/j.jneumeth.2025.110485","url":null,"abstract":"<div><h3>Background</h3><div>Neuromodulation methods such as transcranial magnetic stimulation (TMS) show promise for the research and manipulation of brain activity during sleep. Nevertheless, when it comes to accurately documenting changes in neurophysiology occurring in real-time, typical sleep evaluation approaches, such as subjective reports and routine polysomnography, fall short. Accurately quantifying changes in sleep architecture and cortical excitability generated by TMS is challenging due to these constraints.</div></div><div><h3>New method</h3><div>A framework for electroencephalogram spectral analysis (EEG-SA) is presented in this paper to tackle these issues. This method compares pre- and post-TMS spectral power fluctuations in the important frequency bands delta, theta, alpha, beta, and gamma. EEG-SA provides a comprehensive evaluation of neurophysiology to identify changes in spectral properties and dynamic shifts in cortical excitability caused by TMS.</div></div><div><h3>Results</h3><div>Research shows that EEG-SA can detect changes in sleep architecture caused by TMS. In particular, improvements in slow-wave activity and cortical synchronization are analyzed, two factors crucial to better sleep quality. These findings highlight the promise of EEG-SA for improving methods of sleep regulation.</div></div><div><h3>Comparison with existing methods</h3><div>A precise, real-time measurement of neurophysiological changes is provided by EEG-SA, in contrast to conventional evaluation approaches that depend on subjective sleep reports and routine polysomnography. This paradigm analyzes TMS-induced sleep changes better than previous methods by providing a more objective and thorough evaluation.</div></div><div><h3>Conclusion</h3><div>TMS-induced sleep alterations may be reliably quantified using EEG-SA, opening the door to individualized therapy for sleep disorders. Insomnia, hypersomnia, and other sleep disorders might benefit from treatment tactics that optimize TMS settings according to individual neurophysiological responses. One way to improve the efficacy of sleep modulation treatments is to include EEG-SA in clinical settings.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110485"},"PeriodicalIF":2.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating safe infrared neural stimulation parameters: Calcium dynamics and excitotoxicity thresholds in dorsal root ganglia neurons","authors":"Parinaz Abdollahian ,&nbsp;Kunyang Sui ,&nbsp;Guanghui Li ,&nbsp;Rune W. Berg ,&nbsp;Marcello Meneghetti ,&nbsp;Christos Markos","doi":"10.1016/j.jneumeth.2025.110484","DOIUrl":"10.1016/j.jneumeth.2025.110484","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;As a promising neural stimulation technique, infrared neural stimulation (INS) has recently gained significant attention due to its ability to stimulate neuronal activities without needing exogenous agents. NIR light is absorbed by water of the tissue producing local thermal effects. Therefore, INS is a suitable candidate for localized and targeted neural stimulation. However, despite the wide variety of research studies on INS applications, limited studies have focused on identifying and optimizing the stimulation parameters to avoid potential excitotoxicity. This study evaluates the dorsal root ganglia (DRG) neurons' response under INS with varying intensities and illumination time.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;New method&lt;/h3&gt;&lt;div&gt;Here, DRG neurons are cultured and labeled by the CamkII-GCaMP6s virus. The neurons were exposed to infrared laser pulses (2.01 µm wavelength, different powers of 2.5 mW, 5 mW, 7.5 mW, and 10 mW) for durations of 300 s and 400 s. The light was delivered through a silica optical fiber aligned and stabilized within a free-space optical setup. Simultaneous with INS, neuronal activity was evaluated by calcium imaging through a fluorescence microscope. This method allowed real-time monitoring of neuronal calcium dynamics under different stimulation conditions, preparing an overview of the safe thresholds for INS.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;It was found that calcium saturation has happened for the neurons in exposure to light intensities (7.5 mW and 10 mW) for 300 s, representing potential excitotoxicity. In contrast, with the same exposure time, lower light intensities (2.5 mW and 5 mW) did not show significant signs of calcium saturation or neuronal damage. Moreover, in some neuronal networks, the peripheral neurons of the illuminated area revealed indirect activation, indicating inter-neuronal communication effects.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Comparison with existing methods&lt;/h3&gt;&lt;div&gt;Compared to previous studies that have explored the use of INS on DRG neurons, our work introduces a systematic approach to evaluate the light intensity-dependent INS, while addressing the critical issue of potential thermal injury. While earlier research has demonstrated the ability of INS to modulate neuronal activity and reduce electrical artifacts in electrophysiological recordings, concerns regarding excitotoxicity and neuronal damage remain insufficiently investigated. We examined a range of laser intensities (2.5 mW to 10 mW) to determine the safe exposure thresholds and optimize the photothermal impact. Furthermore, by utilizing CamKII-GCaMP6s virus-modified neurons, we enhance sensitivity in detecting calcium influx, providing a more precise evaluation of neuronal responses to INS. Therefore, here, we provide the knowledge for safe INS.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;This work identifies the required laser stimulation parameters, particularly intensity and illumination time of the tissue for efficien","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110484"},"PeriodicalIF":2.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aseptic, semi-sealed cranial chamber implants for chronic multi-channel neurochemical and electrophysiological neural recording in nonhuman primates","authors":"Jiwon Choi ,&nbsp;Usamma Amjad ,&nbsp;Raymond Murray ,&nbsp;Ritesh Shrivastav ,&nbsp;Tobias Teichert ,&nbsp;Baldwin Goodell ,&nbsp;Matthew Olson ,&nbsp;David J. Schaeffer ,&nbsp;Julia K. Oluoch ,&nbsp;Helen N. Schwerdt","doi":"10.1016/j.jneumeth.2025.110467","DOIUrl":"10.1016/j.jneumeth.2025.110467","url":null,"abstract":"<div><h3>Background</h3><div>Invasive electrophysiological recordings in subcortical structures of nonhuman primates typically involve implanting electrodes into the brain through a skull-mounted chamber. These electrodes may be attached to the chamber temporarily for hours of neural recording, or permanently for long-term studies. Current challenges involve maintaining asepsis and integrating dual-modality monitoring of both electrical and chemical neural activity.</div></div><div><h3>New method</h3><div>We developed an implantable neural interface that provides such dual-modality monitoring in monkeys, while maintaining aseptic conditions for year-long periods. We leveraged osseointegrating materials and hermetic sealing strategies to prevent the transmission of pathogenic species, while preserving the modular functionality of chamber systems, such as sensor depth adjustability. The system also features an aspirating port for culturing chamber fluid to ensure continued asepsis.</div></div><div><h3>Results</h3><div>Our chamber system was shown to provide successful recordings of dopamine and electrical neural activity in two monkeys while maintaining negative bacteria culture results for over a year post-implantation.</div></div><div><h3>Comparison with existing methods</h3><div>Sealed chamber systems prevent contamination and reduce the risk of compromising animal health by minimizing the accumulation of pathogenic organisms. Such sealed chambers also eliminate the need for frequent cleaning. However, neurochemical measurements require specialized electrodes with fragile carbon fiber tips and are not compatible with recently developed, sealed chamber systems.</div></div><div><h3>Conclusion</h3><div>This advanced chamber design builds upon traditional chamber protocols to enable chronic measurements of chemical and electrical neural activity. This approach facilitates novel ways to study the brain in behaving primates while prioritizing the long-term health and welfare of the animals.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110467"},"PeriodicalIF":2.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in endovascular brain computer interface: Systematic review and future implications","authors":"Julien Ognard ,&nbsp;Gerard El Hajj ,&nbsp;Onam Verma ,&nbsp;Sherief Ghozy ,&nbsp;Ramanathan Kadirvel ,&nbsp;David F. Kallmes ,&nbsp;Waleed Brinjikji","doi":"10.1016/j.jneumeth.2025.110471","DOIUrl":"10.1016/j.jneumeth.2025.110471","url":null,"abstract":"<div><h3>Background</h3><div>Brain-computer interfaces (BCIs) translate neural activity into real-world commands. While traditional invasive BCIs necessitate craniotomy, endovascular BCIs offer a minimally invasive alternative using the venous system for electrode placement.</div></div><div><h3>New method</h3><div>This systematic review evaluates the technical feasibility, safety, and clinical outcomes of endovascular BCIs, discussing their future implications. A systematic review was conducted per PRISMA guidelines. The search spanned PubMed, Web of Science, and Scopus databases using keywords related to neural interfaces and endovascular approaches. Studies were included if they reported on endovascular BCIs in preclinical or clinical settings. Dual independent screening and extraction focused on electrode material, recording capabilities, safety parameters, and clinical efficacy.</div></div><div><h3>Results</h3><div>From 1385 initial publications, 26 met the inclusion criteria. Seventeen studies investigated the Stentrode device. Among the 24 preclinical studies, 16 used ovine or rodent models, and 9 addressed engineering or simulation aspects. Two clinical studies reported six ALS patients successfully using an endovascular BCI for digital communication. Preclinical data established the endovascular ovine model, demonstrating stable neural recordings and vascular changes with long-term implantation. Key challenges include thrombosis risk, long-term electrode stability, and anatomical variability.</div></div><div><h3>Comparison with existing methods</h3><div>Endovascular BCI reduced invasiveness, improved safety profiles, with comparable neural recording fidelity to invasive methods, and promising preliminary clinical outcomes in severely paralyzed patients.</div></div><div><h3>Conclusions</h3><div>Early results are promising, but clinical data remain scarce. Further research is needed to optimize signal processing, enhance electrode biocompatibility, and refine endovascular procedures for broader clinical applications.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110471"},"PeriodicalIF":2.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spike detection of human sympathetic nerve activity using wavelet transformation and Valsalva maneuver denoising","authors":"Surat Kulapatana ,&nbsp;Stefano Rigo ,&nbsp;Vasile Urechie ,&nbsp;Robert J. Brychta ,&nbsp;Raffaello Furlan ,&nbsp;Italo Biaggioni ,&nbsp;André Diedrich","doi":"10.1016/j.jneumeth.2025.110482","DOIUrl":"10.1016/j.jneumeth.2025.110482","url":null,"abstract":"<div><h3>Background</h3><div>Sympathetic function is directly assessed by microneurography measuring muscle sympathetic nerve activity (MSNA). The recordings are typically corrupted with noise and require denoising. We aim to estimate microneurographic noise individually from physiologically suppressed MSNA during Valsalva phase 4 (VM4).</div></div><div><h3>New method</h3><div>We developed MSNA adaptive processing (MAP). MSNA recordings during Valsalva were transformed by stationary wavelet transformation. Level-specific noise thresholds were computed from 4 SD of detail coefficients from VM4 and were implemented for denoising. The denoised signals were inverse transformed, then the MSNA spikes were detected.</div><div>We compared detection performance of the MAP with the current two-stage kurtosis method in simulated MSNA signals, and recordings from 17 healthy and 19 postural orthostatic tachycardia syndrome (POTS) female subjects performing Valsalva.</div></div><div><h3>Results</h3><div>The MAP had higher correct detections of MSNA spikes than the kurtosis method in simulated signals wit high burst rate (50 burst/min) and low signal-to-noise ratio (SNR =2) (MAP vs kurtosis; 23.81 ± 15.49 % vs 16.98 ± 12.75 %, p &lt; 0.001). The improvement was confirmed by shorter error distance of the precision-recall plot (0.535 ± 0.175 vs 0.542 ± 0.177, p = 0.011).</div><div>The MAP detected higher spike rate during VM phase 2 in healthy (24.11 ± 9.85 vs 19.57 ± 8.60 spike/s, p = 0.049), but non-significant in POTS (24.19 ± 13.70 vs 20.30 ± 11.85 spike/s, p = 0.101).</div></div><div><h3>Comparison with existing methods</h3><div>The detection performance of the MAP is superior to the current two-stage kurtosis method.</div></div><div><h3>Conclusions</h3><div>The proposed MAP method individually estimating noise from VM4 could improve MSNA spike detection, compared with the kurtosis method. The advantages are most prominent in high burst rate and low SNR MSNA recordings.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110482"},"PeriodicalIF":2.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}