Journal of Liposome Research最新文献_第4页

Transdermal application of diacerin loaded-terpene enriched invasomes: an approach to augment anti-edema and nociception inhibition activity. 透皮应用富含二碳酸酯的萜类侵袭体：一种增强抗水肿和抑制痛觉活性的方法。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2025-03-01 Epub Date: 2024-07-29 DOI: 10.1080/08982104.2024.2382974

Sadek Ahmed, Michael M Farag, Mohamed A Sadek, Diana E Aziz

{"title":"Transdermal application of diacerin loaded-terpene enriched invasomes: an approach to augment anti-edema and nociception inhibition activity.","authors":"Sadek Ahmed, Michael M Farag, Mohamed A Sadek, Diana E Aziz","doi":"10.1080/08982104.2024.2382974","DOIUrl":"10.1080/08982104.2024.2382974","url":null,"abstract":"This study aimed to formulate diacerein loaded terpene-enriched invasomes (DCN-TINV) to fulfill a fruitful management of osteoarthritis. A 23 factorial design was adopted, including A: cholesterol concentration (%w/v), B: ethanol volume (mL) and C: phosphatidylcholine: drug ratio as the studied factors. Invasomes were constructed using the thin film hydration technique. Herein, percent entrapment efficiency (EE%), particle size (PS), poly-dispersity index (PDI) and zeta potential (ZP) were statistically analyzed using Design-Expert® software to select the optimum formula. The selected criteria for detecting the optimum formula were restricting PS (<350 nm), dismissing PDI, magnifying ZP (as absolute value) and EE%. The selected formula was further scrutinized through multiple in-vitro studies, including Fourier-transform infrared spectroscopy, differential scanning calorimetry, pH measurement, stability study, release profile and transmission electron microscopy. Furthermore, the ex-vivo performance was evaluated through ex-vivo skin permeation and deposition. Finally, it was subjected to an array of in-vivo tests, namely Draize test, histopathology, In-vivo skin penetration, edema size, and nociception inhibition measurements. The optimum formula with desirability (0.913) demonstrated EE% (89.21% ± 2.12%), PS (319.75 ± 10.11 nm), ZP (-55 ± 3.96 mV) and a prolonged release profile. Intriguingly, revamped skin permeation (1143 ± 32.11 µg/cm2), nociception inhibition (77%) and In-vivo skin penetration (144 µm) compared to DCN suspension (285 ± 21.25 µg/cm2, 26% and 48 µm, respectively) were displayed. The optimum DCN-TINV exhibited plausible safety and stability profiles consolidated with auspicious efficacy for better management of osteoarthritis.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-14"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of size and pH-sensitivity of liposomes on cellular uptake pathways and pharmacokinetics of encapsulated gemcitabine. 脂质体的大小和 pH 敏感性对包裹吉西他滨的细胞摄取途径和药代动力学的影响

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2025-03-01 Epub Date: 2024-08-09 DOI: 10.1080/08982104.2024.2389969

Mingtan Tang, Sasi Bhushan Yarragudi, Patrick Pan, Kaiyun Yang, Manju Kanamala, Zimei Wu

{"title":"Effect of size and pH-sensitivity of liposomes on cellular uptake pathways and pharmacokinetics of encapsulated gemcitabine.","authors":"Mingtan Tang, Sasi Bhushan Yarragudi, Patrick Pan, Kaiyun Yang, Manju Kanamala, Zimei Wu","doi":"10.1080/08982104.2024.2389969","DOIUrl":"10.1080/08982104.2024.2389969","url":null,"abstract":"To enhance cytoplasmic delivery efficiency, pH-sensitive liposomes (PSL) have been proposed as a novel strategy. To facilitate clinical translation, this study aims to understand the impact of both size and pH-sensitivity on cellular uptake pathways, intracellular trafficking and pharmacokinetics of liposomes. The large liposomes (130-160 nm) were prepared using thin-film hydration method, while small liposomes (∼60 nm) were fabricated using microfluidics, for both PSL and non-pH-sensitive liposomes (NPSL). Cellular uptake pathways and intracellular trafficking was investigated through confocal imaging with aid of various endocytosis inhibitors. Intracellular gemcitabine delivery by various liposomal formulations was quantified using HPLC, and the cytotoxicity was assessed via cell viability assays. Pharmacokinetics of gemcitabine loaded in various liposomes was evaluated in rats following intravenous administration. Larger liposomes had a higher loading capacity for hydrophilic gemcitabine (7% vs 4%). Small PSL exhibited superior cellular uptake compared to large PSL or NPSLs. Moreover, the alkalization of endosomes significantly attenuated the cellular uptake of PSL. Large liposomes (PSL and NPSL) predominantly entered cells via clathrin-dependent pathway, whereas small liposomes partially utilized caveolae-dependent pathway. However, the long circulation of the liposomes, as measured by the encapsulated gemcitabine, was compromised by both pH-sensitivity and size reduction (9.5 h vs 5.3 h). Despite this drawback, our results indicate that small PSL holds promise as vectors for the next generation of liposomal nanomedicine, owing to their superior cytoplasmic delivery efficiency.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"44-54"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dyeing of polyacrylonitrile knitted fabric using liposomes. 使用脂质体对聚丙烯腈针织物进行染色。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2025-03-01 Epub Date: 2024-08-26 DOI: 10.1080/08982104.2024.2396107

Rıza Atav, Selin Güler Köylüoğlu, Aminoddin Haji, Uğur Ergünay

{"title":"Dyeing of polyacrylonitrile knitted fabric using liposomes.","authors":"Rıza Atav, Selin Güler Köylüoğlu, Aminoddin Haji, Uğur Ergünay","doi":"10.1080/08982104.2024.2396107","DOIUrl":"10.1080/08982104.2024.2396107","url":null,"abstract":"In this study, it was aimed to analyze the effects of liposomes on the dyeing of polyacrylonitrile fabrics. For this purpose, firstly liposome synthesis was carried out, and then liposome production was confirmed by Fourier transform infrared spectroscopy analysis. Additionally, zeta potential measurements were carried out to see whether stable structures were formed. Then, a selected basic dye was encapsulated with a liposome and the possibilities of using these capsules as alternative to retarders in the dyeing of polyacrylonitrile fabrics were examined. According to results obtained, it can be said that the 1% solution of synthesized liposomes creates a more stable suspension with a polydispersity index of 0.472 and the average particle size of 165.2 nm. On the other hand, it has been revealed that if 1% liposome is used in dyeing, a kind of retarder effect can be achieved in the dyeing of polyacrylonitrile fabrics. Moreover, it can be said that the decrease in color efficiency, that is, the loss of yield, caused by the use of liposome at the end of dyeing is lower compared to the retarder. This is also a very important issue, because a good retarder is expected to slow down the dye uptake, but not reduce the dye intake too much at the end of the dyeing. Dyeing levelness (%) was found to be 96.1, 97.4, and 97.1 for dyeings without auxiliary, with 1% cationic retarder and with 1% liposome, respectively. Beyond this, no significant difference was observed in terms of fastness of dyeing.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"55-63"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, optimization, characterization, and in vitro evaluation of metformin-loaded liposomes for triple negative breast cancer treatment. 用于治疗三阴性乳腺癌的二甲双胍脂质体的设计、优化、表征和体外评估。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-03-08 DOI: 10.1080/08982104.2024.2321528

Daiva Vozgirdaite, Katel Hervé-Aubert, Rustem Uzbekov, Igor Chourpa, Emilie Allard-Vannier

{"title":"Design, optimization, characterization, and in vitro evaluation of metformin-loaded liposomes for triple negative breast cancer treatment.","authors":"Daiva Vozgirdaite, Katel Hervé-Aubert, Rustem Uzbekov, Igor Chourpa, Emilie Allard-Vannier","doi":"10.1080/08982104.2024.2321528","DOIUrl":"10.1080/08982104.2024.2321528","url":null,"abstract":"Recently, metformin (Met) has shown to have antineoplastic properties in cancer treatment by improving hypoxic tumor conditions, and causing reduction in the synthesis of biomolecules, which are vital for cancer growth. However, as an orally administered drug, Met has low bioavailability and rapid renal clearance. Thus, the goal of this study was to vectorize Met inside liposomes in the context of triple negative breast cancer (TNBC), which currently lacks treatment options when compared to other types of breast cancer. Vectorization of Met inside liposomes was done using Bangham method by implementing double design of experiment methodology to increase Met drug loading (minimum-run resolution V characterization design and Box-Behnken design), as it is generally extremely low for hydrophilic molecules. Optimization of Met-loaded liposome synthesis was successfully achieved with drug loading of 190 mg/g (19% w/w). The optimal Met-liposomes were 170 nm in diameter with low PdI (< 0.1) and negative surface charge (-20 mV), exhibiting sustained Met release at pH 7.4. The liposomal Met delivery system was stable over several months, and successfully reduced TNBC cell proliferation due to the encapsulated drug. This study is one the first reports addressing liposome formulation through thin-film hydration using two design of experiment methods aiming to increase drug loading of Met.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"547-561"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140065225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposomal propranolol for treatment of infantile hemangioma at compounding pharmacies. 复方药房用于治疗婴儿血管瘤的普萘洛尔脂质体。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-02-09 DOI: 10.1080/08982104.2024.2313452

Antigone Nifli, Aggeliki Liakopoulou, Elena Mourelatou, Konstantinos Avgoustakis, Sophia Hatziantoniou

{"title":"Liposomal propranolol for treatment of infantile hemangioma at compounding pharmacies.","authors":"Antigone Nifli, Aggeliki Liakopoulou, Elena Mourelatou, Konstantinos Avgoustakis, Sophia Hatziantoniou","doi":"10.1080/08982104.2024.2313452","DOIUrl":"10.1080/08982104.2024.2313452","url":null,"abstract":"Infantile hemangiomas (IH) are common benign soft tissue tumors, frequently affecting infants. While Propranolol Hydrochloride (Pro HCl) has emerged as a promising treatment for IH, its topical application remains challenging due to the need for stable and efficacious carriers. This study investigates the potential of preformulated liposomes as carriers for topical delivery of Pro HCl for the treatment of IH in compounding pharmacies. Liposomes loaded with Pro HCl were prepared using active pharmaceutical ingredient or commercially available propranolol tablets and various dilution media, including Water for Injection (WFI), Dextrose 5%, and NaCl 0.9%. The physicochemical properties of the liposomal formulations (Pro HCl content, encapsulation efficiency, loading capacity, and colloidal stability) were assessed over a 90-day storage at 4 °C. In vitro release kinetics and transdermal permeation of Pro HCl from liposomes were also evaluated. Liposome properties were influenced by the dilution medium. Pro HCl content remained stable in liposomes encapsulating API (Lipo-Pro), regardless of the dilution medium. Lipo-Pro showed sustained drug release over time, suggesting its potential for maintaining therapeutic levels. Pro HCl exhibited enhanced transdermal permeability from Lipo-Pro compared to aqueous solution, indicating its potential for topical IH treatment. Preformulated liposomes offer a stable and effective carrier for Pro HCl, potentially suitable for extemporaneous preparations in compounding pharmacies. Their enhanced transdermal permeability presents a promising alternative for topical IH treatment. This study provides valuable insights into the development of innovative and effective drug delivery strategies for managing IH, with future research focusing on in vivo applications and therapeutic potential.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"523-534"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethosomes as a carrier for transdermal drug delivery system: methodology and recent developments. 乙硫体作为透皮给药系统的载体：方法和最新进展。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-04-27 DOI: 10.1080/08982104.2024.2339896

Karishma Mahajan, Poonam Sharma, Vikrant Abbot, Kalpana Chauhan

{"title":"Ethosomes as a carrier for transdermal drug delivery system: methodology and recent developments.","authors":"Karishma Mahajan, Poonam Sharma, Vikrant Abbot, Kalpana Chauhan","doi":"10.1080/08982104.2024.2339896","DOIUrl":"10.1080/08982104.2024.2339896","url":null,"abstract":"Transdermal drug delivery systems (TDDS) have received significant attention in recent years. TDDS are flexible systems that transport active components to the skin for either localized or systemic delivery of drugs through the skin. Among the three main layers of skin, the outermost layer, called the stratum corneum (SC), prevents the entry of water-loving bacteria and drugs with a high molecular weight. The challenge lies in successfully delivering drugs through the skin, which crosses the stratum corneum. The popularity of lipid-based vesicular delivery systems has increased in recent years due to their ability to deliver both hydrophilic and hydrophobic drugs. Ethosomes are specialized vesicles made of phospholipids that can store large amounts of ethanol. Ethosome structure and substance promote skin permeability and bioavailability. This article covers ethosome compositions, types, medication delivery techniques, stability, and safety. In addition to this, an in-depth analysis of the employment of ethosomes in drug delivery applications for a wide range of diseases has also been discussed. This review article highlights different aspects of ethosomes, such as their synthesis, characterization, marketed formulation, recent advancements in TDDS, and applications.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"697-714"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140858561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronic acid-coated liposomes for enhanced in vivo efficacy of neogambogic acid via active tumor cell targeting and prolonged systemic exposure. 透明质酸包裹的脂质体可通过肿瘤细胞靶向和延长全身暴露时间来增强新甘草酸的体内疗效。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-05-11 DOI: 10.1080/08982104.2024.2348643

Hongzhen Lv, Miao Miao, Zhichao Wu, Cheng Huang, Xiaozhu Tang, Rugen Yan

{"title":"Hyaluronic acid-coated liposomes for enhanced in vivo efficacy of neogambogic acid via active tumor cell targeting and prolonged systemic exposure.","authors":"Hongzhen Lv, Miao Miao, Zhichao Wu, Cheng Huang, Xiaozhu Tang, Rugen Yan","doi":"10.1080/08982104.2024.2348643","DOIUrl":"10.1080/08982104.2024.2348643","url":null,"abstract":"Neogambogic acid (NGA), which possesses a variety of anticancer activities, is visualized as an anticancer bioactive ingredient. However, the huge vascular stimulation, poor aqueous solubility, and short half-life restricted its clinical use. In this work, an effective nanocarrier was explored to reduce toxicity and enhance the tumor-targeted delivery. Two liposomal formulations, neogambogic acid liposomes (NGA-L), and hyaluronic acid-coated neogambogic acid liposomes (HA-NGA-L) were prepared and characterized with high encapsulation efficiency, slow pattern of drug release, narrow size distribution and higher stability. The cytotoxicity and cellular uptake of HA-NGA-L were higher than those of NGA-L in MDA-MB-231 cells (high CD44 expression), while no obvious differences in MCF-7 cells with (low CD44 expression), suggesting the CD44-mediated cellular internalization of hyaluronic acid-modified liposomes enhanced the cytotoxicity. Mechanistically, elevation of Bax and caspase-3 as well as downregulation of Bcl-2 led to cell apoptosis. Besides, the vascular stimulation and the hemolysis test indicated good safety of HA-NGA-L. In addition, HA-NGA-L was the effective nanocarrier to repress tumor proliferation in MDA-MB-231 tumor xenograft mouse through CD44 mediated active targeting without any obvious histopathological abnormities on major organs. Immunohistochemistry analysis revealed the enhanced elevation of Bax and caspase-3, and reduced expression of Bcl-2 contribute to apoptosis in tumors. Meanwhile, HA-NGA-L increased the AUC and t1/2 by 5.34-fold and 3.94-fold, respectively. In summary, the present study shows that HA-NGA-L may be safe and effective for the tumor-targeted delivery of neogambogic acid.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"605-616"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymeric liposomes of emtricitabine employing modified pullulan-an attempt to reduce associated hepatotoxicity. 采用改性拉普兰的恩曲他滨聚合脂质体--减少相关肝毒性的尝试。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-07-01 DOI: 10.1080/08982104.2024.2362352

Sayani Bhattacharyya, Lahari R, Ranganath Mk

{"title":"Polymeric liposomes of emtricitabine employing modified pullulan-an attempt to reduce associated hepatotoxicity.","authors":"Sayani Bhattacharyya, Lahari R, Ranganath Mk","doi":"10.1080/08982104.2024.2362352","DOIUrl":"10.1080/08982104.2024.2362352","url":null,"abstract":"Emtricitabine (FTC) a BCS class I drug, is used for HIV prevention. The high solubility of the drug is the leading cause of severe hepatotoxicity and lactic acidosis. This research focuses on the use of modified pullulan for the preparation of polymeric liposomes of FTC. Modified pullulan was synthesized using cholesterol, and succinic anhydride in a controlled chemical environment. The formation of the polymer was established through analysis of spectra. Varying the drug-polymer ratio (1:1, 1:2, and 1:3), the drug-polymer composite was loaded in the vesicular system of soya phosphatidylcholine and cholesterol. Formulations were evaluated for drug entrapment, particle size, surface morphology, and in vitro and ex vivo drug release. An in vivo study of the pure drug and the best formulation on mice was conducted for 28 days following daily oral administration to evaluate the effect on liver and hematological parameters. The best formulation was further subjected to cytotoxicity study on hepatic cell lines. Spectral analysis confirmed the formation of modified pullulan. All formulations showed high drug entrapment in the nanovesicles. The in vitro and ex vivo drug release profiles depicted a controlled release of the drug. Hematological parameters were found to be under control in the animals throughout the experimentation. A comparative histopathology study on the livers and cytotoxicity study on hepatic cell lines revealed the safety of the best formulation over the pure drug. Hence it can be concluded that polymeric liposomes of FTC can be a promising mode of delivery to overcome its limitations.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"630-639"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposomal nano-carriers mediated targeting of liver disorders: mechanisms and applications. 纳米脂质体载体介导的肝脏疾病靶向治疗：机制与应用。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-07-10 DOI: 10.1080/08982104.2024.2377085

Mona M AbouSamra

引用次数: 0

Targeting of M2 macrophages with IL-13-functionalized liposomal prednisolone inhibits melanoma angiogenesis in vivo. 用 IL-13 功能化脂质体泼尼松龙靶向 M2 巨噬细胞可抑制体内黑色素瘤血管生成。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-02-20 DOI: 10.1080/08982104.2024.2315452

Alina Sesarman, Lavinia Luput, Valentin-Florian Rauca, Laura Patras, Emilia Licarete, Marta-Szilvia Meszaros, Bogdan Razvan Dume, Giorgiana Negrea, Vlad-Alexandru Toma, Dana Muntean, Alina Porfire, Manuela Banciu

{"title":"Targeting of M2 macrophages with IL-13-functionalized liposomal prednisolone inhibits melanoma angiogenesis in vivo.","authors":"Alina Sesarman, Lavinia Luput, Valentin-Florian Rauca, Laura Patras, Emilia Licarete, Marta-Szilvia Meszaros, Bogdan Razvan Dume, Giorgiana Negrea, Vlad-Alexandru Toma, Dana Muntean, Alina Porfire, Manuela Banciu","doi":"10.1080/08982104.2024.2315452","DOIUrl":"10.1080/08982104.2024.2315452","url":null,"abstract":"The intricate cooperation between cancer cells and nontumor stromal cells within melanoma microenvironment (MME) enables tumor progression and metastasis. We previously demonstrated that the interplay between tumor-associated macrophages (TAMs) and melanoma cells can be disrupted by using long-circulating liposomes (LCLs) encapsulating prednisolone phosphate (PLP) (LCL-PLP) that inhibited tumor angiogenesis coordinated by TAMs. In this study, our goal was to improve LCL specificity for protumor macrophages (M2-like (i.e., TAMs) macrophages) and to induce a more precise accumulation at tumor site by loading PLP into IL-13-conjugated liposomes (IL-13-LCL-PLP), since IL-13 receptor is overexpressed in this type of macrophages. The IL-13-LCL-PLP liposomal formulation was obtained by covalent attachment of thiolated IL-13 to maleimide-functionalized LCL-PLP. C57BL/6 mice bearing B16.F10 s.c melanoma tumors were used to investigate the antitumor action of LCL-PLP and IL-13-LCL-PLP. Our results showed that IL-13-LCL-PLP formulation remained stable in biological fluids after 24h and it was preferentially taken up by M2 polarized macrophages. IL-13-LCL-PLP induced strong tumor growth inhibition compared to nonfunctionalized LCL-PLP at the same dose, by altering TAMs-mediated angiogenesis and oxidative stress, limiting resistance to apoptosis and invasive features in MME. These findings suggest IL-13-LCL-PLP might become a promising delivery platform for chemotherapeutic agents in melanoma.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"535-546"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0