Journal of Liposome Research最新文献_第4页

Development of optimized resveratrol/piperine-loaded phytosomal nanocomplex for isoproterenol-induced myocardial infarction treatment. 开发优化的白藜芦醇/哌啶载体植物纳米复合物，用于异丙肾上腺素诱导的心肌梗死治疗。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-07-12 DOI: 10.1080/08982104.2024.2378130

Thriveni Raunak Salian, Nadira Noushida, Sourav Mohanto, B H Jaswanth Gowda, Manodeep Chakraborty, Arfa Nasrine, Soumya Narayana, Mohammed Gulzar Ahmed

{"title":"Development of optimized resveratrol/piperine-loaded phytosomal nanocomplex for isoproterenol-induced myocardial infarction treatment.","authors":"Thriveni Raunak Salian, Nadira Noushida, Sourav Mohanto, B H Jaswanth Gowda, Manodeep Chakraborty, Arfa Nasrine, Soumya Narayana, Mohammed Gulzar Ahmed","doi":"10.1080/08982104.2024.2378130","DOIUrl":"10.1080/08982104.2024.2378130","url":null,"abstract":"Cardiovascular disease is a significant and ever-growing concern, causing high morbidity and mortality worldwide. Conventional therapy is often very precarious and requires long-term usage. Several phytochemicals, including Resveratrol (RSV) and Piperine (PIP), possess significant cardioprotection and may be restrained in clinical settings due to inadequate pharmacokinetic properties. Therefore, this study strives to develop an optimized RSV phytosomes (RSVP) and RSV phytosomes co-loaded with PIP (RPP) via solvent evaporation method using Box-Behnken design to enhance the pharmacokinetic properties in isoproterenol-induced myocardial infarction (MI). The optimized particle size (20.976 ± 0.39 and 176.53 ± 0.88 nm), zeta potential (-33.33 ± 1.5 and -48.7 ± 1.6 mV), drug content (84.57 ± 0.9 and 87.16 ± 0.6%), and %EE (70.56 ± 0.7 and 67.60 ± 0.57%) of the prepared RSVP and RPP, respectively demonstrated enhanced solubility and control release in diffusion media. The oral administration of optimized RSVP and RPP in myocardial infarction-induced rats exhibited significant (p < 0.001) improvement in heart rate, ECG, biomarker, anti-oxidant levels, and no inflammation than pure RSV. The pharmacokinetic assessment on healthy Wistar rats exhibited prolonged circulation (>24 h) of RSVP and RPP compared to free drug/s. The enhanced ability of RSVP and RPP to penetrate bio-membranes and enter the systemic circulation renders them a more promising strategy for mitigating MI.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"640-657"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141600272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, synthesis, and in vitro gene transfer efficacy of novel ionizable cholesterol derivatives. 新型可离子化胆固醇衍生物的设计、合成和体外基因转移功效。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-04-02 DOI: 10.1080/08982104.2024.2333755

Yajing Wang, Jiahui Jiang, Ziwei Ding, Tao Zhang, Yingying Shi, Xianfeng Huang, Xiaozhong Shen

{"title":"Design, synthesis, and in vitro gene transfer efficacy of novel ionizable cholesterol derivatives.","authors":"Yajing Wang, Jiahui Jiang, Ziwei Ding, Tao Zhang, Yingying Shi, Xianfeng Huang, Xiaozhong Shen","doi":"10.1080/08982104.2024.2333755","DOIUrl":"10.1080/08982104.2024.2333755","url":null,"abstract":"ABSTACTThe medicinal properties of genetic drugs are highly dependent on the design of delivery systems. Ionizable cationic lipids are considered core materials in delivery systems. However, there has not yet been a widespread consensus on the relationship between the wide diversity of lipid structure design and gene delivery efficiency. The aims of the research work were to synthesize ionizable cholesterol derivatives (iChol-lipids) and to evaluate their potential applications as gene delivery vector. A series of iChol-lipids with different head groups were synthesized with carbamate bond spacer. The chemical structures were characterized by 1H NMR, MS, melting range, and pKa. The interactions between iChol-lipids and MALAT1-siRNA were studied by molecular dynamics simulations and compared with market available DC-Chol, which revealed that hydrogen bonds, salt-bridge, and electrostatic interaction were probably involved. The self-assemble behaviors of these lipids were intensively investigated and evaluated by dynamic laser scattering in the presence of different helper lipids and PEGylated lipids. Their plasmid binding ability, transfection efficiency, hemolytic toxicity, and cytotoxicity were fully studied. IZ-Chol-LNPs was proved to be highly potential to effectively complex with DNA, and endosome escape mechanisms mediated by proton sponge effect was verified by pH-sensitive fluorescence probe BCFL.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"562-574"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remote loading in liposome: a review of current strategies and recent developments. 脂质体中的远程装载：当前策略和最新发展综述。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-02-11 DOI: 10.1080/08982104.2024.2315449

Navami Rajan Nambiar, Shreya Gaur, Gayathri Ramachandran, Ravi Shankar Pandey, Sabitha M, Lekshmi R Nath, Tathagata Dutta, M S Sudheesh

{"title":"Remote loading in liposome: a review of current strategies and recent developments.","authors":"Navami Rajan Nambiar, Shreya Gaur, Gayathri Ramachandran, Ravi Shankar Pandey, Sabitha M, Lekshmi R Nath, Tathagata Dutta, M S Sudheesh","doi":"10.1080/08982104.2024.2315449","DOIUrl":"10.1080/08982104.2024.2315449","url":null,"abstract":"Liposomes have gained prominence as nanocarriers in drug delivery, and the number of products in the market is increasing steadily, particularly in cancer therapeutics. Remote loading of drugs in liposomes is a significant step in the translation and commercialization of the first liposomal product. Low drug loading and drug leakage from liposomes is a translational hurdle that was effectively circumvented by the remote loading process. Remote loading or active loading could load nearly 100% of the drug, which was not possible with the passive loading procedure. A major drawback of conventional remote loading is that only a very small percentage of the drugs are amenable to this method. Therefore, methods for drug loading are still a problem for several drugs. The loading of multiple drugs in liposomes to improve the efficacy and safety of nanomedicine has gained prominence recently with the introduction of a marketed formulation (Vyxeos) that improves overall survival in acute myeloid leukemia. Different strategies for modifying the remote loading process to overcome the drawbacks of the conventional method are discussed here. The review aims to discuss the latest developments in remote loading technology and its implications in liposomal drug delivery.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"658-670"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcein release from DPPC liposomes by phospholipase A2 activity: Effect of cholesterol and amphipathic copolymers. 磷脂酶 A2 活性从 DPPC 脂质体中释放钙黄绿素：胆固醇和两性共聚物的影响。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-12-01 Epub Date: 2024-06-07 DOI: 10.1080/08982104.2024.2361610

Marco Soto-Arriaza, Eduardo Cena Ahumada, Sebastián Bonardd, Jaime Melendez

{"title":"Calcein release from DPPC liposomes by phospholipase A2 activity: Effect of cholesterol and amphipathic copolymers.","authors":"Marco Soto-Arriaza, Eduardo Cena Ahumada, Sebastián Bonardd, Jaime Melendez","doi":"10.1080/08982104.2024.2361610","DOIUrl":"10.1080/08982104.2024.2361610","url":null,"abstract":"In this study, we evaluated the impact of incorporating diblock and triblock amphiphilic copolymers, as well as cholesterol into DPPC liposomes on the release of a model molecule, calcein, mediated by exogenous phospholipase A2 activity. Our findings show that calcein release slows down in the presence of copolymers at low concentration, while at high concentration, the calcein release profile resembles that of the DPPC control. Additionally, calcein release mediated by exogenous PLA2 decreases as the amount of solubilized cholesterol increases, with a maximum between 18 mol% and 20 mol%. At concentrations higher than 24 mol%, no calcein release was observed. Studies conducted on HEK-293 and HeLa cells revealed that DPPC liposomes reduced viability by only 5% and 12%, respectively, after 3 hours of incubation, while DPPC liposome in presence of 33 mol% of Cholesterol reduced viability by approximately 11% and 23%, respectively, during the same incubation period. For formulations containing copolymers at low and high concentrations, cell viability decreased by approximately 20% and 40%, respectively, after 3 hours of incubation. Based on these preliminary results, we can conclude that the presence of amphiphilic copolymers at low concentration can be used in the design of new DPPC liposomes, and together with cholesterol, they can modulate liposome stabilization. The new formulations showed low cytotoxicity in HEK-293 cells, and it was observed that calcein release depended entirely on PLA2 activity and the presence of calcium ions.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"617-629"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-modified liposomal in-situ nasal gel enhances brain targeting of berberine hydrochloride for Alzheimer's therapy: optimization and in vivo studies. 表面修饰脂质体鼻腔原位凝胶增强了盐酸小檗碱治疗阿尔茨海默氏症的脑靶向性：优化和体内研究。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-11-25 DOI: 10.1080/08982104.2024.2431908

Sejal Bahndare, Dyandevi Mathure, Hemantkumar Ranpise, Malati Salunke, Rajendra Awasthi

{"title":"Surface-modified liposomal in-situ nasal gel enhances brain targeting of berberine hydrochloride for Alzheimer's therapy: optimization and in vivo studies.","authors":"Sejal Bahndare, Dyandevi Mathure, Hemantkumar Ranpise, Malati Salunke, Rajendra Awasthi","doi":"10.1080/08982104.2024.2431908","DOIUrl":"https://doi.org/10.1080/08982104.2024.2431908","url":null,"abstract":"This work aimed to formulate surface-modified berberine hydrochloride (BER)-loaded liposomes containing in-situ nasal gel for bran targeting. The liposomes were prepared by ethanol-injection method and optimized following a 32 full-factorial design. Size, morphology, zeta potential, ex-vivo permeation, and in-vitro release were estimated. The surface of optimized liposome was modified with ascorbic acid. The size of surface-modified liposomes was bigger (191.4 nm) than the unmodified liposomes (171 nm). Surface-modified liposomes were embedded in in-situ gel using poloxamer and Carbopol 934P. Liposomal in-situ gel showed higher permeation (71.94%) in contrast to the plain gel (46.64%). In-vivo pharmacokinetic examination of payload from liposomal in-situ gel displayed higher concentration in brain (Cmax of 93.50 ng/mL). The liposomal in-situ nasal gel had a higher drug targeting efficiency (138.43%) and a higher drug targeting potential (27.77%) confirming improved brain targeting. In male Wistar rats, the pharmacodynamic parameters (path length and escape latency) were evaluated with trimethyl tin-induced neurodegeneration. Animals treated with BER-loaded in-situ gel significantly decreased escape latency and path length in comparison to the control group. Histopathological assessment showed that the formulated gel was safe for intranasal administration. The developed formulation has the potential to effectively enhance the efficacy of BER in Alzheimer's disease management.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-18"},"PeriodicalIF":3.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of liposomal nanoparticles to load 4-farnesyloxycoumarin and investigating its anti-cancer and anti-metastatic effects. 合成脂质体纳米颗粒以载入 4-法尼酰氧基香豆素，并研究其抗癌和抗转移作用。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-11-17 DOI: 10.1080/08982104.2024.2428168

Shima Abbas Salman Al-Baidhani, Vahid Pouresmaeil, Masoud Homayouni Tabrizi

{"title":"Synthesis of liposomal nanoparticles to load 4-farnesyloxycoumarin and investigating its anti-cancer and anti-metastatic effects.","authors":"Shima Abbas Salman Al-Baidhani, Vahid Pouresmaeil, Masoud Homayouni Tabrizi","doi":"10.1080/08982104.2024.2428168","DOIUrl":"10.1080/08982104.2024.2428168","url":null,"abstract":"The aim of this study was to load 4-farnesyloxycoumarin (4-FLC) in nanoliposomes (4-FLC-LNPs) and evaluate its anti-cancer and anti-metastatic effects. 4-FLC-LNPs were synthesized using a combination of lecithin-cholesterol-polyethylene glycol. The physicochemical properties were evaluated using DLS, FTIR, and microscopy methods. The toxicity against breast cancer (MCF-7), prostate cancer (PS3), pancreatic cancer (PANC), gastric cancer (AGS), and normal cell lines (HUVEC) was evaluated using the MTT assay. Fluorescent staining and flow cytometry were used to assess the occurrence of apoptosis. Molecular analysis methods were used to study the apoptosis and metastasis effects of these nanoliposomes. The antioxidant power of 4-FLC-LNPs was measured using the ABTS and DPPH free radicals methods. 4-FLC-LNPs exhibit a spherical morphology, with an average size of 57.43 nm, a polydispersity index of 0.29, and a zeta potential of -31.4 mV. They demonstrate an encapsulation efficiency of 82.4% for 4-FLC. The IC50 value of 4-FLC-LNPs against the breast cancer cell line was reported as the most sensitive, at approximately 60 μg/mL. ABTS and DPPH results were reported at approximately 30 µg/mL. The inductive effects of nanoliposomes on the apoptosis process were confirmed by an increase in the number of apoptotic cells, as well as the arrest of cells in various phases of cell growth. The increased expression of BAX and decreased expression of Bcl-2, MMP-2, and MMP-9 confirmed the pro-apoptotic and anti-metastatic effects of 4-FLC-LNPs. These finding validate the therapeutic potential of 4-FLC-LNPs, which may be utilized in preclinical studies.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-10"},"PeriodicalIF":3.6,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics-based stable production of monodisperse giant unilamellar vesicles by oil-phase removal from double emulsion. 基于微流控技术，通过从双乳液中去除油相，稳定生产单分散巨型单酰胺囊泡。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-10-29 DOI: 10.1080/08982104.2024.2420337

Tomoki Yamada, Hiroaki Suzuki

引用次数: 0

Development and in vitro characterization of new carnosine-loaded liposomal formulations. 新型肉碱脂质体制剂的开发和体外表征。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-10-15 DOI: 10.1080/08982104.2024.2415664

Stefano Russo, Anna Privitera, Giuliana Greco, Lucia Di Pietro, Vincenzo Cardaci, Giuseppe Carota, Maria Grazia Sarpietro, Giuseppe Caruso

{"title":"Development and in vitro characterization of new carnosine-loaded liposomal formulations.","authors":"Stefano Russo, Anna Privitera, Giuliana Greco, Lucia Di Pietro, Vincenzo Cardaci, Giuseppe Carota, Maria Grazia Sarpietro, Giuseppe Caruso","doi":"10.1080/08982104.2024.2415664","DOIUrl":"https://doi.org/10.1080/08982104.2024.2415664","url":null,"abstract":"Carnosine is an endogenous dipeptide characterized by a multimodal mechanism of action. However, its clinical potential is limited by serum and cytosolic carnosinases, which significantly reduce its bioavailability. Based on that, different research groups have worked on the development of new strategies able not only to prevent its rapid metabolization but also to improve its distribution and specific targeting. In the present study, the development and in vitro characterization of new liposomal formulations loaded with carnosine are described. Nanoliposomes, produced through Thin-Layer Hydration followed by Extrusion method, were first investigated for their physicochemical stability. Photon correlation spectroscopy and electrophoretic light scattering, assessing the stability of the formulations, showed a strong homogeneity-oriented tendency for up to two months. Particle size, polydispersity index, and zeta potential were determined through dynamic light scattering and electrophoretic light scattering, demonstrating an almost neutral charge of the formulation and an effective encapsulation of carnosine. The morphology assessment performed via scanning electron microscopy showed good conformity and polydispersity. Differential scanning calorimetry measurements suggest the ability of carnosine to stabilize the large unilamellar vesicles. Lastly, the newly developed carnosine-loaded liposomal formulations also showed a good safety profile in human microglia.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-8"},"PeriodicalIF":3.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and characterization of niosomes for the delivery of a lipophilic model drug: comparative stability study with liposomes against phospholipase-A₂. 用于递送亲脂模型药物的niosomes的制备和表征：与针对磷脂酶-A2的脂质体的稳定性比较研究。

IF 3.6 4区医学

Journal of Liposome Research Pub Date : 2024-10-03 DOI: 10.1080/08982104.2024.2410748

Nazanin Kianinejad, Reza Razeghifard, Hossein H Omidian, Yadollah Omidi, Young M Kwon

{"title":"Preparation and characterization of niosomes for the delivery of a lipophilic model drug: comparative stability study with liposomes against phospholipase-A2.","authors":"Nazanin Kianinejad, Reza Razeghifard, Hossein H Omidian, Yadollah Omidi, Young M Kwon","doi":"10.1080/08982104.2024.2410748","DOIUrl":"https://doi.org/10.1080/08982104.2024.2410748","url":null,"abstract":"Vesicular nanocarriers like niosomes and liposomes are widely researched for controlled drug delivery systems, with niosomes emerging as promising alternatives due to their higher stability and ease of manufacturing. This study aimed to develop and characterize a niosomal formulation for the encapsulation and sustained release of temozolomide (TMZ), a model lipophilic drug, and to compare the stability of niosomes and liposomes, with a particular focus on the behavior of their lipid bilayers. Niosomes were prepared using the thin-film hydration method, composed of Span 60 (Sorbitan monostearate), cholesterol, and soy lecithin in varying molar ratios. The study investigated critical properties such as drug loading capacity, release kinetics, and resistance to enzymatic degradation. The optimized formulation was analyzed for drug entrapment efficiency and stability against phospholipase A2 (PLA2) degradation. The optimized niosomal formulation, with a 4:2:1 molar ratio of Span 60: cholesterol, achieved a high TMZ entrapment efficiency of 73.23 ± 1.02% and demonstrated sustained drug release over 24 hours. In comparison, liposomes released their TMZ payload within 4 hours upon exposure to PLA2, while the niosomes maintained their release profile, indicating superior stability. Spectroscopic and thermal analysis confirmed successful drug encapsulation with no component incompatibilities.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-12"},"PeriodicalIF":3.6,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of free vs. liposomal naringenin in white adipose tissue browning in C57BL/6j mice 游离与脂质体柚皮苷对 C57BL/6j 小鼠白色脂肪组织褐变的影响比较

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-09-19 DOI: 10.1080/08982104.2024.2405131

Kübra Uçar Baş, Aslıhan Ağaçdiken, Elif Didem Örs Demet, Dilem Tuğal Aslan, Tuba Reçber, Süleyman Can Öztürk, Tugba Gulsun, Mustafa Çelebier, Zeynep Göktaş

引用次数: 0