Journal of Liposome Research最新文献

pH-triggered liposomal delivery of alendronate enables acid-responsive drug release and improves osteoblast viability. ph触发的脂质体递送阿仑膦酸钠使酸反应性药物释放和改善成骨细胞的活力。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-10-13 DOI: 10.1080/08982104.2026.2744269

Kevin López Barrios, David Octavio Tovar Anaya, Rafael Arturo Zubillaga, Jesús Ángel Arenas Alatorre, Silvia Maldonado-Frías, Víctor Hugo Lara-Corona, Lauro Bucio, Marco Antonio Álvarez Pérez, Francisco Javier Marichi Rodriguez, Eduardo Villarreal-Ramírez

{"title":"pH-triggered liposomal delivery of alendronate enables acid-responsive drug release and improves osteoblast viability.","authors":"Kevin López Barrios, David Octavio Tovar Anaya, Rafael Arturo Zubillaga, Jesús Ángel Arenas Alatorre, Silvia Maldonado-Frías, Víctor Hugo Lara-Corona, Lauro Bucio, Marco Antonio Álvarez Pérez, Francisco Javier Marichi Rodriguez, Eduardo Villarreal-Ramírez","doi":"10.1080/08982104.2026.2744269","DOIUrl":"https://doi.org/10.1080/08982104.2026.2744269","url":null,"abstract":"Bisphosphonates, such as sodium alendronate (ALN), are primary treatments for osteoporosis and osteosarcoma; however, their long-term use is limited by causing severe side effects. A pH-sensitive liposomal formulation was developed to enhance ALN delivery, enabling controlled drug release specifically in acidic biological microenvironments and niches. Release efficiency of pH-sensitive liposomes was evaluated and compared with that of standard and PEGylated liposomes. Molecular dynamics simulations demonstrated the stability and structural behavior of liposome formulations under physiological conditions, both with and without ALN. Membrane properties were consistent with experimental data. Liposomes were synthesized by the lipid bilayer rehydration method and characterized in vitro for vesicle size, zeta potential, lipid quantification, encapsulation efficiency, and release kinetics. Morphology was assessed by AFM and SEM. pH-sensitive liposomes released only 12% of ALN over 8 h under neutral pH conditions, while acidic conditions triggered a complete (100%) release. In hFOB 1.19 osteoblasts, pH-sensitive liposomes increased cell viability by 60% compared to free ALN in solution. To the best of current knowledge, no previously reported liposomal formulation has demonstrated complete (100%) ALN release under acidic conditions. The resulting pH-sensitive liposomes, with a mean diameter of 126 nm, exhibit physicochemical characteristics compatible with intravenous administration. The computational and experimental methodology presented here may be useful for the design and optimization of formulations for ALN and other bisphosphonates. Further in vitro and in vivo validations are needed to assess their performance and potential applications.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-18"},"PeriodicalIF":4.7,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Critical factors influencing the precise ultrasound-mediated production of lipid nanoparticles. 影响超声介导的脂质纳米颗粒精确生产的关键因素。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-10-11 DOI: 10.1080/08982104.2026.2742362

Christopher Sánchez-Vega, Ramon Barnadas-Rodríguez

{"title":"Critical factors influencing the precise ultrasound-mediated production of lipid nanoparticles.","authors":"Christopher Sánchez-Vega, Ramon Barnadas-Rodríguez","doi":"10.1080/08982104.2026.2742362","DOIUrl":"https://doi.org/10.1080/08982104.2026.2742362","url":null,"abstract":"Due to their characteristics, sonicators are widely used in the pharmaceutical and food industries for the preparation of stable suspensions and oil-in-water emulsions. Ultrasound-assisted processing enables the production of lipid nanoparticles, which are of considerable interest as drug delivery systems. However, despite their widespread use, the influence of the numerous parameters governing ultrasound-mediated lipid nanoparticle formation remains insufficiently characterized. In this study, a systematic and comprehensive investigation was conducted to evaluate the effects of key experimental variables on the size distribution of lipid nanoparticles produced via microprobe sonication. Liposomes composed of a natural phospholipid were employed as model systems. Instrument-related parameters, including nominal and effective power, treatment time, and probe positioning, were examined alongside sample characteristics such as temperature, lipid concentration, dispersion volume, and lipid composition. In parallel, the extent of phospholipid degradation induced by sonication was assessed in relation to the formation of reactive species during cavitation. The results provide quantitative relationships describing particle size evolution as a function of the most relevant factors and suggest that sonication exhibits a behavior analogous to that of a continuous stirred-tank reactor and high-pressure homogenizers. They also indicate that, under the studied conditions, phospholipids remain chemically unaltered. Overall, the aim of this work is to develop a coherent predictive framework for ultrasound-mediated lipid nanoparticle synthesis and to offer practical guidance for process optimization. The proposed framework is expected to be broadly applicable to related colloidal systems, including emulsions and suspensions, in pharmaceutical and food applications across both academic and industrial settings.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-11"},"PeriodicalIF":4.7,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering and optimization of microfluidic platforms for phospholipid-based ocular drug delivery. 基于磷脂的眼部给药微流控平台的工程与优化。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-10-06 DOI: 10.1080/08982104.2026.2740639

Shakila Dikowita Kankanamlage, Thilini Thrimawithana, Francisco J Tovar-Lopez

{"title":"Engineering and optimization of microfluidic platforms for phospholipid-based ocular drug delivery.","authors":"Shakila Dikowita Kankanamlage, Thilini Thrimawithana, Francisco J Tovar-Lopez","doi":"10.1080/08982104.2026.2740639","DOIUrl":"https://doi.org/10.1080/08982104.2026.2740639","url":null,"abstract":"Ocular drug delivery remains a major challenge due to anatomical, physiological, and pathological barriers that limit drug penetration and retention. Liposomes offer a promising strategy to address these constraints; however, their effectiveness depends on physicochemical properties such as size, surface charge, and interfacial functionality, which govern transport across ocular tissues. Microfluidic technology provides a powerful platform to achieve precise control over these properties through tunable flow conditions and controlled self-assembly, enabling the reproducible fabrication of liposomes with tailored characteristics. In this context, this review establishes a barrier-informed framework that links ocular transport limitations to liposome design requirements and microfluidic processing strategies. In this review, we systematically examine microfluidic mixer geometries, including hydrodynamic flow, Dean-flow-based designs, and chaotic advection mixers, and discuss their impact on liposome formation. Methods for evaluating mixing performance are reviewed alongside the advantages and limitations of different chip materials and fabrication approaches. In addition, strategies for tuning liposome properties, including size, polydispersity, lamellarity, and stability, are critically analyzed in relation to ocular delivery requirements. Finally, emerging directions such as integrated single-step platforms, hybrid and 3D-printed systems, machine learning-assisted process control, solvent-free fabrication, and non-spherical liposomes are discussed. Collectively, this review provides a conceptual and engineering framework for the rational design of microfluidic platforms for liposome-based ocular drug delivery, bridging transport physics with translational applications.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-30"},"PeriodicalIF":4.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary delivery of ivacaftor via proliposomal dry powder inhalers for cystic fibrosis therapy. 经原脂质体干粉吸入器肺输送ivvacaftor治疗囊性纤维化。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-10-06 DOI: 10.1080/08982104.2026.2738469

Akanksha Kasture, Himanshu Paliwal, Nilesh Chaudhari, Bhupendra G Prajapati

{"title":"Pulmonary delivery of ivacaftor via proliposomal dry powder inhalers for cystic fibrosis therapy.","authors":"Akanksha Kasture, Himanshu Paliwal, Nilesh Chaudhari, Bhupendra G Prajapati","doi":"10.1080/08982104.2026.2738469","DOIUrl":"https://doi.org/10.1080/08982104.2026.2738469","url":null,"abstract":"Ivacaftor, a CFTR potentiator, is clinically effective in treating cystic fibrosis (CF), but it has poor lung targeting, variable oral bioavailability, and poor aqueous solubility. The objective of the study was to develop and optimize an inhalable proliposomal dry powder inhaler (DPI) of ivacaftor for pulmonary delivery. The optimized ivacaftor-loaded proliposomes were optimized using a face-centered central composite design (α = 1) by varying soy phosphatidylcholine (SPC) concentration and drug: cholesterol ratio, with particle size, zeta potential, and entrapment efficiency. The optimized formulation was spray dried with mannitol and leucine to obtain inhalable microparticles. The optimized proliposomal formulation had nanosized vesicles (∼198 nm), high entrapment efficiency (∼73%), and good colloidal stability. The solid-state characterization confirmed amorphization and molecular dispersion of ivacaftor without chemical incompatibility. The spray-dried formulation exhibited high emitted dose (96.45%), fine particle fraction (52.38%), and MMAD (1.66 µm), and sustained drug release, good cytocompatibility, and satisfactory accelerated stability supported its suitability. Conclusively, proliposomal inhalation delivery is a promising approach to enhance pulmonary targeting of ivacaftor in cystic fibrosis.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-12"},"PeriodicalIF":4.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposomal nanoparticle mediated gene modulation for the treatment of melanoma in murine models. 脂质体纳米颗粒介导的基因调节治疗小鼠黑色素瘤模型。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-10-01 DOI: 10.1080/08982104.2026.2738804

Poonam Yadav, Patience J Kelly, Santosh K Mandal, Malea Williams, Bill Tang, Amanda McWatters, Rahul A Sheth

{"title":"Liposomal nanoparticle mediated gene modulation for the treatment of melanoma in murine models.","authors":"Poonam Yadav, Patience J Kelly, Santosh K Mandal, Malea Williams, Bill Tang, Amanda McWatters, Rahul A Sheth","doi":"10.1080/08982104.2026.2738804","DOIUrl":"https://doi.org/10.1080/08982104.2026.2738804","url":null,"abstract":"Melanoma is a treatment-resistant and highly aggressive form of skin cancer. The aberrant activation of the Wnt/β-catenin pathway is a key driver of proliferation, tumor progression, and metastasis in many cancers, including melanoma. The active involvement in tumor growth and immune evasion makes the Wnt/β lipoplexes-catenin pathway a critical therapeutic target. Recent advancements in gene delivery systems, such as liposomal nanoparticles (LNPs), have opened new possibilities for targeted cancer therapies by efficiently delivering genetic material, including microRNA (miRNA), to cancer cells with minimal off-target effects. Among potential gene-based therapies, miR-124 has emerged as a promising candidate for targeting the Wnt/β-catenin pathway. However, a major challenge in miRNA-based therapeutics is the efficient delivery of miRNA to tumor cells. By using cationic liposomes to form lipoplexes with miR-124 plasmids, we aim to evaluate the efficiency of lipoplex-mediated delivery and transfection in both in vitro and in vivo melanoma models and observe their effects on WNT/β-catenin signaling and tumor growth. The application of lipoplexes in gene-based therapies represents a promising approach to improve treatment outcomes for melanoma patients.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-13"},"PeriodicalIF":4.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetic comparisons between liposome formulation and non-liposome formulation of omega-3 fatty acids: a randomized clinical study. omega-3脂肪酸脂质体制剂和非脂质体制剂的药代动力学比较：一项随机临床研究。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-10-01 DOI: 10.1080/08982104.2026.2721669

Yun Seob Jung, Ae Gyeoing Im, Sung Dae Kwon, Tae Gi Kim, Dong Woo Chae

引用次数: 0

α-Tocopherol-loaded freeze-dried liposomes and proliposomes: powder properties and thermal performance. α-生育酚负载的冷冻干燥脂质体和原脂质体：粉末性质和热性能。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-09-30 DOI: 10.1080/08982104.2026.2740627

Jiyoon Lee, Hye Won Kim, Hyeon Gyu Lee, In Young Bae

{"title":"α-Tocopherol-loaded freeze-dried liposomes and proliposomes: powder properties and thermal performance.","authors":"Jiyoon Lee, Hye Won Kim, Hyeon Gyu Lee, In Young Bae","doi":"10.1080/08982104.2026.2740627","DOIUrl":"https://doi.org/10.1080/08982104.2026.2740627","url":null,"abstract":"Dry lipid-based formulations can enhance the handling and preservation of lipophilic bioactives, though their efficacy depends heavily on formulation composition and processing conditions. This study compared α-tocopherol-loaded freeze-dried liposomes (FD_LPs) and proliposomes (PLPs) formulated with either mannitol (PLPs_MAN) or trehalose (PLPs_TRE) as carriers. All three formulations were evaluated for reconstitution characteristics, powder properties, and thermal performance after rehydration. Additionally, a four-week storage stability test was conducted at 4, 25, and 40 °C, restricted to FD_LPs and PLPs_MAN. Upon reconstitution, all formulations exhibited mean vesicle diameters ranging from 561.5 to 669.3 nm and polydispersity index (PDI) values of approximately 0.57-0.60, indicating relatively broad particle size distributions. Initial entrapment efficiencies (EE) were high, exceeding 93% for all samples. Among the formulations, PLPs_MAN exhibited the lowest moisture content. Both PLP formulations demonstrated lower hygroscopicity and superior flow indices compared to FD_LPs. Under thermal stress (90 °C, 12 h), PLPs_TRE showed the lowest TBARS response, whereas PLPs_MAN maintained the highest DPPH radical-scavenging activity. After four weeks at 40 °C, mean EE values were lower than their initial values for both tested formulations, reaching 73.7% for FD_LPs and 79.0% for PLPs_MAN. Overall, both proliposome formulations exhibited more favorable powder flow indices and lower hygroscopicity than FD_LPs, whereas their thermal responses depended on the endpoint examined. The four-week storage findings apply only to FD_LPs and PLPs_MAN and do not establish the storage performance of PLPs_TRE. Differences in carrier loading and processing limit attribution of the observed effects to carrier identity alone.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-8"},"PeriodicalIF":4.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipodisks for dual delivery of pirarubicin and melittin: physicochemical properties and in vitro evaluation. 双重递送吡柔比星和蜂毒素的脂盘：理化性质和体外评价。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-09-28 DOI: 10.1080/08982104.2026.2738451

Luís Silva, Wahida Rahaman, Sajid Ali, Lars Gedda, Víctor Agmo Hernández, Katarina Edwards

{"title":"Lipodisks for dual delivery of pirarubicin and melittin: physicochemical properties and in vitro evaluation.","authors":"Luís Silva, Wahida Rahaman, Sajid Ali, Lars Gedda, Víctor Agmo Hernández, Katarina Edwards","doi":"10.1080/08982104.2026.2738451","DOIUrl":"https://doi.org/10.1080/08982104.2026.2738451","url":null,"abstract":"Nanocarrier-based combination therapy can enhance anticancer efficacy by reducing systemic toxicity, overcoming drug resistance, and enabling synergistic interactions, but requires nanocarriers capable of efficiently loading and retaining structurally diverse therapeutic agents. While lipid-based nanoparticles such as liposomes are widely employed due to their intrinsic biocompatibility, they are often unsuitable for membranolytic compounds like the peptide melittin. Polyethylene glycol (PEG)-stabilized lipodisks, discoidal lipid assemblies with well-defined high curvature rim domains, have recently been shown to accommodate therapeutically relevant quantities of melittin together with doxorubicin. Here, we evaluate PEG-stabilized lipodisks as co-delivery vehicles for melittin and tetrahydropyranyl adriamycin (THP, pirarubicin), a more potent and less hydrophilic anthracycline. We examine how lipid composition affects THP loading and retention, identify factors governing melittin binding, and evaluate cytotoxicity of lipodisk delivering each drug individually or in combination. Although the lipodisks exhibit somewhat lower loading capacity for THP compared to doxorubicin, the retention of the former is markedly superior. Importantly, the potential for synergistic effects is clearly greater when THP and melittin are co-delivered via dual-loaded lipodisks compared to administered as free drug mixtures (average CI: 0.83 ± 0.17 vs. 1.11 ± 0.21). These findings highlight PEG-stabilized lipodisks as a promising platform for anthracycline-melittin combination therapy.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-13"},"PeriodicalIF":4.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical assay interference and temporal redox retention in liposome-associated Trolox under mixed-metal stress. 混合金属应力下脂质体相关Trolox的光学分析干扰和时间氧化还原保留。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-09-19 DOI: 10.1080/08982104.2026.2735841

Hasan Saygin, Montaser N A Ramadan, Asli Baysal, Sinem Karniyarik, Münevver Müge Çağal, Kubra Bezir

{"title":"Optical assay interference and temporal redox retention in liposome-associated Trolox under mixed-metal stress.","authors":"Hasan Saygin, Montaser N A Ramadan, Asli Baysal, Sinem Karniyarik, Münevver Müge Çağal, Kubra Bezir","doi":"10.1080/08982104.2026.2735841","DOIUrl":"https://doi.org/10.1080/08982104.2026.2735841","url":null,"abstract":"Absorbance-based antioxidant assays are widely used to characterize liposomal formulations, yet lipid vesicles scatter light and can contribute signal unrelated to redox activity. Using Trolox as a model antioxidant, we examined how liposome association alters the measured redox response over time, and how carrier-dependent optical interference affects its interpretation under a mixed-metal stress challenge. Free Trolox, empty liposomes and liposome-associated Trolox were followed over 0-48 h without and with a commercial 23-element metal mixture (10-1000 µg/L total), using dithiothreitol (DTT), Cupric Reducing Antioxidant Capacity (CUPRAC), Trolox Equivalent Antioxidant Capacity (TEAC) and Folin-Ciocalteu assays with parallel turbidity, plus a composition-matched physical mixture in an exploratory Escherichia coli panel. Empty liposomes, containing no antioxidant, gave TEAC and Folin signals exceeding free Trolox and shared the same principal-component region as liposome-associated Trolox, so the combined axis reflects a redox-optical response rather than antioxidant capacity. DTT provided the clearest separation between the free-Trolox response and the empty-carrier background (free/empty ≈ 3.3), although it remained associated with turbidity. Without metal, the liposome-associated form declined more slowly on DTT (retention 0.89 versus 0.62). The metal mixture reduced the DTT response to near-background levels at every tested non-zero dose and exerted a dominant negative effect across the multichannel model. At 1000 µg/L, liposome-associated Trolox and the physical mixture were indistinguishable, showing no association-specific benefit. Empty-carrier, turbidity and composition-matched controls are essential to avoid overestimating liposomal antioxidant activity.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-13"},"PeriodicalIF":4.7,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149008440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction-responsive cationic bolaamphiphile vesicles as a versatile platform for photodynamic and chemotherapeutic delivery. 还原反应性阳离子亲水分子囊泡作为光动力和化疗递送的多功能平台。

IF 4.7 4区医学

Journal of Liposome Research Pub Date : 2026-09-02 DOI: 10.1080/08982104.2026.2726858

Begoña Bedrina, César A Angulo-Pachón, Francisco Galindo, Juan F Miravet

{"title":"Reduction-responsive cationic bolaamphiphile vesicles as a versatile platform for photodynamic and chemotherapeutic delivery.","authors":"Begoña Bedrina, César A Angulo-Pachón, Francisco Galindo, Juan F Miravet","doi":"10.1080/08982104.2026.2726858","DOIUrl":"https://doi.org/10.1080/08982104.2026.2726858","url":null,"abstract":"A reduction-sensitive cationic bolaamphiphile bearing N-methylpyridinium terminal groups and a central disulfide bridge was synthesized and shown to self-assemble in water into stable vesicles with diameters of 200-250 nm. The vesicles undergo redox-triggered disassembly in the presence of tris(2-carboxyethyl)phosphine (TCEP), and analogous behavior was also observed using glutathione (GSH), supporting their responsiveness to reductive environments. Their potential as nanocarriers was evaluated using the photosensitizer Rose Bengal (RB) and the chemotherapeutic agents paclitaxel (PTX) and camptothecin (CPT) in A549, HT29, and MCF7 cancer cell lines. The vesicles significantly enhanced the cellular uptake of RB, as demonstrated by flow cytometry and confocal microscopy. Colocalization analysis with MitoTracker Green further supported preferential mitochondrial localization of RB fluorescence. This was accompanied by a pronounced increase in photodynamic activity upon light irradiation in A549 and MCF7 cells, where apoptosis was identified as the dominant cell-death pathway. In addition, vesicle-based formulations of PTX and CPT improved their cytotoxicity across all tested cell lines, with a particularly strong effect observed in HT29 cells under the experimental conditions employed. Overall, these pyridinium-based bolaamphiphilic vesicles represent a versatile redox-responsive delivery platform capable of enhancing intracellular delivery and therapeutic performance of both photosensitizers and poorly water-soluble anticancer drugs.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-12"},"PeriodicalIF":4.7,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0