Journal of Liposome Research最新文献

{"title":"<i>In vitro</i> cytotoxicity of liposomal stearyl triphenyl phosphonium is influenced by mitochondrial bioenergetics.","authors":"Shrey Shah, Rajitha Gadde, Maitri Shukla, Hiral Tanna, Purvajeet P Jadeja, Himani Bhagwat, Ruchita Joshi, Snigdha Suluru, Gerard G M D'Souza","doi":"10.1080/08982104.2025.2567860","DOIUrl":"https://doi.org/10.1080/08982104.2025.2567860","url":null,"abstract":"<p><p>Stearyl triphenyl phosphonium (STPP) has previously been utilized to prepare mitochondriotropic liposomes for improved drug delivery to mitochondria. Additionally, liposomal STPP has shown higher toxicity toward drug-resistant tumor cells compared to non-drug-resistant tumor cells. The differential toxicity is hypothesized to be due to the differences in mitochondrial bioenergetics between drug-resistant and non-drug-resistant cells. This study aimed to further test the hypothesis by using drug-resistant and non-drug-resistant cell lines from ovarian, lung, uterine and breast carcinomas. Mitochondrial function was assessed by JC-1 dye accumulation and by measurement of oxygen consumption rate. Liposomal STPP cytotoxicity was then assessed using a metabolic assay. The drug-resistant ovarian, lung and uterine carcinoma cell lines showed higher mitochondrial activity compared to their non-drug-resistant counterparts while there was no difference in mitochondrial activity between drug-resistant and non-drug-resistant breast carcinoma cell lines. Consistent with the hypothesis of the study, liposomal STPP showed greater toxicity to the drug-resistant ovarian and uterine carcinoma cell lines compared to the non-drug-resistant cell lines and no difference in toxicity between drug-resistant and non-drug-resistant breast carcinoma cell lines. While liposomal STPP was more toxic toward the non-drug-resistant cell line in the case of lung carcinoma, the effect is likely due to the use of altered growth conditions for the non-drug-resistant cell line that were needed to allow testing by the assays used in the study. Collectively, the results suggest the potential for exploiting mitochondrial bioenergetic differences to target drug-resistant and non-drug-resistant tumor cell populations in a variety of tumor types.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-6"},"PeriodicalIF":4.3,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposome stability: multifactorial regulation and optimization strategies in in vivo delivery.","authors":"Xiao-Si Lu, Li-Qing Yang, Lan Ma, Jiang-Feng Wu, Hong-Bing Zhang, Ying Wang","doi":"10.1080/08982104.2025.2561825","DOIUrl":"https://doi.org/10.1080/08982104.2025.2561825","url":null,"abstract":"<p><p>As an essential carrier for drug delivery systems, the stability of liposomes directly influences the efficiency and safety of drug delivery. In the complex and challenging process of in vivo delivery, the stability of liposomes is affected by both their inherent properties and external environmental factors. This paper first introduces the in vivo journey of liposomes, encompassing blood circulation, tissue distribution, metabolism, and excretion. It then summarizes the biological factors affecting liposome stability, including immune recognition, protein-lipid interactions, enzyme-catalyzed degradation, and physiological changes. Additionally, the paper explores effective strategies to enhance liposome stability, such as optimizing lipid composition and surface modification. Finally, it discusses future research directions and existing challenges, focusing on AI-assisted liposome development, and the development of biocompatible materials. The study emphasizes the critical factors affecting liposome stability, uncovers current limitations, and highlights future potential in this field, providing a theoretical foundation and practical guidance for the design and application of liposomes.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-12"},"PeriodicalIF":4.3,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation, characterization, and <i>in vitro</i> evaluation of lactoferrin conjugated liposome loaded with magnesium sulfate for improved CNS penetrability.","authors":"Aparna Ann Mathew, Rajitha Panonnummal, Anand Kumar A, Sabitha M","doi":"10.1080/08982104.2025.2560888","DOIUrl":"https://doi.org/10.1080/08982104.2025.2560888","url":null,"abstract":"<p><p>Magnesium deficiency has been reported in association with various disease conditions, particularly in neurological diseases. The limited clinical use of magnesium sulfate is due to its restricted entry into the Central Nervous system (CNS) through blood brain barrier (BBB) which may consequently result in the peripheral accumulation followed by its toxic effects. The current study is focused on the development of nano formulation of lactoferrin conjugated liposome loaded with magnesium sulfate (LMGPLS) with an aim to improve its CNS permeability. LMGPLS was prepared by thin-film hydration technique followed by the lactoferrin conjugation. The average particle size of the LMGPLS was found to be 273.23 ± 6.7 nm. <i>In vitro</i> drug release studies showed that the drug release started after 15 min and continued up to 5 h from LMGPLS. The parallel artificial membrane permeability assay proved the improved lipid layer permeation of prepared formulation when compared with that of the drug solution. The prepared LMGPLS were found to be haemo-compatible and cyto-compatible when tested with blood as well as with multiple cell lines. The pharmacological effects of the formulations were evaluated by multiple <i>in vitro</i> experiments. The results from <i>in vitro</i> experiments showed that the developed formulation is capable of improving the cell viability in glutamate and H₂O₂ treated cell lines indicating its antioxidant and anti-excitotoxicity effects. Additionally, the developed liposome showed significant effect on digoxin induced Na⁺/K⁺ ATPase inhibition. Good lipid layer penetrability with haemo-compatibility and cytocompatibility warrant that the formulation will be a suitable candidate to increase the CNS delivery of Magnesium.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-13"},"PeriodicalIF":4.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-action liposome-peptide formulation synergistically counteracts a gain-of-function p53 mutant.","authors":"Sneha Ghosh Chaudhary, Swati Bhowmick, Samriddhi Bhattacharya, Siddhartha Roy, Nahid Ali","doi":"10.1080/08982104.2025.2555179","DOIUrl":"10.1080/08982104.2025.2555179","url":null,"abstract":"<p><p>Inactivation of p53 tumor suppressor functions, often through missense mutations, is essential for carcinogenesis. A sub-class of such p53 missense mutations gains new functions, including drug resistance and enhanced proliferation, in addition to its loss of function. Among the most frequent gain-of-function p53 mutants, R273H occurs in tumors of many tissue origins and imparts aggressive character and resistance to drugs to the tumor. Tumors bearing p53R273H are generally resistant to all available therapies, and need for novel interventions are urgently needed. Interaction of p53R273H with Positive Coactivator 4 (PC4), an abundant chromatin-associated protein, is essential for acquiring the gain-of-function properties. Previously, we developed a chemically modified peptide, NLS-p53(380-386), targeting PC4 that abrogated the interaction of p53R273H with PC4 and reversed many of its gain-of-function properties. We earlier demonstrated that cationic phosphatidylcholine-stearylamine (PC-SA) liposomes possess inherent anti-tumor properties. To improve efficacy, pharmacokinetics, and delivery, we entrapped the PC4-targeted peptide into PC-SA liposome. We synthesized the NLS-p53(380-386) peptide and entrapped in PC-SA liposome. We used MTT assay, confocal microscopy, flow cytometry, qRT-PCR, and western blotting to investigate the biological effects of the p53-entrapped PC-SA. Pretreatment with the PC-SA liposome entrapped peptide enhanced the chemosensitivity of widely used anticancer drug doxorubicin in cell lines bearing p53R273H mutation. The doxorubicin-induced cell-killing effect was much more enhanced when pretreated with the liposome-entrapped peptide than when pretreated with either the free peptide or the liposome alone. The liposome-encapsulated peptide is a promising formulation for developing therapies targeting tumors bearing the p53R273H.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-13"},"PeriodicalIF":4.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/08982104.2025.2499787","DOIUrl":"10.1080/08982104.2025.2499787","url":null,"abstract":"","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"i"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of PEG-modified isoquercitrin liposomes and anti-chronic kidney disease research.","authors":"Qinyang Hua, Qilong Wang, Xue Wang, Xia Jiang, Mingjie Gong, Jiaying Li, Tingyuan Li, Xiaowen Wang, Xia Cao, Jiangnan Yu, Elmurat Toreniyazov, Bin Zong, Ximing Xu, Feng Shi, Michael Adu-Frimpong","doi":"10.1080/08982104.2025.2480782","DOIUrl":"10.1080/08982104.2025.2480782","url":null,"abstract":"<p><p>The clinical application of Isoquercitrin (IQ) is limited by its low water solubility and short retention time in the body, despite its diverse pharmacological effects. To address these issues, we prepared polyethylene glycol (PEG)-modified IQ liposomes (IQ-L) using the thin film dispersion method and optimized the formulation through a combination of One Factor at a Time (OFAT) method and response surface experiments. Characterization of the IQ-L that was prepared using the optimal formulation revealed a particle size of 185.48 nm, a polydispersity index of 0.252, a zeta potential of -33.88 mV, and an impressive encapsulation efficiency of 97.84%. In vitro release studies showed a significantly higher cumulative release rate for IQ-L compared to free IQ. Pharmacokinetic evaluations in rats demonstrated a 4.54-fold increase in the area under the concentration-time curve, a 1.63-fold prolongation of the half-life, and a 2.07-fold increase in peak concentration for IQ-L compared to unmodified IQ. Moreover, assessments of renal function in a mouse model indicated promising therapeutic effects. In summary, the PEG-modified liposome system greatly improved the solubility and in vivo retention time of IQ, thus making it a potential clinical agent for the treatment of chronic kidney disease (CKD).</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"252-266"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomes incorporating cyclodextrins as a promising drug delivery system to augment the bioavailability of poorly soluble drugs.","authors":"Manar Adel Abdelbari, Shaimaa Mosallam","doi":"10.1080/08982104.2025.2473335","DOIUrl":"10.1080/08982104.2025.2473335","url":null,"abstract":"<p><p>Poorly water-soluble drugs are common and challenging in pharmaceutical industry. The poor solubility can reduce the drugs' therapeutic efficiency and bioavailability. Improving the solubility and bioavailability of poorly water-soluble drugs is a challenge and a main issue in the development and application of these drugs in pharmaceutical industry. Liposomes is phospholipid-based vesicular drug delivery system which act as drug carriers for both lipophilic or hydrophilic drugs and have the ability to solubilize the carried drugs. An innovative approach is the incorporation of cyclodextrins (CDs) into the liposomes encapsulating the drug molecules for improving safety and effectiveness of drug molecules by the use of water-soluble CDs properties, which allow the formation of CDs liposomes complexes with lipophilic molecules. CDs liposomes complex increases targeting effect, regulates drug release, improves drug properties and drug encapsulation efficiency. The aim of this review is to summarize the advantages and applications of liposomes incorporating CDs to enhance bioavailability of poorly soluble drugs.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"312-320"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of PEGylation for cationic triacyl lipid-based siRNA lipoplexes prepared using the modified ethanol injection method for tumor therapy.","authors":"Yoshiyuki Hattori, Mizuki Shinkawa, Aya Kurihara, Ryohei Shimizu","doi":"10.1080/08982104.2025.2498956","DOIUrl":"10.1080/08982104.2025.2498956","url":null,"abstract":"<p><p>We previously developed a modified ethanol injection (MEI) method to construct small interfering RNA (siRNA) lipoplexes by mixing a lipid-ethanol solution with an siRNA-containing phosphate-buffered saline solution. Here, we constructed siRNA lipoplexes with 11-((1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl)propan-2-yl)amino)-<i>N</i>,<i>N</i>,<i>N</i>-trimethyl-11-oxoundecan-1-aminium bromide (TC-1-12), 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphoethanolamine, and poly(ethylene glycol) (PEG)-lipid using our MEI method. The siRNA lipoplexes were PEGylated with 1, 3, 5, and 10 mol% PEG cholesteryl ether (PEG-Chol), 1,2-dimyristoyl-<i>rac</i>-glycero-3-methoxypolyethylene glycol (mPEG-DMG), or 1,2-distearoyl-<i>sn</i>-glycero-3-phosphoethanolamine-<i>N</i>-(methoxy[polyethylene glycol]) (mPEG-DSPE). PEGylation of siRNA lipoplexes with PEG-Chol did not attenuate the inhibitory effects of Luc and polo-like kinase 1 (PLK1) siRNA lipoplexes on the luciferase (Luc) activity and proliferation of human cervical carcinoma HeLa-Luc, human ovarian cancer SK-OV-3-Luc, and human breast cancer MCF-7-Luc cells stably expressing Luc. In contrast, PEGylated lipoplexes with 10 mol% mPEG-DMG inhibited Luc activity by Luc siRNA but considerably attenuated the PLK1 siRNA-mediated cytotoxic effects. For PEGylated siRNA lipoplexes with mPEG-DSPE, inhibitory effect of Luc siRNA on Luc activity decreased with increasing amounts of PEG modification, and PLK1 siRNA-mediated cytotoxic effects disappeared at more than 3 mol% PEGylation. Erythrocyte aggregation and hemolysis induction by the siRNA lipoplexes were effectively inhibited by 10 mol% PEGylation, irrespective of the PEG-lipid. Compared to those with 1 mol% PEG-Chol, PEGylated siRNA lipoplexes with 10 mol% PEG-Chol potently reduced siRNA accumulation in mouse lungs post-intravenous administration. Overall, TC-1-12-based siRNA lipoplexes with 10 mol% PEG-Chol exerted PLK1 siRNA-mediated cytotoxic effects, without inducing hemolysis and erythrocyte aggregation.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"300-311"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEGylated liposomes via ATRP for brain drug delivery.","authors":"Lulu Chen, Zhenfang Zhang, Maria R Simonsen, Trevor Owens, Reza M H Khorooshi, Changzhu Wu","doi":"10.1080/08982104.2025.2485428","DOIUrl":"10.1080/08982104.2025.2485428","url":null,"abstract":"<p><p>PEGylated liposomes play a critical role in drug delivery systems because they can evade immune recognition. However, conventional methods for synthesizing PEGylated liposomes often involve the direct incorporation of PEG-functionalized lipids, resulting in insufficient and inconsistent PEG distribution on the liposome surface, which compromises their stability and performance. In this study, we present a proof-of-concept synthesis approach that utilizes lipid-based initiators to form liposomes, followed by controllable grafting of PEG chains through atom transfer radical polymerization (ATRP). This method ensures controlled and uniform PEG coverage, resulting in improved functionality. Compared to conventional liposomes, the polymer-grafted liposomes synthesized via ATRP demonstrated superior cellular uptake <i>in vitro</i>, enhanced penetration of the blood-brain barrier (BBB), and improved stability <i>in vivo</i>, particularly for protein-encapsulated formulations such as green fluorescent protein (GFP). Live/dead assays confirmed the biocompatibility of the ATRP-synthesized PEGylated liposomes. Therefore, our strategy significantly enhances the efficiency of PEGylated liposomes for targeted brain drug delivery, providing a promising platform for the treatment of neurological disorders.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"283-289"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional liposomal gel in regenerative medicine.","authors":"Meghna Mohandas, Jayakumar Rangasamy","doi":"10.1080/08982104.2025.2480786","DOIUrl":"10.1080/08982104.2025.2480786","url":null,"abstract":"<p><p>The synergistic approach of liposome integrated with gel matrix could reshape the current frameworks of drug delivery technology. The liposome-based approaches are limited by inadequate stability and rapid leakage of drug molecules. Undesired and immediate drug release from gel increases the local concentration of drug and causes toxicity. So, the stabilization of liposomes within a gel matrix can be an effective option to provide an ingenious solution to the conventional limitation on short half-life, instability, toxicity, uncontrolled drug release and poor retention of drug molecules on the target site. The capability to incorporate antibacterial as well as anti-oxidant drugs, antimicrobial peptides, ligands, growth hormones, antigens, and imaging agents had contributed to the establishment of multifunctional liposomal gel system has significant advantage in regenerative medicine area. This review will focus the advantage of multifunctional liposomal gels in context of infectious wound healing, skin rejuvenation, musculoskeletal repair and trauma management, spinal cord injury treatment, tumor specific chemotherapy as well as immunotherapy and vaccination. The versatility in executing the multiple functions will be a valuable solution for advancing the therapeutic outcomes in regenerative medicine.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"321-333"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}