Journal of Liposome Research最新文献

{"title":"Melatonin hyalurosomes as a powerful antioxidant for combating skin damage induced by UV radiation.","authors":"Mariam Zewail, Haidy Abbas, Merhan E Ali, Shaimaa Makled","doi":"10.1080/08982104.2025.2484732","DOIUrl":"10.1080/08982104.2025.2484732","url":null,"abstract":"<p><p>Extrinsic skin aging is caused by chronic skin photodamage. The present study aims to inspect the role of nanoencapsulation of melatonin (MEL) in hyalurosomes in combating UVB-induced skin damage to take advantage of the hydrating penetration enhancing and antiaging effects of hyaluronic acid along with the powerful antioxidant effects of MEL. Measurement of particle size, zeta potential, encapsulation efficiency and <i>in vitro</i> MEL release were carried out. The <i>in vivo</i> photoprotective effects of MEL were tested in rats. A histopathological examination was conducted, and antioxidant and anti-inflammatory markers were measured along with estimating the expression of P38 MAPK, P-ERK and P-JNK. Particle size and zeta potential of MEL hyalurosomes were 285.9 nm and -26.3 mV with 95% entrapment efficiency and provided a sustained release profile for 48h. <i>In vivo,</i> results revealed the superior effect of MEL hyalurosomes in protecting skin against UVB-induced damage and reducing the levels of inflammatory mediators like TNF-α and IL6 compared with MEL suspension. However, they had a prominent role in increasing the levels of antioxidants. These findings may be accredited to the effect of nanoencapsulation in enhancing skin penetration and deposition of MEL besides the effect of hyaluronic acid as a powerful antiaging tool.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"267-282"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox-responsive liposomes aimed at nitroreductase for contents release.","authors":"Brajadulal Ghosh, Robin L McCarley","doi":"10.1080/08982104.2025.2490537","DOIUrl":"10.1080/08982104.2025.2490537","url":null,"abstract":"<p><p>Here, we report a novel stimuli-responsive N-DOPE liposome where the redox-active 4-nitrobenzyl formate head group of liposomes would respond with respect to the presence of nitroreductase present in the environment of tumor tissues to release the payload. Our main emphasis is related to the construction of redox-sensitive liposomes that would function as the liposomal drug carriers to malignant tumors. Our N-DOPE liposome contains a nitro group (-NO₂) in the modified lipid, and we expect the reduction of the nitro group (-NO₂) to amine (-NH₂) would release the calcein (drug) through the 1,6 elimination as per our hypothesis. But we found no release after waiting for almost 20 hours with the use of Na₂S₂O₄, nitroreductase (NTR) and changes of different external environmental conditions, <i>i.e.</i> temperature, aerobic and anaerobic, etc. due to the formation of an azo (R-N = N-R) bond that stops the complete reduction of (-NO₂) all the way down to form amine (-NH₂) to stop releasing the payloads. However, adding an organic group containing nitro during the reduction process with the Na₂S₂O₄ resulted in a 45% release of liposomal content. A detailed study & explanation of the formation of azo bond in our N-DOPE liposome has been shown in a stepwise manner in through various spectroscopic methods, and we have discussed future directions.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"290-299"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-derived extracellular vesicles: emerging players in hemostasis and thrombosis.","authors":"Aziz Kubaev, Fadhil Faez Sead, Mohammad Pirouzbakht, Mobina Nazari, Hanieh Riyahi, Omolbanin Sargazi Aval, Alireza Hasanvand, Forough Mousavi, Hamed Soleimani Samarkhazan","doi":"10.1080/08982104.2025.2495261","DOIUrl":"10.1080/08982104.2025.2495261","url":null,"abstract":"<p><p>Platelets, long recognized for their role in hemostasis and thrombosis, have emerged as key players in a wide array of physiological and pathological processes through the release of platelet-derived extracellular vesicles (PEVs). These nanoscale vesicles, rich in bioactive molecules such as proteins, lipids, and nucleic acids, facilitate intercellular communication and influence processes ranging from angiogenesis and inflammation to immune modulation and tissue repair. PEVs, the most abundant extracellular vesicles in circulation, display procoagulant activity 50-100 times greater than activated platelets, underscoring their pivotal role in hemostasis and thrombosis. Recent research has unveiled their dual role in health and disease, highlighting their potential as diagnostic biomarkers and therapeutic vehicles. PEVs are implicated in cancer progression, autoimmune diseases, and infectious diseases, where they modulate tumor microenvironments, immune responses, and inflammatory pathways. Moreover, their ability to deliver therapeutic agents with high specificity and biocompatibility positions them as promising tools in regenerative medicine, drug delivery, and targeted therapies. This review comprehensively explores PEV biogenesis, cargo composition, and their multifaceted roles in hemostasis and thrombosis, as well as their broader implications in disease. It also explores the potential of PEVs as diagnostic markers and innovative therapeutic strategies, offering insights into their application in treating thrombotic disorders, cancer, and inflammatory diseases. Despite significant advancements, challenges remain in standardizing isolation protocols and translating preclinical findings into clinical applications. Unlocking the full potential of PEVs promises to revolutionize diagnostics and therapeutics, paving the way for novel approaches to managing complex diseases.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"334-344"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of oral resveratrol-loaded nanoliposomes on hyperlipidemia via toll-like receptor 3 and TIR domain-containing adaptor inducing interferon-β protein expression in an animal model.","authors":"Nasrin Beheshtkhoo, Mohammad Amin Jadidi Kouhbanani, Seyed Mojtaba Daghighi, Maryam Shakouri Nikjeh, Zahra Esmaeili, Masood Khosravani, Mahdi Adabi","doi":"10.1080/08982104.2025.2476529","DOIUrl":"10.1080/08982104.2025.2476529","url":null,"abstract":"<p><p>Hyperlipidemia, a critical risk factor for various health conditions, necessitates innovative therapeutic strategies. Investigating the effectiveness of liposomal formulations in managing hyperlipidemia is essential. Resveratrol (RES)-loaded nanoliposomes present a promising new approach for hyperlipidemia treatment. In this study, we investigated the anti-hyperlipidemic potential of RES-loaded nanoliposomes in high-fat diet (HFD)-fed rats. The nanoliposomes were prepared using a thin-film hydration method. According to transmission electron microscopy (TEM) and dynamic light scattering (DLS) results, the mean size of prepared RES-loaded nanoliposomes were about 42 nm and 68 nm, respectively, with a zeta potential of -65.6 mV. The entrapment efficiency and loading content were 83.78% and 14.25%, respectively. Additionally, the RES-loaded nanoliposomes exhibited controlled release kinetics compared to the free RES form. Moreover, in a hyperlipidemic rat model induced by an HFD, orally administered RES-loaded nanoliposomes significantly reduced total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), and triglycerides (TG), while concurrently increasing high-density lipoprotein cholesterol (HDL-C) levels. Additionally, liver damage induced by HFD was alleviated by RES-loaded nanoliposomes. The expression levels of Toll-like receptor 3 (TLR3) and TIR domain-containing adaptor-inducing interferon-β (TRIF) were assessed using fluorescence immunohistochemistry. Notably, RES-loaded nanoliposomes significantly reduced the expression of these protein. The effect of RES-loaded nanoliposomes was measured on body weight of HFD rats, demonstrting RES-loaded nanoliposomes hold promise for weight management. These findings underscore the potential of RES-loaded nanoliposomes as a safe and effective therapeutic option for hyperlipidemia.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"225-251"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/08982104.2025.2537592","DOIUrl":"https://doi.org/10.1080/08982104.2025.2537592","url":null,"abstract":"","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1"},"PeriodicalIF":3.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomes applied in healing bacterially infected wounds: a systematic review.","authors":"Tainara Aparecida Nunes Ribeiro, Daniel Crístian Ferreira Soares, Grazielle Aparecida Dos Santos, Maria Caiane Lino de Souza, Brenda Xavier Gonçalves, Paula Fernanda da Silva Valentim, Mariana Oliveira de Paula, Daniela Sachs","doi":"10.1080/08982104.2025.2528086","DOIUrl":"10.1080/08982104.2025.2528086","url":null,"abstract":"<p><p>Concerns regarding bacterial infections and antibiotic resistance development have instigated many researchers to synthesize innovative antibacterial compounds and develop more effective pharmaceutical formulations against microorganisms increasingly adapted to our environment. In special, many efforts have been dedicated to developing pharmaceutical antibacterial formulations to treat and improve wound-healing processes, which still represent a great challenge for common clinical practice today. Liposomal systems remain one of the most extensively studied controlled delivery platforms, and considering the antibacterial context, the present article aims to provide a fast and well-oriented systematic review regarding this issue, aiming to bring to the readers the ultimate data regarding the employment of liposomes in the treatment of bacterial infections and the healing of bacteria-infected wounds. A systematic compilation of results and comparisons was made, seeking to observe the antibacterial and wound-healing behavior of the formulated liposomal systems in selected recent studies. The results obtained revealed impressive results concerning the potential applications of liposomes against bacteria in the wound-healing medical context.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-17"},"PeriodicalIF":3.6,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conjugated crown ether lipid liposomes: enhancing integrity in serum through novel surface modifications.","authors":"Athanasios Skouras, Eirini Mallikopoulou, Gerasimos Tsivgoulis, Spyridon Mourtas, Sophia G Antimisiaris","doi":"10.1080/08982104.2025.2530003","DOIUrl":"10.1080/08982104.2025.2530003","url":null,"abstract":"<p><p>This study explores the potential of 18-crown-6 ether to enhance liposomal integrity in biological media. A novel 1,2-Dipalmitoyl-<i>sn</i>-glycero-3-phosphoethanolamine (DPPE)-crown ether conjugate was synthesized and incorporated into phosphatidylcholine/cholesterol liposomes at varying concentrations (0.5-8 mol%). The physicochemical characteristics, serum integrity, and response to divalent cations were systematically evaluated. Although, these crown ether-modified liposomes do not provide the same level of protection as PEGylation at 48 hours, they clearly exhibited improved integrity in serum at 24 hours compared to non-pegylated liposomes under the same conditions. Moreover, when exposed to calcium ions (5 mM), crown ether-modified liposomes maintained significantly greater stability levels at 24 h in comparison to unmodified liposomes. Furthermore, co-functionalization with both crown ether (2%) and PEG (2%) lipids resulted in stability identical to 4% PEGylated liposomes under the same conditions, suggesting an additive stabilization effect of crown ether and PEG, at least at the concentration applied. These findings introduce crown ether-modified liposomes as a novel approach to modulate liposome-protein interactions and potentially enhance drug delivery in physiological environments containing high concentrations of calcium.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-9"},"PeriodicalIF":4.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and immunoactivity of sulfated glucan from <i>Saccharomyces cerevisiae</i> liposomes.","authors":"Yijiong Tao, Linjie Huang, Zhaolong Li, Jiayi Li, Qi Tang, Kai Chen, Lifang Zhang, Chenzhong Fei, Yinchun Liu, Mi Wang","doi":"10.1080/08982104.2025.2527096","DOIUrl":"https://doi.org/10.1080/08982104.2025.2527096","url":null,"abstract":"<p><p>This study optimized the preparation conditions of sulfated glucan from <i>Saccharomyces cerevisiae</i> liposomes (SGSCL) and evaluated its effect on immune activity. SGSCL was prepared using the reverse evaporation method, and its immune activity was assessed by measuring the proliferation of chicken spleen lymphocytes, hemagglutination inhibition (HI) antibody titers, and serum cytokine concentrations in chickens vaccinated with the Newcastle disease (ND) vaccine. The optimal preparation conditions were a phospholipid-to-cholesterol mass ratio of 5.4:1, a phospholipid-to-SGSC mass ratio of 10:1, and a rotary evaporation temperature of 40 °C. The average encapsulation efficiency (EE) was 63.92%, whereas the mean particle size, polymer dispersity index (PDI), and zeta potential were 90.39 ± 1.71 nm, 0.203 ± 0.004, and -41.13 ± 1.05 mV, respectively. SGSCL significantly promoted the proliferation of chicken spleen lymphocytes, splenic T and B lymphocytes at concentrations of 100-800 µg/mL, 800 µg/mL and 200-800 µg/mL <i>in vitro</i>. The best proliferative effect on splenic lymphocytes were at 400 µg/mL, 800 µg/mL, and 800 µg/mL. <i>In vivo</i>, on days 7 and 14, HI antibody titers in the SGSCL-H, SGSCL-M, and SGSCL-L groups were significantly greater than those in the VC group. The serum antibody titers in the SGSCL-H and SGSCL-M groups were significantly or numerically elevated compared to the VC group at all time points post-vaccination. The IL-2, IL-6, IL-4, and IFN-γ concentrations in the SGSCL-H group were significantly higher than that in the VC group on D28 and D35. These findings suggest that SGSCL could serve as a novel vaccine diluent or immune adjuvant.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-12"},"PeriodicalIF":3.6,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved application of the inverse emulsion method for generating flexible asymmetric liposomes for DNA plasmid delivery.","authors":"Denisse Gardea-Gutiérrez, Manuel Román-Aguirre, Berenice E Oseguera-Guerra, Raúl Loera-Valencia, Silvia L Montes-Fonseca","doi":"10.1080/08982104.2025.2521067","DOIUrl":"https://doi.org/10.1080/08982104.2025.2521067","url":null,"abstract":"<p><p>The design of vehicles for transdermal gene delivery is at the forefront of molecular medicine, facilitating targeted therapies. Reports suggest that flexible liposomes can be a good alternative for transdermal delivery, and asymmetric liposomes may enhance gene delivery efficiency. This study aims to create flexible asymmetric-type liposomes with high encapsulation of DNA and high deformability rates. The synthesis of asymmetric liposomes was standardized using the inverse emulsion method, with lipids DOTMA (1,2-di-O-octadecenyl-3-trimethylammonium propane) and DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) as the inner layer, DSPC (1,2-distearoyl-sn-glycero-3-phosphocholine) lipid as the outer layer, cholesterol as a stabilizing component, and Span 80 and ethanol as components that promote flexibility. The pIRES2-EGFP plasmid was used as the encapsulated genetic material. Asymmetric liposomes were characterized using transmission electron microscopy (TEM), encapsulation efficiency percentage (%EE), and the deformability index determined by the extrusion method. Results indicate that the asymmetric liposomes possess a well-defined bilayer, with bilayer deformability varying depending on the components used; for instance, liposomes containing flexible components exhibit a more deformable bilayer than those made solely of lipids. The average size of the liposomes was below 200 nm, and the %EE ranged from 75% to 90%. The liposomes containing Span 80 surfactant exhibited the highest flexibility index. This technique successfully produced asymmetric liposomes with appropriate encapsulation of the DNA plasmid without degradation during the process. Future studies are expected to evaluate the cytotoxicity, transfection, and skin permeation.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-9"},"PeriodicalIF":3.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mito-phytosomal nanocarriers of <i>purslane</i> extract augments apoptosis in A549 cells.","authors":"Hadi Sardarabadi, Seyed Mohammad Zarei, Masoumeh Dolati, Mohammad Hasan Darvishi, Mahdi Tavakolizadeh, Fatemeh Zohrab, Hamidreza Javadi","doi":"10.1080/08982104.2025.2521718","DOIUrl":"https://doi.org/10.1080/08982104.2025.2521718","url":null,"abstract":"<p><p>To enhance the anticancer effects of the purslane extract, we developed a phytosomal nanocarrier with mitochondrial targeting capabilities. Initially, a phytosomal carrier was prepared and subsequently functionalized with a Szeto-Schiller (SS) peptide as, a mitochondrial-penetrating peptide, via a DSPE-PEG (2000)-malamide crosslinker. High-performance liquid chromatography analysis was conducted to quantify the amounts of quercetin and apigenin in the hydroalcoholic extract and the fractionated isolates obtained from diethyl ether, chloroform, ethyl acetate, butanol, and water. The results indicated that both polyphenols were present in the hydroalcoholic extract, with apigenin being more abundant in the ethyl acetate fraction. Dynamic light scattering measurements revealed an average particle size of 112.2 ± 6.758 nm, a narrow polydispersity index of 0.26 ± 0.005, and a zeta potential of -30.67 ± 2.894 mV. Scanning electron microscopy confirmed that the phytosomal carrier exhibited a spherical and intact morphology. The encapsulation efficiency and loading capacity of the phytosomes were found to be 97% and 12.19%, respectively, for the ethyl acetate isolate. The in vitro drug release profile demonstrated a biphasic pattern, characterized by an initial burst release followed by prolonged sustained release. Cytotoxicity assays conducted on A549 and HFF cell lines revealed that the mitochondria-targeted phytosomal nanocarrier exhibited significant apoptotic effects (69.6%) on A549 cells, showing no significant toxicity toward HFF cells when compared with phosphatidylcholine, conventional phytosomal nanocarriers, and ethyl acetate-targeted phytosomal nanocarriers. The findings from this study represent a crucial advancement in the standardization of plant extracts and the development of herbal nanomedicine using targeted phytosomes.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-14"},"PeriodicalIF":3.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}