2007 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第7页

Pluripotent stem cells developed into regenerated tooth by organ germ method in combination with tooth germ-derived epithelium 用器官胚法将多能干细胞与牙胚源性上皮结合，发育成再生牙

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420853

R. Morita, K. Nakao, M. Ogawa, Y. Saji, K. Ishida, T. Tsuji

{"title":"Pluripotent stem cells developed into regenerated tooth by organ germ method in combination with tooth germ-derived epithelium","authors":"R. Morita, K. Nakao, M. Ogawa, Y. Saji, K. Ishida, T. Tsuji","doi":"10.1109/MHS.2007.4420853","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420853","url":null,"abstract":"The regenerative therapy ultimately aims to develop bioengineered organs that can replace lost or damaged organs following disease, injury or aging. Recently, we showed that the artificial organ germ, which generates a structurally correct tooth, could be reconstituted by in vitro cell manipulation (Nature Methods 4, 227-230, 2007). In this study, we revealed that neural crest-like cells, which were differentiated from pluripotent stem cells such as embryonal carcinoma (EC) cells, could develop the regenerated tooth by organ germ method with tooth germ epithelium. EC cells were stimulated with dimethyl sulfoxide (DMSO) and differentiated cells were isolated by cell sorting as DMSO-EC cells. We found that the expressions of pluripotent stem cell marker genes (Oct3/4 and Nanog) could not be detected in DMSO-EC cells. In contrast, the expressions of neural crest-marker genes (Pax3 and Slug) and of dental mesenchyme-marker genes (Msxl, Pax9 and Lhx7) increased in DMSO-EC cells, compared with those in EC cells. Furthermore, the structurally correct tooth can be generated by combining DMSO-EC cells and tooth germ epithelium, both in vitro and in vivo. Our current results indicated the possibility that pluripotent stem cells are applicable as a candidate of cell sources to develop of a bioengineered organ germ for the organ replacement in the future regenerative therapy.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131662439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Caveola ATP synthase mediates ATP release in vascular endothelial cells exposed to shear stress 血管内皮细胞受剪切胁迫时ATP合成酶介导ATP释放

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420887

K. Yamamoto, S. Obi, N. Shimizu, S. Kumagaya, J. Ando

{"title":"Caveola ATP synthase mediates ATP release in vascular endothelial cells exposed to shear stress","authors":"K. Yamamoto, S. Obi, N. Shimizu, S. Kumagaya, J. Ando","doi":"10.1109/MHS.2007.4420887","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420887","url":null,"abstract":"Endothelial cells (ECs) release ATP in response to shear stress, a mechanical force generated by blood flow, and the ATP released modulates EC functions through activation of purinoceptors. The molecular mechanism of the shear-stress-induced ATP release, however, has not been fully understood. In this study, we have demonstrated that cell-surface ATP synthase is involved in shear stress-induced ATP release. Immunofluorescence staining of human pulmonary artery ECs (HPAECs) showed that cell-surface ATP synthase is distributed in lipid rafts and co-localized with caveolin-1. When exposed to shear stress, HPAECs released ATP in a dose-dependent manner, and the ATP release was markedly suppressed by a membrane-impermeable ATP synthase inhibitor, angiostatin, and by an anti-ATP synthase antibody. Depletion of plasma membrane cholesterol with methyl-beta cyclo-dextrin (MbetaCD) disrupted lipid rafts and abolished co-localization of ATP synthase with caveolin-1, which resulted in a marked reduction in shear-stress-induced ATP release. Down-regulation of caveolin-1 expression by transfection of caveolin-1 siRNA also markedly suppressed ATP-releasing responses to shear stress. These results suggest that the localization and targeting of ATP synthase to caveolae/lipid rafts, is critical for shear stress-induced ATP release by HPAECs.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131070419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of 'Promoter Chip' for Microarray Analysis of Regulation Targets of Transcription Factors 转录因子调控靶点微阵列分析“启动子芯片”的构建

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420839

K. Yamamoto, A. Ishihama

{"title":"Construction of 'Promoter Chip' for Microarray Analysis of Regulation Targets of Transcription Factors","authors":"K. Yamamoto, A. Ishihama","doi":"10.1109/MHS.2007.4420839","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420839","url":null,"abstract":"Escherichia coli carries a sophisticated genetic system for adaptation to changes in external environment by selective transcription of a total of about 4,400 genes on its genome. Gene selection takes place at the step of promoter recognition by the RNA polymerase. The promoter selectivity of RNA polymerase is, however, modulated after interaction with DNA-binding transcription factors. In E. coli, a total of about 300 species of transcription factor have been predicted, even though the regulatory functions remain identified for about half of these factors. In order to understand the global regulation of genome expression in E. coli, it is essential to identify the regulatory functions of all 300 transcription factors. For quick search of the regulation targets by each transcription factor, we developed the genomic SELEX (see Ishihama et al., in this volume). In parallel, we have constructed a novel method for comprehensive search of the promoters under the control of each transcription factor. Here we have constructed a 'promoter chip', which contains more than 1,000 species of the E. coli promoters. The promoter microarray can be used for identification of target promoters regulated by each transcription factor.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126792762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Towards constructing synthetic cells: RNA/RNP evolution and cell-free translational systems in giant liposomes 构建合成细胞:巨大脂质体中的RNA/RNP进化和无细胞翻译系统

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420868

H. Saito, A. Yamada, R. Ohmori, Y. Kato, T. Yamanaka, K. Yoshikawa, T. Inoue

{"title":"Towards constructing synthetic cells: RNA/RNP evolution and cell-free translational systems in giant liposomes","authors":"H. Saito, A. Yamada, R. Ohmori, Y. Kato, T. Yamanaka, K. Yoshikawa, T. Inoue","doi":"10.1109/MHS.2007.4420868","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420868","url":null,"abstract":"The emerging field of synthetic biology seems to have a great potential to the development of new biotechnologies and the understanding of self-organizing principle in life. Currently, several synthetic biologists aim to understand the evolution of naturally occurring biological systems through a \"bottom-up approach\" in contrast to the conventional reductive approach. Several lines of pioneering works have been demonstrated in that behaviors of programmed synthetic networks are predicted in vitro. However, it is crucial to create new molecular \"parts\" for (re)constructing synthetic genetic networks in artificial cell-like compartments, such as giant liposomes. We focus on RNA and RNP (ribonucleoprotein) that hold promise as new \"parts\" for synthetic biology. They are constructed with molecular design and an experimental evolution technique. So far, designed self-folding RNAs, RNA (RNP) enzymes, and nanoscale RNA architectures have been successfully constructed by utilizing Watson-Crick base-pairs together with specific RNA-RNA or RNA-protein binding motifs of known defined 3D structures. In addition, we also demonstrated that the cell-sized liposomes can be prepared by using phospholipid-coated micro-droplets, which were generated by emulsification or microfluidic techniques. We encapsulated cell-free translation system (PURE SYSTEM) in newly developed liposomes, and successfully monitored protein expression kinetics within individual liposomes in real time. By combining RNA/protein in vitro evolution techniques with giant liposome-based systems, it may be possible to generate artificial protocells with translational regulatory systems.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121913231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

High-speed and uniform deposition of amorphous carbon on inner surface of metal tube with microwave-excited high-density plasma column 微波激发高密度等离子体柱在金属管内表面高速均匀沉积非晶碳

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420904

H. Kousaka, S. Kishine, N. Umehara

引用次数: 3

Single-molecule imaging, force measurement and fluorescence observation reveal protein and chromosome dynamics around the nuclear envelope 单分子成像、力测量和荧光观察揭示了核膜周围的蛋白质和染色体动力学

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420872

S. Otsuka, Yasuhiro Hirano, Hirohide Takahashi, Masahiro Kumeta, K. Takeyasu, S. Yoshimura

引用次数: 0

Fabrication of Coil Lines with High Aspect Ratio for Electromagnetic Actuators 电磁执行器高纵横比线圈线的制造

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420895

D. Noda, Y. Matsumoto, M. Setomoto, T. Hattori

{"title":"Fabrication of Coil Lines with High Aspect Ratio for Electromagnetic Actuators","authors":"D. Noda, Y. Matsumoto, M. Setomoto, T. Hattori","doi":"10.1109/MHS.2007.4420895","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420895","url":null,"abstract":"Actuators are finding increasing use in the various fields. And, it is one of most important parts for machines because it determines its performance. Actuators account for a large percentage and volume and weight of a product compared with other parts. Therefore, the progress in downsizing of actuators was required. Then, we have proposed a high aspect ratio coil lines for electromagnetic actuators in order to expect to reduce the size and increase the output power. To realize these coils and actuators, we have fabricated a three dimensional X-ray lithography and metallization process. Using these processes, we have formed three-dimensional coil lines with high aspect ratio that could be used in electromagnetic actuators. In this paper, we have fabricated coil lines with 10 mum line width, 20 mum pitch and aspect ratio of 5 on the surface of an acrylic pipe by mean of three-dimensional X-ray lithography technique. And, a void-free metallic deposit could be formed on a thin coil lines by electroplating method. It is very expected the high performance microcoils and actuators with high aspect ratio could be manufactured in spite of the miniature size.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115034237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Wall Climbing Mechanisms Using Electrostatic Attraction Generated by Flexible Electrodes 利用柔性电极产生静电吸引的爬墙机构

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420886

A. Yamamoto, T. Nakashima, T. Higuchi

引用次数: 98

Magnetically Modified Soft Micro Actuators for Oocyte Manipulation 用于卵母细胞操作的磁改良软微致动器

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420896

Y. Yamanishi, S. Sakuma, F. Aral

引用次数: 25

Improvement of the low speed controllability of a V-shaped, two bolt-clamped Langevin-type transducer, ultrasonic linear motor 改进了一个v形、两个螺栓夹紧的朗格万型换能器、超声波直线电机的低速可控性

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420884

K. Asum, T. Fujimura, R. Fukunaga, M. Kurosawa

引用次数: 9