2007 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第8页

Sensing Temperature in Water using FIB CVD Nano Thermal Sensors 利用FIB CVD纳米热传感器感应水中温度

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420817

H. ElShimy, M. Nakajima, F. Arai, Toshio Fukuda

{"title":"Sensing Temperature in Water using FIB CVD Nano Thermal Sensors","authors":"H. ElShimy, M. Nakajima, F. Arai, Toshio Fukuda","doi":"10.1109/MHS.2007.4420817","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420817","url":null,"abstract":"In this paper we illustrate the fabrication process of nano temperature sensor probe using focused ion beam chemical vapor deposition (FIB-CVD) of tungsten over atomic force microscope (AFM) cantilevers, to be used in sensing temperature distribution in local area. We present the fabrication approach & modifications for making this sensor probe capable of sensing temperature distributions not only in air but in water environment as well. The nano sensor probe was calibrated in water using the hot stage of the environmental scanning electron microscope (ESEM). The experimental results show the positive characteristics of the temperature coefficient of resistance (TCR). We also illustrate the response of the sensor to sudden changes in the surrounding medium. The characteristics of this sensor probe were compared to previously reported temperature sensing devices. The comparison verifies that our sensor is relatively uncomplicated and reliable in fabrication. The capability of sensing temperature in water will allow our sensor to be used in wide range of bio-applications, especially in studying thermogenesis in single cells.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"229 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116380541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Photo-Induced DNA Double-Strand Breaks Monitored by Single-Molecule Observation: Protective Effect of Antioxidative Compounds 单分子观察监测光诱导DNA双链断裂:抗氧化化合物的保护作用

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420866

Yuko Yoshikawa, Etsuko Hirose, Emi Sakai, Chiharu Ikawa

引用次数: 0

Complex Formations between Artificial RNA-DNA Chimera Nucleic Acids and RNA Modification Enzyme 人工RNA- dna嵌合体核酸与RNA修饰酶的复杂结构

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420836

A. Ochi, H. Hori

{"title":"Complex Formations between Artificial RNA-DNA Chimera Nucleic Acids and RNA Modification Enzyme","authors":"A. Ochi, H. Hori","doi":"10.1109/MHS.2007.4420836","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420836","url":null,"abstract":"In living cells, RNA modification enzymes form multiple styles of RNA-protein complexes with various precursor RNA species for generation of mature RNA molecules. In general, however, preparation of the processing-modification complex is very difficult, because the complex is dissociated according to progress of the enzymatic reactions. Until now, several ways to prevent the dissociation of complexes have been devised, for examples utilization of small substrate analogues and/or disruption of the catalytic center of the enzyme. However, in the case of some RNA methyltransferases such as tRNA (Gml8) methyltransferase [TrmH], these approaches are not successful. TrmH enhances the affinity for RNA only in the presence of the methyl group donor, S-adenosyl-L-methionine (AdoMet). AdoMet analogues do not work like AdoMet in the case of TrmH, because TrmH requires the activated methyl group in AdoMet for structural change of the enzyme. Furthermore, disruptions of the catalytic center (Arg41 residue) by amino acid substitutions cause perturbation of the local protein structure around the catalytic center and result in decrease of the affinity for RNA. Therefore, a new approach for stabilization of the RNA-protein complex is desired. In the current study, we prepared artificial RNA-DNA chimera nucleic acids, in which ribonucleotide(s) is substituted by deoxyribonucleotide(s). Some of the artificial nucleic acids formed stable complexes with TrmH.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128394772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Development of Multi-Layer Scaffolds Based on Artificial Configuration" 基于人工构型的多层支架的研制

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420845

H. Oura, T. Uchida, S. Ikeda, T. Nakano, F. Arai, M. Negoro, T. Matsuda, T. Fukuda

{"title":"\"Development of Multi-Layer Scaffolds Based on Artificial Configuration\"","authors":"H. Oura, T. Uchida, S. Ikeda, T. Nakano, F. Arai, M. Negoro, T. Matsuda, T. Fukuda","doi":"10.1109/MHS.2007.4420845","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420845","url":null,"abstract":"In this work, we propose a multi-layered scaffold made of elastic biodegradable polymer PLCL [1] by salt leaching method [2], which has high porosity at inner layer and high stiffness in outer layer. Before implanting to a damaged position of a patient, scaffolds should be coated with human umbilical vein endothelial cells (HUVECs). (Porous structure is also served as a pathway of sufficient nutrients and oxygen). This inner layer has porosity as means to culture HUVECs on itself, and outer layer has stiffness as means to maintain shape of scaffolds against blood flow as that of blood vessels does. These inner and outer layers were reproduced also as means of intimal layer and internal elastic lamina in human blood vessel especially muscular artery respectively. It was also verified that this fabrication method can be applied to fabricate tri-layered tubular artificial blood vessel scaffold by SEM. Mechanical characterization and porosity of bi-layered scaffolds were evaluated by tensile test and image processing respectively. These parameters were confirmed that can be controllable by adjusting concentration of NaCl for inner layer used in salt leaching method. HUVECs were cultured on the porous structure of bi-layered scaffolds, and the scaffold's biocompatibility was confirmed by viability of cells. Results obtained from these experiments shows we can propose artificial blood vessel scaffold, which has optimal porosity and mechanical strength, not to induce blood clot, intimal hypertrophy and so on in patient's body.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133864880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of Artificial System of Exocytotic Cells 外泌细胞人工系统的研究进展

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420837

M. Sasai, H. Sakiyama, S. Tadokoro, N. Hiroshima

{"title":"Development of Artificial System of Exocytotic Cells","authors":"M. Sasai, H. Sakiyama, S. Tadokoro, N. Hiroshima","doi":"10.1109/MHS.2007.4420837","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420837","url":null,"abstract":"Regulated exocytosis is a very important process to secrete bioactive substances from cells, such as neurotransimitters, hormones, and inflammatory mediators from immune cells. Secretory cells have intracellular small vesicles called secretory vesicles or secretory granules and they contain bioactive substances. These contents are released by membrane fusion between secretory vesicles and the plasma membrane (exocytosis). Mast cells are typical non-neuronal secretory cells and secrete inflammatory mediators such as histamine. It has been shown that SNARE (soluble N-ethyl maleimide-sensitive factor attachment protein receptor) proteins play an essential role in exocytotic release in mast cells. In this study, we tried to develop an artificial system that mimics exocytotic release of mast cells, using liposomes in which purified SNARE proteins are reconstituted. To develop a such system, we try to prepare a cell-size giant liposome that contains smaller liposomes. A giant liposome and smaller liposomes correspond to the cell membrane and secretory vesicles, respectively. Preparing giant liposomes, LUVs that contain smaller liposomes inside like mast cells were formed. Diameter of inner liposomes ranged from 1 to 3 mum and the number of inner liposomes ranged from 1 to 20. We also succeeded preparing giant liposomes that contain calcein-labeled SUV by adding SUV at the process of rehydration. These system might be useful to construct an artificial exocytotic cells.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130989357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Passive Operating On-chip Plasma Isolation From Whole Blood 全血被动操作芯片血浆分离

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420856

Z. Meng, F. Aita, S. Khumpuang, K. Ooe, S. Sugiyama, K. Miyamura, M. Ikeda, H. Yonezawa, Y. Ohmori, Matsumoto

引用次数: 0

Grip Force Control Based on the Degree of Slippage Using Optical Tactile Sensor 基于滑动度的光学触觉传感器握持力控制

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420900

Norinao Watanabe, G. Obinata

{"title":"Grip Force Control Based on the Degree of Slippage Using Optical Tactile Sensor","authors":"Norinao Watanabe, G. Obinata","doi":"10.1109/MHS.2007.4420900","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420900","url":null,"abstract":"Recently, many kinds of tactile sensors for robot gripper have been proposed. One of the problems of these sensors for dexterous handling is to requiring information of grasping object such as the weight and the friction coefficient. They are difficult to be measured before grasping. In this paper, we propose a control method for robot gripper not using the friction coefficient. For this purpose, we use a vision-based tactile sensor which consists of a CCD camera, LED lights, acrylic plate and spherical elastic body. The feature of this sensor is measuring \"stick ratio\", which shows the degree of slippage between the robot gripper and the grasping object. When an elastic body contacts a rigid body and slides slightly, two regions, called \"stick region\" and \"incipient slip region\", appear in the contact area. The stick ratio is the ratio of the area of incipient slip region to that of the contact area. We propose the control system for robot gripper to achieve dexterous handling using the stick ratio. The stick ratio measured by the tactile sensor is used to control the grip strength. As a result, macroscopic slippage is prevented by control. It is experimentally showed that our control method for robot gripper makes it possible to achieve dexterous handling only using signals from the tactile sensor.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116149657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Single-molecule Observation of DNA Hydrolysis by ExonucleaseIII; Effect of Physical Form of DNA on Exonuclease Reaction 外切酶eiii水解DNA的单分子观察DNA物理形态对外切酶反应的影响

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420842

H. Kurita, K. Torii, H. Yasuda, K. Takashima, S. Katsura, A. Mizuno

引用次数: 0

Ligation of DNA Based on Single-Molecule Manipulation 基于单分子操作的DNA连接

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420831

R. Watanabe, H. Oana, M. Washizu

引用次数: 0

In-situ Single Cell Mechanics Characterization of W303 Yeast Cells using Nanoneedles inside Environmental-SEM 环境扫描电镜下纳米针对W303酵母细胞单细胞力学特性的原位表征

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420816

M. Ahmad, M. Nakajima, S. Kojima, M. Homma, T. Fukuda

{"title":"In-situ Single Cell Mechanics Characterization of W303 Yeast Cells using Nanoneedles inside Environmental-SEM","authors":"M. Ahmad, M. Nakajima, S. Kojima, M. Homma, T. Fukuda","doi":"10.1109/MHS.2007.4420816","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420816","url":null,"abstract":"In this paper, the characterizations of cellular mechanics of W303 yeast cells have been conducted using nanoneedles inside environmental-SEM (ESEM). This enhanced ESEM system comprises of a standard ESEM instrument as a nano imaging tool, a cooling stage as a cellular biology's humidity controller and 7 D.O.F. linear actuators as nanomanipulator/effector. Four types of nanoneedles have been used in the experiments, i.e. silicon (Si) nanoneedle, titanium (Ti) coated Si nanoneedle and tungsten (W) nanoneedles. The former two nanoneedles are fabricated using 2 N/m spring constant's cantilevers. While the latter nanoneedles are fabricated using 0.09 N/m and 2 N/m spring constant's cantilevers (W0.09 and W2 nanoneedles). All types of the nanoneedles, i.e. Si, Ti-Si, W0.09 and W2 nanoneedles are able to be used for single cells' local stiffness characterizations. This ability can be used in future fast disease detection where the disease cells may shows different cell mechanics properties compare to the normal cell. In addition to this, the Si-Ti and W2 nanoneedles have shown an ability to penetrate the cell without cell bursting. This ability is important in the future single cell surgery where the cell damage can be eliminated as much as possible.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128727143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1