2007 International Symposium on Micro-NanoMechatronics and Human Science最新文献

Two-photon microfabrication with a supercritical CO2 drying process toward replication of three-dimensional microstructures 用超临界CO2干燥工艺制备三维微结构的双光子微加工

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-12-01 DOI: 10.1109/MHS.2007.4420818

T. Hasegawa, S. Maruo

引用次数: 4

Cationic Silica Nanoparticles are Efficiently Transferred into Mammalian Cells 阳离子二氧化硅纳米颗粒有效转移到哺乳动物细胞

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-12-01 DOI: 10.1109/MHS.2007.4420867

Li Liu, T. Takenaka, A.A. Zinchenlco, N. Chen, S. Inagaki, H. Asada, T. Kishida, O. Mazda, S. Murata, K. Yoshikawa

{"title":"Cationic Silica Nanoparticles are Efficiently Transferred into Mammalian Cells","authors":"Li Liu, T. Takenaka, A.A. Zinchenlco, N. Chen, S. Inagaki, H. Asada, T. Kishida, O. Mazda, S. Murata, K. Yoshikawa","doi":"10.1109/MHS.2007.4420867","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420867","url":null,"abstract":"Nanoparticles, as nonviral vectors, are expected as a candidate on highly effective non-viral gene carrier. We prepared organically modified cationic silica nanoparticles toward the high performance nonviral vector. It is to be noted that the complexes of DNA and nanoparticles are regarded as a simple model of chromatin. Furthermore, it is reported that the transcription activity of DNA is preserved after complexing with small nanoparticles, where the manner of complex formation is strongly dependent on the size of the nanoparticles in vitro. On the other hand, it is confirmed that transcription is completely inhibited in the compact globule state of giant DNA induced by polyamines and related polycations. The future goal of our undergoing studies is to make clear whether nanoparticles can be used for regulation of the higher-order structure of genomic DNA, and hence activate or inhibit transcription. In the present study, we performed experiments of transfecting nanoparticles into cell. Observation by fluorescence confocal microscopy shows that nanoparticles are effectively taken up in both cytoplasm and nucleus in cells. Importantly, it is also demonstrated that the nanoparticles are non-toxic for cell. The difference in the scenario of nanoparticles uptake by cells has been also observed. The results suggest that organically modified cationic silica nanoparticles may be the next new class of DNA binders and carries for biological and biomedical applications in the future.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116348270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Single cell manipulation by using tilt controlled optical tweezers 单细胞操作使用倾斜控制光学镊子

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-12-01 DOI: 10.1109/MHS.2007.4420873

M. Ichikawa, K. Kubo, S. Murata, K. Yoshikawa, Y. Kimura

引用次数: 0

Fabrication of Cell-Adhesion Surface and Capillary Vessel Model by Photolithography 光刻技术制备细胞粘附表面及毛细血管模型

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420879

T. Nakano, M. Tada, Yu-Ching Lin, S. Ikeda, T. Uchida, H. Oura, T. Fukuda, T. Matsuda, M. Negoro, F. Arai

{"title":"Fabrication of Cell-Adhesion Surface and Capillary Vessel Model by Photolithography","authors":"T. Nakano, M. Tada, Yu-Ching Lin, S. Ikeda, T. Uchida, H. Oura, T. Fukuda, T. Matsuda, M. Negoro, F. Arai","doi":"10.1109/MHS.2007.4420879","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420879","url":null,"abstract":"We have been developing scaffolds of three-dimensional (3D) synthetic vascular prosthesis in tailor-made. Human umbilical vein endothelial cells (HUVECs) attached on the inner surface of the scaffold have anticoagulant effects. Asperity structures of the inner surface are important to cell adhesion. It is important to quantify the inner surface asperity condition of the scaffold by observing HUVECs behavior and morphology. For this purpose, we recreated the inner surface profile of the scaffold on a poly(dimethilsiloxane) (PDMS) substrate by microfabrication. We made semiround convex patterns of resist that had 8 mum in diameter and 5 mum high using photolithography, and the concave pattern on the PDMS substrate by printing. We observed HUVECs adhering to the PDMS substrate having concave pattern on it surface. The distribution density of the concaves of the tested pattern is 1600 /mm2 or 40,000 in a 25 mm2 area. In addition, we fabricated a capillary vessel model by photolithography, creating a branched capillary tube model that had 13 mum in diameter. We confirmed that the capillary vessel model had no leakage using a methylene blue solution flow in the channel.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117214586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Gradient Generation with Active Mixing by a Novel Microvalve using Tailor-made Multilayer Piezoelectric Actuator (TAMPA) 基于定制多层压电致动器的新型微阀主动混合梯度生成

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420857

K. Sekiyama, K. Motoo, F. Arai, T. Fukuda, M. Nakajima

引用次数: 0

Measurement of Young's modulus of primary cilia by using optical tweezers 用光镊测量初级纤毛的杨氏模量

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420852

Y. Harada, T. Ota, T. Takamatsu

{"title":"Measurement of Young's modulus of primary cilia by using optical tweezers","authors":"Y. Harada, T. Ota, T. Takamatsu","doi":"10.1109/MHS.2007.4420852","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420852","url":null,"abstract":"Most renal tubular epithelial cells possess primary cilia that protrude into the tubular lumen, where they are exposed to urine flow. The cilia of the epithelia curve with the urine flow within the renal tubules. The primary cilium of renal epithelium has been assumed to be a mechano-sensor of the urine flow. However, the mechanical characteristics of the cilium of an each cell are not well assessed. In this study, we measured Young's modulus of each primary cilium by using optical tweezers. First, we developed a system to manipulate each primary cilium of living tubular epithelial cells in situ by using optical tweezers. We adopted flexible-substratum technique to visualize individual micron-sized primary cilia of the Madin-Darby canine kidney (MDCK) cell lines. Through the use of the flexible-substratum technique, the primary cilia of MDCK cells could be clearly imaged from the side. We showed that we could apply minute force to polystyrene microspheres stuck on the tip of the cilia by using optical tweezers. The Young's modulus of each primary cilium was successfully examined by measuring the displacement of the microsphere. We concluded that this methodology enables us to manipulate each primary cilium minutely and to estimate the elasticity of an individual primary cilium.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123558619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dynamic Viscoelastic Properties of Confined Polymer Liquids Under Oscillatory Shear Flow 振荡剪切流动下受限聚合物液体的动态粘弹性特性

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420920

S. Itoh, K. Fukuzawa, Y. Hamamoto, Hedong Zhang

引用次数: 0

Application of ultra thin hydrophilic polymer layer to acceleration of cell sheet recovery from temperature-responsive culture dishes 超薄亲水性聚合物层在加速温度响应培养皿细胞片回收中的应用

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420847

Y. Akiyama, M. Yamato, A. Kikuchi, T. Okano

引用次数: 1

Effect of nitrogen atoms included in CNx coatings on friction sliding against Si3N4 ball in nitrogen gas CNx涂层中含氮原子对氮化硅球在氮气中摩擦滑动的影响

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420921

T. Tokoroyama, N. Umehara

{"title":"Effect of nitrogen atoms included in CNx coatings on friction sliding against Si3N4 ball in nitrogen gas","authors":"T. Tokoroyama, N. Umehara","doi":"10.1109/MHS.2007.4420921","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420921","url":null,"abstract":"Carbon nitride (CNx) coatings are thought to have good tribological properties because of low friction and high wear resistance. This material shows a friction coefficient lower than 0.01 when slid against a Si3N4 ball in dry N2 gas. The mechanism of superlow friction was believed to be due to the change in the topmost layer of the CNx surface in the wear track to a low-shear-strength layer by forming a graphite-like structure. However, the effects of nitrogen atoms in the CNx coating and the ambient N2 gas on the transformation of the surface layers of CNx remain unclear. In this work, we investigated the relationship between the friction coefficient and the nitrogen concentration in the wear track on the CNx coating. We measured the intensity of C KLL and N KLL on the wear track of CNx by Auger electron spectroscopy (AES), and conducted Raman analysis. We compared the N KLL/C KLL ratios of wear tracks from sliding in ambient N2, Ar, and O2. Only in the case of the wear track from material slid in N2, did the N KLL/C KLL ratio decrease to almost zero and the friction coefficient became lower than 0.01. On the other hand, in the Ar environment, the N KLL/C KLL ratio in the wear track was comparable to the as-deposited CNx coating, the friction coefficient became 0.03, and the IG position of Raman shift increased at most 3 cm-1. The N KLL/C KLL intensity of the wear track after friction tests in Ar and then in N2 was approximately 0.2 times as low as the as-deposited CNx. The friction coefficient in N2 gas was 0.03 when incorporated nitrogen remained in the CNx surface. On the other hand, the friction coefficient was lower than 0.03 when incorporated nitrogen was desorbed.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122327791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Self-Deployment Algorithm of Mobile Sensor Network based on Connection Priority Criteria 基于连接优先级标准的移动传感器网络自部署算法

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420917

J. Takahashi, K. Sekiyama, T. Fukuda

{"title":"Self-Deployment Algorithm of Mobile Sensor Network based on Connection Priority Criteria","authors":"J. Takahashi, K. Sekiyama, T. Fukuda","doi":"10.1109/MHS.2007.4420917","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420917","url":null,"abstract":"This paper deals with a self-deployment of the mobile sensor network in an unknown environment. A mobile sensor network is composed of a distributed collection of nodes, each of which has sensing, computation, communication and locomotion capabilities. Such a network is capable of self- reconfiguration of the network topology in order to gather distributed information on the dynamic environment. In this paper, we present a single node movement algorithm based on connection priority for node deployment. Each node moves only with local information such that the network accomplishes a global purpose, e.g., transmitting remote sensing data to the sink. Each node in this network transmits a control packet called \"hello message\". Because the hello message may hardly press a communication resource, it is an advantage that this each node uses only hello messages and sensing data in order to decide the moving direction. Suggest that proposed algorithm is directly applicable. Its directly applicable to mobile sensor network for searching injured persons in disaster areas, data gathering in dangerous environments, etc.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"187 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122672315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3