2007 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第2页

Influenza virus selects cell phase for infection 流感病毒选择细胞期进行感染

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420821

R. Ueda, A. Ichkawa, M. Kusunoki, M. Ejima, F. Aral, T. Fukuda, A. Honda

{"title":"Influenza virus selects cell phase for infection","authors":"R. Ueda, A. Ichkawa, M. Kusunoki, M. Ejima, F. Aral, T. Fukuda, A. Honda","doi":"10.1109/MHS.2007.4420821","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420821","url":null,"abstract":"Influenza virus attaches on the sialic acid on cellular membrane through it's HA protein and enters cytoplasm by receptor-mediated endocytosis, then move from endocytic vesicles to early endosomes and late endosome where the influenza viruses fuse with the endosomal membrane to release viral genome followed to transport the genome to nuclei where virus replicate their genome. Previously we found that host cell protein Ebpl (ErbB3 binding protein 1) interacted with the catalytic subunit PB1 of influenza virus RNA dependent RNA polymerase and interfered with its function. Ebpl expression is regulated by cell cycle, showing high level expression especially in Gl to S phase. Recently we identified another host protein (PA4c9) interacts with subunit PA of influenza virus RNA polymerase. Again PA4c9 is one of the cell cycle dependent proteins. These findings encouraged us to investigate the host cell cycle dependency of influenza infection. To identify the cell phase at which influenza virus attach most efficiently, the cells were treated with the fluorescent-labeled influenza viruses for 5-15 minutes and fixed with paraformaldehyde followed by observation under the confocal microscope. In parallel, attempts were made to manipulate a single particle of fluorescent-labeled virus using laser manipulator for infection onto a single cell at different phase. The results altogether indicated that influenza virus preferred the membrane of stable cell phase (G0/G1) to attach for infection.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128457447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and Biochemical Analyses of Zea mays Cytochrome b561 Heterologously Expressed in Pichia pastoris 毕赤酵母中异源表达的玉米细胞色素b561的纯化及生化分析

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420835

M.M. Rahman, N. Nakanishi, T. Takigami, T. Hase, Sam-Yong Park, M. Tsubaki

{"title":"Purification and Biochemical Analyses of Zea mays Cytochrome b561 Heterologously Expressed in Pichia pastoris","authors":"M.M. Rahman, N. Nakanishi, T. Takigami, T. Hase, Sam-Yong Park, M. Tsubaki","doi":"10.1109/MHS.2007.4420835","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420835","url":null,"abstract":"Cytochromes b 561 constitute a novel class of transmembrane electron transport proteins present in large variety of eukaryotic cells, with a number of highly relevant common structural features including the six hydrophobic transmembrane alpha-helices and the two heme ligation sites. Of particular interest is the presence of a number of plant homologues that encode proteins having possible ascorbate- and monodehydroascorbate radical-binding sites proposed previously for mammalian cytochromes b 561. In the present study, we conducted a molecular cloning of cytochrome b 561 cDNA from corn plant, Zea mays, its functional heterologous expression in yeast Pichia pastoris, its purification, and its biochemical analyses. The purified recombinant Zea mays cytochrome b 561 protein (WTZMb561-H6) showed characteristic visible absorption peaks very similar to those of bovine cytochrome b561. The results from a stopped-flow analysis indicated that Zea mays cytochrome b561 utilizes ascorbate and, possibly, monodehydroascorbate radical as a physiological electron donor and acceptor, respectively. Pre-treatment of the purified Zea mays cytochrome b561 with diethylpyrocarbonate in the oxidized form caused a drastic inhibition of the electron transfer from ascorbate and such inhibition was protected by the presence of ascorbate during the treatment with diethylpyrocarbonate. These results suggested that plant cytochrome b561 might perform an ascorbate-related transmembrane electron transfer reaction by utilizing a very similar molecular mechanism with that of bovine cytochrome b561. Our new system offers an improvement in yield and other advantages over existing insect and yeast cell systems for producing the recombinant cytochrome b561 for the studies on structure and functions.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121152877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A Jumping Robot based on the Closed Elastica 基于闭合弹性体的跳跃机器人

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420924

A. Yamada, M. Watari, H. Mochiyama, H. Fujimoto

引用次数: 5

Model-based Robust Online Fault Detection for Mating Process of Electric Connectors in Robotic Wiring Harness Assembly Systems 基于模型的机器人线束装配系统电连接器配合过程鲁棒在线故障检测

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420916

Man Huang, P. Di, T. Fukuda, T. Matsuno

引用次数: 4

Analysis on Kinematics and Statics of Redundant Parallel Link Manipulators with Passive Joints 具有被动关节的冗余并联连杆机械臂运动学与静力学分析

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420901

Y. Imai, G. Obinata, K. Hase

引用次数: 2

Tissue Architecture based on Robotics Manipulation and Functional Artificial Extracellular Matrix 基于机器人操作和功能性人工细胞外基质的组织结构

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420882

D. Kawahara, T. Matsuda

引用次数: 0

Examination of bacterial toxin measurement using Quartz crystal microbalance method 石英晶体微天平法测定细菌毒素的检验

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420860

A. Tsuji, K. Ooe, N. Nishishita, Y. Hirano

引用次数: 0

MEMS technology for Artificial Cells 人造细胞的MEMS技术

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420865

S. Takeuchi

引用次数: 1

Darwinism for MEMS 达尔文主义的MEMS

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420908

J. Korvink, Zhenyu Liu

引用次数: 2

Basic Characteristics of a Multi-pole Spherical Synchronous Motor 多极球面同步电动机的基本特性

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420885

T. Yano, Y. Kubota, T. Shikayama, T. Suzuki

{"title":"Basic Characteristics of a Multi-pole Spherical Synchronous Motor","authors":"T. Yano, Y. Kubota, T. Shikayama, T. Suzuki","doi":"10.1109/MHS.2007.4420885","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420885","url":null,"abstract":"A multi pole spherical synchronous motor is developed and tested. The developed motor has a rotor supported by a gymbal mechanism and a stator with two pairs of armature poles. 260 Small permanent magnets are attached on the rotor surface so that the North poles and the South poles appear on the concentric circles alternatively. Four armature winding units are positioned on the stator. Each armature winding unit has three windings. The pitch of the windings is 2/3 of the pitch of permanent magnets. Therefore, locally, the relation between the armature windings and the permanent magnets is almost the same as that of the conventional multi pole synchronous motor. The motor size is 265 times 265 times 266 [mm] and the diameter of the rotor is 130 [mm]. The target maximum output torque is 0.49 [Nm], The motor has two degrees of freedom and the working area is plusmn45 [deg] around x and y axis. The rotor can rotates around x and y axis and also moves in the direction combining two rotations simultaneously. The motor is driven by two pairs of three phase sinusoidal currents, each amplitude is calculated at the DSP board. The repeated positioning accuracy, the moving speed, and the output torque of the vertical position were tested. The maximum repeated positioning error was 0.00348 [deg]. The maximum moving speed was more than 90 [deg/sec], and the output torque is more than 0.59 [Nm]. Additional trajectory control results also showed small positioning errors.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126271201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18