Examination of bacterial toxin measurement using Quartz crystal microbalance method

Abstract

When renal failure patients undergo the hemodialysis, the endtoxin (ET) that is the bacterial toxin that causes the symptom such as generation of heat is measured as one of the pollution markers. Presently, because the high performance filtration equipment develops, the amount of ET in dyalysate water and solution was achieved under the ET standard value set by Japanese Society for Dialysis Therapy etc. However, the ET measurement is obligated to the moon a couple of degrees at the dialysis center etc. the measurement is needed for 30 minutes or more since it use batch method and rely on the chemical reaction. In this study, we paid attention to the polyamino acid named epsiv-polylisine (epsiv-PL) that can select and adsorb ET and the mass measurement using quartz crystal microbalance method (QCM). We aim to the development of the real-time ET measuring system using these. If it is achieved, the amount of ET can be monitored while blood is dialyzed. Moreover, it seems that treatment that discontinues dialyzing can be taken when it exceeds the reference value.