2007 International Symposium on Micro-NanoMechatronics and Human Science最新文献

{"title":"Design, preparation and directional insertion of peptides into lipid bilayer membrane and their application for the preparation of liposome of which surface could be coated by externally added antibody","authors":"T. Matsuo, T. Yamamoto, K. Niiyama, N. Yamazaki, T. Ishida, H. Kiwada, Y. Shinohara, M. Kataoka","doi":"10.1109/MHS.2007.4420829","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420829","url":null,"abstract":"Pf3 coat protein, a protein coded by bacteriophage Pf3, is a short membrane spanning protein consisting from 44 amino acid residues. Its mutant protein PO-3L, having three additional Leu residues in its transmembrane region, was demonstrated to be spontaneously inserted into large unilamellar vesicle (LUV), even in the absence of protein machineries or membrane potential. However, detailed interaction manner of peptide with LUV are not yet fully understood. In the present study, we designed two PO derivatives of 3L-DR and 3L-RD in which His, T7 or Myc tags are incorporated. These mutants were expressed in bacteria and homogeneously purified by Ni Sepharose column chromatography. When they were incubated with preformed LUV, their spontaneous insertion into LUV was observed. Because both N- and C-terminal of newly designed peptides contain either T7 or Myc tag, even when one of N- or C-terminus was digested with trypsin, resulting truncated peptide could be detected using antibody against the tags present in the opposite C- or N-terminal. Using this property of the peptides, topologies of newly designed peptides embedded into LUV were clearly determined. Furthermore, we applied the membrane anchoring property of 3L-DR for development of a protein A derivative which could be spontaneously inserted into liposomal membrane. When parental small IgG binding unit of protein A was mixed with liposome, its binding to the liposome was not observed, however, its fusion peptide with 3L-DR was spontaneously incorporated into liposomal membrane. Furthermore, when antiserum was added to this liposome, significant binding of IgG to the liposome was observed. Thus, by using protein A derivative fused to the 3L-DR, we succeeded in the preparation of novel liposome of which surface could be modifiable with externally added antibodies.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125310393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coarse and fine motion micro robot with six degrees of freedom","authors":"S. Kusunoki, A. Torii, K. Doki, A. Ueda","doi":"10.1109/MHS.2007.4420826","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420826","url":null,"abstract":"Displacement of a micro robot is described theoretically and experimentally. The structure of the robot is a Stewart-platform using six stacked-type piezoelectric actuators. The robot, which can realize a precise motion, is developed for a flexible conveying system, such as a desktop manufacturing system. The robot is used in a micro/nano system which requires a nanometer resolution. We describe the principle of the motion and demonstrate the self-propelled motion and the precise motion.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131558511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variable proximity multiple exposures in SR Lithography for fabrication of 3D micro/nanostructures","authors":"F. Kato, S. Sugiyama","doi":"10.1109/MHS.2007.4420854","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420854","url":null,"abstract":"The variable proximity multi exposure technique in SR lithography is introduced. This technique provides to fabricate micro/nano gratings include Sub-wavelength gratings. The sub-wavelength gratings for antireflection were fabricated using this technique. Fresnel and Fraunhofer intensity distribution simulation for estimate the properly gap were performed. The simulation was done by the slit width of 150 nm and 250 nm, and the proximity gap was from 0 up to 100 mum. On the other hand, evaluation of proximity gap effect with conventional exposure was performed. We found the appropriate value of the gap for obtaining high contrast, and the gap for provide a taper. The experiment results were in agreement with the calculated result. T Then the multiple exposure with appropriate gap were performed and we were able to obtain the three dimensional sub-wavelength structures.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116518796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an Electrostatic Micro Transportation System (MTS) with Strider-like Movement of Micro Carts","authors":"D. Dao, P. H. Pham, S. Sugiyama","doi":"10.1109/MHS.2007.4420876","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420876","url":null,"abstract":"This paper presents a novel micro transportation system (MTS), which can drive micro carts by utilizing electrostatic actuator and ratchet mechanism. The micro cart, which has driving-wings and anti-reverse-wings attached to its central 'backbone', is driven by electrostatic actuator through the ratchet racks in the direction perpendiculars to the movement direction of the cart. The driving wings of the cart play a role like the legs of a water strider. The MTS was fabricated from SOI wafer by using only one mask. The movement of the micro cart has been tested with driving frequency ranges from 1 Hz to 20 Hz. The velocity of the micro cart was linearly proportional to the driving frequency, and it matched well with theoretical calculation. The MTS combined with microscopes is expected to be applied in bio-medical analysis for transportation and classification of small samples.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127809282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2D-SPR affinity biosensing in 10 μm wells for multi-scale biosensing","authors":"M. Suzuki, T. Ohshima, Y. Iribe","doi":"10.1109/MHS.2007.4420840","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420840","url":null,"abstract":"We have been developing multi-scale affinity sensor which can be applied from 10 μm (single cell) to 1 mm ranges based on two-dimensional surface plasmon resonance (2D-SPR) imaging. But SPR sensitivity was drastically decreased in 10 μm wells because surface plasmon wave cannot propagate in such micro area. The use of shorter wavelength incident light might improve sensitivity of SPR sensing in micro wells. This paper described improvement of sensitivity and resolution of 2D-SPR imaging in 10 μm microwell array by using silver-based novel sensor chip and 505 nm incident light.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115280555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of IR-LEGO to heat shock mediated-gene induction in targeted single cells of C. elegans","authors":"M. Suzuki, Y. Kamei, S. Yuba, S. Takagi","doi":"10.1109/MHS.2007.4420841","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420841","url":null,"abstract":"Ectopic expression via transgene is powerful means for functional analysis of genes of multicellular organisms in vivo. Use of tissue-specific promoters for ectopic expression is limited by their inherent properties to drive gene expression in a restricted temporal-spatial expression pattern. We have developed a novel microscopic system utilizing an infrared laser, IR-LEGO (infrared-laser evoked gene operator) in order to induce heat shock response efficiently in targeted single cells of living organisms, thereby driving expression of a transgene under the control of a heat shock promoter. The system utilizes IR light because we expect that the IR laser can heat biological tissues much more efficiently and is less harmful to cells compared with a Coumarin dye laser, which was used in previous studies. When applied to living nematode C. elegans worms under appropriate conditions, IR-LEGO induced gene expression effectively and reproducibly in targeted single cells. Heat shock-induced cells underwent subsequent development normally, suggesting that the IR-LEGO-mediated heat shock induction does not cause significant cell damages. The system would enable us to induce gene expression in temporally and spatially regulated manners in any organisms, for which transgenic technology is available.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"35 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123615235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-21 as an Effective Suppressant for IgE-mediated Allergic Hypersensitivity Reactions","authors":"T. Kishida, Y. Hiromura, T. Hama, J. Imanishi, Y. Hisa, O. Mazda","doi":"10.1109/MHS.2007.4420871","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420871","url":null,"abstract":"IgE plays essential roles in the pathogenesis of many allergic disorders including bronchial asthma, allergic rhinitis, atopic dermatitis, and anaphylaxis. If IgE production can be effectively and safely controlled in patients, such procedures may alleviate allergic symptoms, leading to novel therapeutic and prophylactic interventions of allergic diseases. Interleukin-21 (IL-21) is a cytokine produced by activated T cells. It exerts pleiotrophic immunomodulatory functions through acting on various immune cells including T, B, and NK cells. To analyze influence of exogenous IL-21 in vivo, we inserted an expression unit of IL-21 cDNA into an Epstein-Barr virus (EBV)-based artificial chromosome, so that extremely powerful and long-lasting expression of the cytokine can be achieved in vivo in the liver. Peanut anaphylactic mice were established by repetitive immunization with the crude peanut extract (CPE) as an allergen, and they received intravenous administration of the DNA construct through the tail vein under high pressure. As the results, anaphylactic symptoms were completely abrogated by the IL-21 gene treatment, in striking contrast to untreated allergic mice that showed extremely severe systemic disturbance. Serum level of IgE was also drastically suppressed by IL-21 gene treatment. Then we used recombinant IL-21 (rIL-21) to treat anaphylactic as well as allergic rhinitis mice, which also showed significant remission of the diseases. As the mechanisms of the IgE regulation, we found that expression of germ line Cepsiv transcript was suppressed in B cells that were treated with rIL-21 in vitro or in vivo, suggesting that IL-21 effectively suppressed the class switch recombination (CSR) to IgE. The present findings strongly suggest that IL-21 can be used as a novel molecular medicine to control allergy. It was also shown that the EBV-based artificial chromosome provides a useful means to analyze bioactivity in vivo of various genes in mammals, providing a platform to investigate functions of genes as well as to discover promising molecules for therapeutic molecular targeting to treat various disorders.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131330302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantum Computing and Information Acquisition","authors":"T. Tarn, N. Ganesan","doi":"10.1109/MHS.2007.4420911","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420911","url":null,"abstract":"Due to the rapid advances made in Nano-Biotechnology, optical control of molecular dynamics and quantum computation, there is an increasing need to understand the fundamental structure, from the systems theoretical point of view, of the control and observation of quantum mechanical systems for designing advanced sensors and actuators. In this presentation we start with a discussion of the design, synthesis, and control of engineering problems and how the advances of technology helping to solve complex engineering problems. We proceed to discuss the recent trends in quantum computation and quantum computer. Then, we present the differences between the quantum control and measurement problems and the classical control and measurement problems. We conclude with a list of recent applications of quantum phenomena to build new sensors and other systems for information acquisition.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126249724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nondestructive In-situ Strength Analysis of High-strength Metal with Nano-size Microstructure","authors":"Y. Kojima, H. Kimura, K. Tsuji, Y. Akiniwa, K. Tanka","doi":"10.1109/MHS.2007.4420903","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420903","url":null,"abstract":"In-situ neutron diffraction measurement was conducted to investigate the plastic deformation and fatigue damage behavior of ultrafine-grained high-strength Cu processed by equal channel angular pressing, ECAP. The experiments were conducted on the ultrafine-grained samples, as well as large-grained counterparts, under tensile and fatigue loading using RESA of Japan Atomic Energy Agency. The diffraction profiles were analyzed in relation with the applied stress, strain and fatigue damage. The result of the large-grained samples shows that the full width at half maximum, FWHM, of the profile from 311 diffraction plane increases with increasing applied stress and that the FWHM remains large even when the sample is unloaded due to accumulated plastic strain. On the other hand, the result of the ultrafine-grained samples shows that the FWHM decreases to the original value upon unloading even after the plastic deformation. The similar results were observed in the process of low-cycle fatigue. The decrease in the FWHM in the ultrafine-grained sample is attributed to the rearrangement of transgranular plastic strain in ultrafine grains.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120960085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Magnitude and Duration of Cyclic Stretch Affect JNK and p38 Activation in MC3T3-E1 Pre-Osteoblastic Cells","authors":"H. Matsui, N. Fukuno, K. Sasaki, O. Suzuki, T. Kobayashi, S. Tamura","doi":"10.1109/MHS.2007.4420849","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420849","url":null,"abstract":"Although mechanical stress has been shown to regulate bone modeling and remodeling, the mechanisms of bone remodeling after mechanical stress loading are not fully understood. In this study we determined the effects of cyclic mechanical stretch of varying magnitudes and durations on the activation of JNK and p38 in MC3T3-E1 cells. Phosphorylation of both JNK and p38 was observed 5 min after the onset of stretch-load and increased proportionally with elongation. However, a marked increase in the phosphorylation of p38 but not JNK was observed when cells were stretched to 4% elongation. These observations raise the possibility that JNK and p38 play different roles in bone remodeling depending on the magnitude and duration of mechanical stretch.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"152 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127229179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}